Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene

Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for the identification of goose and mule duck foie gras. Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragment...

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Veröffentlicht in:Journal of agricultural and food chemistry 2001-06, Vol.49 (6), p.2717-2721
Hauptverfasser: Rodríguez, Miguel A, García, Teresa, González, Isabel, Asensio, Luis, Fernández, Alicia, Lobo, Esther, Hernández, Pablo E, Martín, Rosario
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container_end_page 2721
container_issue 6
container_start_page 2717
container_title Journal of agricultural and food chemistry
container_volume 49
creator Rodríguez, Miguel A
García, Teresa
González, Isabel
Asensio, Luis
Fernández, Alicia
Lobo, Esther
Hernández, Pablo E
Martín, Rosario
description Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for the identification of goose and mule duck foie gras. Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragments of the 5S rDNA in each species. The different sizes of the species-specific amplicons, separated by agarose gel electrophoresis, allowed clear identification of goose and mule duck foie gras samples. This genetic marker can be useful for detecting fraudulent substitution of the duck liver for the more expensive goose liver. Keywords: Species identification; Anser anser; Anas platyrhynchos x Cairina moschata; 5S rDNA; foie gras
doi_str_mv 10.1021/jf0014765
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Agric. Food Chem</addtitle><description>Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for the identification of goose and mule duck foie gras. Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragments of the 5S rDNA in each species. The different sizes of the species-specific amplicons, separated by agarose gel electrophoresis, allowed clear identification of goose and mule duck foie gras samples. This genetic marker can be useful for detecting fraudulent substitution of the duck liver for the more expensive goose liver. 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Psychology</subject><subject>Geese - classification</subject><subject>Geese - genetics</subject><subject>Gene Amplification</subject><subject>Meat and meat product industries</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Poultry Products</subject><subject>RNA, Ribosomal, 5S - genetics</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEokvhwAsgHwCxh4DtxLFzXG3bbaUC1bacLceZKN4mcWon0ualeMY67KqAhMTFI_n__M94_ih6S_Bngin5sqswJinP2LNoQRjFMSNEPI8WOIixYBk5iV55v8MYC8bxy-iEkBTnOcsW0c-rErrBVEarwdgO2QptrPWAPq06Dw6p-VyGUqKvYwPobNT3s6Y86hs1TK6eOl1bj_ZorYwznUKt9bpWg1qiC2sAbVxgi2l-Ppi-gT26sc3UQrgGtK6V6dAWlP7VfdX2zV-zDDUgdou2Z99WaAMdvI5eVKrx8OZYT6MfF-d368v4-vvmar26jlX42BBXGFieJKkuUkoBclpxLDSmjHEtSoIZiEJzmlHBaaVEmfI8B00KxgvQVJfJafTx4Ns7-zCCH2RrvIamUR3Y0UuO8wQLnvwXJGHjKWUzuDyA2lnvHVSyd6ZVbpIEyzlF-ZRiYN8dTceihfI3eYwtAO-PgPJaNZVTnTb-D8c0S3AasPiAGT_A_klW7l5mPOFM3t3cSpFisWXiUorAfzjwSnu5s6Prwo7_Md8jobW-ZQ</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Rodríguez, Miguel A</creator><creator>García, Teresa</creator><creator>González, Isabel</creator><creator>Asensio, Luis</creator><creator>Fernández, Alicia</creator><creator>Lobo, Esther</creator><creator>Hernández, Pablo E</creator><creator>Martín, Rosario</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene</title><author>Rodríguez, Miguel A ; García, Teresa ; González, Isabel ; Asensio, Luis ; Fernández, Alicia ; Lobo, Esther ; Hernández, Pablo E ; Martín, Rosario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a409t-f0e59334cb422ee92f708c02557c8d105e8bc7262872fa8d4799ec1b57bec2cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Anas platyrhynchos</topic><topic>Animals</topic><topic>Anser anser</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cairina moschata</topic><topic>DNA, Ribosomal - genetics</topic><topic>Ducks - classification</topic><topic>Ducks - genetics</topic><topic>Fatty Liver</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geese - classification</topic><topic>Geese - genetics</topic><topic>Gene Amplification</topic><topic>Meat and meat product industries</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Poultry Products</topic><topic>RNA, Ribosomal, 5S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez, Miguel A</creatorcontrib><creatorcontrib>García, Teresa</creatorcontrib><creatorcontrib>González, Isabel</creatorcontrib><creatorcontrib>Asensio, Luis</creatorcontrib><creatorcontrib>Fernández, Alicia</creatorcontrib><creatorcontrib>Lobo, Esther</creatorcontrib><creatorcontrib>Hernández, Pablo E</creatorcontrib><creatorcontrib>Martín, Rosario</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodríguez, Miguel A</au><au>García, Teresa</au><au>González, Isabel</au><au>Asensio, Luis</au><au>Fernández, Alicia</au><au>Lobo, Esther</au><au>Hernández, Pablo E</au><au>Martín, Rosario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. 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source ACS Publications; MEDLINE
subjects Anas platyrhynchos
Animals
Anser anser
Base Sequence
Biological and medical sciences
Cairina moschata
DNA, Ribosomal - genetics
Ducks - classification
Ducks - genetics
Fatty Liver
Food industries
Fundamental and applied biological sciences. Psychology
Geese - classification
Geese - genetics
Gene Amplification
Meat and meat product industries
Molecular Sequence Data
Polymerase Chain Reaction - methods
Poultry Products
RNA, Ribosomal, 5S - genetics
title Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene
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