Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes
The objective of the present study was to understand the role of cortisol in the cellular stress response process in fish. Specifically, our studies addressed whether cortisol exposure modified heat shock protein 90 (HSP90) mRNA expression in rainbow trout (Oncorhynchus mykiss) hepatocytes maintaine...
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| Veröffentlicht in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2001-06, Vol.129 (2-3), p.679-685 |
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| container_title | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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| creator | Sathiyaa, Ramesh Campbell, Tracey Vijayan, Mathilakath M |
| description | The objective of the present study was to understand the role of cortisol in the cellular stress response process in fish. Specifically, our studies addressed whether cortisol exposure modified heat shock protein 90 (HSP90) mRNA expression in rainbow trout (Oncorhynchus mykiss) hepatocytes maintained in primary culture. We also subjected these hepatocytes to heat shock (HS) in order to examine the role of cortisol on HS-induced HSP90 mRNA expression. A cDNA fragment of 500 bp was cloned from trout liver by reverse transcriptase- polymerase chain reaction (RT-PCR) with primers designed from the conserved regions of chinook salmon and zebrafish HSP90 cDNAs. The PCR product showed very high homology to chinook salmon (98%), zebrafish (84%) and human (77%) HSP90. Heat shock (+6°C) induced transient elevation in HSP90 mRNA in trout hepatocytes, peaking within 10-h post-HS, and remained elevated over a 24-h period. Cortisol did not modify the unstimulated expression of HSP90 mRNA, whereas the HS-induced HSP90 mRNA expression was attenuated in trout hepatocytes. Our results suggest that elevated plasma cortisol levels modulate the cellular stress response by affecting the transcription of HSP90 in fish. |
| doi_str_mv | 10.1016/S1096-4959(01)00373-6 |
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Specifically, our studies addressed whether cortisol exposure modified heat shock protein 90 (HSP90) mRNA expression in rainbow trout (Oncorhynchus mykiss) hepatocytes maintained in primary culture. We also subjected these hepatocytes to heat shock (HS) in order to examine the role of cortisol on HS-induced HSP90 mRNA expression. A cDNA fragment of 500 bp was cloned from trout liver by reverse transcriptase- polymerase chain reaction (RT-PCR) with primers designed from the conserved regions of chinook salmon and zebrafish HSP90 cDNAs. The PCR product showed very high homology to chinook salmon (98%), zebrafish (84%) and human (77%) HSP90. Heat shock (+6°C) induced transient elevation in HSP90 mRNA in trout hepatocytes, peaking within 10-h post-HS, and remained elevated over a 24-h period. Cortisol did not modify the unstimulated expression of HSP90 mRNA, whereas the HS-induced HSP90 mRNA expression was attenuated in trout hepatocytes. Our results suggest that elevated plasma cortisol levels modulate the cellular stress response by affecting the transcription of HSP90 in fish.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/S1096-4959(01)00373-6</identifier><identifier>PMID: 11399505</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Cells, Cultured ; Cortisol ; Fish ; Gene Expression Regulation - drug effects ; Glucocorticoid receptors ; Heat shock protein ; Hepatocyte primary cultures ; Hepatocytes - cytology ; Hepatocytes - drug effects ; Hepatocytes - metabolism ; HSP90 Heat-Shock Proteins - chemistry ; HSP90 Heat-Shock Proteins - genetics ; HSP90 mRNA ; Hydrocortisone - pharmacology ; Molecular Sequence Data ; Oncorhynchus mykiss ; Oncorhynchus mykiss - genetics ; Rainbow trout ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Stress ; Temperature shock ; Time Factors</subject><ispartof>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2001-06, Vol.129 (2-3), p.679-685</ispartof><rights>2001 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-5821734caaf481f1e67a1057bb2f9938ce76ce96bdfb9ea7ae118eca204763263</citedby><cites>FETCH-LOGICAL-c515t-5821734caaf481f1e67a1057bb2f9938ce76ce96bdfb9ea7ae118eca204763263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1096-4959(01)00373-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,315,781,785,793,3551,27924,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11399505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sathiyaa, Ramesh</creatorcontrib><creatorcontrib>Campbell, Tracey</creatorcontrib><creatorcontrib>Vijayan, Mathilakath M</creatorcontrib><title>Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes</title><title>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</title><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><description>The objective of the present study was to understand the role of cortisol in the cellular stress response process in fish. Specifically, our studies addressed whether cortisol exposure modified heat shock protein 90 (HSP90) mRNA expression in rainbow trout (Oncorhynchus mykiss) hepatocytes maintained in primary culture. We also subjected these hepatocytes to heat shock (HS) in order to examine the role of cortisol on HS-induced HSP90 mRNA expression. A cDNA fragment of 500 bp was cloned from trout liver by reverse transcriptase- polymerase chain reaction (RT-PCR) with primers designed from the conserved regions of chinook salmon and zebrafish HSP90 cDNAs. The PCR product showed very high homology to chinook salmon (98%), zebrafish (84%) and human (77%) HSP90. Heat shock (+6°C) induced transient elevation in HSP90 mRNA in trout hepatocytes, peaking within 10-h post-HS, and remained elevated over a 24-h period. Cortisol did not modify the unstimulated expression of HSP90 mRNA, whereas the HS-induced HSP90 mRNA expression was attenuated in trout hepatocytes. Our results suggest that elevated plasma cortisol levels modulate the cellular stress response by affecting the transcription of HSP90 in fish.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cortisol</subject><subject>Fish</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glucocorticoid receptors</subject><subject>Heat shock protein</subject><subject>Hepatocyte primary cultures</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - metabolism</subject><subject>HSP90 Heat-Shock Proteins - chemistry</subject><subject>HSP90 Heat-Shock Proteins - genetics</subject><subject>HSP90 mRNA</subject><subject>Hydrocortisone - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - genetics</subject><subject>Rainbow trout</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Stress</subject><subject>Temperature shock</subject><subject>Time Factors</subject><issn>1096-4959</issn><issn>1879-1107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0UuPFCEQB3BiNO5DP4LKyayHVqppXiezmbiuycbXuGdC09WK6R5mgTbut5fZGeNNPRAI-RVU6k_IE2AvgYF8tQZmZNMZYc4YvGCMK97Ie-QYtDINAFP36_k3OSInOX-vSAOHh-QIgBsjmDgmn1YxlZDjROc4LJMrmOnl-qNhdP78_pziz23CnEPc0LCh2xRml26pX6ay1HsaR1pSXAr9hltXor-t5Y_Ig9FNGR8f9lNyffHmy-qyufrw9t3q_KrxAkRphG5B8c47N3YaRkCpHDCh-r4djeHao5IejeyHsTfolEMAjd61rFOSt5Kfkuf7d7cp3iyYi51D9jhNboNxyVYxA5L9BwTNtWqFrvDs71BK0WljVFep2FOfYs4JR3uYjQVmd_nYu3zsbviWgb3Lx-56eXr4YulnHP5UHQKp4NkejC5a9zWFbK_XbX2Q1QXS7MTrvcA63B8Bk80-4MbjEBL6YocY_tHELwVzqEI</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Sathiyaa, Ramesh</creator><creator>Campbell, Tracey</creator><creator>Vijayan, Mathilakath M</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes</title><author>Sathiyaa, Ramesh ; Campbell, Tracey ; Vijayan, Mathilakath M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-5821734caaf481f1e67a1057bb2f9938ce76ce96bdfb9ea7ae118eca204763263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Cortisol</topic><topic>Fish</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glucocorticoid receptors</topic><topic>Heat shock protein</topic><topic>Hepatocyte primary cultures</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - metabolism</topic><topic>HSP90 Heat-Shock Proteins - chemistry</topic><topic>HSP90 Heat-Shock Proteins - genetics</topic><topic>HSP90 mRNA</topic><topic>Hydrocortisone - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - genetics</topic><topic>Rainbow trout</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Stress</topic><topic>Temperature shock</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sathiyaa, Ramesh</creatorcontrib><creatorcontrib>Campbell, Tracey</creatorcontrib><creatorcontrib>Vijayan, Mathilakath M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sathiyaa, Ramesh</au><au>Campbell, Tracey</au><au>Vijayan, Mathilakath M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes</atitle><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>129</volume><issue>2-3</issue><spage>679</spage><epage>685</epage><pages>679-685</pages><issn>1096-4959</issn><eissn>1879-1107</eissn><abstract>The objective of the present study was to understand the role of cortisol in the cellular stress response process in fish. Specifically, our studies addressed whether cortisol exposure modified heat shock protein 90 (HSP90) mRNA expression in rainbow trout (Oncorhynchus mykiss) hepatocytes maintained in primary culture. We also subjected these hepatocytes to heat shock (HS) in order to examine the role of cortisol on HS-induced HSP90 mRNA expression. A cDNA fragment of 500 bp was cloned from trout liver by reverse transcriptase- polymerase chain reaction (RT-PCR) with primers designed from the conserved regions of chinook salmon and zebrafish HSP90 cDNAs. The PCR product showed very high homology to chinook salmon (98%), zebrafish (84%) and human (77%) HSP90. Heat shock (+6°C) induced transient elevation in HSP90 mRNA in trout hepatocytes, peaking within 10-h post-HS, and remained elevated over a 24-h period. Cortisol did not modify the unstimulated expression of HSP90 mRNA, whereas the HS-induced HSP90 mRNA expression was attenuated in trout hepatocytes. 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| subjects | Amino Acid Sequence Animals Cells, Cultured Cortisol Fish Gene Expression Regulation - drug effects Glucocorticoid receptors Heat shock protein Hepatocyte primary cultures Hepatocytes - cytology Hepatocytes - drug effects Hepatocytes - metabolism HSP90 Heat-Shock Proteins - chemistry HSP90 Heat-Shock Proteins - genetics HSP90 mRNA Hydrocortisone - pharmacology Molecular Sequence Data Oncorhynchus mykiss Oncorhynchus mykiss - genetics Rainbow trout RNA, Messenger - genetics RNA, Messenger - metabolism Stress Temperature shock Time Factors |
| title | Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes |
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