The stress kit: a new method based on competitive reverse transcriptase-polymerase chain reaction to quantify the expression of human alphaB-crystallin, Hsp27, and Hsp60

We describe a reverse transcriptase-polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins alphaB-crystallin, Hsp27, and Hsp60. The method involves the coamplification of cellular mRNA-derived cDNA with a dilution series of a competitor fr...

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Veröffentlicht in:	Cell stress & chaperones 2000-01, Vol.5 (1), p.30-35
Hauptverfasser:	Bajramović, J J, Geutskens, S B, Bsibsi, M, Boot, M, Hassankhan, R, Verhulst, K C, van Noort, J M
Format:	Artikel
Sprache:	eng
Schlagworte:	Astrocytoma - pathology Binding, Competitive Brain Neoplasms - pathology Chaperonin 60 - genetics Crystallins - genetics DNA, Complementary - genetics Heat-Shock Proteins Hot Temperature HSP27 Heat-Shock Proteins Humans Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Reagent Kits, Diagnostic Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis RNA, Messenger - genetics RNA, Neoplasm - biosynthesis RNA, Neoplasm - genetics Stress, Physiological - genetics Stress, Physiological - metabolism Tumor Cells, Cultured
Online-Zugang:	Volltext
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