The Metal Dependence of Bacillus subtilis Phytase

The metal ion requirement of a Bacillus subtilis phytase has been studied. Removal of metal ions from the enzyme by EDTA resulted in complete inactivation. Circular dichroism spectroscopy was used to study the effect of metal ion removal on the protein conformation. The loss of enzymatic activity is...

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Veröffentlicht in:	Biochemical and biophysical research communications 2000-02, Vol.268 (2), p.365-369
Hauptverfasser:	Kerovuo, Janne, Lappalainen, Ilkka, Reinikainen, Tapani
Format:	Artikel
Sprache:	eng
Schlagworte:	6-Phytase - metabolism Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - metabolism Calcium - metabolism Circular Dichroism Endopeptidases - metabolism Enzyme Activation Enzyme Stability Metals - metabolism Protein Conformation
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