Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene
Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for the identification of goose and mule duck foie gras. Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragment...
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Veröffentlicht in: | Journal of agricultural and food chemistry 2001-06, Vol.49 (6), p.2717-2721 |
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container_title | Journal of agricultural and food chemistry |
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creator | Rodríguez, Miguel A García, Teresa González, Isabel Asensio, Luis Fernández, Alicia Lobo, Esther Hernández, Pablo E Martín, Rosario |
description | Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for the identification of goose and mule duck foie gras. Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragments of the 5S rDNA in each species. The different sizes of the species-specific amplicons, separated by agarose gel electrophoresis, allowed clear identification of goose and mule duck foie gras samples. This genetic marker can be useful for detecting fraudulent substitution of the duck liver for the more expensive goose liver. Keywords: Species identification; Anser anser; Anas platyrhynchos x Cairina moschata; 5S rDNA; foie gras |
doi_str_mv | 10.1021/jf0014765 |
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Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragments of the 5S rDNA in each species. The different sizes of the species-specific amplicons, separated by agarose gel electrophoresis, allowed clear identification of goose and mule duck foie gras samples. This genetic marker can be useful for detecting fraudulent substitution of the duck liver for the more expensive goose liver. Keywords: Species identification; Anser anser; Anas platyrhynchos x Cairina moschata; 5S rDNA; foie gras</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf0014765</identifier><identifier>PMID: 11409956</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Anas platyrhynchos ; Animals ; Anser anser ; Base Sequence ; Biological and medical sciences ; Cairina moschata ; DNA, Ribosomal - genetics ; Ducks - classification ; Ducks - genetics ; Fatty Liver ; Food industries ; Fundamental and applied biological sciences. Psychology ; Geese - classification ; Geese - genetics ; Gene Amplification ; Meat and meat product industries ; Molecular Sequence Data ; Polymerase Chain Reaction - methods ; Poultry Products ; RNA, Ribosomal, 5S - genetics</subject><ispartof>Journal of agricultural and food chemistry, 2001-06, Vol.49 (6), p.2717-2721</ispartof><rights>Copyright © 2001 American Chemical Society</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a409t-f0e59334cb422ee92f708c02557c8d105e8bc7262872fa8d4799ec1b57bec2cd3</citedby><cites>FETCH-LOGICAL-a409t-f0e59334cb422ee92f708c02557c8d105e8bc7262872fa8d4799ec1b57bec2cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf0014765$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf0014765$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1046304$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11409956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodríguez, Miguel A</creatorcontrib><creatorcontrib>García, Teresa</creatorcontrib><creatorcontrib>González, Isabel</creatorcontrib><creatorcontrib>Asensio, Luis</creatorcontrib><creatorcontrib>Fernández, Alicia</creatorcontrib><creatorcontrib>Lobo, Esther</creatorcontrib><creatorcontrib>Hernández, Pablo E</creatorcontrib><creatorcontrib>Martín, Rosario</creatorcontrib><title>Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for the identification of goose and mule duck foie gras. Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragments of the 5S rDNA in each species. The different sizes of the species-specific amplicons, separated by agarose gel electrophoresis, allowed clear identification of goose and mule duck foie gras samples. This genetic marker can be useful for detecting fraudulent substitution of the duck liver for the more expensive goose liver. Keywords: Species identification; Anser anser; Anas platyrhynchos x Cairina moschata; 5S rDNA; foie gras</description><subject>Anas platyrhynchos</subject><subject>Animals</subject><subject>Anser anser</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cairina moschata</subject><subject>DNA, Ribosomal - genetics</subject><subject>Ducks - classification</subject><subject>Ducks - genetics</subject><subject>Fatty Liver</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geese - classification</subject><subject>Geese - genetics</subject><subject>Gene Amplification</subject><subject>Meat and meat product industries</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Poultry Products</subject><subject>RNA, Ribosomal, 5S - genetics</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEokvhwAsgHwCxh4DtxLFzXG3bbaUC1bacLceZKN4mcWon0ualeMY67KqAhMTFI_n__M94_ih6S_Bngin5sqswJinP2LNoQRjFMSNEPI8WOIixYBk5iV55v8MYC8bxy-iEkBTnOcsW0c-rErrBVEarwdgO2QptrPWAPq06Dw6p-VyGUqKvYwPobNT3s6Y86hs1TK6eOl1bj_ZorYwznUKt9bpWg1qiC2sAbVxgi2l-Ppi-gT26sc3UQrgGtK6V6dAWlP7VfdX2zV-zDDUgdou2Z99WaAMdvI5eVKrx8OZYT6MfF-d368v4-vvmar26jlX42BBXGFieJKkuUkoBclpxLDSmjHEtSoIZiEJzmlHBaaVEmfI8B00KxgvQVJfJafTx4Ns7-zCCH2RrvIamUR3Y0UuO8wQLnvwXJGHjKWUzuDyA2lnvHVSyd6ZVbpIEyzlF-ZRiYN8dTceihfI3eYwtAO-PgPJaNZVTnTb-D8c0S3AasPiAGT_A_klW7l5mPOFM3t3cSpFisWXiUorAfzjwSnu5s6Prwo7_Md8jobW-ZQ</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Rodríguez, Miguel A</creator><creator>García, Teresa</creator><creator>González, Isabel</creator><creator>Asensio, Luis</creator><creator>Fernández, Alicia</creator><creator>Lobo, Esther</creator><creator>Hernández, Pablo E</creator><creator>Martín, Rosario</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene</title><author>Rodríguez, Miguel A ; García, Teresa ; González, Isabel ; Asensio, Luis ; Fernández, Alicia ; Lobo, Esther ; Hernández, Pablo E ; Martín, Rosario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a409t-f0e59334cb422ee92f708c02557c8d105e8bc7262872fa8d4799ec1b57bec2cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Anas platyrhynchos</topic><topic>Animals</topic><topic>Anser anser</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cairina moschata</topic><topic>DNA, Ribosomal - genetics</topic><topic>Ducks - classification</topic><topic>Ducks - genetics</topic><topic>Fatty Liver</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geese - classification</topic><topic>Geese - genetics</topic><topic>Gene Amplification</topic><topic>Meat and meat product industries</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Poultry Products</topic><topic>RNA, Ribosomal, 5S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez, Miguel A</creatorcontrib><creatorcontrib>García, Teresa</creatorcontrib><creatorcontrib>González, Isabel</creatorcontrib><creatorcontrib>Asensio, Luis</creatorcontrib><creatorcontrib>Fernández, Alicia</creatorcontrib><creatorcontrib>Lobo, Esther</creatorcontrib><creatorcontrib>Hernández, Pablo E</creatorcontrib><creatorcontrib>Martín, Rosario</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodríguez, Miguel A</au><au>García, Teresa</au><au>González, Isabel</au><au>Asensio, Luis</au><au>Fernández, Alicia</au><au>Lobo, Esther</au><au>Hernández, Pablo E</au><au>Martín, Rosario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>49</volume><issue>6</issue><spage>2717</spage><epage>2721</epage><pages>2717-2721</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for the identification of goose and mule duck foie gras. Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragments of the 5S rDNA in each species. The different sizes of the species-specific amplicons, separated by agarose gel electrophoresis, allowed clear identification of goose and mule duck foie gras samples. This genetic marker can be useful for detecting fraudulent substitution of the duck liver for the more expensive goose liver. Keywords: Species identification; Anser anser; Anas platyrhynchos x Cairina moschata; 5S rDNA; foie gras</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>11409956</pmid><doi>10.1021/jf0014765</doi><tpages>5</tpages></addata></record> |
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subjects | Anas platyrhynchos Animals Anser anser Base Sequence Biological and medical sciences Cairina moschata DNA, Ribosomal - genetics Ducks - classification Ducks - genetics Fatty Liver Food industries Fundamental and applied biological sciences. Psychology Geese - classification Geese - genetics Gene Amplification Meat and meat product industries Molecular Sequence Data Polymerase Chain Reaction - methods Poultry Products RNA, Ribosomal, 5S - genetics |
title | Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene |
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