Ontogeny of Steroidogenesis in the Fetal Sheep Gonad
The aim of this study was to determine 1) the time of onset and cellular localization of gene expression for steroidogenic factor-1 (SF-1), steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase/Π5 ,Π4 isomerase (3β-HSD), and the cytochrome P450 enzymes for cholesterol side-ch...
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| Veröffentlicht in: | Biology of reproduction 2001-07, Vol.65 (1), p.216-228 |
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| creator | QUIRKE, Laurel D JUENGEL, Jennifer L TISDALL, David J LUN, Stan HEATH, Derek A MCNATTY, Kenneth P |
| description | The aim of this study was to determine 1) the time of onset and cellular localization of gene expression for steroidogenic
factor-1 (SF-1), steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase/Π5 ,Π4 isomerase (3β-HSD), and the cytochrome P450 enzymes for cholesterol side-chain cleavage (P450 scc ), 17α-hydroxylase (P450 17αOH ), and aromatase (P450 arom ) during gonadal development; and 2) the amount of progesterone, androstenedione, testosterone, and 17β-estradiol present
in the fetal sheep gonad. Fetuses were collected on Days 24, 26, 28, 30, 32, 35, 40, 55, and 75 of gestation, and gene expression
was determined by in situ hybridization. The steroid content of gonads collected on Days 30, 35, 55, and 75 of gestation was
determined by RIA. Developing gonads collected from both male and female fetuses were steroidogenically active around the
time of morphological sexual differentiation. In the female, the steroidogenic cells were initially located at the boundary
of the cortex and medulla but become increasingly restricted to the mesonephric-derived cell streams. In the male, once tubules
were identifiable, steroidogenesis was restricted to the interstitial regions. Interestingly, expression of both SF-1 and
3β-HSD was observed prior to morphological sexual differentiation. In addition, expression of both of these genes was more
widespread than the other genes in both males and females. |
| doi_str_mv | 10.1095/biolreprod65.1.216 |
| format | Article |
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factor-1 (SF-1), steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase/Π5 ,Π4 isomerase (3β-HSD), and the cytochrome P450 enzymes for cholesterol side-chain cleavage (P450 scc ), 17α-hydroxylase (P450 17αOH ), and aromatase (P450 arom ) during gonadal development; and 2) the amount of progesterone, androstenedione, testosterone, and 17β-estradiol present
in the fetal sheep gonad. Fetuses were collected on Days 24, 26, 28, 30, 32, 35, 40, 55, and 75 of gestation, and gene expression
was determined by in situ hybridization. The steroid content of gonads collected on Days 30, 35, 55, and 75 of gestation was
determined by RIA. Developing gonads collected from both male and female fetuses were steroidogenically active around the
time of morphological sexual differentiation. In the female, the steroidogenic cells were initially located at the boundary
of the cortex and medulla but become increasingly restricted to the mesonephric-derived cell streams. In the male, once tubules
were identifiable, steroidogenesis was restricted to the interstitial regions. Interestingly, expression of both SF-1 and
3β-HSD was observed prior to morphological sexual differentiation. In addition, expression of both of these genes was more
widespread than the other genes in both males and females.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod65.1.216</identifier><identifier>PMID: 11420243</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>3-Hydroxysteroid Dehydrogenases - biosynthesis ; 3-Hydroxysteroid Dehydrogenases - genetics ; Animals ; Biological and medical sciences ; Cloning, Molecular ; Cytochrome P-450 Enzyme System - biosynthesis ; DNA-Binding Proteins - biosynthesis ; Embryonic and Fetal Development - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Fushi Tarazu Transcription Factors ; Homeodomain Proteins ; Immunohistochemistry ; In Situ Hybridization ; Male ; Ovary - embryology ; Ovary - metabolism ; Phosphoproteins - biosynthesis ; Receptors, Cytoplasmic and Nuclear ; RNA, Messenger - biosynthesis ; Sexual differentiation and maturation. Puberty. Climacterium ; Sheep ; Steroidogenic Factor 1 ; Steroids - biosynthesis ; Testis - embryology ; Testis - metabolism ; Transcription Factors - biosynthesis ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2001-07, Vol.65 (1), p.216-228</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1130582$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11420243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>QUIRKE, Laurel D</creatorcontrib><creatorcontrib>JUENGEL, Jennifer L</creatorcontrib><creatorcontrib>TISDALL, David J</creatorcontrib><creatorcontrib>LUN, Stan</creatorcontrib><creatorcontrib>HEATH, Derek A</creatorcontrib><creatorcontrib>MCNATTY, Kenneth P</creatorcontrib><title>Ontogeny of Steroidogenesis in the Fetal Sheep Gonad</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The aim of this study was to determine 1) the time of onset and cellular localization of gene expression for steroidogenic
factor-1 (SF-1), steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase/Π5 ,Π4 isomerase (3β-HSD), and the cytochrome P450 enzymes for cholesterol side-chain cleavage (P450 scc ), 17α-hydroxylase (P450 17αOH ), and aromatase (P450 arom ) during gonadal development; and 2) the amount of progesterone, androstenedione, testosterone, and 17β-estradiol present
in the fetal sheep gonad. Fetuses were collected on Days 24, 26, 28, 30, 32, 35, 40, 55, and 75 of gestation, and gene expression
was determined by in situ hybridization. The steroid content of gonads collected on Days 30, 35, 55, and 75 of gestation was
determined by RIA. Developing gonads collected from both male and female fetuses were steroidogenically active around the
time of morphological sexual differentiation. In the female, the steroidogenic cells were initially located at the boundary
of the cortex and medulla but become increasingly restricted to the mesonephric-derived cell streams. In the male, once tubules
were identifiable, steroidogenesis was restricted to the interstitial regions. Interestingly, expression of both SF-1 and
3β-HSD was observed prior to morphological sexual differentiation. In addition, expression of both of these genes was more
widespread than the other genes in both males and females.</description><subject>3-Hydroxysteroid Dehydrogenases - biosynthesis</subject><subject>3-Hydroxysteroid Dehydrogenases - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Cytochrome P-450 Enzyme System - biosynthesis</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>Embryonic and Fetal Development - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fushi Tarazu Transcription Factors</subject><subject>Homeodomain Proteins</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Ovary - embryology</subject><subject>Ovary - metabolism</subject><subject>Phosphoproteins - biosynthesis</subject><subject>Receptors, Cytoplasmic and Nuclear</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Sexual differentiation and maturation. Puberty. Climacterium</subject><subject>Sheep</subject><subject>Steroidogenic Factor 1</subject><subject>Steroids - biosynthesis</subject><subject>Testis - embryology</subject><subject>Testis - metabolism</subject><subject>Transcription Factors - biosynthesis</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0E1Lw0AQBuBFFFs__oAHyUG9pc7uJJvkKMVWodBD9bxssrPNSj5qNiX03xtpBU_DDA8vvMPYHYcZhyx-zl1bdbTrWiPjGZ8JLs_YlMciCxMh03M2BQAZIkqcsCvvvwB4hAIv2YTzSICIcMqiddO3W2oOQWuDTU9d68zvTt75wDVBX1KwoF5XwaYk2gXLttHmhl1YXXm6Pc1r9rl4_Zi_hav18n3-sgpLIWUfIk-txbQoDJfGRqlNZJ7LJC2MoQy5AAKUkI1nm4O1aWLRFqjzPJHAOVq8Zk_H3LHj9558r2rnC6oq3VC79yqBDDMuYYT3J7jPazJq17ladwf113MEDyegfaEr2-mmcP6fQ4hTMbLHIyvdthxcR8rXuqrGVFTDMMhYcTW-GX8AAIxvtg</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>QUIRKE, Laurel D</creator><creator>JUENGEL, Jennifer L</creator><creator>TISDALL, David J</creator><creator>LUN, Stan</creator><creator>HEATH, Derek A</creator><creator>MCNATTY, Kenneth P</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>Ontogeny of Steroidogenesis in the Fetal Sheep Gonad</title><author>QUIRKE, Laurel D ; JUENGEL, Jennifer L ; TISDALL, David J ; LUN, Stan ; HEATH, Derek A ; MCNATTY, Kenneth P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h266t-318ff38ccd16df48f76bb678cdde93120e03609f76fb0ff87f3fc3abb760113f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>3-Hydroxysteroid Dehydrogenases - biosynthesis</topic><topic>3-Hydroxysteroid Dehydrogenases - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Cytochrome P-450 Enzyme System - biosynthesis</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>Embryonic and Fetal Development - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fushi Tarazu Transcription Factors</topic><topic>Homeodomain Proteins</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Ovary - embryology</topic><topic>Ovary - metabolism</topic><topic>Phosphoproteins - biosynthesis</topic><topic>Receptors, Cytoplasmic and Nuclear</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Sexual differentiation and maturation. Puberty. Climacterium</topic><topic>Sheep</topic><topic>Steroidogenic Factor 1</topic><topic>Steroids - biosynthesis</topic><topic>Testis - embryology</topic><topic>Testis - metabolism</topic><topic>Transcription Factors - biosynthesis</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>QUIRKE, Laurel D</creatorcontrib><creatorcontrib>JUENGEL, Jennifer L</creatorcontrib><creatorcontrib>TISDALL, David J</creatorcontrib><creatorcontrib>LUN, Stan</creatorcontrib><creatorcontrib>HEATH, Derek A</creatorcontrib><creatorcontrib>MCNATTY, Kenneth P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>QUIRKE, Laurel D</au><au>JUENGEL, Jennifer L</au><au>TISDALL, David J</au><au>LUN, Stan</au><au>HEATH, Derek A</au><au>MCNATTY, Kenneth P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ontogeny of Steroidogenesis in the Fetal Sheep Gonad</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>65</volume><issue>1</issue><spage>216</spage><epage>228</epage><pages>216-228</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The aim of this study was to determine 1) the time of onset and cellular localization of gene expression for steroidogenic
factor-1 (SF-1), steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase/Π5 ,Π4 isomerase (3β-HSD), and the cytochrome P450 enzymes for cholesterol side-chain cleavage (P450 scc ), 17α-hydroxylase (P450 17αOH ), and aromatase (P450 arom ) during gonadal development; and 2) the amount of progesterone, androstenedione, testosterone, and 17β-estradiol present
in the fetal sheep gonad. Fetuses were collected on Days 24, 26, 28, 30, 32, 35, 40, 55, and 75 of gestation, and gene expression
was determined by in situ hybridization. The steroid content of gonads collected on Days 30, 35, 55, and 75 of gestation was
determined by RIA. Developing gonads collected from both male and female fetuses were steroidogenically active around the
time of morphological sexual differentiation. In the female, the steroidogenic cells were initially located at the boundary
of the cortex and medulla but become increasingly restricted to the mesonephric-derived cell streams. In the male, once tubules
were identifiable, steroidogenesis was restricted to the interstitial regions. Interestingly, expression of both SF-1 and
3β-HSD was observed prior to morphological sexual differentiation. In addition, expression of both of these genes was more
widespread than the other genes in both males and females.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>11420243</pmid><doi>10.1095/biolreprod65.1.216</doi><tpages>13</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2001-07, Vol.65 (1), p.216-228 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_70939160 |
| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | 3-Hydroxysteroid Dehydrogenases - biosynthesis 3-Hydroxysteroid Dehydrogenases - genetics Animals Biological and medical sciences Cloning, Molecular Cytochrome P-450 Enzyme System - biosynthesis DNA-Binding Proteins - biosynthesis Embryonic and Fetal Development - physiology Female Fundamental and applied biological sciences. Psychology Fushi Tarazu Transcription Factors Homeodomain Proteins Immunohistochemistry In Situ Hybridization Male Ovary - embryology Ovary - metabolism Phosphoproteins - biosynthesis Receptors, Cytoplasmic and Nuclear RNA, Messenger - biosynthesis Sexual differentiation and maturation. Puberty. Climacterium Sheep Steroidogenic Factor 1 Steroids - biosynthesis Testis - embryology Testis - metabolism Transcription Factors - biosynthesis Vertebrates: reproduction |
| title | Ontogeny of Steroidogenesis in the Fetal Sheep Gonad |
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