Three forms of GnRH in the brain and pituitary of the turbot, Scophthalmus maximus: immunological characterization and seasonal variation
Three forms of GnRH, chicken (c) GnRH-II, salmon (s) and seabream (sb) GnRH, were immunologically characterized in the brain and pituitary of turbot by ELISA. cGnRH-II and sGnRH were detected in the brain, while sbGnRH and sGnRH (but not cGnRH-II) were detected in the pituitary. In females, the leve...
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| Veröffentlicht in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2001-06, Vol.129 (2-3), p.551-558 |
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| container_title | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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| creator | Andersson, Eva Fjelldal, Per Gunnar Klenke, Ulrike Vikingstad, Erik Taranger, Geir Lasse Zohar, Yonathan Stefansson, Sigurd Olav |
| description | Three forms of GnRH, chicken (c) GnRH-II, salmon (s) and seabream (sb) GnRH, were immunologically characterized in the brain and pituitary of turbot by ELISA. cGnRH-II and sGnRH were detected in the brain, while sbGnRH and sGnRH (but not cGnRH-II) were detected in the pituitary. In females, the levels of cGnRH-II in the turbot brain extracts increased from May to July, concomitant with an increase in oocyte diameter. In the pituitary, sbGnRH was found to be the dominant form, with levels 100–600-fold those of sGnRH. Both sGnRH and sbGnRH in the pituitary showed variation during the spawning season; sbGnRH increased from May to July and correlated with the increase in oocyte diameter, while sGnRH decreased. The overall patterns were the same for male turbot, although levels were generally lower. These findings suggest that sbGnRH could be controlling reproduction in the turbot. However, the seasonal variation in sGnRH indicates a potential physiological role in turbot reproduction. This study gives the first immunological indications that sbGnRH is present in the pituitary of a pleuronectiform fish, and will provide the basis for further studies on the endocrine regulation of reproduction in flatfish. |
| doi_str_mv | 10.1016/S1096-4959(01)00363-3 |
| format | Article |
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In females, the levels of cGnRH-II in the turbot brain extracts increased from May to July, concomitant with an increase in oocyte diameter. In the pituitary, sbGnRH was found to be the dominant form, with levels 100–600-fold those of sGnRH. Both sGnRH and sbGnRH in the pituitary showed variation during the spawning season; sbGnRH increased from May to July and correlated with the increase in oocyte diameter, while sGnRH decreased. The overall patterns were the same for male turbot, although levels were generally lower. These findings suggest that sbGnRH could be controlling reproduction in the turbot. However, the seasonal variation in sGnRH indicates a potential physiological role in turbot reproduction. This study gives the first immunological indications that sbGnRH is present in the pituitary of a pleuronectiform fish, and will provide the basis for further studies on the endocrine regulation of reproduction in flatfish.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/S1096-4959(01)00363-3</identifier><identifier>PMID: 11399491</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; Brain ; Brain - metabolism ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Female ; Fish ; Flatfishes - metabolism ; Flatfishes - physiology ; GnRH ; Gonadotropin-Releasing Hormone - immunology ; Gonadotropin-Releasing Hormone - metabolism ; Male ; Marine ; Pituitary ; Pituitary Gland - metabolism ; Protein Isoforms - immunology ; Protein Isoforms - metabolism ; Scophthalmus maximus ; Seasons ; Turbot, Scophthalmus maximus</subject><ispartof>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2001-06, Vol.129 (2-3), p.551-558</ispartof><rights>2001 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-f159307b3fab9b8b02701b5d4fe277a3f950cda970deaae41eb5bce77bbf82d83</citedby><cites>FETCH-LOGICAL-c515t-f159307b3fab9b8b02701b5d4fe277a3f950cda970deaae41eb5bce77bbf82d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1096-4959(01)00363-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11399491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andersson, Eva</creatorcontrib><creatorcontrib>Fjelldal, Per Gunnar</creatorcontrib><creatorcontrib>Klenke, Ulrike</creatorcontrib><creatorcontrib>Vikingstad, Erik</creatorcontrib><creatorcontrib>Taranger, Geir Lasse</creatorcontrib><creatorcontrib>Zohar, Yonathan</creatorcontrib><creatorcontrib>Stefansson, Sigurd Olav</creatorcontrib><title>Three forms of GnRH in the brain and pituitary of the turbot, Scophthalmus maximus: immunological characterization and seasonal variation</title><title>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</title><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><description>Three forms of GnRH, chicken (c) GnRH-II, salmon (s) and seabream (sb) GnRH, were immunologically characterized in the brain and pituitary of turbot by ELISA. cGnRH-II and sGnRH were detected in the brain, while sbGnRH and sGnRH (but not cGnRH-II) were detected in the pituitary. In females, the levels of cGnRH-II in the turbot brain extracts increased from May to July, concomitant with an increase in oocyte diameter. In the pituitary, sbGnRH was found to be the dominant form, with levels 100–600-fold those of sGnRH. Both sGnRH and sbGnRH in the pituitary showed variation during the spawning season; sbGnRH increased from May to July and correlated with the increase in oocyte diameter, while sGnRH decreased. The overall patterns were the same for male turbot, although levels were generally lower. These findings suggest that sbGnRH could be controlling reproduction in the turbot. However, the seasonal variation in sGnRH indicates a potential physiological role in turbot reproduction. This study gives the first immunological indications that sbGnRH is present in the pituitary of a pleuronectiform fish, and will provide the basis for further studies on the endocrine regulation of reproduction in flatfish.</description><subject>Animals</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fish</subject><subject>Flatfishes - metabolism</subject><subject>Flatfishes - physiology</subject><subject>GnRH</subject><subject>Gonadotropin-Releasing Hormone - immunology</subject><subject>Gonadotropin-Releasing Hormone - metabolism</subject><subject>Male</subject><subject>Marine</subject><subject>Pituitary</subject><subject>Pituitary Gland - metabolism</subject><subject>Protein Isoforms - immunology</subject><subject>Protein Isoforms - metabolism</subject><subject>Scophthalmus maximus</subject><subject>Seasons</subject><subject>Turbot, Scophthalmus maximus</subject><issn>1096-4959</issn><issn>1879-1107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEoqXwCIBPqEgEPHGcxFwQqqBFqoTEtmfLdsaNURJvbacC3oC3xrtZxI0eRjPyfPN7NH9RPAf6Fig07zZARVPWgotTCq8pZQ0r2YPiGLpWlAC0fZjrv8hR8STG7xnqgMHj4giACVELOC5-Xw0BkVgfpki8JefztwviZpIGJDqoXKm5J1uXFpdU-LlDdq20BO3TG7IxfjukQY3TEsmkfric3xM3TcvsR3_jjBqJGVRQJmFwv1RyflWMqKKfc_dOBbd_flo8smqM-OyQT4rrz5-uzi7Ky6_nX84-XpaGA0-lBS4YbTWzSgvdaVq1FDTva4tV2ypmBaemV6KlPSqFNaDm2mDbam27qu_YSfFq1d0Gf7tgTHJy0eA4qhn9EmVLBdRCVPeC0LGO87rO4On_wabhdddRwTPKV9QEH2NAK7fBTfmwEqjc-Sr3vsqdaZKC3PsqWZ57cfhi0RP2_6YORmbg5QpY5aW6CS7K602VBWkOaDqRiQ8rgfm4dw6DjMbhbLB3AU2SvXf3LPEHvEm-Tg</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Andersson, Eva</creator><creator>Fjelldal, Per Gunnar</creator><creator>Klenke, Ulrike</creator><creator>Vikingstad, Erik</creator><creator>Taranger, Geir Lasse</creator><creator>Zohar, Yonathan</creator><creator>Stefansson, Sigurd Olav</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Three forms of GnRH in the brain and pituitary of the turbot, Scophthalmus maximus: immunological characterization and seasonal variation</title><author>Andersson, Eva ; Fjelldal, Per Gunnar ; Klenke, Ulrike ; Vikingstad, Erik ; Taranger, Geir Lasse ; Zohar, Yonathan ; Stefansson, Sigurd Olav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-f159307b3fab9b8b02701b5d4fe277a3f950cda970deaae41eb5bce77bbf82d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Brain</topic><topic>Brain - metabolism</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fish</topic><topic>Flatfishes - metabolism</topic><topic>Flatfishes - physiology</topic><topic>GnRH</topic><topic>Gonadotropin-Releasing Hormone - immunology</topic><topic>Gonadotropin-Releasing Hormone - metabolism</topic><topic>Male</topic><topic>Marine</topic><topic>Pituitary</topic><topic>Pituitary Gland - metabolism</topic><topic>Protein Isoforms - immunology</topic><topic>Protein Isoforms - metabolism</topic><topic>Scophthalmus maximus</topic><topic>Seasons</topic><topic>Turbot, Scophthalmus maximus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andersson, Eva</creatorcontrib><creatorcontrib>Fjelldal, Per Gunnar</creatorcontrib><creatorcontrib>Klenke, Ulrike</creatorcontrib><creatorcontrib>Vikingstad, Erik</creatorcontrib><creatorcontrib>Taranger, Geir Lasse</creatorcontrib><creatorcontrib>Zohar, Yonathan</creatorcontrib><creatorcontrib>Stefansson, Sigurd Olav</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andersson, Eva</au><au>Fjelldal, Per Gunnar</au><au>Klenke, Ulrike</au><au>Vikingstad, Erik</au><au>Taranger, Geir Lasse</au><au>Zohar, Yonathan</au><au>Stefansson, Sigurd Olav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Three forms of GnRH in the brain and pituitary of the turbot, Scophthalmus maximus: immunological characterization and seasonal variation</atitle><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>129</volume><issue>2-3</issue><spage>551</spage><epage>558</epage><pages>551-558</pages><issn>1096-4959</issn><eissn>1879-1107</eissn><abstract>Three forms of GnRH, chicken (c) GnRH-II, salmon (s) and seabream (sb) GnRH, were immunologically characterized in the brain and pituitary of turbot by ELISA. cGnRH-II and sGnRH were detected in the brain, while sbGnRH and sGnRH (but not cGnRH-II) were detected in the pituitary. In females, the levels of cGnRH-II in the turbot brain extracts increased from May to July, concomitant with an increase in oocyte diameter. In the pituitary, sbGnRH was found to be the dominant form, with levels 100–600-fold those of sGnRH. Both sGnRH and sbGnRH in the pituitary showed variation during the spawning season; sbGnRH increased from May to July and correlated with the increase in oocyte diameter, while sGnRH decreased. The overall patterns were the same for male turbot, although levels were generally lower. These findings suggest that sbGnRH could be controlling reproduction in the turbot. However, the seasonal variation in sGnRH indicates a potential physiological role in turbot reproduction. This study gives the first immunological indications that sbGnRH is present in the pituitary of a pleuronectiform fish, and will provide the basis for further studies on the endocrine regulation of reproduction in flatfish.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>11399491</pmid><doi>10.1016/S1096-4959(01)00363-3</doi><tpages>8</tpages></addata></record> |
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| ispartof | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2001-06, Vol.129 (2-3), p.551-558 |
| issn | 1096-4959 1879-1107 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Brain Brain - metabolism ELISA Enzyme-Linked Immunosorbent Assay Female Fish Flatfishes - metabolism Flatfishes - physiology GnRH Gonadotropin-Releasing Hormone - immunology Gonadotropin-Releasing Hormone - metabolism Male Marine Pituitary Pituitary Gland - metabolism Protein Isoforms - immunology Protein Isoforms - metabolism Scophthalmus maximus Seasons Turbot, Scophthalmus maximus |
| title | Three forms of GnRH in the brain and pituitary of the turbot, Scophthalmus maximus: immunological characterization and seasonal variation |
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