Mitigation of bioprosthetic heart valve degeneration through biocompatibility: in vitro versus spontaneous endothelialization
Background: Glutaraldehyde-related cytotoxicity and transanastomotic ingrowth inhibition prevent the spontaneous endothelialization of bioprosthetic heart valves. In order to evaluate the ability of improved biocompatibility to reduce tissue degeneration, conventionally fixed aortic root prostheses...
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| Veröffentlicht in: | Biomaterials 2001-07, Vol.22 (13), p.1837-1846 |
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| creator | Trantina-Yates, Ameli E Human, Paul Bracher, Mona Zilla, Peter |
| description | Background: Glutaraldehyde-related cytotoxicity and transanastomotic ingrowth inhibition prevent the spontaneous endothelialization of bioprosthetic heart valves. In order to evaluate the ability of improved biocompatibility to reduce tissue degeneration, conventionally fixed aortic root prostheses were both glutaraldehyde-detoxified and in vitro endothelialized.
Methods: Entire aortic roots were fixed in 0.2% glutaraldehyde (GA) (control group) and either detoxified in acetic acid-buffered urazole (0.1
m) or detoxified and in vitro lined with cultured, autologous jugular vein endothelial cells. The valved roots were inserted in the distal aortic arch of 15 juvenile Merino sheep for a period of 12 weeks. Upon explant, leaflets, sinuses and aortic wall of the prostheses were analysed by SEM to assess the surface endothelium, histologically regarding tissue inflammation, and by atomic absorption spectrophotometry to determine the content of tissue calcium.
Results: There was no endothelium on control grafts, except for a short anastomotic pannus. The detoxified group showed an incomplete patchy endothelium on the aortic wall but hardly any on the leaflets, whereas, the in vitro lined group had aortic wall, sinuses and most of the leaflets confluently endothelialized. Tissue inflammation was prominent in the control group and least expressed in the endothelialized group (
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| doi_str_mv | 10.1016/S0142-9612(00)00365-3 |
| format | Article |
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Methods: Entire aortic roots were fixed in 0.2% glutaraldehyde (GA) (control group) and either detoxified in acetic acid-buffered urazole (0.1
m) or detoxified and in vitro lined with cultured, autologous jugular vein endothelial cells. The valved roots were inserted in the distal aortic arch of 15 juvenile Merino sheep for a period of 12 weeks. Upon explant, leaflets, sinuses and aortic wall of the prostheses were analysed by SEM to assess the surface endothelium, histologically regarding tissue inflammation, and by atomic absorption spectrophotometry to determine the content of tissue calcium.
Results: There was no endothelium on control grafts, except for a short anastomotic pannus. The detoxified group showed an incomplete patchy endothelium on the aortic wall but hardly any on the leaflets, whereas, the in vitro lined group had aortic wall, sinuses and most of the leaflets confluently endothelialized. Tissue inflammation was prominent in the control group and least expressed in the endothelialized group (
p<0.05). Detoxification significantly reduced leaflet calcification. In the aortic wall, both detoxification and endothelial lining were required to significantly mitigate calcification.
Conclusion: In the 12 week circulatory sheep model, the calcium mitigating effect of detoxification was more pronounced than that of in vitro endothelialization. Nevertheless, there was a distinct overall benefit if detoxification was combined with endothelialization.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/S0142-9612(00)00365-3</identifier><identifier>PMID: 11396888</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biocompatibility ; Biocompatible Materials ; Biological and medical sciences ; Calcification ; Cardiovascular system ; Cell culture ; Cell Division ; Endothelialization ; Endothelium, Vascular - cytology ; Endothelium, Vascular - ultrastructure ; Heart Valve Prosthesis ; Heart valves ; In Vitro Techniques ; Medical sciences ; Microscopy, Electron, Scanning ; Root prostheses ; Spectrophotometry, Atomic ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the heart ; Swine ; Technology. Biomaterials. Equipments ; Toxicity</subject><ispartof>Biomaterials, 2001-07, Vol.22 (13), p.1837-1846</ispartof><rights>2001 Elsevier Science Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-288161a2f7699c11912bb47ceac6f033dd268de683f0ef5b88fecf7d8af67a4d3</citedby><cites>FETCH-LOGICAL-c451t-288161a2f7699c11912bb47ceac6f033dd268de683f0ef5b88fecf7d8af67a4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961200003653$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=998294$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11396888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Trantina-Yates, Ameli E</creatorcontrib><creatorcontrib>Human, Paul</creatorcontrib><creatorcontrib>Bracher, Mona</creatorcontrib><creatorcontrib>Zilla, Peter</creatorcontrib><title>Mitigation of bioprosthetic heart valve degeneration through biocompatibility: in vitro versus spontaneous endothelialization</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Background: Glutaraldehyde-related cytotoxicity and transanastomotic ingrowth inhibition prevent the spontaneous endothelialization of bioprosthetic heart valves. In order to evaluate the ability of improved biocompatibility to reduce tissue degeneration, conventionally fixed aortic root prostheses were both glutaraldehyde-detoxified and in vitro endothelialized.
Methods: Entire aortic roots were fixed in 0.2% glutaraldehyde (GA) (control group) and either detoxified in acetic acid-buffered urazole (0.1
m) or detoxified and in vitro lined with cultured, autologous jugular vein endothelial cells. The valved roots were inserted in the distal aortic arch of 15 juvenile Merino sheep for a period of 12 weeks. Upon explant, leaflets, sinuses and aortic wall of the prostheses were analysed by SEM to assess the surface endothelium, histologically regarding tissue inflammation, and by atomic absorption spectrophotometry to determine the content of tissue calcium.
Results: There was no endothelium on control grafts, except for a short anastomotic pannus. The detoxified group showed an incomplete patchy endothelium on the aortic wall but hardly any on the leaflets, whereas, the in vitro lined group had aortic wall, sinuses and most of the leaflets confluently endothelialized. Tissue inflammation was prominent in the control group and least expressed in the endothelialized group (
p<0.05). Detoxification significantly reduced leaflet calcification. In the aortic wall, both detoxification and endothelial lining were required to significantly mitigate calcification.
Conclusion: In the 12 week circulatory sheep model, the calcium mitigating effect of detoxification was more pronounced than that of in vitro endothelialization. Nevertheless, there was a distinct overall benefit if detoxification was combined with endothelialization.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials</subject><subject>Biological and medical sciences</subject><subject>Calcification</subject><subject>Cardiovascular system</subject><subject>Cell culture</subject><subject>Cell Division</subject><subject>Endothelialization</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - ultrastructure</subject><subject>Heart Valve Prosthesis</subject><subject>Heart valves</subject><subject>In Vitro Techniques</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Root prostheses</subject><subject>Spectrophotometry, Atomic</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the heart</subject><subject>Swine</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Toxicity</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuLFDEUhYMoTs_oT1ACgoyL0qQeqWQ2IoMvGHGhrkMquem-Ul1pk3TBCP53013NuBxXefDl3JNzCHnG2WvOuHjzjfG2rpTg9SVjrxhrRFc1D8iKy15WnWLdQ7K6Q87IeUo_WTmztn5MzjhvlJBSrsifL5hxbTKGiQZPBwy7GFLeQEZLN2BiprMZZ6AO1jBBXMi8iWG_3hxwG7a7cjngiPn2iuJEZ8wx0Bli2ieadmHKZoJQ9jC5UJRHNCP-Pgo9IY-8GRM8Pa0X5MeH99-vP1U3Xz9-vn53U9m247mqpeSCm9r3QinLueL1MLS9BWOFZ03jXC2kAyEbz8B3g5QerO-dNF70pnXNBXm56JbP_dpDynqLycI4Ls50zxTvlGzuBcucEnD_HyBvRfHZFbBbQFtyTRG83kXcmnirOdOHJvWxSX2oSTOmj03qw4DnpwH7YQvu36tTdQV4cQJMsmb00UwW0x2nlKxVW6i3CwUl3hkh6mQRJgsOI9isXcB7jPwF4zO-Hw</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Trantina-Yates, Ameli E</creator><creator>Human, Paul</creator><creator>Bracher, Mona</creator><creator>Zilla, Peter</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>Mitigation of bioprosthetic heart valve degeneration through biocompatibility: in vitro versus spontaneous endothelialization</title><author>Trantina-Yates, Ameli E ; Human, Paul ; Bracher, Mona ; Zilla, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-288161a2f7699c11912bb47ceac6f033dd268de683f0ef5b88fecf7d8af67a4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials</topic><topic>Biological and medical sciences</topic><topic>Calcification</topic><topic>Cardiovascular system</topic><topic>Cell culture</topic><topic>Cell Division</topic><topic>Endothelialization</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - ultrastructure</topic><topic>Heart Valve Prosthesis</topic><topic>Heart valves</topic><topic>In Vitro Techniques</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Root prostheses</topic><topic>Spectrophotometry, Atomic</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the heart</topic><topic>Swine</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trantina-Yates, Ameli E</creatorcontrib><creatorcontrib>Human, Paul</creatorcontrib><creatorcontrib>Bracher, Mona</creatorcontrib><creatorcontrib>Zilla, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trantina-Yates, Ameli E</au><au>Human, Paul</au><au>Bracher, Mona</au><au>Zilla, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitigation of bioprosthetic heart valve degeneration through biocompatibility: in vitro versus spontaneous endothelialization</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>22</volume><issue>13</issue><spage>1837</spage><epage>1846</epage><pages>1837-1846</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Background: Glutaraldehyde-related cytotoxicity and transanastomotic ingrowth inhibition prevent the spontaneous endothelialization of bioprosthetic heart valves. In order to evaluate the ability of improved biocompatibility to reduce tissue degeneration, conventionally fixed aortic root prostheses were both glutaraldehyde-detoxified and in vitro endothelialized.
Methods: Entire aortic roots were fixed in 0.2% glutaraldehyde (GA) (control group) and either detoxified in acetic acid-buffered urazole (0.1
m) or detoxified and in vitro lined with cultured, autologous jugular vein endothelial cells. The valved roots were inserted in the distal aortic arch of 15 juvenile Merino sheep for a period of 12 weeks. Upon explant, leaflets, sinuses and aortic wall of the prostheses were analysed by SEM to assess the surface endothelium, histologically regarding tissue inflammation, and by atomic absorption spectrophotometry to determine the content of tissue calcium.
Results: There was no endothelium on control grafts, except for a short anastomotic pannus. The detoxified group showed an incomplete patchy endothelium on the aortic wall but hardly any on the leaflets, whereas, the in vitro lined group had aortic wall, sinuses and most of the leaflets confluently endothelialized. Tissue inflammation was prominent in the control group and least expressed in the endothelialized group (
p<0.05). Detoxification significantly reduced leaflet calcification. In the aortic wall, both detoxification and endothelial lining were required to significantly mitigate calcification.
Conclusion: In the 12 week circulatory sheep model, the calcium mitigating effect of detoxification was more pronounced than that of in vitro endothelialization. Nevertheless, there was a distinct overall benefit if detoxification was combined with endothelialization.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11396888</pmid><doi>10.1016/S0142-9612(00)00365-3</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biocompatibility Biocompatible Materials Biological and medical sciences Calcification Cardiovascular system Cell culture Cell Division Endothelialization Endothelium, Vascular - cytology Endothelium, Vascular - ultrastructure Heart Valve Prosthesis Heart valves In Vitro Techniques Medical sciences Microscopy, Electron, Scanning Root prostheses Spectrophotometry, Atomic Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the heart Swine Technology. Biomaterials. Equipments Toxicity |
| title | Mitigation of bioprosthetic heart valve degeneration through biocompatibility: in vitro versus spontaneous endothelialization |
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