Human milk fractions inhibit the adherence of diffusely adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) to HeLa cells
Binding to a specific receptor is an essential step for most enteropathogens to initiate an intestinal infection. We analyzed the inhibitory effect of human milk and its protein components on adhesion of two diarrheagenic Escherichia coli strains, diffusely adherent E. coli (DAEC) and enteroaggregat...
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| Veröffentlicht in: | FEMS microbiology letters 2000-03, Vol.184 (1), p.91-94 |
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| creator | Araujo, A.N. de Giugliano, L.G |
| description | Binding to a specific receptor is an essential step for most enteropathogens to initiate an intestinal infection. We analyzed the inhibitory effect of human milk and its protein components on adhesion of two diarrheagenic Escherichia coli strains, diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC), to HeLa cells. Defatted milk, whey proteins, immunoglobulin and non-immunoglobulin fractions, in concentrations lower than usually found in whole milk, inhibited both DAEC and EAEC adhesion, indicating that human milk components may contribute to the defense of the infants against enteropathogens. |
| doi_str_mv | 10.1111/j.1574-6968.2000.tb08996.x |
| format | Article |
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We analyzed the inhibitory effect of human milk and its protein components on adhesion of two diarrheagenic Escherichia coli strains, diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC), to HeLa cells. Defatted milk, whey proteins, immunoglobulin and non-immunoglobulin fractions, in concentrations lower than usually found in whole milk, inhibited both DAEC and EAEC adhesion, indicating that human milk components may contribute to the defense of the infants against enteropathogens.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2000.tb08996.x</identifier><identifier>PMID: 10689172</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adherence inhibition ; Adhesion ; Baby foods ; Bacteria ; Bacterial Adhesion - drug effects ; Bacteriology ; Biological and medical sciences ; Breast milk ; Chemical Fractionation ; Diarrhea ; Diffusely adherent Escherichia coli ; E coli ; Electrophoresis, Polyacrylamide Gel ; Enteroaggregative Escherichia coli ; enteropathogens ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - physiology ; Fundamental and applied biological sciences. Psychology ; HeLa Cells ; Human milk ; human milk proteins ; Humans ; Immunoglobulin Heavy Chains - pharmacology ; Immunoglobulins ; Infants ; Intestine ; Lactalbumin - pharmacology ; Lactoferrin - pharmacology ; Microbiology ; Milk ; Milk Proteins - pharmacology ; Milk, Human - chemistry ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Proteins ; Serum Albumin - pharmacology ; Whey ; Whey protein ; Whey Proteins ; whole milk</subject><ispartof>FEMS microbiology letters, 2000-03, Vol.184 (1), p.91-94</ispartof><rights>2000 Federation of European Microbiological Societies 2000</rights><rights>2000 INIST-CNRS</rights><rights>2000 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4741-67b4dc66fcbe51d9d31813c316def18ed64c037b94be8a008218bd76f6c2613e3</citedby><cites>FETCH-LOGICAL-c4741-67b4dc66fcbe51d9d31813c316def18ed64c037b94be8a008218bd76f6c2613e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2000.tb08996.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2000.tb08996.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1307700$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10689172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Araujo, A.N. de</creatorcontrib><creatorcontrib>Giugliano, L.G</creatorcontrib><title>Human milk fractions inhibit the adherence of diffusely adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) to HeLa cells</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Binding to a specific receptor is an essential step for most enteropathogens to initiate an intestinal infection. We analyzed the inhibitory effect of human milk and its protein components on adhesion of two diarrheagenic Escherichia coli strains, diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC), to HeLa cells. Defatted milk, whey proteins, immunoglobulin and non-immunoglobulin fractions, in concentrations lower than usually found in whole milk, inhibited both DAEC and EAEC adhesion, indicating that human milk components may contribute to the defense of the infants against enteropathogens.</description><subject>Adherence inhibition</subject><subject>Adhesion</subject><subject>Baby foods</subject><subject>Bacteria</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Breast milk</subject><subject>Chemical Fractionation</subject><subject>Diarrhea</subject><subject>Diffusely adherent Escherichia coli</subject><subject>E coli</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enteroaggregative Escherichia coli</subject><subject>enteropathogens</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells</subject><subject>Human milk</subject><subject>human milk proteins</subject><subject>Humans</subject><subject>Immunoglobulin Heavy Chains - pharmacology</subject><subject>Immunoglobulins</subject><subject>Infants</subject><subject>Intestine</subject><subject>Lactalbumin - pharmacology</subject><subject>Lactoferrin - pharmacology</subject><subject>Microbiology</subject><subject>Milk</subject><subject>Milk Proteins - pharmacology</subject><subject>Milk, Human - chemistry</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Proteins</subject><subject>Serum Albumin - pharmacology</subject><subject>Whey</subject><subject>Whey protein</subject><subject>Whey Proteins</subject><subject>whole milk</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkcGO0zAURSMEYsrAL4AFCMEiwU5S20FiMSodilTEYpi15TgvrUsSd-wEpt_AT_NCCoMQIJGNo_hc5z6fKHrMaMLweblL2FzkMS-4TFJKadKXVBYFT65vRbOfW7ejGc2EjBktxEl0L4QdonlK-d3ohFEuCybSWfR1NbS6I61tPpHaa9Nb1wViu60tbU_6LRBdbcFDZ4C4mlS2rocAzeHH554sg8E3a7ZWE-MaS56_OVsuXhDdVQT3wTu92XjY6N5-BrJMjtDyO9Q7soI1BqFpwv3oTq2bAA-O62l0eb78uFjF6w9v3y3O1rHJRc5iLsq8MpzXpoQ5q4oqY5JlJmO8gppJqHhucPKyyEuQmlKZMllWgtfcpJxlkJ1Gz6Zz995dDRB61dowNtAduCEoQYtUZJQh-OQ3cOcG32E3lWYML5Hjf5B6NVHGuxA81Grvbav9QTGqRmFqp0YrarSiRmHqKExdY3j-jzBlXF2MFtVoUWES06lUEnMPj9WGsoXql9RkFoGnR0AHoxt02xkbbriMCkEpYq8n7Itt4PAfzdX5-3XBbvq7Yf-XdPznuR9NuVo7pTceq11epDgvYgXnaZ59AzCi2ik</recordid><startdate>200003</startdate><enddate>200003</enddate><creator>Araujo, A.N. de</creator><creator>Giugliano, L.G</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200003</creationdate><title>Human milk fractions inhibit the adherence of diffusely adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) to HeLa cells</title><author>Araujo, A.N. de ; Giugliano, L.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4741-67b4dc66fcbe51d9d31813c316def18ed64c037b94be8a008218bd76f6c2613e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adherence inhibition</topic><topic>Adhesion</topic><topic>Baby foods</topic><topic>Bacteria</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Breast milk</topic><topic>Chemical Fractionation</topic><topic>Diarrhea</topic><topic>Diffusely adherent Escherichia coli</topic><topic>E coli</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enteroaggregative Escherichia coli</topic><topic>enteropathogens</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa Cells</topic><topic>Human milk</topic><topic>human milk proteins</topic><topic>Humans</topic><topic>Immunoglobulin Heavy Chains - pharmacology</topic><topic>Immunoglobulins</topic><topic>Infants</topic><topic>Intestine</topic><topic>Lactalbumin - pharmacology</topic><topic>Lactoferrin - pharmacology</topic><topic>Microbiology</topic><topic>Milk</topic><topic>Milk Proteins - pharmacology</topic><topic>Milk, Human - chemistry</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Proteins</topic><topic>Serum Albumin - pharmacology</topic><topic>Whey</topic><topic>Whey protein</topic><topic>Whey Proteins</topic><topic>whole milk</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Araujo, A.N. de</creatorcontrib><creatorcontrib>Giugliano, L.G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Araujo, A.N. de</au><au>Giugliano, L.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human milk fractions inhibit the adherence of diffusely adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) to HeLa cells</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2000-03</date><risdate>2000</risdate><volume>184</volume><issue>1</issue><spage>91</spage><epage>94</epage><pages>91-94</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Binding to a specific receptor is an essential step for most enteropathogens to initiate an intestinal infection. We analyzed the inhibitory effect of human milk and its protein components on adhesion of two diarrheagenic Escherichia coli strains, diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC), to HeLa cells. Defatted milk, whey proteins, immunoglobulin and non-immunoglobulin fractions, in concentrations lower than usually found in whole milk, inhibited both DAEC and EAEC adhesion, indicating that human milk components may contribute to the defense of the infants against enteropathogens.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10689172</pmid><doi>10.1111/j.1574-6968.2000.tb08996.x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | FEMS microbiology letters, 2000-03, Vol.184 (1), p.91-94 |
| issn | 0378-1097 1574-6968 |
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| source | MEDLINE; Wiley Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adherence inhibition Adhesion Baby foods Bacteria Bacterial Adhesion - drug effects Bacteriology Biological and medical sciences Breast milk Chemical Fractionation Diarrhea Diffusely adherent Escherichia coli E coli Electrophoresis, Polyacrylamide Gel Enteroaggregative Escherichia coli enteropathogens Escherichia coli Escherichia coli - drug effects Escherichia coli - physiology Fundamental and applied biological sciences. Psychology HeLa Cells Human milk human milk proteins Humans Immunoglobulin Heavy Chains - pharmacology Immunoglobulins Infants Intestine Lactalbumin - pharmacology Lactoferrin - pharmacology Microbiology Milk Milk Proteins - pharmacology Milk, Human - chemistry Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Proteins Serum Albumin - pharmacology Whey Whey protein Whey Proteins whole milk |
| title | Human milk fractions inhibit the adherence of diffusely adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) to HeLa cells |
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