Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice
Objective: To determine the long-term survival of frozen-thawed human ovarian tissue as xenografts in severe-combined-immunodeficiency (SCID) mice. Design: Animal study. Setting: Animal and laboratory facilities at an academic center. Patient(s): Ovarian tissue obtained from a 27-year-old woman. Int...
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| Veröffentlicht in: | Fertility and sterility 2000-03, Vol.73 (3), p.599-603 |
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| creator | Oktay, Kutluk Newton, Helen Gosden, Roger G |
| description | Objective: To determine the long-term survival of frozen-thawed human ovarian tissue as xenografts in severe-combined-immunodeficiency (SCID) mice.
Design: Animal study.
Setting: Animal and laboratory facilities at an academic center.
Patient(s): Ovarian tissue obtained from a 27-year-old woman.
Intervention(s): Grafting of frozen-thawed ovarian tissue in SCID mice for 22 weeks.
Main Outcome Measure(s): Follicle counts and growth by morphology and PCNA staining in frozen-thawed grafts and fresh controls.
Result(s): All three grafts were recovered intact after 22 weeks. Their stroma was devoid of necrotic cells and contained healthy follicles. The ratio of primordial-total follicles decreased significantly after grafting (0.94 ± 0.02 to 0.87 ± 0.01, control vs. grafting). Compared with controls, after 22 weeks of grafting, a higher percentage of follicles had initiated growth (5.6 ± 2.4 vs. 12.5 ± 1.9), but there was still a significant number of primordial follicles/graft (75 ± 6.8). Follicle stages were similar between two groups; only primordial and one-layer follicles were seen in the xenografts. In the controls, except for one two-layer follicle, the most advanced follicle was at the one-layer stage.
Conclusion(s): Human primordial follicles survive freeze-thaw and long-term xenografting procedures and retain their capacity to initiate growth. These findings encourage future attempts for human autologous ovarian transplantation. |
| doi_str_mv | 10.1016/S0015-0282(99)00548-8 |
| format | Article |
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Design: Animal study.
Setting: Animal and laboratory facilities at an academic center.
Patient(s): Ovarian tissue obtained from a 27-year-old woman.
Intervention(s): Grafting of frozen-thawed ovarian tissue in SCID mice for 22 weeks.
Main Outcome Measure(s): Follicle counts and growth by morphology and PCNA staining in frozen-thawed grafts and fresh controls.
Result(s): All three grafts were recovered intact after 22 weeks. Their stroma was devoid of necrotic cells and contained healthy follicles. The ratio of primordial-total follicles decreased significantly after grafting (0.94 ± 0.02 to 0.87 ± 0.01, control vs. grafting). Compared with controls, after 22 weeks of grafting, a higher percentage of follicles had initiated growth (5.6 ± 2.4 vs. 12.5 ± 1.9), but there was still a significant number of primordial follicles/graft (75 ± 6.8). Follicle stages were similar between two groups; only primordial and one-layer follicles were seen in the xenografts. In the controls, except for one two-layer follicle, the most advanced follicle was at the one-layer stage.
Conclusion(s): Human primordial follicles survive freeze-thaw and long-term xenografting procedures and retain their capacity to initiate growth. These findings encourage future attempts for human autologous ovarian transplantation.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(99)00548-8</identifier><identifier>PMID: 10689020</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Animals ; Biological and medical sciences ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; cryopreservation ; Cryopreservation - methods ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Mice ; Mice, SCID ; Molecular and cellular biology ; Ovarian Follicle - physiology ; ovarian tissue banking ; Ovarian transplant ; ovary ; Ovary - cytology ; Ovary - transplantation ; Transplantation, Heterologous ; xenograft</subject><ispartof>Fertility and sterility, 2000-03, Vol.73 (3), p.599-603</ispartof><rights>2000 American Society for Reproductive Medicine</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-8a4bf21742b3a1321a75d83bf9bde96bc245722ccd5799456296dd2b1092707a3</citedby><cites>FETCH-LOGICAL-c503t-8a4bf21742b3a1321a75d83bf9bde96bc245722ccd5799456296dd2b1092707a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(99)00548-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3548,23928,23929,25138,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1338861$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10689020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oktay, Kutluk</creatorcontrib><creatorcontrib>Newton, Helen</creatorcontrib><creatorcontrib>Gosden, Roger G</creatorcontrib><title>Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective: To determine the long-term survival of frozen-thawed human ovarian tissue as xenografts in severe-combined-immunodeficiency (SCID) mice.
Design: Animal study.
Setting: Animal and laboratory facilities at an academic center.
Patient(s): Ovarian tissue obtained from a 27-year-old woman.
Intervention(s): Grafting of frozen-thawed ovarian tissue in SCID mice for 22 weeks.
Main Outcome Measure(s): Follicle counts and growth by morphology and PCNA staining in frozen-thawed grafts and fresh controls.
Result(s): All three grafts were recovered intact after 22 weeks. Their stroma was devoid of necrotic cells and contained healthy follicles. The ratio of primordial-total follicles decreased significantly after grafting (0.94 ± 0.02 to 0.87 ± 0.01, control vs. grafting). Compared with controls, after 22 weeks of grafting, a higher percentage of follicles had initiated growth (5.6 ± 2.4 vs. 12.5 ± 1.9), but there was still a significant number of primordial follicles/graft (75 ± 6.8). Follicle stages were similar between two groups; only primordial and one-layer follicles were seen in the xenografts. In the controls, except for one two-layer follicle, the most advanced follicle was at the one-layer stage.
Conclusion(s): Human primordial follicles survive freeze-thaw and long-term xenografting procedures and retain their capacity to initiate growth. These findings encourage future attempts for human autologous ovarian transplantation.</description><subject>Adult</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Molecular and cellular biology</subject><subject>Ovarian Follicle - physiology</subject><subject>ovarian tissue banking</subject><subject>Ovarian transplant</subject><subject>ovary</subject><subject>Ovary - cytology</subject><subject>Ovary - transplantation</subject><subject>Transplantation, Heterologous</subject><subject>xenograft</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAQgC0EokvhJ4ByQAgOAT_i16lCy6OVKnFoOVuOPWGNknixnUX993WbFfTW00gz37w-hF4T_JFgIj5dYUx4i6mi77X-gDHvVKueoA3hXLRccPYUbf4hJ-hFzr8xxoJI-hydECyUxhRv0O462TnvRzsXW0Kcmzg0Lt3EfYIM6QC-2S2TremDTaHGEnJeoKnVZSy5CXMzxHEMboTmV4p_y66mQgnrrFq92l58aabg4CV6Ntgxw6tjPEU_v3293p63lz--X2w_X7aOY1ZaZbt-oER2tGeWMEqs5F6xftC9By16RzsuKXXOc6l1xwXVwnvaE6ypxNKyU_RunbtP8c8CuZgpZAdj_RDiko3EmjFJWAX5CroUc04wmH0Kk003hmBzp9jcKzZ3_ozW5l6xUbXvzXHB0k_gH3StTivw9gjY7Ow4VMEu5P8cY0oJUrGzFYNq4xAgmewCzA58SOCK8TE8csktv1iY3w</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Oktay, Kutluk</creator><creator>Newton, Helen</creator><creator>Gosden, Roger G</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice</title><author>Oktay, Kutluk ; Newton, Helen ; Gosden, Roger G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-8a4bf21742b3a1321a75d83bf9bde96bc245722ccd5799456296dd2b1092707a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Molecular and cellular biology</topic><topic>Ovarian Follicle - physiology</topic><topic>ovarian tissue banking</topic><topic>Ovarian transplant</topic><topic>ovary</topic><topic>Ovary - cytology</topic><topic>Ovary - transplantation</topic><topic>Transplantation, Heterologous</topic><topic>xenograft</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oktay, Kutluk</creatorcontrib><creatorcontrib>Newton, Helen</creatorcontrib><creatorcontrib>Gosden, Roger G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oktay, Kutluk</au><au>Newton, Helen</au><au>Gosden, Roger G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>73</volume><issue>3</issue><spage>599</spage><epage>603</epage><pages>599-603</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective: To determine the long-term survival of frozen-thawed human ovarian tissue as xenografts in severe-combined-immunodeficiency (SCID) mice.
Design: Animal study.
Setting: Animal and laboratory facilities at an academic center.
Patient(s): Ovarian tissue obtained from a 27-year-old woman.
Intervention(s): Grafting of frozen-thawed ovarian tissue in SCID mice for 22 weeks.
Main Outcome Measure(s): Follicle counts and growth by morphology and PCNA staining in frozen-thawed grafts and fresh controls.
Result(s): All three grafts were recovered intact after 22 weeks. Their stroma was devoid of necrotic cells and contained healthy follicles. The ratio of primordial-total follicles decreased significantly after grafting (0.94 ± 0.02 to 0.87 ± 0.01, control vs. grafting). Compared with controls, after 22 weeks of grafting, a higher percentage of follicles had initiated growth (5.6 ± 2.4 vs. 12.5 ± 1.9), but there was still a significant number of primordial follicles/graft (75 ± 6.8). Follicle stages were similar between two groups; only primordial and one-layer follicles were seen in the xenografts. In the controls, except for one two-layer follicle, the most advanced follicle was at the one-layer stage.
Conclusion(s): Human primordial follicles survive freeze-thaw and long-term xenografting procedures and retain their capacity to initiate growth. These findings encourage future attempts for human autologous ovarian transplantation.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10689020</pmid><doi>10.1016/S0015-0282(99)00548-8</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Animals Biological and medical sciences Cell differentiation, maturation, development, hematopoiesis Cell physiology cryopreservation Cryopreservation - methods Female Fundamental and applied biological sciences. Psychology Humans Mice Mice, SCID Molecular and cellular biology Ovarian Follicle - physiology ovarian tissue banking Ovarian transplant ovary Ovary - cytology Ovary - transplantation Transplantation, Heterologous xenograft |
| title | Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice |
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