Antigenic comparison of a truncated form of VP2 of infectious pancreatic necrosis (IPN) virus expressed in four different cell types
A truncated form of the structural protein VP2 (truncVP2) of infectious pancreatic necrosis (IPN) virus encompassing amino acids 147–307 was expressed in bacterial, yeast, piscine and mammalian cells. All four recombinant antigens were recognised by a VP2-specific monoclonal antibody by ELISA and im...
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| Veröffentlicht in: | Fish & shellfish immunology 2001-04, Vol.11 (3), p.203-216 |
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| container_title | Fish & shellfish immunology |
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| creator | Labus, Marie B. Breeman, Suzanne Ellis, Anthony E. Smail, David A. Kervick, Mary Melvin, William T. |
| description | A truncated form of the structural protein VP2 (truncVP2) of infectious pancreatic necrosis (IPN) virus encompassing amino acids 147–307 was expressed in bacterial, yeast, piscine and mammalian cells. All four recombinant antigens were recognised by a VP2-specific monoclonal antibody by ELISA and immunoblot analysis. However, the minimum amount of r-truncVP2 needed for detection by these methods varies depending on the cell type used for expression. Furthermore, all four recombinant preparations, when used to immunise Atlantic salmon, were capable of inducing antibodies reactive with whole IPNV in ELISA. |
| doi_str_mv | 10.1006/fsim.2000.0308 |
| format | Article |
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All four recombinant antigens were recognised by a VP2-specific monoclonal antibody by ELISA and immunoblot analysis. However, the minimum amount of r-truncVP2 needed for detection by these methods varies depending on the cell type used for expression. Furthermore, all four recombinant preparations, when used to immunise Atlantic salmon, were capable of inducing antibodies reactive with whole IPNV in ELISA.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1006/fsim.2000.0308</identifier><identifier>PMID: 11394688</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Viral - blood ; Antigens, Viral - biosynthesis ; Antigens, Viral - genetics ; Antigens, Viral - immunology ; Base Sequence ; Birnaviridae Infections - immunology ; Birnaviridae Infections - prevention & control ; Blotting, Western - veterinary ; Brackish ; Capsid - genetics ; Capsid - immunology ; Capsid Proteins ; CHO Cells ; Cricetinae ; Enzyme-Linked Immunosorbent Assay - veterinary ; Epitopes - immunology ; Escherichia coli - genetics ; Freshwater ; Immunization - veterinary ; Infectious pancreatic necrosis virus ; Infectious pancreatic necrosis virus - genetics ; Infectious pancreatic necrosis virus - immunology ; infectious pancreatic necrosis virus, monoclonal antibody, recombinant protein, ELISA, immunoblot ; Marine ; Molecular Sequence Data ; Peptide Fragments - biosynthesis ; Peptide Fragments - genetics ; Peptide Fragments - immunology ; Pichia - genetics ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Salmo salar ; Salmon - immunology ; Viral Vaccines - immunology ; Viral Vaccines - standards ; VP2 protein</subject><ispartof>Fish & shellfish immunology, 2001-04, Vol.11 (3), p.203-216</ispartof><rights>2001 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-c8817c1eb8e87a092fff2fd5b91f76cd0bdae54934c5bbd6deb7a3763f18942d3</citedby><cites>FETCH-LOGICAL-c371t-c8817c1eb8e87a092fff2fd5b91f76cd0bdae54934c5bbd6deb7a3763f18942d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1050464800903085$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11394688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Labus, Marie B.</creatorcontrib><creatorcontrib>Breeman, Suzanne</creatorcontrib><creatorcontrib>Ellis, Anthony E.</creatorcontrib><creatorcontrib>Smail, David A.</creatorcontrib><creatorcontrib>Kervick, Mary</creatorcontrib><creatorcontrib>Melvin, William T.</creatorcontrib><title>Antigenic comparison of a truncated form of VP2 of infectious pancreatic necrosis (IPN) virus expressed in four different cell types</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>A truncated form of the structural protein VP2 (truncVP2) of infectious pancreatic necrosis (IPN) virus encompassing amino acids 147–307 was expressed in bacterial, yeast, piscine and mammalian cells. 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All four recombinant antigens were recognised by a VP2-specific monoclonal antibody by ELISA and immunoblot analysis. However, the minimum amount of r-truncVP2 needed for detection by these methods varies depending on the cell type used for expression. Furthermore, all four recombinant preparations, when used to immunise Atlantic salmon, were capable of inducing antibodies reactive with whole IPNV in ELISA.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>11394688</pmid><doi>10.1006/fsim.2000.0308</doi><tpages>14</tpages></addata></record> |
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| subjects | Animals Antibodies, Viral - blood Antigens, Viral - biosynthesis Antigens, Viral - genetics Antigens, Viral - immunology Base Sequence Birnaviridae Infections - immunology Birnaviridae Infections - prevention & control Blotting, Western - veterinary Brackish Capsid - genetics Capsid - immunology Capsid Proteins CHO Cells Cricetinae Enzyme-Linked Immunosorbent Assay - veterinary Epitopes - immunology Escherichia coli - genetics Freshwater Immunization - veterinary Infectious pancreatic necrosis virus Infectious pancreatic necrosis virus - genetics Infectious pancreatic necrosis virus - immunology infectious pancreatic necrosis virus, monoclonal antibody, recombinant protein, ELISA, immunoblot Marine Molecular Sequence Data Peptide Fragments - biosynthesis Peptide Fragments - genetics Peptide Fragments - immunology Pichia - genetics Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - immunology Salmo salar Salmon - immunology Viral Vaccines - immunology Viral Vaccines - standards VP2 protein |
| title | Antigenic comparison of a truncated form of VP2 of infectious pancreatic necrosis (IPN) virus expressed in four different cell types |
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