Loss of heterozygosity in P53, BRCA1, and estrogen receptor genes and correlation to expression of p53 protein in ovarian epithelial tumors of different cell types and biological behavior

Loss of heterozygosity (LOH) of tumor suppressor genes (TSGs) in ovarian epithelial tumors of differing cell types and biological behavior has not been thoroughly investigated. Moreover, there have been conflicting reports correlating LOH of the p53 gene to overexpression of p53 protein. This study...

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Veröffentlicht in:Human pathology 2000-02, Vol.31 (2), p.233-238
Hauptverfasser: Otis, Christopher N., Krebs, Patricia A., Quezado, Martha M., Albuquerque, Ana, Bryant, Bonita, Juan, Xavier San, Kleiner, David, Sobel, Mark E., Merino, Maria J.
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container_end_page 238
container_issue 2
container_start_page 233
container_title Human pathology
container_volume 31
creator Otis, Christopher N.
Krebs, Patricia A.
Quezado, Martha M.
Albuquerque, Ana
Bryant, Bonita
Juan, Xavier San
Kleiner, David
Sobel, Mark E.
Merino, Maria J.
description Loss of heterozygosity (LOH) of tumor suppressor genes (TSGs) in ovarian epithelial tumors of differing cell types and biological behavior has not been thoroughly investigated. Moreover, there have been conflicting reports correlating LOH of the p53 gene to overexpression of p53 protein. This study evaluated 34 formalin-fixed, paraffin-embedded ovarian epithelial tumors for LOH by polymerase chain reaction (PCR) for the following microsatellite markers: TP53(17p13.1/ p53 gene), D17S579(17q/BRCA1 gene), and ESR (6q24–27/estrogen receptor gene). LOH of the TP53 marker was detected in 4 (44%) of 9 informative serous cystadenocarcinomas (SCa) but in 0 of 4 informative clear cell carcinomas (CCa) and 0 of 5 informative serous tumors of low malignant potential (SLMP). LOH of the BRCA1 marker was detected in 5 (83%) of 6 informative SCa, but in 1 (13%) of 8 informative CCa and 1 (14%) of 7 informative SLMP. LOH of the ESR marker was detected in 4 (50%) of 8 informative SCa, but in 0 of 4 informative CCa and 1 (16%) of 6 informative SLMP. p53 protein overexpression was present in 8 of 12 SCa but did not correlate to TP53 LOH. LOH for TP53, D17S579/BRCA1, and ESR is common in ovarian SCa, and is observed in primary tumors as well as metastases. In contrast, these genetic alterations are less common in CCa and in the biologically less aggressive SLMP tumors. These data suggest different mechanisms of oncogenesis in ovarian epithelial tumors of different cell types and biological behavior.
doi_str_mv 10.1016/S0046-8177(00)80225-7
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LOH for TP53, D17S579/BRCA1, and ESR is common in ovarian SCa, and is observed in primary tumors as well as metastases. In contrast, these genetic alterations are less common in CCa and in the biologically less aggressive SLMP tumors. These data suggest different mechanisms of oncogenesis in ovarian epithelial tumors of different cell types and biological behavior.</description><subject>Adenocarcinoma, Clear Cell - genetics</subject><subject>Biological and medical sciences</subject><subject>BRCA1</subject><subject>BRCA1 Protein - genetics</subject><subject>Cystadenocarcinoma, Serous - genetics</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gene Expression</subject><subject>Genes, p53</subject><subject>Gynecology. Andrology. 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Moreover, there have been conflicting reports correlating LOH of the p53 gene to overexpression of p53 protein. This study evaluated 34 formalin-fixed, paraffin-embedded ovarian epithelial tumors for LOH by polymerase chain reaction (PCR) for the following microsatellite markers: TP53(17p13.1/ p53 gene), D17S579(17q/BRCA1 gene), and ESR (6q24–27/estrogen receptor gene). LOH of the TP53 marker was detected in 4 (44%) of 9 informative serous cystadenocarcinomas (SCa) but in 0 of 4 informative clear cell carcinomas (CCa) and 0 of 5 informative serous tumors of low malignant potential (SLMP). LOH of the BRCA1 marker was detected in 5 (83%) of 6 informative SCa, but in 1 (13%) of 8 informative CCa and 1 (14%) of 7 informative SLMP. LOH of the ESR marker was detected in 4 (50%) of 8 informative SCa, but in 0 of 4 informative CCa and 1 (16%) of 6 informative SLMP. p53 protein overexpression was present in 8 of 12 SCa but did not correlate to TP53 LOH. LOH for TP53, D17S579/BRCA1, and ESR is common in ovarian SCa, and is observed in primary tumors as well as metastases. In contrast, these genetic alterations are less common in CCa and in the biologically less aggressive SLMP tumors. These data suggest different mechanisms of oncogenesis in ovarian epithelial tumors of different cell types and biological behavior.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10685639</pmid><doi>10.1016/S0046-8177(00)80225-7</doi><tpages>6</tpages></addata></record>
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subjects Adenocarcinoma, Clear Cell - genetics
Biological and medical sciences
BRCA1
BRCA1 Protein - genetics
Cystadenocarcinoma, Serous - genetics
Female
Female genital diseases
Gene Expression
Genes, p53
Gynecology. Andrology. Obstetrics
Humans
LOH
Loss of Heterozygosity
Medical sciences
Microsatellite Repeats
ovarian carcinoma
Ovarian Neoplasms - genetics
p53
Polymerase Chain Reaction
Receptors, Estrogen - genetics
Tumors
title Loss of heterozygosity in P53, BRCA1, and estrogen receptor genes and correlation to expression of p53 protein in ovarian epithelial tumors of different cell types and biological behavior
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