Cloning and sequence analysis of the endopolygalacturonase gene from the pitch canker fungus, Fusarium circinatum
The fungus Fusarium circinatum causes pitch canker disease on mature pine trees and root rot and damping-off of pine seedlings. Endopolygalacturonases (endo-PGs) play a major role during penetration of plants by fungi. Digestion of the pectic polysaccharides in the plant primary cell walls is one of...
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| Veröffentlicht in: | Current microbiology 2001-05, Vol.42 (5), p.350-352 |
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| creator | CHIMWAMUROMBE, Percy M WINGFIELD, Brenda D BOTHA, Anna-Maria WINGFIELD, Michael J |
| description | The fungus Fusarium circinatum causes pitch canker disease on mature pine trees and root rot and damping-off of pine seedlings. Endopolygalacturonases (endo-PGs) play a major role during penetration of plants by fungi. Digestion of the pectic polysaccharides in the plant primary cell walls is one of the earliest functions of endoPGs during infection. The research objective was to clone and characterize the gene encoding endopolygalacturonase in F. circinatum. A 970-bp DNA fragment was cloned by using degenerate PCR amplification from F. circinatum DNA. Sequence data for this fragment were used to design specific primers for use in genome walking to amplify and sequence the remaining portion of the F. circinatum endoPG gene (Fcpg). The amino acid sequence predicted from this gene showed 90% and 87% similarity to Fusarium oxysporum and Fusarium moniliforme endoPGs, respectively. |
| doi_str_mv | 10.1007/s002840010228 |
| format | Article |
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Endopolygalacturonases (endo-PGs) play a major role during penetration of plants by fungi. Digestion of the pectic polysaccharides in the plant primary cell walls is one of the earliest functions of endoPGs during infection. The research objective was to clone and characterize the gene encoding endopolygalacturonase in F. circinatum. A 970-bp DNA fragment was cloned by using degenerate PCR amplification from F. circinatum DNA. Sequence data for this fragment were used to design specific primers for use in genome walking to amplify and sequence the remaining portion of the F. circinatum endoPG gene (Fcpg). The amino acid sequence predicted from this gene showed 90% and 87% similarity to Fusarium oxysporum and Fusarium moniliforme endoPGs, respectively.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002840010228</identifier><identifier>PMID: 11400056</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Amino acid sequence ; Amino acids ; Biological and medical sciences ; Chromosome Walking ; Cloning ; Cloning, Molecular ; Deoxyribonucleic acid ; DNA ; Fundamental and applied biological sciences. Psychology ; Fusarium - enzymology ; Fusarium - genetics ; Fusarium circinatum ; Genes ; Genome, Fungal ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Microbiology ; Molecular Sequence Data ; Mycology ; Pine trees ; Plant Diseases - microbiology ; Polygalacturonase - genetics ; Polygalacturonase - metabolism ; Polymerase Chain Reaction - methods ; Saccharides ; Seedlings ; Sequence Analysis, DNA ; Trees - microbiology</subject><ispartof>Current microbiology, 2001-05, Vol.42 (5), p.350-352</ispartof><rights>Springer-Verlag New York Inc. 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-686b5266e898677a624b1fc54b7b0ac9fd7b04803cac1047f4cb11c2adc5d67e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14232638$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11400056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHIMWAMUROMBE, Percy M</creatorcontrib><creatorcontrib>WINGFIELD, Brenda D</creatorcontrib><creatorcontrib>BOTHA, Anna-Maria</creatorcontrib><creatorcontrib>WINGFIELD, Michael J</creatorcontrib><title>Cloning and sequence analysis of the endopolygalacturonase gene from the pitch canker fungus, Fusarium circinatum</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>The fungus Fusarium circinatum causes pitch canker disease on mature pine trees and root rot and damping-off of pine seedlings. Endopolygalacturonases (endo-PGs) play a major role during penetration of plants by fungi. Digestion of the pectic polysaccharides in the plant primary cell walls is one of the earliest functions of endoPGs during infection. The research objective was to clone and characterize the gene encoding endopolygalacturonase in F. circinatum. A 970-bp DNA fragment was cloned by using degenerate PCR amplification from F. circinatum DNA. Sequence data for this fragment were used to design specific primers for use in genome walking to amplify and sequence the remaining portion of the F. circinatum endoPG gene (Fcpg). The amino acid sequence predicted from this gene showed 90% and 87% similarity to Fusarium oxysporum and Fusarium moniliforme endoPGs, respectively.</description><subject>Amino acid sequence</subject><subject>Amino acids</subject><subject>Biological and medical sciences</subject><subject>Chromosome Walking</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fusarium - enzymology</subject><subject>Fusarium - genetics</subject><subject>Fusarium circinatum</subject><subject>Genes</subject><subject>Genome, Fungal</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mycology</subject><subject>Pine trees</subject><subject>Plant Diseases - microbiology</subject><subject>Polygalacturonase - genetics</subject><subject>Polygalacturonase - metabolism</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Saccharides</subject><subject>Seedlings</subject><subject>Sequence Analysis, DNA</subject><subject>Trees - microbiology</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0c-L1DAUB_AgijuuHr1qQFw8WH350TQ9yrCrwoIH3XN5TZNu1zaZTZrD_PdmnIFFD3p6BD75hrwvIS8ZfGAAzccEwLUEYMC5fkQ2TApeQduyx2QDQopKq5qdkWcp3RXEW2BPyRlj5QbUakPut3Pwkx8p-oEme5-tN7YccN6nKdHg6HprqfVD2IV5P-KMZs0xeEyWjtZb6mJYfpvdtJpbatD_tJG67Mec3tOrnDBOeaFmimbyuOblOXnicE72xWmek5uryx_bL9X1t89ft5-uKyNFvVZKq77mSlndatU0qLjsmTO17Jse0LRuKFNqEAYNA9k4aXrGDMfB1INqrDgnF8fcXQzlW2ntlikZO8_obcipa6A9rAP-C5kWqgYuCnz3byil5lzWwAp98xe9CzmWrR6UYG0jhVRFVUdlYkgpWtft4rRg3HcMukO73R_tFv_qlJr7xQ4P-lRnAW9PAJPB2UX0ZkoPTnLBlTgEvT46h6HDMRZz850DU-UlKVvWil9f3bTr</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>CHIMWAMUROMBE, Percy M</creator><creator>WINGFIELD, Brenda D</creator><creator>BOTHA, Anna-Maria</creator><creator>WINGFIELD, Michael J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Cloning and sequence analysis of the endopolygalacturonase gene from the pitch canker fungus, Fusarium circinatum</title><author>CHIMWAMUROMBE, Percy M ; WINGFIELD, Brenda D ; BOTHA, Anna-Maria ; WINGFIELD, Michael J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-686b5266e898677a624b1fc54b7b0ac9fd7b04803cac1047f4cb11c2adc5d67e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino acid sequence</topic><topic>Amino acids</topic><topic>Biological and medical sciences</topic><topic>Chromosome Walking</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fusarium - enzymology</topic><topic>Fusarium - genetics</topic><topic>Fusarium circinatum</topic><topic>Genes</topic><topic>Genome, Fungal</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mycology</topic><topic>Pine trees</topic><topic>Plant Diseases - microbiology</topic><topic>Polygalacturonase - genetics</topic><topic>Polygalacturonase - metabolism</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Saccharides</topic><topic>Seedlings</topic><topic>Sequence Analysis, DNA</topic><topic>Trees - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHIMWAMUROMBE, Percy M</creatorcontrib><creatorcontrib>WINGFIELD, Brenda D</creatorcontrib><creatorcontrib>BOTHA, Anna-Maria</creatorcontrib><creatorcontrib>WINGFIELD, Michael J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHIMWAMUROMBE, Percy M</au><au>WINGFIELD, Brenda D</au><au>BOTHA, Anna-Maria</au><au>WINGFIELD, Michael J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and sequence analysis of the endopolygalacturonase gene from the pitch canker fungus, Fusarium circinatum</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>42</volume><issue>5</issue><spage>350</spage><epage>352</epage><pages>350-352</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>The fungus Fusarium circinatum causes pitch canker disease on mature pine trees and root rot and damping-off of pine seedlings. Endopolygalacturonases (endo-PGs) play a major role during penetration of plants by fungi. Digestion of the pectic polysaccharides in the plant primary cell walls is one of the earliest functions of endoPGs during infection. The research objective was to clone and characterize the gene encoding endopolygalacturonase in F. circinatum. A 970-bp DNA fragment was cloned by using degenerate PCR amplification from F. circinatum DNA. Sequence data for this fragment were used to design specific primers for use in genome walking to amplify and sequence the remaining portion of the F. circinatum endoPG gene (Fcpg). The amino acid sequence predicted from this gene showed 90% and 87% similarity to Fusarium oxysporum and Fusarium moniliforme endoPGs, respectively.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>11400056</pmid><doi>10.1007/s002840010228</doi><tpages>3</tpages></addata></record> |
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| ispartof | Current microbiology, 2001-05, Vol.42 (5), p.350-352 |
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| subjects | Amino acid sequence Amino acids Biological and medical sciences Chromosome Walking Cloning Cloning, Molecular Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Fusarium - enzymology Fusarium - genetics Fusarium circinatum Genes Genome, Fungal Growth, nutrition, metabolism, transports, enzymes. Molecular biology Microbiology Molecular Sequence Data Mycology Pine trees Plant Diseases - microbiology Polygalacturonase - genetics Polygalacturonase - metabolism Polymerase Chain Reaction - methods Saccharides Seedlings Sequence Analysis, DNA Trees - microbiology |
| title | Cloning and sequence analysis of the endopolygalacturonase gene from the pitch canker fungus, Fusarium circinatum |
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