Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen
Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens repor...
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Veröffentlicht in: | Journal of autoimmunity 2000-03, Vol.14 (2), p.179-187 |
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creator | Eystathioy, Theophany Jakymiw, Andrew Fujita, Donald J Fritzler, Marvin J Chan, Edward K.L |
description | Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67kDa which has four regions with sequence identity to gm130, ranging between 42 and 60%. In this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in α-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed. |
doi_str_mv | 10.1006/jaut.1999.0359 |
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We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67kDa which has four regions with sequence identity to gm130, ranging between 42 and 60%. In this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in α-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed.</description><identifier>ISSN: 0896-8411</identifier><identifier>EISSN: 1095-9157</identifier><identifier>DOI: 10.1006/jaut.1999.0359</identifier><identifier>PMID: 10677249</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Autoantibodies - blood ; autoantibody, autoantigen, autoimmunity, golgi complex ; Autoantigens - chemistry ; Autoantigens - genetics ; Autoantigens - immunology ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; DNA Primers - genetics ; DNA, Complementary - genetics ; General aspects ; Golgi Apparatus - immunology ; golgin-67 ; Humans ; Immunopathology ; Medical sciences ; Membrane Proteins - chemistry ; Membrane Proteins - genetics ; Membrane Proteins - immunology ; Molecular Sequence Data ; Protein Structure, Tertiary ; Proteins ; Rabbits ; Sequence Homology, Amino Acid</subject><ispartof>Journal of autoimmunity, 2000-03, Vol.14 (2), p.179-187</ispartof><rights>2000 Academic Press</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-c742607221735d7b8c908759379788c9351474bc7784320bda34e6f4edf8cb083</citedby><cites>FETCH-LOGICAL-c429t-c742607221735d7b8c908759379788c9351474bc7784320bda34e6f4edf8cb083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0896841199903591$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1363874$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10677249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eystathioy, Theophany</creatorcontrib><creatorcontrib>Jakymiw, Andrew</creatorcontrib><creatorcontrib>Fujita, Donald J</creatorcontrib><creatorcontrib>Fritzler, Marvin J</creatorcontrib><creatorcontrib>Chan, Edward K.L</creatorcontrib><title>Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen</title><title>Journal of autoimmunity</title><addtitle>J Autoimmun</addtitle><description>Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67kDa which has four regions with sequence identity to gm130, ranging between 42 and 60%. In this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in α-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Autoantibodies - blood</subject><subject>autoantibody, autoantigen, autoimmunity, golgi complex</subject><subject>Autoantigens - chemistry</subject><subject>Autoantigens - genetics</subject><subject>Autoantigens - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA Primers - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>General aspects</subject><subject>Golgi Apparatus - immunology</subject><subject>golgin-67</subject><subject>Humans</subject><subject>Immunopathology</subject><subject>Medical sciences</subject><subject>Membrane Proteins - chemistry</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - immunology</subject><subject>Molecular Sequence Data</subject><subject>Protein Structure, Tertiary</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Sequence Homology, Amino Acid</subject><issn>0896-8411</issn><issn>1095-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EokvhyhH5gLhl64ntjM2tqqCLVAESII6W4zhbr5K42E6l_vdkleXjgjiNnuY3T6P3CHkJbAuMNRcHO5ctaK23jEv9iGyAaVlpkPiYbJjSTaUEwBl5lvOBMQAp5VNyBqxBrIXekMNuHu1EL-cS7VRCG7vgMy2RWvox3vuBXsdhH-jnFIsP06qmqsG3dBf2t_RLGMNgUygP9Hsot7_2Wl7sRwqc_Tbe--k5edLbIfsXp3lOvr1_9_VqV918uv5wdXlTOVHrUjkUdcOwrgG57LBVTjOFUnPUqBbBJQgUrUNUgtes7SwXvumF73rlWqb4OXmz-t6l-GP2uZgxZOeHwU4-ztkg07AkVv8XBNRQayYXcLuCLsWck-_NXQqjTQ8GmDnWYI41mGMN5ljDcvDq5Dy3o-_-wtfcF-D1CbDZ2aFPdnIh_-F4wxWKBVMr5pe87oNPJrvgJ-e7kLwrpovhXy_8BAOKoM4</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Eystathioy, Theophany</creator><creator>Jakymiw, Andrew</creator><creator>Fujita, Donald J</creator><creator>Fritzler, Marvin J</creator><creator>Chan, Edward K.L</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen</title><author>Eystathioy, Theophany ; Jakymiw, Andrew ; Fujita, Donald J ; Fritzler, Marvin J ; Chan, Edward K.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-c742607221735d7b8c908759379788c9351474bc7784320bda34e6f4edf8cb083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Autoantibodies - blood</topic><topic>autoantibody, autoantigen, autoimmunity, golgi complex</topic><topic>Autoantigens - chemistry</topic><topic>Autoantigens - genetics</topic><topic>Autoantigens - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA Primers - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>General aspects</topic><topic>Golgi Apparatus - immunology</topic><topic>golgin-67</topic><topic>Humans</topic><topic>Immunopathology</topic><topic>Medical sciences</topic><topic>Membrane Proteins - chemistry</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - immunology</topic><topic>Molecular Sequence Data</topic><topic>Protein Structure, Tertiary</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eystathioy, Theophany</creatorcontrib><creatorcontrib>Jakymiw, Andrew</creatorcontrib><creatorcontrib>Fujita, Donald J</creatorcontrib><creatorcontrib>Fritzler, Marvin J</creatorcontrib><creatorcontrib>Chan, Edward K.L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of autoimmunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eystathioy, Theophany</au><au>Jakymiw, Andrew</au><au>Fujita, Donald J</au><au>Fritzler, Marvin J</au><au>Chan, Edward K.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen</atitle><jtitle>Journal of autoimmunity</jtitle><addtitle>J Autoimmun</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>14</volume><issue>2</issue><spage>179</spage><epage>187</epage><pages>179-187</pages><issn>0896-8411</issn><eissn>1095-9157</eissn><abstract>Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67kDa which has four regions with sequence identity to gm130, ranging between 42 and 60%. In this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in α-helical coiled-coil domain. 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subjects | Amino Acid Sequence Animals Autoantibodies - blood autoantibody, autoantigen, autoimmunity, golgi complex Autoantigens - chemistry Autoantigens - genetics Autoantigens - immunology Base Sequence Biological and medical sciences Cloning, Molecular DNA Primers - genetics DNA, Complementary - genetics General aspects Golgi Apparatus - immunology golgin-67 Humans Immunopathology Medical sciences Membrane Proteins - chemistry Membrane Proteins - genetics Membrane Proteins - immunology Molecular Sequence Data Protein Structure, Tertiary Proteins Rabbits Sequence Homology, Amino Acid |
title | Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen |
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