Processing cell-seeded polyester scaffolds for histology
Biodegradable 3‐dimensional scaffolds of various morphologies are currently being developed for tissue engineering. Poly(lactide‐co‐glycolide)s (PLGAs) of various lactide to glycolide ratios are frequently used for such applications. Tissue engineering involves an in vitro stage during which cells a...
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| Veröffentlicht in: | Journal of biomedical materials research 2000-05, Vol.50 (2), p.276-279 |
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| creator | Holy, Chantal E. Yakubovich, Raisa |
| description | Biodegradable 3‐dimensional scaffolds of various morphologies are currently being developed for tissue engineering. Poly(lactide‐co‐glycolide)s (PLGAs) of various lactide to glycolide ratios are frequently used for such applications. Tissue engineering involves an in vitro stage during which cells are seeded onto scaffolds and allowed to settle and/or grow for various time periods. To assess cell distribution and/or tissue formation throughout the scaffolds during this in vitro stage, techniques such as confocal microscopy and magnetic resonance imaging have been applied. However, such cultured scaffolds have been refractory to histological evaluation because of numerous technical difficulties. We describe a method to prepare histological sections of cell cultured PLGA scaffolds for tissue engineering. The technique involves in situ labeling of cultured scaffolds, infiltration of the scaffolds with a 10% poly(vinyl alcohol) solution under a low vacuum, and cryosectioning of samples onto acid‐treated glass coverslips. Sections obtained with this technique show cell distribution and cell–tissue morphology on the pore wall structures of entire centimeter‐thick scaffolds. This rapid and easy technique allows for fast evaluation of tissues grown on biodegradable scaffolds. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 50, 276–279, 2000. |
| doi_str_mv | 10.1002/(SICI)1097-4636(200005)50:2<276::AID-JBM23>3.0.CO;2-2 |
| format | Article |
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Poly(lactide‐co‐glycolide)s (PLGAs) of various lactide to glycolide ratios are frequently used for such applications. Tissue engineering involves an in vitro stage during which cells are seeded onto scaffolds and allowed to settle and/or grow for various time periods. To assess cell distribution and/or tissue formation throughout the scaffolds during this in vitro stage, techniques such as confocal microscopy and magnetic resonance imaging have been applied. However, such cultured scaffolds have been refractory to histological evaluation because of numerous technical difficulties. We describe a method to prepare histological sections of cell cultured PLGA scaffolds for tissue engineering. The technique involves in situ labeling of cultured scaffolds, infiltration of the scaffolds with a 10% poly(vinyl alcohol) solution under a low vacuum, and cryosectioning of samples onto acid‐treated glass coverslips. Sections obtained with this technique show cell distribution and cell–tissue morphology on the pore wall structures of entire centimeter‐thick scaffolds. This rapid and easy technique allows for fast evaluation of tissues grown on biodegradable scaffolds. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 50, 276–279, 2000.</description><identifier>ISSN: 0021-9304</identifier><identifier>EISSN: 1097-4636</identifier><identifier>DOI: 10.1002/(SICI)1097-4636(200005)50:2<276::AID-JBM23>3.0.CO;2-2</identifier><identifier>PMID: 10679693</identifier><identifier>CODEN: JBMRBG</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Animals ; Biodegradation ; Biological and medical sciences ; Biomedical engineering ; Bone and Bones - pathology ; Bone Substitutes ; bone tissue engineering ; Cell culture ; Cells ; Growth kinetics ; Histological Techniques ; histology ; Humans ; Medical sciences ; Microscopic examination ; Morphology ; Nuclear magnetic resonance spectroscopy ; polyester ; Polyesters ; polylactide-co-glycolide ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; scaffolds ; Technology. Biomaterials. 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Biomed. Mater. Res</addtitle><description>Biodegradable 3‐dimensional scaffolds of various morphologies are currently being developed for tissue engineering. Poly(lactide‐co‐glycolide)s (PLGAs) of various lactide to glycolide ratios are frequently used for such applications. Tissue engineering involves an in vitro stage during which cells are seeded onto scaffolds and allowed to settle and/or grow for various time periods. To assess cell distribution and/or tissue formation throughout the scaffolds during this in vitro stage, techniques such as confocal microscopy and magnetic resonance imaging have been applied. However, such cultured scaffolds have been refractory to histological evaluation because of numerous technical difficulties. We describe a method to prepare histological sections of cell cultured PLGA scaffolds for tissue engineering. The technique involves in situ labeling of cultured scaffolds, infiltration of the scaffolds with a 10% poly(vinyl alcohol) solution under a low vacuum, and cryosectioning of samples onto acid‐treated glass coverslips. Sections obtained with this technique show cell distribution and cell–tissue morphology on the pore wall structures of entire centimeter‐thick scaffolds. This rapid and easy technique allows for fast evaluation of tissues grown on biodegradable scaffolds. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 50, 276–279, 2000.</description><subject>Animals</subject><subject>Biodegradation</subject><subject>Biological and medical sciences</subject><subject>Biomedical engineering</subject><subject>Bone and Bones - pathology</subject><subject>Bone Substitutes</subject><subject>bone tissue engineering</subject><subject>Cell culture</subject><subject>Cells</subject><subject>Growth kinetics</subject><subject>Histological Techniques</subject><subject>histology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microscopic examination</subject><subject>Morphology</subject><subject>Nuclear magnetic resonance spectroscopy</subject><subject>polyester</subject><subject>Polyesters</subject><subject>polylactide-co-glycolide</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>scaffolds</subject><subject>Technology. Biomaterials. Equipments. Material. 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Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>scaffolds</topic><topic>Technology. Biomaterials. Equipments. Material. Instrumentation</topic><topic>Tissue</topic><toplevel>online_resources</toplevel><creatorcontrib>Holy, Chantal E.</creatorcontrib><creatorcontrib>Yakubovich, Raisa</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holy, Chantal E.</au><au>Yakubovich, Raisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Processing cell-seeded polyester scaffolds for histology</atitle><jtitle>Journal of biomedical materials research</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2000-05</date><risdate>2000</risdate><volume>50</volume><issue>2</issue><spage>276</spage><epage>279</epage><pages>276-279</pages><issn>0021-9304</issn><eissn>1097-4636</eissn><coden>JBMRBG</coden><abstract>Biodegradable 3‐dimensional scaffolds of various morphologies are currently being developed for tissue engineering. Poly(lactide‐co‐glycolide)s (PLGAs) of various lactide to glycolide ratios are frequently used for such applications. Tissue engineering involves an in vitro stage during which cells are seeded onto scaffolds and allowed to settle and/or grow for various time periods. To assess cell distribution and/or tissue formation throughout the scaffolds during this in vitro stage, techniques such as confocal microscopy and magnetic resonance imaging have been applied. However, such cultured scaffolds have been refractory to histological evaluation because of numerous technical difficulties. We describe a method to prepare histological sections of cell cultured PLGA scaffolds for tissue engineering. The technique involves in situ labeling of cultured scaffolds, infiltration of the scaffolds with a 10% poly(vinyl alcohol) solution under a low vacuum, and cryosectioning of samples onto acid‐treated glass coverslips. Sections obtained with this technique show cell distribution and cell–tissue morphology on the pore wall structures of entire centimeter‐thick scaffolds. This rapid and easy technique allows for fast evaluation of tissues grown on biodegradable scaffolds. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 50, 276–279, 2000.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>10679693</pmid><doi>10.1002/(SICI)1097-4636(200005)50:2<276::AID-JBM23>3.0.CO;2-2</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Biodegradation Biological and medical sciences Biomedical engineering Bone and Bones - pathology Bone Substitutes bone tissue engineering Cell culture Cells Growth kinetics Histological Techniques histology Humans Medical sciences Microscopic examination Morphology Nuclear magnetic resonance spectroscopy polyester Polyesters polylactide-co-glycolide Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) scaffolds Technology. Biomaterials. Equipments. Material. Instrumentation Tissue |
| title | Processing cell-seeded polyester scaffolds for histology |
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