Assay of a seric human hexapeptide (HWESAS) using a monoclonal antibody and ELISA
Human serum contains low-molecular-weight growth factors potentiating some in vitro biological effects of IGF-I and IGF-II and recently two peptides were mainly identified: HWESAS and WGHE. In order to determine seric HWESAS concentration, a specific monoclonal antibody against HWESAS was prepared....
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| Veröffentlicht in: | Clinica chimica acta 2000-03, Vol.293 (1), p.89-103 |
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| creator | Capiaumont, Josette Jacob, Christine Sarem, Mahrou Nabet, Pierre Belleville, Francine Dousset, Brigitte |
| description | Human serum contains low-molecular-weight growth factors potentiating some in vitro biological effects of IGF-I and IGF-II and recently two peptides were mainly identified: HWESAS and WGHE. In order to determine seric HWESAS concentration, a specific monoclonal antibody against HWESAS was prepared. Its specificity was studied by inhibition tests: this antibody cross-reacts with Y–HWESAS, Cys–HWESAS. It does not react with HWESAS when its COOH is blocked, or with HWE, WGHE and tryptophan or with C3f (SSKITHRI
HWESASLLR) which is a fragment of human complement containing HWESAS motif. Its affinity was measured by non competitive enzyme immunoassay (3.89±2.44·10
8 M
−1). Then, this antibody was used in enzyme-linked immunosorbent assay (ELISA) and the preliminary assays were performed to detect HWESAS in serum. In contrast to healthy subjects, patients with chronic renal failure exhibited undetectable concentration of hexapeptide while after successful renal transplantation values increased to reach levels found in healthy subjects and varying according to post-operative evolution. These data are a strong hint that the kidney plays an important role in the production of this hexapeptide and underly the clinical interest of HWESAS detection in renal pathology. |
| doi_str_mv | 10.1016/S0009-8981(99)00221-1 |
| format | Article |
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HWESASLLR) which is a fragment of human complement containing HWESAS motif. Its affinity was measured by non competitive enzyme immunoassay (3.89±2.44·10
8 M
−1). Then, this antibody was used in enzyme-linked immunosorbent assay (ELISA) and the preliminary assays were performed to detect HWESAS in serum. In contrast to healthy subjects, patients with chronic renal failure exhibited undetectable concentration of hexapeptide while after successful renal transplantation values increased to reach levels found in healthy subjects and varying according to post-operative evolution. These data are a strong hint that the kidney plays an important role in the production of this hexapeptide and underly the clinical interest of HWESAS detection in renal pathology.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/S0009-8981(99)00221-1</identifier><identifier>PMID: 10699425</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - isolation & purification ; Antibody Specificity ; Complement C3 - analysis ; Complement C3 - genetics ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Haptens ; Humans ; Hybridomas ; Kidney ; Kidney Failure, Chronic - blood ; Kidney Transplantation ; Low molecular weight growth factor ; Mice ; Mice, Inbred BALB C ; Monoclonal antibody ; Oligopeptides - analysis ; Oligopeptides - chemical synthesis ; Renal Dialysis</subject><ispartof>Clinica chimica acta, 2000-03, Vol.293 (1), p.89-103</ispartof><rights>2000 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-fd2572341720f6e85663710ed1298d4a7b3f1fc15e23a6b6610375cca1b0ad043</citedby><cites>FETCH-LOGICAL-c361t-fd2572341720f6e85663710ed1298d4a7b3f1fc15e23a6b6610375cca1b0ad043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0009-8981(99)00221-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10699425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Capiaumont, Josette</creatorcontrib><creatorcontrib>Jacob, Christine</creatorcontrib><creatorcontrib>Sarem, Mahrou</creatorcontrib><creatorcontrib>Nabet, Pierre</creatorcontrib><creatorcontrib>Belleville, Francine</creatorcontrib><creatorcontrib>Dousset, Brigitte</creatorcontrib><title>Assay of a seric human hexapeptide (HWESAS) using a monoclonal antibody and ELISA</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Human serum contains low-molecular-weight growth factors potentiating some in vitro biological effects of IGF-I and IGF-II and recently two peptides were mainly identified: HWESAS and WGHE. In order to determine seric HWESAS concentration, a specific monoclonal antibody against HWESAS was prepared. Its specificity was studied by inhibition tests: this antibody cross-reacts with Y–HWESAS, Cys–HWESAS. It does not react with HWESAS when its COOH is blocked, or with HWE, WGHE and tryptophan or with C3f (SSKITHRI
HWESASLLR) which is a fragment of human complement containing HWESAS motif. Its affinity was measured by non competitive enzyme immunoassay (3.89±2.44·10
8 M
−1). Then, this antibody was used in enzyme-linked immunosorbent assay (ELISA) and the preliminary assays were performed to detect HWESAS in serum. In contrast to healthy subjects, patients with chronic renal failure exhibited undetectable concentration of hexapeptide while after successful renal transplantation values increased to reach levels found in healthy subjects and varying according to post-operative evolution. These data are a strong hint that the kidney plays an important role in the production of this hexapeptide and underly the clinical interest of HWESAS detection in renal pathology.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - isolation & purification</subject><subject>Antibody Specificity</subject><subject>Complement C3 - analysis</subject><subject>Complement C3 - genetics</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Haptens</subject><subject>Humans</subject><subject>Hybridomas</subject><subject>Kidney</subject><subject>Kidney Failure, Chronic - blood</subject><subject>Kidney Transplantation</subject><subject>Low molecular weight growth factor</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Monoclonal antibody</subject><subject>Oligopeptides - analysis</subject><subject>Oligopeptides - chemical synthesis</subject><subject>Renal Dialysis</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFO4zAQhi3EaillHwHkEyqHsB47ceITqlChSJVWq7Lao-XYEzBK4hIniL49KUWIG6eZkb6Z0f8RcgrsEhjI32vGmEoKVcBMqQvGOIcEDsgEilwkIlX8kEw-kSNyHOPTOKZMwk9yBEwqlfJsQv7OYzRbGipqaMTOW_o4NKalj_hqNrjpvUM6W_5frOfrCzpE3z6MYBPaYOvQmpqatvdlcNuxcXSxulvPT8iPytQRf33UKfl3s7i_XiarP7d31_NVYoWEPqkcz3IuUsg5qyQWmZQiB4YOuCpcavJSVFBZyJALI0spgYk8s9ZAyYxjqZiS8_3dTReeB4y9bny0WNemxTBEnbNdQlGMYLYHbRdi7LDSm843pttqYHrnUr-71DtRWin97lLDuHf28WAoG3RftvbyRuBqD-AY88Vjp6P12Fp0vkPbaxf8Ny_eANpCgQ0</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Capiaumont, Josette</creator><creator>Jacob, Christine</creator><creator>Sarem, Mahrou</creator><creator>Nabet, Pierre</creator><creator>Belleville, Francine</creator><creator>Dousset, Brigitte</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Assay of a seric human hexapeptide (HWESAS) using a monoclonal antibody and ELISA</title><author>Capiaumont, Josette ; Jacob, Christine ; Sarem, Mahrou ; Nabet, Pierre ; Belleville, Francine ; Dousset, Brigitte</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-fd2572341720f6e85663710ed1298d4a7b3f1fc15e23a6b6610375cca1b0ad043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - isolation & purification</topic><topic>Antibody Specificity</topic><topic>Complement C3 - analysis</topic><topic>Complement C3 - genetics</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Haptens</topic><topic>Humans</topic><topic>Hybridomas</topic><topic>Kidney</topic><topic>Kidney Failure, Chronic - blood</topic><topic>Kidney Transplantation</topic><topic>Low molecular weight growth factor</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Monoclonal antibody</topic><topic>Oligopeptides - analysis</topic><topic>Oligopeptides - chemical synthesis</topic><topic>Renal Dialysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Capiaumont, Josette</creatorcontrib><creatorcontrib>Jacob, Christine</creatorcontrib><creatorcontrib>Sarem, Mahrou</creatorcontrib><creatorcontrib>Nabet, Pierre</creatorcontrib><creatorcontrib>Belleville, Francine</creatorcontrib><creatorcontrib>Dousset, Brigitte</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Capiaumont, Josette</au><au>Jacob, Christine</au><au>Sarem, Mahrou</au><au>Nabet, Pierre</au><au>Belleville, Francine</au><au>Dousset, Brigitte</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assay of a seric human hexapeptide (HWESAS) using a monoclonal antibody and ELISA</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>293</volume><issue>1</issue><spage>89</spage><epage>103</epage><pages>89-103</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Human serum contains low-molecular-weight growth factors potentiating some in vitro biological effects of IGF-I and IGF-II and recently two peptides were mainly identified: HWESAS and WGHE. In order to determine seric HWESAS concentration, a specific monoclonal antibody against HWESAS was prepared. Its specificity was studied by inhibition tests: this antibody cross-reacts with Y–HWESAS, Cys–HWESAS. It does not react with HWESAS when its COOH is blocked, or with HWE, WGHE and tryptophan or with C3f (SSKITHRI
HWESASLLR) which is a fragment of human complement containing HWESAS motif. Its affinity was measured by non competitive enzyme immunoassay (3.89±2.44·10
8 M
−1). Then, this antibody was used in enzyme-linked immunosorbent assay (ELISA) and the preliminary assays were performed to detect HWESAS in serum. In contrast to healthy subjects, patients with chronic renal failure exhibited undetectable concentration of hexapeptide while after successful renal transplantation values increased to reach levels found in healthy subjects and varying according to post-operative evolution. These data are a strong hint that the kidney plays an important role in the production of this hexapeptide and underly the clinical interest of HWESAS detection in renal pathology.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10699425</pmid><doi>10.1016/S0009-8981(99)00221-1</doi><tpages>15</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - isolation & purification Antibody Specificity Complement C3 - analysis Complement C3 - genetics ELISA Enzyme-Linked Immunosorbent Assay Haptens Humans Hybridomas Kidney Kidney Failure, Chronic - blood Kidney Transplantation Low molecular weight growth factor Mice Mice, Inbred BALB C Monoclonal antibody Oligopeptides - analysis Oligopeptides - chemical synthesis Renal Dialysis |
| title | Assay of a seric human hexapeptide (HWESAS) using a monoclonal antibody and ELISA |
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