Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen

Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens repor...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of autoimmunity 2000-03, Vol.14 (2), p.179-187
Hauptverfasser: Eystathioy, Theophany, Jakymiw, Andrew, Fujita, Donald J, Fritzler, Marvin J, Chan, Edward K.L
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 187
container_issue 2
container_start_page 179
container_title Journal of autoimmunity
container_volume 14
creator Eystathioy, Theophany
Jakymiw, Andrew
Fujita, Donald J
Fritzler, Marvin J
Chan, Edward K.L
description Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67kDa which has four regions with sequence identity to gm130, ranging between 42 and 60%. In this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in α-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed.
doi_str_mv 10.1006/jaut.1999.0359
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70911992</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0896841199903591</els_id><sourcerecordid>70911992</sourcerecordid><originalsourceid>FETCH-LOGICAL-c429t-c742607221735d7b8c908759379788c9351474bc7784320bda34e6f4edf8cb083</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS0EokvhyhH5gLhl64ntjM2tqqCLVAESII6W4zhbr5K42E6l_vdkleXjgjiNnuY3T6P3CHkJbAuMNRcHO5ctaK23jEv9iGyAaVlpkPiYbJjSTaUEwBl5lvOBMQAp5VNyBqxBrIXekMNuHu1EL-cS7VRCG7vgMy2RWvox3vuBXsdhH-jnFIsP06qmqsG3dBf2t_RLGMNgUygP9Hsot7_2Wl7sRwqc_Tbe--k5edLbIfsXp3lOvr1_9_VqV918uv5wdXlTOVHrUjkUdcOwrgG57LBVTjOFUnPUqBbBJQgUrUNUgtes7SwXvumF73rlWqb4OXmz-t6l-GP2uZgxZOeHwU4-ztkg07AkVv8XBNRQayYXcLuCLsWck-_NXQqjTQ8GmDnWYI41mGMN5ljDcvDq5Dy3o-_-wtfcF-D1CbDZ2aFPdnIh_-F4wxWKBVMr5pe87oNPJrvgJ-e7kLwrpovhXy_8BAOKoM4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17912905</pqid></control><display><type>article</type><title>Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Eystathioy, Theophany ; Jakymiw, Andrew ; Fujita, Donald J ; Fritzler, Marvin J ; Chan, Edward K.L</creator><creatorcontrib>Eystathioy, Theophany ; Jakymiw, Andrew ; Fujita, Donald J ; Fritzler, Marvin J ; Chan, Edward K.L</creatorcontrib><description>Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67kDa which has four regions with sequence identity to gm130, ranging between 42 and 60%. In this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in α-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed.</description><identifier>ISSN: 0896-8411</identifier><identifier>EISSN: 1095-9157</identifier><identifier>DOI: 10.1006/jaut.1999.0359</identifier><identifier>PMID: 10677249</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Autoantibodies - blood ; autoantibody, autoantigen, autoimmunity, golgi complex ; Autoantigens - chemistry ; Autoantigens - genetics ; Autoantigens - immunology ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; DNA Primers - genetics ; DNA, Complementary - genetics ; General aspects ; Golgi Apparatus - immunology ; golgin-67 ; Humans ; Immunopathology ; Medical sciences ; Membrane Proteins - chemistry ; Membrane Proteins - genetics ; Membrane Proteins - immunology ; Molecular Sequence Data ; Protein Structure, Tertiary ; Proteins ; Rabbits ; Sequence Homology, Amino Acid</subject><ispartof>Journal of autoimmunity, 2000-03, Vol.14 (2), p.179-187</ispartof><rights>2000 Academic Press</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-c742607221735d7b8c908759379788c9351474bc7784320bda34e6f4edf8cb083</citedby><cites>FETCH-LOGICAL-c429t-c742607221735d7b8c908759379788c9351474bc7784320bda34e6f4edf8cb083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0896841199903591$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1363874$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10677249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eystathioy, Theophany</creatorcontrib><creatorcontrib>Jakymiw, Andrew</creatorcontrib><creatorcontrib>Fujita, Donald J</creatorcontrib><creatorcontrib>Fritzler, Marvin J</creatorcontrib><creatorcontrib>Chan, Edward K.L</creatorcontrib><title>Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen</title><title>Journal of autoimmunity</title><addtitle>J Autoimmun</addtitle><description>Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67kDa which has four regions with sequence identity to gm130, ranging between 42 and 60%. In this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in α-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Autoantibodies - blood</subject><subject>autoantibody, autoantigen, autoimmunity, golgi complex</subject><subject>Autoantigens - chemistry</subject><subject>Autoantigens - genetics</subject><subject>Autoantigens - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA Primers - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>General aspects</subject><subject>Golgi Apparatus - immunology</subject><subject>golgin-67</subject><subject>Humans</subject><subject>Immunopathology</subject><subject>Medical sciences</subject><subject>Membrane Proteins - chemistry</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - immunology</subject><subject>Molecular Sequence Data</subject><subject>Protein Structure, Tertiary</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Sequence Homology, Amino Acid</subject><issn>0896-8411</issn><issn>1095-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EokvhyhH5gLhl64ntjM2tqqCLVAESII6W4zhbr5K42E6l_vdkleXjgjiNnuY3T6P3CHkJbAuMNRcHO5ctaK23jEv9iGyAaVlpkPiYbJjSTaUEwBl5lvOBMQAp5VNyBqxBrIXekMNuHu1EL-cS7VRCG7vgMy2RWvox3vuBXsdhH-jnFIsP06qmqsG3dBf2t_RLGMNgUygP9Hsot7_2Wl7sRwqc_Tbe--k5edLbIfsXp3lOvr1_9_VqV918uv5wdXlTOVHrUjkUdcOwrgG57LBVTjOFUnPUqBbBJQgUrUNUgtes7SwXvumF73rlWqb4OXmz-t6l-GP2uZgxZOeHwU4-ztkg07AkVv8XBNRQayYXcLuCLsWck-_NXQqjTQ8GmDnWYI41mGMN5ljDcvDq5Dy3o-_-wtfcF-D1CbDZ2aFPdnIh_-F4wxWKBVMr5pe87oNPJrvgJ-e7kLwrpovhXy_8BAOKoM4</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Eystathioy, Theophany</creator><creator>Jakymiw, Andrew</creator><creator>Fujita, Donald J</creator><creator>Fritzler, Marvin J</creator><creator>Chan, Edward K.L</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen</title><author>Eystathioy, Theophany ; Jakymiw, Andrew ; Fujita, Donald J ; Fritzler, Marvin J ; Chan, Edward K.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-c742607221735d7b8c908759379788c9351474bc7784320bda34e6f4edf8cb083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Autoantibodies - blood</topic><topic>autoantibody, autoantigen, autoimmunity, golgi complex</topic><topic>Autoantigens - chemistry</topic><topic>Autoantigens - genetics</topic><topic>Autoantigens - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA Primers - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>General aspects</topic><topic>Golgi Apparatus - immunology</topic><topic>golgin-67</topic><topic>Humans</topic><topic>Immunopathology</topic><topic>Medical sciences</topic><topic>Membrane Proteins - chemistry</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - immunology</topic><topic>Molecular Sequence Data</topic><topic>Protein Structure, Tertiary</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eystathioy, Theophany</creatorcontrib><creatorcontrib>Jakymiw, Andrew</creatorcontrib><creatorcontrib>Fujita, Donald J</creatorcontrib><creatorcontrib>Fritzler, Marvin J</creatorcontrib><creatorcontrib>Chan, Edward K.L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of autoimmunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eystathioy, Theophany</au><au>Jakymiw, Andrew</au><au>Fujita, Donald J</au><au>Fritzler, Marvin J</au><au>Chan, Edward K.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen</atitle><jtitle>Journal of autoimmunity</jtitle><addtitle>J Autoimmun</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>14</volume><issue>2</issue><spage>179</spage><epage>187</epage><pages>179-187</pages><issn>0896-8411</issn><eissn>1095-9157</eissn><abstract>Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67kDa which has four regions with sequence identity to gm130, ranging between 42 and 60%. In this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in α-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>10677249</pmid><doi>10.1006/jaut.1999.0359</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0896-8411
ispartof Journal of autoimmunity, 2000-03, Vol.14 (2), p.179-187
issn 0896-8411
1095-9157
language eng
recordid cdi_proquest_miscellaneous_70911992
source MEDLINE; Elsevier ScienceDirect Journals
subjects Amino Acid Sequence
Animals
Autoantibodies - blood
autoantibody, autoantigen, autoimmunity, golgi complex
Autoantigens - chemistry
Autoantigens - genetics
Autoantigens - immunology
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA Primers - genetics
DNA, Complementary - genetics
General aspects
Golgi Apparatus - immunology
golgin-67
Humans
Immunopathology
Medical sciences
Membrane Proteins - chemistry
Membrane Proteins - genetics
Membrane Proteins - immunology
Molecular Sequence Data
Protein Structure, Tertiary
Proteins
Rabbits
Sequence Homology, Amino Acid
title Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T10%3A23%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20Autoantibodies%20to%20a%20Novel%20Golgi%20Protein%20Golgin-67:%20High%20Similarity%20With%20Golgin-95/gm%20130%20Autoantigen&rft.jtitle=Journal%20of%20autoimmunity&rft.au=Eystathioy,%20Theophany&rft.date=2000-03-01&rft.volume=14&rft.issue=2&rft.spage=179&rft.epage=187&rft.pages=179-187&rft.issn=0896-8411&rft.eissn=1095-9157&rft_id=info:doi/10.1006/jaut.1999.0359&rft_dat=%3Cproquest_cross%3E70911992%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17912905&rft_id=info:pmid/10677249&rft_els_id=S0896841199903591&rfr_iscdi=true