Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6
The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1–34), (67–86), or (107–139) to growth medium did not affect proliferation of cells grown in either high (10%...
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| Veröffentlicht in: | Regulatory peptides 2001-06, Vol.99 (2), p.169-174 |
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| creator | Ye, Yumei Falzon, Miriam Seitz, Patricia K Cooper, Cary W |
| description | The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1–34), (67–86), or (107–139) to growth medium did not affect proliferation of cells grown in either high (10% Nu-Serum) or low serum (1% Nu-Serum). However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and
3H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate
3H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects. |
| doi_str_mv | 10.1016/S0167-0115(01)00248-8 |
| format | Article |
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3H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate
3H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects.</description><identifier>ISSN: 0167-0115</identifier><identifier>EISSN: 1873-1686</identifier><identifier>DOI: 10.1016/S0167-0115(01)00248-8</identifier><identifier>PMID: 11384779</identifier><identifier>CODEN: REPPDY</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cell Division - genetics ; Cell Line ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors - chemical synthesis ; GI epithelium ; Humans ; Intestine. Mesentery ; Nuclear localization signal ; Nuclear Localization Signals - genetics ; Parathyroid Hormone-Related Protein ; Peptide Fragments - pharmacology ; Protein Biosynthesis ; Proteins - genetics ; Proteins - pharmacology ; Proteins - physiology ; Rats ; Sequence Deletion - genetics ; Transfection ; Vertebrates: digestive system</subject><ispartof>Regulatory peptides, 2001-06, Vol.99 (2), p.169-174</ispartof><rights>2001</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-65773df50f905f1a56d292b71ac5c5ad3ac53264b915d35dde21dafdb2f29f103</citedby><cites>FETCH-LOGICAL-c390t-65773df50f905f1a56d292b71ac5c5ad3ac53264b915d35dde21dafdb2f29f103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0167-0115(01)00248-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1084579$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11384779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ye, Yumei</creatorcontrib><creatorcontrib>Falzon, Miriam</creatorcontrib><creatorcontrib>Seitz, Patricia K</creatorcontrib><creatorcontrib>Cooper, Cary W</creatorcontrib><title>Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6</title><title>Regulatory peptides</title><addtitle>Regul Pept</addtitle><description>The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1–34), (67–86), or (107–139) to growth medium did not affect proliferation of cells grown in either high (10% Nu-Serum) or low serum (1% Nu-Serum). However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and
3H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate
3H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Division - genetics</subject><subject>Cell Line</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors - chemical synthesis</subject><subject>GI epithelium</subject><subject>Humans</subject><subject>Intestine. Mesentery</subject><subject>Nuclear localization signal</subject><subject>Nuclear Localization Signals - genetics</subject><subject>Parathyroid Hormone-Related Protein</subject><subject>Peptide Fragments - pharmacology</subject><subject>Protein Biosynthesis</subject><subject>Proteins - genetics</subject><subject>Proteins - pharmacology</subject><subject>Proteins - physiology</subject><subject>Rats</subject><subject>Sequence Deletion - genetics</subject><subject>Transfection</subject><subject>Vertebrates: digestive system</subject><issn>0167-0115</issn><issn>1873-1686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EgvL4BJAXCMEi4EnqPFYIVbwkJBbA2nLtMTVK4mCnBf4ep62AHZsZyz4zvjqEHAI7Bwb5xVMsRcIA-CmDM8bScZmUG2QEZZElkJf5Jhn9IDtkN4Q3xoAXRbZNdgCyclwU1Yh0jwv0-Nl5DMG6ljpDO-llP_vyzmo6c75xLSYea9mjpp13Pdp26E08Baqwrumrdx_9jMb7foY0TsdjfOxtK-sVUdsW6f31JMn3yZaRdcCDdd8jLzfXz5O75OHx9n5y9ZCorGJ9kg9JteHMVIwbkDzXaZVOC5CKKy51FnuW5uNpBVxnXGtMQUujp6lJKwMs2yMnq70x6vs8hhGNDUMW2aKbB1GwikF0EEG-ApV3IXg0ovO2kf5LABODarFULQaPsYilalHGuaP1B_Npg_p3au02AsdrQAYla-Nlq2z4s70c8yV2ucIw2lhY9CIoi61CbT2qXmhn_0nyDa3HnDw</recordid><startdate>20010615</startdate><enddate>20010615</enddate><creator>Ye, Yumei</creator><creator>Falzon, Miriam</creator><creator>Seitz, Patricia K</creator><creator>Cooper, Cary W</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010615</creationdate><title>Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6</title><author>Ye, Yumei ; Falzon, Miriam ; Seitz, Patricia K ; Cooper, Cary W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-65773df50f905f1a56d292b71ac5c5ad3ac53264b915d35dde21dafdb2f29f103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Division - genetics</topic><topic>Cell Line</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Vectors - chemical synthesis</topic><topic>GI epithelium</topic><topic>Humans</topic><topic>Intestine. Mesentery</topic><topic>Nuclear localization signal</topic><topic>Nuclear Localization Signals - genetics</topic><topic>Parathyroid Hormone-Related Protein</topic><topic>Peptide Fragments - pharmacology</topic><topic>Protein Biosynthesis</topic><topic>Proteins - genetics</topic><topic>Proteins - pharmacology</topic><topic>Proteins - physiology</topic><topic>Rats</topic><topic>Sequence Deletion - genetics</topic><topic>Transfection</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ye, Yumei</creatorcontrib><creatorcontrib>Falzon, Miriam</creatorcontrib><creatorcontrib>Seitz, Patricia K</creatorcontrib><creatorcontrib>Cooper, Cary W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Regulatory peptides</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ye, Yumei</au><au>Falzon, Miriam</au><au>Seitz, Patricia K</au><au>Cooper, Cary W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6</atitle><jtitle>Regulatory peptides</jtitle><addtitle>Regul Pept</addtitle><date>2001-06-15</date><risdate>2001</risdate><volume>99</volume><issue>2</issue><spage>169</spage><epage>174</epage><pages>169-174</pages><issn>0167-0115</issn><eissn>1873-1686</eissn><coden>REPPDY</coden><abstract>The rat intestinal cell line, IEC-6, was used as a model to study effects of parathyroid hormone-related protein (PTHrP) on crypt cell growth. Studies showed that addition of PTHrP analogs (1–34), (67–86), or (107–139) to growth medium did not affect proliferation of cells grown in either high (10% Nu-Serum) or low serum (1% Nu-Serum). However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and
3H-thymidine incorporation in high, but not low, serum. Additional studies examined the role of the nuclear localization sequence (NLS) of PTHrP in mediating the growth effect. In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate
3H-thymidine incorporation and cell growth was lost. The results suggest that endogenously produced PTHrP can promote proliferation of IEC-6 cells and that the integrity of the NLS of PTHrP is required for its growth effects.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11384779</pmid><doi>10.1016/S0167-0115(01)00248-8</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cell Division - genetics Cell Line Fundamental and applied biological sciences. Psychology Genetic Vectors - chemical synthesis GI epithelium Humans Intestine. Mesentery Nuclear localization signal Nuclear Localization Signals - genetics Parathyroid Hormone-Related Protein Peptide Fragments - pharmacology Protein Biosynthesis Proteins - genetics Proteins - pharmacology Proteins - physiology Rats Sequence Deletion - genetics Transfection Vertebrates: digestive system |
| title | Overexpression of parathyroid hormone-related protein promotes cell growth in the rat intestinal cell line IEC-6 |
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