Age-Related Decline in Osteoprotegerin Expression by Human Bone Marrow Cells Cultured in Three-Dimensional Collagen Sponges
With advancing age, an increase in bone resorption relative to bone formation results in bone loss. Bone marrow stromal cells and their products support osteoclastogenesis from hematopoietic progenitors. Another of their products, osteoprotegerin (OPG), blocks the osteoclast–stimulatory effects of O...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-02, Vol.268 (3), p.669-672 |
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| creator | Makhluf, Huda A. Mueller, Stefan M. Mizuno, Shuichi Glowacki, Julie |
| description | With advancing age, an increase in bone resorption relative to bone formation results in bone loss. Bone marrow stromal cells and their products support osteoclastogenesis from hematopoietic progenitors. Another of their products, osteoprotegerin (OPG), blocks the osteoclast–stimulatory effects of OPG ligand. We tested the hypothesis that with advancing age there is a decrease in OPG expression by human bone marrow cells. Bone marrow cells were obtained from 18 subjects (age range 38–84 years). Expression of mRNA transcripts of OPG was assessed by quantitative competitive RT-PCR. Median number of OPG transcripts in the younger group was 0.3 zetptomoles (range 0.01 to 1.30) and was higher than in the older group's median of 0.06 (range 0 to 0.5; p < 0.05). The decline in the expression of OPG with age may increase the capacity of stromal/osteoblast cells to support osteoclastogenesis. |
| doi_str_mv | 10.1006/bbrc.2000.2182 |
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Bone marrow stromal cells and their products support osteoclastogenesis from hematopoietic progenitors. Another of their products, osteoprotegerin (OPG), blocks the osteoclast–stimulatory effects of OPG ligand. We tested the hypothesis that with advancing age there is a decrease in OPG expression by human bone marrow cells. Bone marrow cells were obtained from 18 subjects (age range 38–84 years). Expression of mRNA transcripts of OPG was assessed by quantitative competitive RT-PCR. Median number of OPG transcripts in the younger group was 0.3 zetptomoles (range 0.01 to 1.30) and was higher than in the older group's median of 0.06 (range 0 to 0.5; p < 0.05). 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Bone marrow stromal cells and their products support osteoclastogenesis from hematopoietic progenitors. Another of their products, osteoprotegerin (OPG), blocks the osteoclast–stimulatory effects of OPG ligand. We tested the hypothesis that with advancing age there is a decrease in OPG expression by human bone marrow cells. Bone marrow cells were obtained from 18 subjects (age range 38–84 years). Expression of mRNA transcripts of OPG was assessed by quantitative competitive RT-PCR. Median number of OPG transcripts in the younger group was 0.3 zetptomoles (range 0.01 to 1.30) and was higher than in the older group's median of 0.06 (range 0 to 0.5; p < 0.05). The decline in the expression of OPG with age may increase the capacity of stromal/osteoblast cells to support osteoclastogenesis.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Aging - genetics</subject><subject>Base Sequence</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Bone Resorption - genetics</subject><subject>Bone Resorption - metabolism</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>DNA Primers - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Glycoproteins - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Osteoclasts - metabolism</subject><subject>Osteoporosis - etiology</subject><subject>Osteoporosis - genetics</subject><subject>Osteoporosis - metabolism</subject><subject>Osteoprotegerin</subject><subject>Receptors, Cytoplasmic and Nuclear</subject><subject>Receptors, Tumor Necrosis Factor</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EotvClSPyiVuWsR3F8bGkhSK1qkRbiZvl2JPFKLEXO2mp-PM42h64oJ6ssb73NPMeIe8YbBlA87Hvk91yANhy1vIXZMNAQcUZ1C_Jpnw3FVfs-xE5zvknAGN1o16TIwaNVLzhG_LndIfVNxzNjI6eoR19QOoDvc4zxn2KM-4wlfn89z5hzj4G2j_Si2UygX6Khb0yKcUH2uE4Ztot47yk4lQUtz8SYnXmJwyrzIy0i-NodhjozT6GHeY35NVgxoxvn94Tcvf5_La7qC6vv3ztTi8rKySbKyFbEMARTO9qcE0rlRi4YI6r3ohB1gqVZFJC2yvVCzmAY0MNUjChrDBOnJAPB99yz68F86wnn21Z2ASMS9YSFIOSzLMgk6pEKlZwewBtijknHPQ--cmkR81Ar73otRe99qLXXorg_ZPz0k_o_sEPRRSgPQBYgrj3mHS2HoNF5xPaWbvo_-f9F-BInCA</recordid><startdate>20000224</startdate><enddate>20000224</enddate><creator>Makhluf, Huda A.</creator><creator>Mueller, Stefan M.</creator><creator>Mizuno, Shuichi</creator><creator>Glowacki, Julie</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>20000224</creationdate><title>Age-Related Decline in Osteoprotegerin Expression by Human Bone Marrow Cells Cultured in Three-Dimensional Collagen Sponges</title><author>Makhluf, Huda A. ; Mueller, Stefan M. ; Mizuno, Shuichi ; Glowacki, Julie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-3780302e0abd40d68793f231d29ba3f749e9717708b99b37f0d1f4073139c3ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Aging - genetics</topic><topic>Base Sequence</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Bone Resorption - genetics</topic><topic>Bone Resorption - metabolism</topic><topic>Cells, Cultured</topic><topic>Collagen</topic><topic>DNA Primers - genetics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Glycoproteins - genetics</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Osteoclasts - metabolism</topic><topic>Osteoporosis - etiology</topic><topic>Osteoporosis - genetics</topic><topic>Osteoporosis - metabolism</topic><topic>Osteoprotegerin</topic><topic>Receptors, Cytoplasmic and Nuclear</topic><topic>Receptors, Tumor Necrosis Factor</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Makhluf, Huda A.</creatorcontrib><creatorcontrib>Mueller, Stefan M.</creatorcontrib><creatorcontrib>Mizuno, Shuichi</creatorcontrib><creatorcontrib>Glowacki, Julie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Makhluf, Huda A.</au><au>Mueller, Stefan M.</au><au>Mizuno, Shuichi</au><au>Glowacki, Julie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-Related Decline in Osteoprotegerin Expression by Human Bone Marrow Cells Cultured in Three-Dimensional Collagen Sponges</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2000-02-24</date><risdate>2000</risdate><volume>268</volume><issue>3</issue><spage>669</spage><epage>672</epage><pages>669-672</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>With advancing age, an increase in bone resorption relative to bone formation results in bone loss. Bone marrow stromal cells and their products support osteoclastogenesis from hematopoietic progenitors. Another of their products, osteoprotegerin (OPG), blocks the osteoclast–stimulatory effects of OPG ligand. We tested the hypothesis that with advancing age there is a decrease in OPG expression by human bone marrow cells. Bone marrow cells were obtained from 18 subjects (age range 38–84 years). Expression of mRNA transcripts of OPG was assessed by quantitative competitive RT-PCR. Median number of OPG transcripts in the younger group was 0.3 zetptomoles (range 0.01 to 1.30) and was higher than in the older group's median of 0.06 (range 0 to 0.5; p < 0.05). The decline in the expression of OPG with age may increase the capacity of stromal/osteoblast cells to support osteoclastogenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10679262</pmid><doi>10.1006/bbrc.2000.2182</doi><tpages>4</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Aging - genetics Base Sequence Bone Marrow Cells - metabolism Bone Resorption - genetics Bone Resorption - metabolism Cells, Cultured Collagen DNA Primers - genetics Female Gene Expression Glycoproteins - genetics Humans Male Middle Aged Osteoclasts - metabolism Osteoporosis - etiology Osteoporosis - genetics Osteoporosis - metabolism Osteoprotegerin Receptors, Cytoplasmic and Nuclear Receptors, Tumor Necrosis Factor Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Age-Related Decline in Osteoprotegerin Expression by Human Bone Marrow Cells Cultured in Three-Dimensional Collagen Sponges |
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