Effects of omeprazole and pirenzepine on enterochromaffin-like cells and parietal cells in rat stomach

The purpose of this study was to investigate the mechanism of the regulation of histamine synthesis in enterochromaffin-like cells, chemically and structurally, by treatment with omeprazole and pirenzepine. The ultrastructures of enterochromaffin-like cells and parietal cells were examined in rats t...

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Veröffentlicht in:Journal of gastroenterology 2001-06, Vol.36 (6), p.375-385
Hauptverfasser: Tari, A, Kuruhara, Y, Yonei, Y, Yamauchi, R, Okahara, S, Sumii, K, Kajiyama, G
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container_end_page 385
container_issue 6
container_start_page 375
container_title Journal of gastroenterology
container_volume 36
creator Tari, A
Kuruhara, Y
Yonei, Y
Yamauchi, R
Okahara, S
Sumii, K
Kajiyama, G
description The purpose of this study was to investigate the mechanism of the regulation of histamine synthesis in enterochromaffin-like cells, chemically and structurally, by treatment with omeprazole and pirenzepine. The ultrastructures of enterochromaffin-like cells and parietal cells were examined in rats treated with oral omeprazole (20 mg/kg) or intraperitoneal pirenzepine (1 mg/kg) administration. Serum gastrin concentrations, mRNA levels of H+-K+-ATPase and histidine decarboxylase, and the fundic concentrations of somatostatin and histamine were determined. Pirenzepine treatment suppressed omeprazole-induced increases in serum gastrin levels and mRNA levels of H+-K+-ATPase and histidine decarboxylase. Pirenzepine also decreased omeprazole-induced increases of histamine concentration in fundic mucosa. Pirenzepine elevated somatostatin mRNA level, previously decreased by omeprazole treatment, in fundic mucosa. In the cytoplasm of enterochromaffin-like cells, omeprazole markedly reduced the numbers of vesicles and granules, but significantly increased their diameters, whereas pirenzepine treatment changed neither of these features. The densities and diameters of both vesicles and granules produced by treatment with omeprazole and pirenzepine were between those produced by treatment with omeprazole alone and pirenzepine alone. Omeprazole-induced hypergastrinemia and pirenzepine-induced somatostatin synthesis play important roles not only in histamine synthesis but also in ultrastructural changes in enterochromaffin-like cells.
doi_str_mv 10.1007/s005350170081
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The densities and diameters of both vesicles and granules produced by treatment with omeprazole and pirenzepine were between those produced by treatment with omeprazole alone and pirenzepine alone. 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subjects Animals
Anti-Ulcer Agents - therapeutic use
Blotting, Northern
Drug Therapy, Combination
Enterochromaffin-like Cells - drug effects
H(+)-K(+)-Exchanging ATPase - drug effects
Histamine - blood
Hydrogen-Ion Concentration - drug effects
Male
Omeprazole - therapeutic use
Parietal Cells, Gastric - drug effects
Pirenzepine - therapeutic use
Rats
Rats, Wistar
RNA, Messenger - drug effects
Stomach - chemistry
Stomach - cytology
Stomach - drug effects
title Effects of omeprazole and pirenzepine on enterochromaffin-like cells and parietal cells in rat stomach
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