Age‐dependent transformation frequency in elite wheat varieties
Wheat is a major world crop and as such is a primary target for improvement of agronomic characteristics via genetic engineering. Optimization of transformation is essential in order to overcome the relatively low transformation frequencies encountered with wheat. Transformation of elite wheat varie...
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| Veröffentlicht in: | Journal of experimental botany 2001-04, Vol.52 (357), p.857-863 |
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| container_title | Journal of experimental botany |
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| creator | Pastori, Gabriela M. Wilkinson, Mark D. Steele, Sue H. Sparks, Caroline A. Jones, Huw D. Parry, Martin A.J. |
| description | Wheat is a major world crop and as such is a primary target for improvement of agronomic characteristics via genetic engineering. Optimization of transformation is essential in order to overcome the relatively low transformation frequencies encountered with wheat. Transformation of elite wheat varieties is not always successful due to variability in regeneration and transformation frequencies between varieties. In this work, two elite wheat varieties with a relatively high embryogenic capacity were transformed by particle bombardment. A strong correlation between transformation frequency and the age of wheat donor plants was observed in both varieties. The mean transformation frequency rose from 0.7% to 5% when using immature embryos from old and young donor plants, respectively. This was observed in both varieties, the best bombardments achieving up to 7.3% frequency. Using explants at an optimal developmental stage from donor plants grown under environmentally‐controlled conditions has improved the reproducibility of transformation efficiency of elite wheat varieties and leads to the production of apparently phenotypically normal, fertile, transgenic plants. |
| doi_str_mv | 10.1093/jexbot/52.357.857 |
| format | Article |
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Optimization of transformation is essential in order to overcome the relatively low transformation frequencies encountered with wheat. Transformation of elite wheat varieties is not always successful due to variability in regeneration and transformation frequencies between varieties. In this work, two elite wheat varieties with a relatively high embryogenic capacity were transformed by particle bombardment. A strong correlation between transformation frequency and the age of wheat donor plants was observed in both varieties. The mean transformation frequency rose from 0.7% to 5% when using immature embryos from old and young donor plants, respectively. This was observed in both varieties, the best bombardments achieving up to 7.3% frequency. Using explants at an optimal developmental stage from donor plants grown under environmentally‐controlled conditions has improved the reproducibility of transformation efficiency of elite wheat varieties and leads to the production of apparently phenotypically normal, fertile, transgenic plants.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jexbot/52.357.857</identifier><identifier>PMID: 11413223</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Agronomy. Soil science and plant productions ; Bar gene ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Cadenzas ; Callus ; days post‐anthesis ; DNA Primers ; DPA ; elite wheat varieties ; Embryos ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic engineering applications ; Genetic technics ; Genetics and breeding of economic plants ; GUS ; Immatures ; l‐phosphinothricin ; l‐PPT ; Methods Papers ; Methods. Procedures. Technologies ; photosynthetic photon flux density ; Plant breeding: fundamental aspects and methodology ; Plant cells ; Plants ; Polymerase chain reaction ; PPFD ; Regeneration ; transformation frequency ; Transformation, Genetic ; Transgenic animals and transgenic plants ; Transgenic plants ; Triticum - genetics ; uidA gene ; Wheat ; wheat transformation ; β‐glucuronidase</subject><ispartof>Journal of experimental botany, 2001-04, Vol.52 (357), p.857-863</ispartof><rights>Oxford University Press 2001</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-981e9fbc68444963a59c083d6cbe137587db6daacb3330ecbca042e1be6d4f203</citedby><cites>FETCH-LOGICAL-c386t-981e9fbc68444963a59c083d6cbe137587db6daacb3330ecbca042e1be6d4f203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23696708$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23696708$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1037583$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11413223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pastori, Gabriela M.</creatorcontrib><creatorcontrib>Wilkinson, Mark D.</creatorcontrib><creatorcontrib>Steele, Sue H.</creatorcontrib><creatorcontrib>Sparks, Caroline A.</creatorcontrib><creatorcontrib>Jones, Huw D.</creatorcontrib><creatorcontrib>Parry, Martin A.J.</creatorcontrib><title>Age‐dependent transformation frequency in elite wheat varieties</title><title>Journal of experimental botany</title><addtitle>J. Exp. Bot</addtitle><description>Wheat is a major world crop and as such is a primary target for improvement of agronomic characteristics via genetic engineering. Optimization of transformation is essential in order to overcome the relatively low transformation frequencies encountered with wheat. Transformation of elite wheat varieties is not always successful due to variability in regeneration and transformation frequencies between varieties. In this work, two elite wheat varieties with a relatively high embryogenic capacity were transformed by particle bombardment. A strong correlation between transformation frequency and the age of wheat donor plants was observed in both varieties. The mean transformation frequency rose from 0.7% to 5% when using immature embryos from old and young donor plants, respectively. This was observed in both varieties, the best bombardments achieving up to 7.3% frequency. Using explants at an optimal developmental stage from donor plants grown under environmentally‐controlled conditions has improved the reproducibility of transformation efficiency of elite wheat varieties and leads to the production of apparently phenotypically normal, fertile, transgenic plants.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Bar gene</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cadenzas</subject><subject>Callus</subject><subject>days post‐anthesis</subject><subject>DNA Primers</subject><subject>DPA</subject><subject>elite wheat varieties</subject><subject>Embryos</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>Genetic technics</subject><subject>Genetics and breeding of economic plants</subject><subject>GUS</subject><subject>Immatures</subject><subject>l‐phosphinothricin</subject><subject>l‐PPT</subject><subject>Methods Papers</subject><subject>Methods. Procedures. Technologies</subject><subject>photosynthetic photon flux density</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant cells</subject><subject>Plants</subject><subject>Polymerase chain reaction</subject><subject>PPFD</subject><subject>Regeneration</subject><subject>transformation frequency</subject><subject>Transformation, Genetic</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>Triticum - genetics</subject><subject>uidA gene</subject><subject>Wheat</subject><subject>wheat transformation</subject><subject>β‐glucuronidase</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM9u00AQh1eIiqaBB-AA8gH15nT2v31MI0orVeJSUMRltV6PYYNjh90NtDcegWfkSdjIUelpDr9vfpr5CHlNYUGh5hcbvG_GdCHZgku9qKR-RmZUKCiZ4PQ5mQEwVkIt9Sk5i3EDABKkfEFOKRWUM8ZnZLn8in9__2lxh0OLQypSsEPsxrC1yY9D0QX8scfBPRR-KLD3CYtf39Cm4qcNHpPH-JKcdLaP-Oo45-TT1fu71XV5-_HDzWp5WzpeqVTWFcW6a5yqhBC14lbWDireKtcg5VpWum1Ua61rOOeArnEWBEPaoGpFx4DPyfnUuwtjPikms_XRYd_bAcd9NBpqQSumM0gn0IUxxoCd2QW_teHBUDAHb2byZiQz2ZvJ3vLO22P5vtli-3_jKCoD746Ajc72XbbkfHzSfHjhgL2ZsE1MY3iMGVe10vndOSmn3MeE94-5Dd-N0rnCXK-_mEu6hs_rq7VZ8X_g_pHO</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Pastori, Gabriela M.</creator><creator>Wilkinson, Mark D.</creator><creator>Steele, Sue H.</creator><creator>Sparks, Caroline A.</creator><creator>Jones, Huw D.</creator><creator>Parry, Martin A.J.</creator><general>Oxford University Press</general><general>OXFORD UNIVERSITY PRESS</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010401</creationdate><title>Age‐dependent transformation frequency in elite wheat varieties</title><author>Pastori, Gabriela M. ; Wilkinson, Mark D. ; Steele, Sue H. ; Sparks, Caroline A. ; Jones, Huw D. ; Parry, Martin A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-981e9fbc68444963a59c083d6cbe137587db6daacb3330ecbca042e1be6d4f203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Bar gene</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cadenzas</topic><topic>Callus</topic><topic>days post‐anthesis</topic><topic>DNA Primers</topic><topic>DPA</topic><topic>elite wheat varieties</topic><topic>Embryos</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>Genetic technics</topic><topic>Genetics and breeding of economic plants</topic><topic>GUS</topic><topic>Immatures</topic><topic>l‐phosphinothricin</topic><topic>l‐PPT</topic><topic>Methods Papers</topic><topic>Methods. Procedures. 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The mean transformation frequency rose from 0.7% to 5% when using immature embryos from old and young donor plants, respectively. This was observed in both varieties, the best bombardments achieving up to 7.3% frequency. Using explants at an optimal developmental stage from donor plants grown under environmentally‐controlled conditions has improved the reproducibility of transformation efficiency of elite wheat varieties and leads to the production of apparently phenotypically normal, fertile, transgenic plants.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>11413223</pmid><doi>10.1093/jexbot/52.357.857</doi><tpages>7</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Bar gene Base Sequence Biological and medical sciences Biotechnology Cadenzas Callus days post‐anthesis DNA Primers DPA elite wheat varieties Embryos Fundamental and applied biological sciences. Psychology Genetic engineering Genetic engineering applications Genetic technics Genetics and breeding of economic plants GUS Immatures l‐phosphinothricin l‐PPT Methods Papers Methods. Procedures. Technologies photosynthetic photon flux density Plant breeding: fundamental aspects and methodology Plant cells Plants Polymerase chain reaction PPFD Regeneration transformation frequency Transformation, Genetic Transgenic animals and transgenic plants Transgenic plants Triticum - genetics uidA gene Wheat wheat transformation β‐glucuronidase |
| title | Age‐dependent transformation frequency in elite wheat varieties |
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