HSP16.6 is involved in the development of thermotolerance and thylakoid stability in the unicellular cyanobacterium, Synechocystis sp. PCC 6803
The low molecular weight (LMW) heat shock protein (HSP), HSP16.6, in the unicellular cyanobacterium, Synechocystis sp. PCC 6803, protects cells from elevated temperatures. A 95% reduction in the survival of mutant cells with an inactivated hsp16.6 was observed after exposure for 1 h at 47 degrees C....
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| Veröffentlicht in: | Current microbiology 2000-04, Vol.40 (4), p.283-287 |
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| creator | LEE, S OWEN, H. A PROCHASKA, D. J BARNUM, S. R |
| description | The low molecular weight (LMW) heat shock protein (HSP), HSP16.6, in the unicellular cyanobacterium, Synechocystis sp. PCC 6803, protects cells from elevated temperatures. A 95% reduction in the survival of mutant cells with an inactivated hsp16.6 was observed after exposure for 1 h at 47 degrees C. Wild-type cell survival was reduced to only 41%. HSP16.6 is also involved in the development of thermotolerance. After a sublethal heat shock at 43 degrees C for 1 h and subsequent challenge exposure at 49 degrees C for 40 min, mutant cells did not survive, while 64% of wild-type cells survived. Ultrastructural changes in the integrity of thylakoid membranes of heat-shocked mutant cells also are discussed. These results demonstrate an important protective role for HSP16.6 in the protection of cells and, in particular, thylakoid membrane against thermal stress. |
| doi_str_mv | 10.1007/s002849910056 |
| format | Article |
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A</au><au>PROCHASKA, D. J</au><au>BARNUM, S. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HSP16.6 is involved in the development of thermotolerance and thylakoid stability in the unicellular cyanobacterium, Synechocystis sp. PCC 6803</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>2000-04-01</date><risdate>2000</risdate><volume>40</volume><issue>4</issue><spage>283</spage><epage>287</epage><pages>283-287</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>The low molecular weight (LMW) heat shock protein (HSP), HSP16.6, in the unicellular cyanobacterium, Synechocystis sp. PCC 6803, protects cells from elevated temperatures. A 95% reduction in the survival of mutant cells with an inactivated hsp16.6 was observed after exposure for 1 h at 47 degrees C. Wild-type cell survival was reduced to only 41%. HSP16.6 is also involved in the development of thermotolerance. After a sublethal heat shock at 43 degrees C for 1 h and subsequent challenge exposure at 49 degrees C for 40 min, mutant cells did not survive, while 64% of wild-type cells survived. Ultrastructural changes in the integrity of thylakoid membranes of heat-shocked mutant cells also are discussed. These results demonstrate an important protective role for HSP16.6 in the protection of cells and, in particular, thylakoid membrane against thermal stress.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>10688700</pmid><doi>10.1007/s002849910056</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 2000-04, Vol.40 (4), p.283-287 |
| issn | 0343-8651 1432-0991 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Action of physical and chemical agents on bacteria Bacteriology Biological and medical sciences Cell Membrane - metabolism Cell Membrane - ultrastructure Cyanobacteria Cyanobacteria - physiology Cyanobacteria - ultrastructure Fundamental and applied biological sciences. Psychology Heat-Shock Proteins - genetics Heat-Shock Proteins - metabolism High temperature Microbiology Microscopy, Electron Mutation Synechocystis Temperature Thermal stress Time Factors |
| title | HSP16.6 is involved in the development of thermotolerance and thylakoid stability in the unicellular cyanobacterium, Synechocystis sp. PCC 6803 |
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