Interaction between cultured endothelial cells and macrophages: In Vitro model for studying flavonoids in redox-dependent gene expression
This article focused on two methods to measure the activity of NF-kB. Both methods evalute "post-IkB phosphorylation" stages in the NF-kB activation cascade. In fact, EMSA performed with nuclear extracts provides an information only on NF-kB nuclear translocation and its ability to bind kB...
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| Veröffentlicht in: | Methods in Enzymology 2001, Vol.335, p.387-397 |
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| creator | Rimbach, Gerald Saliou, Claude Canali, Raffaella Virgili, Fabio |
| description | This article focused on two methods to measure the activity of NF-kB. Both methods evalute "post-IkB phosphorylation" stages in the NF-kB activation cascade. In fact, EMSA performed with nuclear extracts provides an information only on NF-kB nuclear translocation and its ability to bind kB-DNA sequences. Likewise, the reporter gene assay is limited to assessing NF-kB-dependent gene expression no matter the mechanism that originally activated NF-kB. Nevertheless, the latter assay represents a more physiological and more reproducible way of measuring NF-kB activity in mammalian cells than the EMSA does. In order to obtain further insights into NF-kB signal transduction pathways, investigating IkB degradation and phosphorylation are recommended. The cloning and characterization of IkB kinases provided new testing possibilities based on measure of their activity. |
| doi_str_mv | 10.1016/S0076-6879(01)35261-8 |
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The cloning and characterization of IkB kinases provided new testing possibilities based on measure of their activity.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Endothelium - cytology</subject><subject>Flavonoids - pharmacology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Macrophages - cytology</subject><subject>Mice</subject><subject>NF-kappa B - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Oxidative Stress</subject><subject>Reactive Oxygen Species</subject><subject>Transcriptional Activation - drug effects</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>9780121822361</isbn><isbn>0121822362</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc1u1TAQhS1-RK_KfQSQV4guAh77OnHYIFRRuFIlFvxsLcee3BoldrCd0j4Cb41vW5jNbL45M2cOIS-AvQEG7duvjHVt06quf83gTEjeQqMekQ1I2TVdr9Rjsu07xYCD4ly08IRs_o-ckG3OP1mtive8e0ZOAHaMCaU25M8-FEzGFh8DHbD8RgzUrlNZEzqKwcVyhZM3E7U4TZma4OhsbIrLlTlgfkf3gf7wJUU6R4cTHWOiuazu1ocDHSdzHUP0LlMfaBWMN43DpapiKPSAASneLAlzrtufk6ejmTJuH_op-X7x8dv55-byy6f9-YfLxgqQpcHdIG0_tD0YPtqht9V027dCcDcCZ0qKnXUKOjd2yjhrBq6YYHKUwJRQ0opT8uped0nx14q56NnnozkTMK5Zd6wHqaSs4MsHcB1mdHpJfjbpVv97XgXe3wNYz732mHS2HoNF5xPaol30Gpg-JqjvEtTHODQDfZegVuIvo9OMiA</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Rimbach, Gerald</creator><creator>Saliou, Claude</creator><creator>Canali, Raffaella</creator><creator>Virgili, Fabio</creator><general>Elsevier Science & Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2001</creationdate><title>Interaction between cultured endothelial cells and macrophages: In Vitro model for studying flavonoids in redox-dependent gene expression</title><author>Rimbach, Gerald ; Saliou, Claude ; Canali, Raffaella ; Virgili, Fabio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-e4b5c9b691a2fcb9c012696332df1208534cd817df78adcab280305f5108385c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Coculture Techniques</topic><topic>Endothelium - cytology</topic><topic>Flavonoids - pharmacology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Macrophages - cytology</topic><topic>Mice</topic><topic>NF-kappa B - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Oxidative Stress</topic><topic>Reactive Oxygen Species</topic><topic>Transcriptional Activation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rimbach, Gerald</creatorcontrib><creatorcontrib>Saliou, Claude</creatorcontrib><creatorcontrib>Canali, Raffaella</creatorcontrib><creatorcontrib>Virgili, Fabio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in Enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rimbach, Gerald</au><au>Saliou, Claude</au><au>Canali, Raffaella</au><au>Virgili, Fabio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction between cultured endothelial cells and macrophages: In Vitro model for studying flavonoids in redox-dependent gene expression</atitle><jtitle>Methods in Enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>2001</date><risdate>2001</risdate><volume>335</volume><spage>387</spage><epage>397</epage><pages>387-397</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>9780121822361</isbn><isbn>0121822362</isbn><abstract>This article focused on two methods to measure the activity of NF-kB. 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| ispartof | Methods in Enzymology, 2001, Vol.335, p.387-397 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; ScienceDirect eBooks |
| subjects | Animals Cells, Cultured Coculture Techniques Endothelium - cytology Flavonoids - pharmacology Gene Expression Regulation - drug effects Humans Macrophages - cytology Mice NF-kappa B - metabolism Oxidation-Reduction Oxidative Stress Reactive Oxygen Species Transcriptional Activation - drug effects |
| title | Interaction between cultured endothelial cells and macrophages: In Vitro model for studying flavonoids in redox-dependent gene expression |
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