Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells
We used mRNA subtraction of differentiated and dedifferentiated smooth muscle cells (SMCs) to reveal the molecular mechanisms underlying the phenotypic modulation of SMCs. With this approach, we found that a 10 kb mRNA encoding a homotypic cell adhesion molecule, cadherin 6B, was strongly expressed...
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| Veröffentlicht in: | FEBS letters 2000-03, Vol.469 (1), p.67-71 |
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| creator | Chimori, Yoshihiro Hayashi, Ken’ichiro Kimura, Kazuhiro Nishida, Wataru Funahashi, Shin-ichi Miyata, Shoji Shimane, Miyuki Matsuzawa, Yuji Sobue, Kenji |
| description | We used mRNA subtraction of differentiated and dedifferentiated smooth muscle cells (SMCs) to reveal the molecular mechanisms underlying the phenotypic modulation of SMCs. With this approach, we found that a 10 kb mRNA encoding a homotypic cell adhesion molecule, cadherin 6B, was strongly expressed in differentiated vascular and visceral SMCs, but not in the dedifferentiated SMCs derived from them. In vivo, cadherin 6B was expressed in vascular and visceral SMCs, in addition to brain, spinal cord, retina and kidney, at a late stage of chicken embryonic development. These results suggest that cadherin 6B is a novel molecular marker for vascular and visceral SMC phenotypes and is involved in the late differentiation of SMCs. |
| doi_str_mv | 10.1016/S0014-5793(00)01220-5 |
| format | Article |
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With this approach, we found that a 10 kb mRNA encoding a homotypic cell adhesion molecule, cadherin 6B, was strongly expressed in differentiated vascular and visceral SMCs, but not in the dedifferentiated SMCs derived from them. In vivo, cadherin 6B was expressed in vascular and visceral SMCs, in addition to brain, spinal cord, retina and kidney, at a late stage of chicken embryonic development. These results suggest that cadherin 6B is a novel molecular marker for vascular and visceral SMC phenotypes and is involved in the late differentiation of SMCs.</description><subject>Animals</subject><subject>Biomarkers</subject><subject>Cadherin 6B</subject><subject>Cadherins - genetics</subject><subject>Cadherins - metabolism</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression Regulation, Developmental</subject><subject>In Situ Hybridization</subject><subject>mRNA subtraction</subject><subject>Muscle, Smooth - metabolism</subject><subject>Oligonucleotides, Antisense</subject><subject>Phenotype</subject><subject>Phenotypic modulation</subject><subject>RNA, Messenger - metabolism</subject><subject>Smooth muscle</subject><subject>Viscera - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P3DAQhq0KVBboT2jlEyqH0HEcr5NTBYiPSkhUAi69WI49qzVy4tROtt1_T7JBFbdyGWs877wzeoaQzwzOGLDltwcAVmRCVvwrwCmwPIdMfCALVkqe8WJZ7pHFP8kBOUzpGca8ZNVHcsBAQilFuSC_fq6xDf22w8xih63Ftqf4t4uYkgstDStqtF1jdC1dXtAxbnQyg9eR6tbSjUsGo_Y0NSH0a9oMyXikBr1Px2R_pX3CT6_vEXm6vnq8vM3u7m9-XJ7fZaaQXGRVbkupl9yg4RxASit4XekxFjbXVud1IQvkeiVlZYTNodRYcKw52hxrg_yInMy-XQy_B0y9aqatvNcthiEpCRWXTMAoFLPQxJBSxJXqomt03CoGaoKqdlDVREwBqB1UJca-L68DhrpB-6ZrpjgKbmfBH-dx-z5XdX11ke8qU2E6zfg9zfo-W-FIbOMwqmQctgati2h6ZYP7z7YvjyObew</recordid><startdate>20000303</startdate><enddate>20000303</enddate><creator>Chimori, Yoshihiro</creator><creator>Hayashi, Ken’ichiro</creator><creator>Kimura, Kazuhiro</creator><creator>Nishida, Wataru</creator><creator>Funahashi, Shin-ichi</creator><creator>Miyata, Shoji</creator><creator>Shimane, Miyuki</creator><creator>Matsuzawa, Yuji</creator><creator>Sobue, Kenji</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000303</creationdate><title>Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells</title><author>Chimori, Yoshihiro ; Hayashi, Ken’ichiro ; Kimura, Kazuhiro ; Nishida, Wataru ; Funahashi, Shin-ichi ; Miyata, Shoji ; Shimane, Miyuki ; Matsuzawa, Yuji ; Sobue, Kenji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4735-92d87a63cec330077d53b9ad534d2ada2b474e3af779c5d208ae43eb3ed2ebce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biomarkers</topic><topic>Cadherin 6B</topic><topic>Cadherins - genetics</topic><topic>Cadherins - metabolism</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Expression Regulation, Developmental</topic><topic>In Situ Hybridization</topic><topic>mRNA subtraction</topic><topic>Muscle, Smooth - metabolism</topic><topic>Oligonucleotides, Antisense</topic><topic>Phenotype</topic><topic>Phenotypic modulation</topic><topic>RNA, Messenger - metabolism</topic><topic>Smooth muscle</topic><topic>Viscera - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chimori, Yoshihiro</creatorcontrib><creatorcontrib>Hayashi, Ken’ichiro</creatorcontrib><creatorcontrib>Kimura, Kazuhiro</creatorcontrib><creatorcontrib>Nishida, Wataru</creatorcontrib><creatorcontrib>Funahashi, Shin-ichi</creatorcontrib><creatorcontrib>Miyata, Shoji</creatorcontrib><creatorcontrib>Shimane, Miyuki</creatorcontrib><creatorcontrib>Matsuzawa, Yuji</creatorcontrib><creatorcontrib>Sobue, Kenji</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chimori, Yoshihiro</au><au>Hayashi, Ken’ichiro</au><au>Kimura, Kazuhiro</au><au>Nishida, Wataru</au><au>Funahashi, Shin-ichi</au><au>Miyata, Shoji</au><au>Shimane, Miyuki</au><au>Matsuzawa, Yuji</au><au>Sobue, Kenji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2000-03-03</date><risdate>2000</risdate><volume>469</volume><issue>1</issue><spage>67</spage><epage>71</epage><pages>67-71</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We used mRNA subtraction of differentiated and dedifferentiated smooth muscle cells (SMCs) to reveal the molecular mechanisms underlying the phenotypic modulation of SMCs. With this approach, we found that a 10 kb mRNA encoding a homotypic cell adhesion molecule, cadherin 6B, was strongly expressed in differentiated vascular and visceral SMCs, but not in the dedifferentiated SMCs derived from them. In vivo, cadherin 6B was expressed in vascular and visceral SMCs, in addition to brain, spinal cord, retina and kidney, at a late stage of chicken embryonic development. These results suggest that cadherin 6B is a novel molecular marker for vascular and visceral SMC phenotypes and is involved in the late differentiation of SMCs.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10708758</pmid><doi>10.1016/S0014-5793(00)01220-5</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Biomarkers Cadherin 6B Cadherins - genetics Cadherins - metabolism Cell Differentiation Cells, Cultured Chick Embryo Fluorescent Antibody Technique Gene Expression Regulation, Developmental In Situ Hybridization mRNA subtraction Muscle, Smooth - metabolism Oligonucleotides, Antisense Phenotype Phenotypic modulation RNA, Messenger - metabolism Smooth muscle Viscera - metabolism |
| title | Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells |
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