Mechanical properties and structure of carotid arteries in mice lacking desmin
Our aim was to determine in desmin homozygous mutant mice the viscoelastic properties, the mechanical strength and the structure of the carotid artery. To assess the viscoelastic properties of large arteries, we have performed an in vivo analysis of the diameter-, and distensibility-pressure curves...
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| Veröffentlicht in: | Cardiovascular research 2001-07, Vol.51 (1), p.178-187 |
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| creator | LACOLLEY, Patrick CHALLANDE, Pascal BOUMAZA, Saliha COHUET, Géraldine LAURENT, Stéphane BOUTOUYRIE, Pierre GRIMAUD, Jean-Alexis PAULIN, Denise LAMAZIERE, Jean-Marie Daniel ZHENLIN LID |
| description | Our aim was to determine in desmin homozygous mutant mice the viscoelastic properties, the mechanical strength and the structure of the carotid artery.
To assess the viscoelastic properties of large arteries, we have performed an in vivo analysis of the diameter-, and distensibility-pressure curves of the common carotid artery (CCA) in homozygous (Des -/-), heterozygous (Des +/-) and wild-type (Des +/+) mice. To evaluate the mechanical strength, we have measured the in vitro intraluminal pressure producing the rupture of the carotid artery wall. The structure analysis of the arterial wall was based on histology and electronic microscopy.
A lower distensibility and an increase of arterial wall viscosity were observed in Des -/- compared with Des +/+. Arterial thickness of Des -/- was similar to those of Des +/+, without changes in elastin and collagen contents. Electron microscopy revealed that the perimeter of cellular fingerlike-projections was smaller in Des -/-, indicating that the cells have lost part of their connections to the extracellular matrix. The rupture pressure was significantly lower in Des -/- (1500+/-200 mmHg) compared with Des +/+ (2100+/-80 mmHg) indicating a lower mechanical strength of the vascular wall. No significant difference was found between Des +/- and Des +/+.
The desmin is essential to maintain proper viscoelastic properties, structure and mechanical strength of the vascular wall. |
| doi_str_mv | 10.1016/S0008-6363(01)00278-4 |
| format | Article |
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To assess the viscoelastic properties of large arteries, we have performed an in vivo analysis of the diameter-, and distensibility-pressure curves of the common carotid artery (CCA) in homozygous (Des -/-), heterozygous (Des +/-) and wild-type (Des +/+) mice. To evaluate the mechanical strength, we have measured the in vitro intraluminal pressure producing the rupture of the carotid artery wall. The structure analysis of the arterial wall was based on histology and electronic microscopy.
A lower distensibility and an increase of arterial wall viscosity were observed in Des -/- compared with Des +/+. Arterial thickness of Des -/- was similar to those of Des +/+, without changes in elastin and collagen contents. Electron microscopy revealed that the perimeter of cellular fingerlike-projections was smaller in Des -/-, indicating that the cells have lost part of their connections to the extracellular matrix. The rupture pressure was significantly lower in Des -/- (1500+/-200 mmHg) compared with Des +/+ (2100+/-80 mmHg) indicating a lower mechanical strength of the vascular wall. No significant difference was found between Des +/- and Des +/+.
The desmin is essential to maintain proper viscoelastic properties, structure and mechanical strength of the vascular wall.</description><identifier>ISSN: 0008-6363</identifier><identifier>EISSN: 1755-3245</identifier><identifier>DOI: 10.1016/S0008-6363(01)00278-4</identifier><identifier>PMID: 11399260</identifier><identifier>CODEN: CVREAU</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Analysis of Variance ; Animals ; Aorta - chemistry ; Biological and medical sciences ; Biomechanical Phenomena ; Blood vessels and receptors ; Blotting, Western ; Carotid Artery, Common - diagnostic imaging ; Carotid Artery, Common - physiology ; Carotid Artery, Common - ultrastructure ; Desmin - analysis ; Desmin - deficiency ; Desmin - genetics ; Elasticity ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; In Vitro Techniques ; Mice ; Mice, Inbred C57BL ; Mice, Mutant Strains ; Microscopy, Electron ; Muscle, Smooth, Vascular - chemistry ; Muscle, Smooth, Vascular - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; Ultrasonography ; Vertebrates: cardiovascular system ; Vimentin - analysis ; Viscosity</subject><ispartof>Cardiovascular research, 2001-07, Vol.51 (1), p.178-187</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-9727f81dbc14b5c6573a319e1dd19fb9583d49f663447520472ca21e1ef9ebeb3</citedby><cites>FETCH-LOGICAL-c381t-9727f81dbc14b5c6573a319e1dd19fb9583d49f663447520472ca21e1ef9ebeb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1037007$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11399260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LACOLLEY, Patrick</creatorcontrib><creatorcontrib>CHALLANDE, Pascal</creatorcontrib><creatorcontrib>BOUMAZA, Saliha</creatorcontrib><creatorcontrib>COHUET, Géraldine</creatorcontrib><creatorcontrib>LAURENT, Stéphane</creatorcontrib><creatorcontrib>BOUTOUYRIE, Pierre</creatorcontrib><creatorcontrib>GRIMAUD, Jean-Alexis</creatorcontrib><creatorcontrib>PAULIN, Denise</creatorcontrib><creatorcontrib>LAMAZIERE, Jean-Marie Daniel</creatorcontrib><creatorcontrib>ZHENLIN LID</creatorcontrib><title>Mechanical properties and structure of carotid arteries in mice lacking desmin</title><title>Cardiovascular research</title><addtitle>Cardiovasc Res</addtitle><description>Our aim was to determine in desmin homozygous mutant mice the viscoelastic properties, the mechanical strength and the structure of the carotid artery.
To assess the viscoelastic properties of large arteries, we have performed an in vivo analysis of the diameter-, and distensibility-pressure curves of the common carotid artery (CCA) in homozygous (Des -/-), heterozygous (Des +/-) and wild-type (Des +/+) mice. To evaluate the mechanical strength, we have measured the in vitro intraluminal pressure producing the rupture of the carotid artery wall. The structure analysis of the arterial wall was based on histology and electronic microscopy.
A lower distensibility and an increase of arterial wall viscosity were observed in Des -/- compared with Des +/+. Arterial thickness of Des -/- was similar to those of Des +/+, without changes in elastin and collagen contents. Electron microscopy revealed that the perimeter of cellular fingerlike-projections was smaller in Des -/-, indicating that the cells have lost part of their connections to the extracellular matrix. The rupture pressure was significantly lower in Des -/- (1500+/-200 mmHg) compared with Des +/+ (2100+/-80 mmHg) indicating a lower mechanical strength of the vascular wall. No significant difference was found between Des +/- and Des +/+.
The desmin is essential to maintain proper viscoelastic properties, structure and mechanical strength of the vascular wall.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Aorta - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biomechanical Phenomena</subject><subject>Blood vessels and receptors</subject><subject>Blotting, Western</subject><subject>Carotid Artery, Common - diagnostic imaging</subject><subject>Carotid Artery, Common - physiology</subject><subject>Carotid Artery, Common - ultrastructure</subject><subject>Desmin - analysis</subject><subject>Desmin - deficiency</subject><subject>Desmin - genetics</subject><subject>Elasticity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Mutant Strains</subject><subject>Microscopy, Electron</subject><subject>Muscle, Smooth, Vascular - chemistry</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Ultrasonography</subject><subject>Vertebrates: cardiovascular system</subject><subject>Vimentin - analysis</subject><subject>Viscosity</subject><issn>0008-6363</issn><issn>1755-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkLlOxDAQhi0EguV4BJALhKAIeHzEcYlWXBJHAdSW40zAkGOxk4K3JwsroBqN9P3_jD5C9oGdAoP87JExVmS5yMUxgxPGuC4yuUZmoJXKBJdqncx-kS2yndLbtCql5SbZAhDG8JzNyP0d-lfXBe8auoj9AuMQMFHXVTQNcfTDGJH2NfUu9kOoqIsDxiUROtoGj7Rx_j10L7TC1IZul2zUrkm4t5o75Pny4ml-nd0-XN3Mz28zLwoYMqO5rguoSg-yVD5XWjgBBqGqwNSlUYWopKnzXEipFWdSc-84IGBtsMRS7JCjn97p548R02DbkDw2jeuwH5PVzDAtOJ9A9QP62KcUsbaLGFoXPy0wuxRpv0XapSXLwH6LtHLKHawOjGWL1V9qZW4CDleAS5O8OrrOh_SvXWg2vfAF7S57Bg</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>LACOLLEY, Patrick</creator><creator>CHALLANDE, Pascal</creator><creator>BOUMAZA, Saliha</creator><creator>COHUET, Géraldine</creator><creator>LAURENT, Stéphane</creator><creator>BOUTOUYRIE, Pierre</creator><creator>GRIMAUD, Jean-Alexis</creator><creator>PAULIN, Denise</creator><creator>LAMAZIERE, Jean-Marie Daniel</creator><creator>ZHENLIN LID</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>Mechanical properties and structure of carotid arteries in mice lacking desmin</title><author>LACOLLEY, Patrick ; CHALLANDE, Pascal ; BOUMAZA, Saliha ; COHUET, Géraldine ; LAURENT, Stéphane ; BOUTOUYRIE, Pierre ; GRIMAUD, Jean-Alexis ; PAULIN, Denise ; LAMAZIERE, Jean-Marie Daniel ; ZHENLIN LID</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-9727f81dbc14b5c6573a319e1dd19fb9583d49f663447520472ca21e1ef9ebeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Aorta - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biomechanical Phenomena</topic><topic>Blood vessels and receptors</topic><topic>Blotting, Western</topic><topic>Carotid Artery, Common - diagnostic imaging</topic><topic>Carotid Artery, Common - physiology</topic><topic>Carotid Artery, Common - ultrastructure</topic><topic>Desmin - analysis</topic><topic>Desmin - deficiency</topic><topic>Desmin - genetics</topic><topic>Elasticity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Mutant Strains</topic><topic>Microscopy, Electron</topic><topic>Muscle, Smooth, Vascular - chemistry</topic><topic>Muscle, Smooth, Vascular - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Ultrasonography</topic><topic>Vertebrates: cardiovascular system</topic><topic>Vimentin - analysis</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LACOLLEY, Patrick</creatorcontrib><creatorcontrib>CHALLANDE, Pascal</creatorcontrib><creatorcontrib>BOUMAZA, Saliha</creatorcontrib><creatorcontrib>COHUET, Géraldine</creatorcontrib><creatorcontrib>LAURENT, Stéphane</creatorcontrib><creatorcontrib>BOUTOUYRIE, Pierre</creatorcontrib><creatorcontrib>GRIMAUD, Jean-Alexis</creatorcontrib><creatorcontrib>PAULIN, Denise</creatorcontrib><creatorcontrib>LAMAZIERE, Jean-Marie Daniel</creatorcontrib><creatorcontrib>ZHENLIN LID</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cardiovascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LACOLLEY, Patrick</au><au>CHALLANDE, Pascal</au><au>BOUMAZA, Saliha</au><au>COHUET, Géraldine</au><au>LAURENT, Stéphane</au><au>BOUTOUYRIE, Pierre</au><au>GRIMAUD, Jean-Alexis</au><au>PAULIN, Denise</au><au>LAMAZIERE, Jean-Marie Daniel</au><au>ZHENLIN LID</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanical properties and structure of carotid arteries in mice lacking desmin</atitle><jtitle>Cardiovascular research</jtitle><addtitle>Cardiovasc Res</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>51</volume><issue>1</issue><spage>178</spage><epage>187</epage><pages>178-187</pages><issn>0008-6363</issn><eissn>1755-3245</eissn><coden>CVREAU</coden><abstract>Our aim was to determine in desmin homozygous mutant mice the viscoelastic properties, the mechanical strength and the structure of the carotid artery.
To assess the viscoelastic properties of large arteries, we have performed an in vivo analysis of the diameter-, and distensibility-pressure curves of the common carotid artery (CCA) in homozygous (Des -/-), heterozygous (Des +/-) and wild-type (Des +/+) mice. To evaluate the mechanical strength, we have measured the in vitro intraluminal pressure producing the rupture of the carotid artery wall. The structure analysis of the arterial wall was based on histology and electronic microscopy.
A lower distensibility and an increase of arterial wall viscosity were observed in Des -/- compared with Des +/+. Arterial thickness of Des -/- was similar to those of Des +/+, without changes in elastin and collagen contents. Electron microscopy revealed that the perimeter of cellular fingerlike-projections was smaller in Des -/-, indicating that the cells have lost part of their connections to the extracellular matrix. The rupture pressure was significantly lower in Des -/- (1500+/-200 mmHg) compared with Des +/+ (2100+/-80 mmHg) indicating a lower mechanical strength of the vascular wall. No significant difference was found between Des +/- and Des +/+.
The desmin is essential to maintain proper viscoelastic properties, structure and mechanical strength of the vascular wall.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>11399260</pmid><doi>10.1016/S0008-6363(01)00278-4</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cardiovascular research, 2001-07, Vol.51 (1), p.178-187 |
| issn | 0008-6363 1755-3245 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Analysis of Variance Animals Aorta - chemistry Biological and medical sciences Biomechanical Phenomena Blood vessels and receptors Blotting, Western Carotid Artery, Common - diagnostic imaging Carotid Artery, Common - physiology Carotid Artery, Common - ultrastructure Desmin - analysis Desmin - deficiency Desmin - genetics Elasticity Fundamental and applied biological sciences. Psychology Immunohistochemistry In Vitro Techniques Mice Mice, Inbred C57BL Mice, Mutant Strains Microscopy, Electron Muscle, Smooth, Vascular - chemistry Muscle, Smooth, Vascular - physiology Reverse Transcriptase Polymerase Chain Reaction Ultrasonography Vertebrates: cardiovascular system Vimentin - analysis Viscosity |
| title | Mechanical properties and structure of carotid arteries in mice lacking desmin |
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