Assessment of template quality by the incorporation of an internal control into a RT-PCR for the detection of rabies and rabies-related viruses

A method is described to assess RNA template quality by the incorporation of a ribosomal RNA (rRNA) internal (in tube) control into a standard rabies and rabies-related virus specific RT-PCR. Specific virus and rRNA templates were co-amplified in a duplex reaction from RNA extracts derived from 60 i...

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Veröffentlicht in:	Journal of virological methods 2000-02, Vol.84 (2), p.107-115
Hauptverfasser:	Smith, Jemma, McElhinney, Lorraine M, Heaton, Paul R, Black, Elizabeth M, Lowings, J.Paul
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Blotting, Southern Brain - virology Cats Cattle Cell Line Cricetinae Diverse techniques Dogs Duplex RT-PCR Fundamental and applied biological sciences. Psychology Humans Lyssavirus - genetics Lyssavirus - isolation & purification Mice Microbiology Molecular and cellular biology Rabies - diagnosis Rabies virus Rabies virus - genetics Rabies virus - isolation & purification Rabies-related viruses Reference Standards Reverse Transcriptase Polymerase Chain Reaction - methods Ribosomal RNA RNA, Ribosomal - analysis RNA, Viral - analysis Sensitivity and Specificity Techniques used in virology Templates, Genetic Virology
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