The Alpha-fetoprotein Promoter Is the Target of Afr1-Mediated Postnatal Repression
The α-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previou...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2000-01, Vol.63 (2), p.173-180 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Peyton, David K. Huang, Mei-Chuan Giglia, Meredith A. Hughes, Nedda K. Spear, Brett T. |
| description | The α-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15. |
| doi_str_mv | 10.1006/geno.1999.6073 |
| format | Article |
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This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1999.6073</identifier><identifier>PMID: 10673330</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>a-fetoprotein ; AFP gene ; Afr1 gene ; Alpha-fetoprotein regulator 1 protein ; alpha-Fetoproteins - genetics ; Animals ; Animals, Newborn ; Base Sequence ; Biological and medical sciences ; chromosome 15 ; Chromosome Mapping ; DNA Primers - genetics ; Enhancer Elements, Genetic ; Female ; Fetus - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Genes, Regulator ; Genes. Genome ; Humans ; Liver - metabolism ; Mice ; Mice, Inbred C3H ; Mice, Inbred C57BL ; Mice, Transgenic ; Molecular and cellular biology ; Molecular genetics ; Pregnancy ; Promoter Regions, Genetic ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; transgenes</subject><ispartof>Genomics (San Diego, Calif.), 2000-01, Vol.63 (2), p.173-180</ispartof><rights>2000 Academic Press</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-cdc0706f15f7668d50d362d3f4cb6d1b66d7a92f9da9f6cee0bba2d6bcce2d0d3</citedby><cites>FETCH-LOGICAL-c400t-cdc0706f15f7668d50d362d3f4cb6d1b66d7a92f9da9f6cee0bba2d6bcce2d0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/geno.1999.6073$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1290783$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10673330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peyton, David K.</creatorcontrib><creatorcontrib>Huang, Mei-Chuan</creatorcontrib><creatorcontrib>Giglia, Meredith A.</creatorcontrib><creatorcontrib>Hughes, Nedda K.</creatorcontrib><creatorcontrib>Spear, Brett T.</creatorcontrib><title>The Alpha-fetoprotein Promoter Is the Target of Afr1-Mediated Postnatal Repression</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>The α-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15.</description><subject>a-fetoprotein</subject><subject>AFP gene</subject><subject>Afr1 gene</subject><subject>Alpha-fetoprotein regulator 1 protein</subject><subject>alpha-Fetoproteins - genetics</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>chromosome 15</subject><subject>Chromosome Mapping</subject><subject>DNA Primers - genetics</subject><subject>Enhancer Elements, Genetic</subject><subject>Female</subject><subject>Fetus - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes, Regulator</subject><subject>Genes. Genome</subject><subject>Humans</subject><subject>Liver - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Pregnancy</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>transgenes</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1rGzEQgGERGmo36TXHsofS2zqjla2PozH9MDgkBOcstNIoUVivXEkO5N93FxuSS-hJOjwzDC8hVxRmFIBfP2IfZ1QpNeMg2BmZUpCqlnzOP5EpSClrsZizCfmS8zMAKCabz2RCgQvGGEzJ_fYJq2W3fzK1xxL3KRYMfXWX4m74pWqdqzKIrUmPWKroq6VPtL5BF0xBV93FXHpTTFfd4z5hziH2l-Tcmy7j19N7QR5-_dyu_tSb29_r1XJT2zlAqa2zIIB7uvCCc-kW4BhvHPNz23JHW86dMKrxyhnluUWEtjWN46212LgBX5Afx73D0X8PmIvehWyx60yP8ZC1AEUZE4v_QioUbSQb4ewIbYo5J_R6n8LOpFdNQY-59Zhbj7n1mHsY-HbafGh36N7xY98BfD8Bk63pfDK9DfnNNQqEHPfII8Oh10vApLMN2Nshc0JbtIvhoxP-AXtSnDM</recordid><startdate>20000115</startdate><enddate>20000115</enddate><creator>Peyton, David K.</creator><creator>Huang, Mei-Chuan</creator><creator>Giglia, Meredith A.</creator><creator>Hughes, Nedda K.</creator><creator>Spear, Brett T.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000115</creationdate><title>The Alpha-fetoprotein Promoter Is the Target of Afr1-Mediated Postnatal Repression</title><author>Peyton, David K. ; Huang, Mei-Chuan ; Giglia, Meredith A. ; Hughes, Nedda K. ; Spear, Brett T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-cdc0706f15f7668d50d362d3f4cb6d1b66d7a92f9da9f6cee0bba2d6bcce2d0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>a-fetoprotein</topic><topic>AFP gene</topic><topic>Afr1 gene</topic><topic>Alpha-fetoprotein regulator 1 protein</topic><topic>alpha-Fetoproteins - genetics</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>chromosome 15</topic><topic>Chromosome Mapping</topic><topic>DNA Primers - genetics</topic><topic>Enhancer Elements, Genetic</topic><topic>Female</topic><topic>Fetus - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes, Regulator</topic><topic>Genes. Genome</topic><topic>Humans</topic><topic>Liver - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Pregnancy</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>transgenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peyton, David K.</creatorcontrib><creatorcontrib>Huang, Mei-Chuan</creatorcontrib><creatorcontrib>Giglia, Meredith A.</creatorcontrib><creatorcontrib>Hughes, Nedda K.</creatorcontrib><creatorcontrib>Spear, Brett T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peyton, David K.</au><au>Huang, Mei-Chuan</au><au>Giglia, Meredith A.</au><au>Hughes, Nedda K.</au><au>Spear, Brett T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Alpha-fetoprotein Promoter Is the Target of Afr1-Mediated Postnatal Repression</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2000-01-15</date><risdate>2000</risdate><volume>63</volume><issue>2</issue><spage>173</spage><epage>180</epage><pages>173-180</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>The α-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>10673330</pmid><doi>10.1006/geno.1999.6073</doi><tpages>8</tpages></addata></record> |
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| subjects | a-fetoprotein AFP gene Afr1 gene Alpha-fetoprotein regulator 1 protein alpha-Fetoproteins - genetics Animals Animals, Newborn Base Sequence Biological and medical sciences chromosome 15 Chromosome Mapping DNA Primers - genetics Enhancer Elements, Genetic Female Fetus - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Genes, Regulator Genes. Genome Humans Liver - metabolism Mice Mice, Inbred C3H Mice, Inbred C57BL Mice, Transgenic Molecular and cellular biology Molecular genetics Pregnancy Promoter Regions, Genetic RNA, Messenger - genetics RNA, Messenger - metabolism transgenes |
| title | The Alpha-fetoprotein Promoter Is the Target of Afr1-Mediated Postnatal Repression |
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