Quantitative Microscopy of Fluorescent Adenovirus Entry

Fluorescence imaging of cells is a powerful tool for exploring the dynamics of organelles, proteins, and viruses. Fluorescent adenoviruses are a model system for cargo transport from the cell surface to the nucleus. Here, we describe a procedure to quantitate adenovirus-associated fluorescence in di...

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Veröffentlicht in:	Journal of structural biology 2000-02, Vol.129 (1), p.57-68
Hauptverfasser:	Nakano, M.Y., Greber, U.F.
Format:	Artikel
Sprache:	eng
Schlagworte:	adenovirus transport Adenoviruses, Human - genetics Adenoviruses, Human - physiology Adenoviruses, Human - ultrastructure Cell Nucleus - virology Cells, Cultured cytoplasm deblurring Defective Viruses - genetics Defective Viruses - physiology Defective Viruses - ultrastructure Fluorescent Dyes fluorescent virus Fourier Analysis HeLa Cells - ultrastructure HeLa Cells - virology Humans image processing Image Processing, Computer-Assisted Microscopy, Fluorescence nucleus plasma membrane quantitative microscopy Subtraction Technique thresholding Xanthenes
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