Sperm Antigen 6 Is the Murine Homologue of the Chlamydomonas reinhardtii Central Apparatus Protein Encoded by the PF16 Locus
A cDNA encoding sperm antigen 6 (Spag6), the murine homologue of the Chlamydomonas reinhardtii PF16 proteinâa component of the flagella central apparatusâwas isolated from a mouse testis cDNA library. The cDNA sequence predicted a 55.3-kDa polypeptide containing 8 contiguous armadillo repeats wi...
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| Veröffentlicht in: | Biology of reproduction 2000-03, Vol.62 (3), p.511-518 |
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| container_title | Biology of reproduction |
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| creator | SAPIRO, R TARANTINO, L. M VELAZQUEZ, F KIRIAKIDOU, M HECHT, N. B BUCAN, M STRAUSS, J. F |
| description | A cDNA encoding sperm antigen 6 (Spag6), the murine homologue of the Chlamydomonas reinhardtii PF16 proteinâa component of the flagella central apparatusâwas isolated from a mouse testis cDNA library. The cDNA sequence
predicted a 55.3-kDa polypeptide containing 8 contiguous armadillo repeats with 65% amino acid sequence identity and 81% similarity
to the Chlamydomonas PF1 protein. An antipeptide antibody generated against a C-terminal sequence recognized a 55-kDa protein in sperm extracts
and localized Spag6 to the principal piece of permeabilized mouse sperm tails. When expressed in COS-1 cells, Spag6 colocalized
with microtubules. The Spag6 gene was found to be highly expressed in testis and was mapped using the T31 radiation hybrid panel to mouse chromosome 16.
Mutations in the Chlamydomonas PF16 gene cause flagellar paralysis. The presence of a highly conserved mammalian PF16 homologue (Spag6) raises the possibility
that Spag6 plays an important role in sperm flagellar function. |
| doi_str_mv | 10.1095/biolreprod62.3.511 |
| format | Article |
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predicted a 55.3-kDa polypeptide containing 8 contiguous armadillo repeats with 65% amino acid sequence identity and 81% similarity
to the Chlamydomonas PF1 protein. An antipeptide antibody generated against a C-terminal sequence recognized a 55-kDa protein in sperm extracts
and localized Spag6 to the principal piece of permeabilized mouse sperm tails. When expressed in COS-1 cells, Spag6 colocalized
with microtubules. The Spag6 gene was found to be highly expressed in testis and was mapped using the T31 radiation hybrid panel to mouse chromosome 16.
Mutations in the Chlamydomonas PF16 gene cause flagellar paralysis. The presence of a highly conserved mammalian PF16 homologue (Spag6) raises the possibility
that Spag6 plays an important role in sperm flagellar function.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod62.3.511</identifier><identifier>PMID: 10684790</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Algal Proteins ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Western ; Chlamydomonas reinhardtii ; Chromosome Mapping ; COS Cells - metabolism ; DNA, Complementary ; Flagella - metabolism ; Fundamental and applied biological sciences. Psychology ; Male ; Mammalian male genital system ; Mice ; Microtubule Proteins - genetics ; Microtubule Proteins - immunology ; Microtubule Proteins - metabolism ; Molecular Sequence Data ; Morphology. Physiology ; Organ Specificity ; Sequence Analysis, Protein ; Sequence Homology, Amino Acid ; Spermatozoa - immunology ; Spermatozoa - metabolism ; Testis - metabolism ; Transfection ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2000-03, Vol.62 (3), p.511-518</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1289659$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10684790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SAPIRO, R</creatorcontrib><creatorcontrib>TARANTINO, L. M</creatorcontrib><creatorcontrib>VELAZQUEZ, F</creatorcontrib><creatorcontrib>KIRIAKIDOU, M</creatorcontrib><creatorcontrib>HECHT, N. B</creatorcontrib><creatorcontrib>BUCAN, M</creatorcontrib><creatorcontrib>STRAUSS, J. F</creatorcontrib><title>Sperm Antigen 6 Is the Murine Homologue of the Chlamydomonas reinhardtii Central Apparatus Protein Encoded by the PF16 Locus</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>A cDNA encoding sperm antigen 6 (Spag6), the murine homologue of the Chlamydomonas reinhardtii PF16 proteinâa component of the flagella central apparatusâwas isolated from a mouse testis cDNA library. The cDNA sequence
predicted a 55.3-kDa polypeptide containing 8 contiguous armadillo repeats with 65% amino acid sequence identity and 81% similarity
to the Chlamydomonas PF1 protein. An antipeptide antibody generated against a C-terminal sequence recognized a 55-kDa protein in sperm extracts
and localized Spag6 to the principal piece of permeabilized mouse sperm tails. When expressed in COS-1 cells, Spag6 colocalized
with microtubules. The Spag6 gene was found to be highly expressed in testis and was mapped using the T31 radiation hybrid panel to mouse chromosome 16.
Mutations in the Chlamydomonas PF16 gene cause flagellar paralysis. The presence of a highly conserved mammalian PF16 homologue (Spag6) raises the possibility
that Spag6 plays an important role in sperm flagellar function.</description><subject>Algal Proteins</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Chlamydomonas reinhardtii</subject><subject>Chromosome Mapping</subject><subject>COS Cells - metabolism</subject><subject>DNA, Complementary</subject><subject>Flagella - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mice</subject><subject>Microtubule Proteins - genetics</subject><subject>Microtubule Proteins - immunology</subject><subject>Microtubule Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>Organ Specificity</subject><subject>Sequence Analysis, Protein</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spermatozoa - immunology</subject><subject>Spermatozoa - metabolism</subject><subject>Testis - metabolism</subject><subject>Transfection</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0U1r3DAQBmBRGprNtn-gh6JD25s3-rAl67gsSRPYkkDbsxlb47WKbLmSzbKQH1832dDTwMvDOzBDyEfONpyZ4rp2wUccY7BKbOSm4PwNWfFCmEwLVb4lK8aYyqRU8pJcpfSbMZ5LId-RS85UmWvDVuTpx4ixp9thcgccqKL3iU4d0u9zdAPSu9AHHw4z0tA-57vOQ3-ySzxAohHd0EG0k3N0h8MUwdPtOEKEaU70MYZpAfRmaIJFS-vTc8XjLVd0H5o5vScXLfiEH85zTX7d3vzc3WX7h2_3u-0-64RSU6YLVULNWpYXAkwDUOiy5VogaiGNbsFqLC1jAlhusNSa5bkqjNS1zYU1Qq7J15fe5VZ_ZkxT1bvUoPcwYJhTpZnhRVmwBX46w7nu0VZjdD3EU_V6rwV8PgNIDfg2wtC49N-J0vzbvCZfXljnDt3RRaxSD94vrbI6Ho9KVLJa3iX_AmiXiEQ</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>SAPIRO, R</creator><creator>TARANTINO, L. M</creator><creator>VELAZQUEZ, F</creator><creator>KIRIAKIDOU, M</creator><creator>HECHT, N. B</creator><creator>BUCAN, M</creator><creator>STRAUSS, J. F</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Sperm Antigen 6 Is the Murine Homologue of the Chlamydomonas reinhardtii Central Apparatus Protein Encoded by the PF16 Locus</title><author>SAPIRO, R ; TARANTINO, L. M ; VELAZQUEZ, F ; KIRIAKIDOU, M ; HECHT, N. B ; BUCAN, M ; STRAUSS, J. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h266t-7568ab0f0452a9caa578f172ee72397fad7e8d002a049e87704465937bd42d923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Algal Proteins</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Chlamydomonas reinhardtii</topic><topic>Chromosome Mapping</topic><topic>COS Cells - metabolism</topic><topic>DNA, Complementary</topic><topic>Flagella - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mice</topic><topic>Microtubule Proteins - genetics</topic><topic>Microtubule Proteins - immunology</topic><topic>Microtubule Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>Organ Specificity</topic><topic>Sequence Analysis, Protein</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spermatozoa - immunology</topic><topic>Spermatozoa - metabolism</topic><topic>Testis - metabolism</topic><topic>Transfection</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SAPIRO, R</creatorcontrib><creatorcontrib>TARANTINO, L. M</creatorcontrib><creatorcontrib>VELAZQUEZ, F</creatorcontrib><creatorcontrib>KIRIAKIDOU, M</creatorcontrib><creatorcontrib>HECHT, N. B</creatorcontrib><creatorcontrib>BUCAN, M</creatorcontrib><creatorcontrib>STRAUSS, J. F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SAPIRO, R</au><au>TARANTINO, L. M</au><au>VELAZQUEZ, F</au><au>KIRIAKIDOU, M</au><au>HECHT, N. B</au><au>BUCAN, M</au><au>STRAUSS, J. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm Antigen 6 Is the Murine Homologue of the Chlamydomonas reinhardtii Central Apparatus Protein Encoded by the PF16 Locus</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>62</volume><issue>3</issue><spage>511</spage><epage>518</epage><pages>511-518</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>A cDNA encoding sperm antigen 6 (Spag6), the murine homologue of the Chlamydomonas reinhardtii PF16 proteinâa component of the flagella central apparatusâwas isolated from a mouse testis cDNA library. The cDNA sequence
predicted a 55.3-kDa polypeptide containing 8 contiguous armadillo repeats with 65% amino acid sequence identity and 81% similarity
to the Chlamydomonas PF1 protein. An antipeptide antibody generated against a C-terminal sequence recognized a 55-kDa protein in sperm extracts
and localized Spag6 to the principal piece of permeabilized mouse sperm tails. When expressed in COS-1 cells, Spag6 colocalized
with microtubules. The Spag6 gene was found to be highly expressed in testis and was mapped using the T31 radiation hybrid panel to mouse chromosome 16.
Mutations in the Chlamydomonas PF16 gene cause flagellar paralysis. The presence of a highly conserved mammalian PF16 homologue (Spag6) raises the possibility
that Spag6 plays an important role in sperm flagellar function.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>10684790</pmid><doi>10.1095/biolreprod62.3.511</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2000-03, Vol.62 (3), p.511-518 |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Algal Proteins Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Western Chlamydomonas reinhardtii Chromosome Mapping COS Cells - metabolism DNA, Complementary Flagella - metabolism Fundamental and applied biological sciences. Psychology Male Mammalian male genital system Mice Microtubule Proteins - genetics Microtubule Proteins - immunology Microtubule Proteins - metabolism Molecular Sequence Data Morphology. Physiology Organ Specificity Sequence Analysis, Protein Sequence Homology, Amino Acid Spermatozoa - immunology Spermatozoa - metabolism Testis - metabolism Transfection Vertebrates: reproduction |
| title | Sperm Antigen 6 Is the Murine Homologue of the Chlamydomonas reinhardtii Central Apparatus Protein Encoded by the PF16 Locus |
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