Different Regulatory Mechanisms Modulate the Expression of a Dinoflagellate Iron-Superoxide Dismutase
Regulation of antioxidant enzymes is critical to control the levels of reactive oxygen species in cell compartments highly susceptible to oxidative stress. In this work, we studied the regulation of a chloroplastic iron superoxide dismutase (Fe-SOD) fromLingulodinium polyedrum (formerly Gonyaulax po...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 2001-06, Vol.276 (23), p.19989-19993 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 19993 |
|---|---|
| container_issue | 23 |
| container_start_page | 19989 |
| container_title | The Journal of biological chemistry |
| container_volume | 276 |
| creator | Okamoto, O. Keith Robertson, Deborah L. Fagan, Thomas F. Hastings, J. Woodland Colepicolo, Pio |
| description | Regulation of antioxidant enzymes is critical to control the levels of reactive oxygen species in cell compartments highly susceptible to oxidative stress. In this work, we studied the regulation of a chloroplastic iron superoxide dismutase (Fe-SOD) fromLingulodinium polyedrum (formerly Gonyaulax polyedra) under different physiological conditions. A cDNA-encoding Fe-SOD was isolated from this dinoflagellate, showing high sequence similarity to cyanobacterial, algal, and plant Fe-SODs. Under standard growth conditions, on a 12:12-h light-dark cycle,Lingulodinium polyedrum Fe-SOD exhibited a daily rhythm of activity and cellular abundance with the maximum occurring during the middle of the light phase. Northern analyses showed that this rhythmicity is not related to changes in Fe-SOD mRNA levels, indicative of translational regulation. By contrast, conditions of metal-induced oxidative stress resulted in higher levels of Fe-SOD transcripts, suggesting that transcriptional control is responsible for increased protein and activity levels. Daily (circadian) and metal-induced up-regulation of Fe-SOD expression in L. polyedrum are thus mediated by different regulatory pathways, allowing biochemically distinct changes appropriate to oxidative challenges. |
| doi_str_mv | 10.1074/jbc.M101169200 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70905816</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021925819404328</els_id><sourcerecordid>18074232</sourcerecordid><originalsourceid>FETCH-LOGICAL-c440t-a9681cb53d6334d714986656ecc090dbbdbe371bf2a672b431bc74422773fcd43</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS0EokvhyhHlgLhl8diOHR9RW6BSV0h8SNys2J5sXG3ixU6g_e_xdlfqCTGXkWZ-8zR6j5DXQNdAlXh_a916AxRAakbpE7IC2vKaN_DzKVlRyqDWrGnPyIucb2kpoeE5OQNgUrBWrwhehr7HhNNcfcXtsuvmmO6rDbqhm0Iec7WJ_jDFah6wurrbJ8w5xKmKfdVVl2GK_a7b4u4BuU5xqr8te0zxLngs6zwuc5fxJXnWd7uMr079nPz4ePX94nN98-XT9cWHm9oJQee607IFZxvuJefCKxC6lbKR6BzV1FvrLXIFtmedVMwKDtYpIRhTivfOC35O3h119yn-WjDPZgzZHb6bMC7ZqCLTtCD_C0JbzGWcFXB9BF2KOSfszT6FsUv3Bqg5JGBKAuYxgXLw5qS82BH9I36yvABvj8AQtsOfkNDYEN2Ao2FKGsYNaP2AtUcMi1-_AyaTXcDJoS8nbjY-hn-98Bc-eKDd</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18074232</pqid></control><display><type>article</type><title>Different Regulatory Mechanisms Modulate the Expression of a Dinoflagellate Iron-Superoxide Dismutase</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Okamoto, O. Keith ; Robertson, Deborah L. ; Fagan, Thomas F. ; Hastings, J. Woodland ; Colepicolo, Pio</creator><creatorcontrib>Okamoto, O. Keith ; Robertson, Deborah L. ; Fagan, Thomas F. ; Hastings, J. Woodland ; Colepicolo, Pio</creatorcontrib><description>Regulation of antioxidant enzymes is critical to control the levels of reactive oxygen species in cell compartments highly susceptible to oxidative stress. In this work, we studied the regulation of a chloroplastic iron superoxide dismutase (Fe-SOD) fromLingulodinium polyedrum (formerly Gonyaulax polyedra) under different physiological conditions. A cDNA-encoding Fe-SOD was isolated from this dinoflagellate, showing high sequence similarity to cyanobacterial, algal, and plant Fe-SODs. Under standard growth conditions, on a 12:12-h light-dark cycle,Lingulodinium polyedrum Fe-SOD exhibited a daily rhythm of activity and cellular abundance with the maximum occurring during the middle of the light phase. Northern analyses showed that this rhythmicity is not related to changes in Fe-SOD mRNA levels, indicative of translational regulation. By contrast, conditions of metal-induced oxidative stress resulted in higher levels of Fe-SOD transcripts, suggesting that transcriptional control is responsible for increased protein and activity levels. Daily (circadian) and metal-induced up-regulation of Fe-SOD expression in L. polyedrum are thus mediated by different regulatory pathways, allowing biochemically distinct changes appropriate to oxidative challenges.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M101169200</identifier><identifier>PMID: 11264289</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Northern ; Dinoflagellida ; DNA Primers ; DNA, Complementary ; Lingulodinium polyedrum ; Metals ; Molecular Sequence Data ; Oxidative Stress ; Protein Biosynthesis ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid ; Superoxide Dismutase - chemistry ; Superoxide Dismutase - genetics ; Superoxide Dismutase - metabolism</subject><ispartof>The Journal of biological chemistry, 2001-06, Vol.276 (23), p.19989-19993</ispartof><rights>2001 © 2001 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-a9681cb53d6334d714986656ecc090dbbdbe371bf2a672b431bc74422773fcd43</citedby><cites>FETCH-LOGICAL-c440t-a9681cb53d6334d714986656ecc090dbbdbe371bf2a672b431bc74422773fcd43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11264289$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okamoto, O. Keith</creatorcontrib><creatorcontrib>Robertson, Deborah L.</creatorcontrib><creatorcontrib>Fagan, Thomas F.</creatorcontrib><creatorcontrib>Hastings, J. Woodland</creatorcontrib><creatorcontrib>Colepicolo, Pio</creatorcontrib><title>Different Regulatory Mechanisms Modulate the Expression of a Dinoflagellate Iron-Superoxide Dismutase</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Regulation of antioxidant enzymes is critical to control the levels of reactive oxygen species in cell compartments highly susceptible to oxidative stress. In this work, we studied the regulation of a chloroplastic iron superoxide dismutase (Fe-SOD) fromLingulodinium polyedrum (formerly Gonyaulax polyedra) under different physiological conditions. A cDNA-encoding Fe-SOD was isolated from this dinoflagellate, showing high sequence similarity to cyanobacterial, algal, and plant Fe-SODs. Under standard growth conditions, on a 12:12-h light-dark cycle,Lingulodinium polyedrum Fe-SOD exhibited a daily rhythm of activity and cellular abundance with the maximum occurring during the middle of the light phase. Northern analyses showed that this rhythmicity is not related to changes in Fe-SOD mRNA levels, indicative of translational regulation. By contrast, conditions of metal-induced oxidative stress resulted in higher levels of Fe-SOD transcripts, suggesting that transcriptional control is responsible for increased protein and activity levels. Daily (circadian) and metal-induced up-regulation of Fe-SOD expression in L. polyedrum are thus mediated by different regulatory pathways, allowing biochemically distinct changes appropriate to oxidative challenges.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Dinoflagellida</subject><subject>DNA Primers</subject><subject>DNA, Complementary</subject><subject>Lingulodinium polyedrum</subject><subject>Metals</subject><subject>Molecular Sequence Data</subject><subject>Oxidative Stress</subject><subject>Protein Biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Superoxide Dismutase - chemistry</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EokvhyhHlgLhl8diOHR9RW6BSV0h8SNys2J5sXG3ixU6g_e_xdlfqCTGXkWZ-8zR6j5DXQNdAlXh_a916AxRAakbpE7IC2vKaN_DzKVlRyqDWrGnPyIucb2kpoeE5OQNgUrBWrwhehr7HhNNcfcXtsuvmmO6rDbqhm0Iec7WJ_jDFah6wurrbJ8w5xKmKfdVVl2GK_a7b4u4BuU5xqr8te0zxLngs6zwuc5fxJXnWd7uMr079nPz4ePX94nN98-XT9cWHm9oJQee607IFZxvuJefCKxC6lbKR6BzV1FvrLXIFtmedVMwKDtYpIRhTivfOC35O3h119yn-WjDPZgzZHb6bMC7ZqCLTtCD_C0JbzGWcFXB9BF2KOSfszT6FsUv3Bqg5JGBKAuYxgXLw5qS82BH9I36yvABvj8AQtsOfkNDYEN2Ao2FKGsYNaP2AtUcMi1-_AyaTXcDJoS8nbjY-hn-98Bc-eKDd</recordid><startdate>20010608</startdate><enddate>20010608</enddate><creator>Okamoto, O. Keith</creator><creator>Robertson, Deborah L.</creator><creator>Fagan, Thomas F.</creator><creator>Hastings, J. Woodland</creator><creator>Colepicolo, Pio</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20010608</creationdate><title>Different Regulatory Mechanisms Modulate the Expression of a Dinoflagellate Iron-Superoxide Dismutase</title><author>Okamoto, O. Keith ; Robertson, Deborah L. ; Fagan, Thomas F. ; Hastings, J. Woodland ; Colepicolo, Pio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-a9681cb53d6334d714986656ecc090dbbdbe371bf2a672b431bc74422773fcd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Dinoflagellida</topic><topic>DNA Primers</topic><topic>DNA, Complementary</topic><topic>Lingulodinium polyedrum</topic><topic>Metals</topic><topic>Molecular Sequence Data</topic><topic>Oxidative Stress</topic><topic>Protein Biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Superoxide Dismutase - chemistry</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okamoto, O. Keith</creatorcontrib><creatorcontrib>Robertson, Deborah L.</creatorcontrib><creatorcontrib>Fagan, Thomas F.</creatorcontrib><creatorcontrib>Hastings, J. Woodland</creatorcontrib><creatorcontrib>Colepicolo, Pio</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okamoto, O. Keith</au><au>Robertson, Deborah L.</au><au>Fagan, Thomas F.</au><au>Hastings, J. Woodland</au><au>Colepicolo, Pio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different Regulatory Mechanisms Modulate the Expression of a Dinoflagellate Iron-Superoxide Dismutase</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2001-06-08</date><risdate>2001</risdate><volume>276</volume><issue>23</issue><spage>19989</spage><epage>19993</epage><pages>19989-19993</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Regulation of antioxidant enzymes is critical to control the levels of reactive oxygen species in cell compartments highly susceptible to oxidative stress. In this work, we studied the regulation of a chloroplastic iron superoxide dismutase (Fe-SOD) fromLingulodinium polyedrum (formerly Gonyaulax polyedra) under different physiological conditions. A cDNA-encoding Fe-SOD was isolated from this dinoflagellate, showing high sequence similarity to cyanobacterial, algal, and plant Fe-SODs. Under standard growth conditions, on a 12:12-h light-dark cycle,Lingulodinium polyedrum Fe-SOD exhibited a daily rhythm of activity and cellular abundance with the maximum occurring during the middle of the light phase. Northern analyses showed that this rhythmicity is not related to changes in Fe-SOD mRNA levels, indicative of translational regulation. By contrast, conditions of metal-induced oxidative stress resulted in higher levels of Fe-SOD transcripts, suggesting that transcriptional control is responsible for increased protein and activity levels. Daily (circadian) and metal-induced up-regulation of Fe-SOD expression in L. polyedrum are thus mediated by different regulatory pathways, allowing biochemically distinct changes appropriate to oxidative challenges.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11264289</pmid><doi>10.1074/jbc.M101169200</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 2001-06, Vol.276 (23), p.19989-19993 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70905816 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Base Sequence Blotting, Northern Dinoflagellida DNA Primers DNA, Complementary Lingulodinium polyedrum Metals Molecular Sequence Data Oxidative Stress Protein Biosynthesis RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid Superoxide Dismutase - chemistry Superoxide Dismutase - genetics Superoxide Dismutase - metabolism |
| title | Different Regulatory Mechanisms Modulate the Expression of a Dinoflagellate Iron-Superoxide Dismutase |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T12%3A41%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Different%20Regulatory%20Mechanisms%20Modulate%20the%20Expression%20of%20a%20Dinoflagellate%20Iron-Superoxide%20Dismutase&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Okamoto,%20O.%20Keith&rft.date=2001-06-08&rft.volume=276&rft.issue=23&rft.spage=19989&rft.epage=19993&rft.pages=19989-19993&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1074/jbc.M101169200&rft_dat=%3Cproquest_cross%3E18074232%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18074232&rft_id=info:pmid/11264289&rft_els_id=S0021925819404328&rfr_iscdi=true |