Induction of CYP1A1 by Serum Independent of AhR Pathway
CYP1A1 is implicated in the bioactivation of procarcinogens such as polycyclic aromatic hydrocarbons. To date, no physiological compounds have been described as inducers of this gene. In this study, we have examined the role of serum in the regulation of CYP1A1 gene expression. After treatment of Ca...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-01, Vol.267 (2), p.572-576 |
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| creator | Guigal, Nolwen Seree, Eric Bourgarel-Rey, Véronique Barra, Yves |
| description | CYP1A1 is implicated in the bioactivation of procarcinogens such as polycyclic aromatic hydrocarbons. To date, no physiological compounds have been described as inducers of this gene. In this study, we have examined the role of serum in the regulation of CYP1A1 gene expression. After treatment of CaCo-2 cells with fetal bovine serum, CYP1A1 mRNA level increased to the same extent as that observed after 3-methylcholanthrene induction. The same effect was obtained after treatment with adult bovine or human serum. Evaluation of hnRNA level performed on CaCo-2 cells indicates that CYP1A1 induction by serum acts at least in part through transcriptional activation. Promoter region containing the XRE (1.56 kb) was tested in the CAT assay. No stimulation of this reporter gene was detected after serum treatment. These results demonstrate for the first time that physiological compound(s) contained in serum induces CYP1A1 gene expression by transcriptional activation independent of the AhR pathway. |
| doi_str_mv | 10.1006/bbrc.1999.1959 |
| format | Article |
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To date, no physiological compounds have been described as inducers of this gene. In this study, we have examined the role of serum in the regulation of CYP1A1 gene expression. After treatment of CaCo-2 cells with fetal bovine serum, CYP1A1 mRNA level increased to the same extent as that observed after 3-methylcholanthrene induction. The same effect was obtained after treatment with adult bovine or human serum. Evaluation of hnRNA level performed on CaCo-2 cells indicates that CYP1A1 induction by serum acts at least in part through transcriptional activation. Promoter region containing the XRE (1.56 kb) was tested in the CAT assay. No stimulation of this reporter gene was detected after serum treatment. 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To date, no physiological compounds have been described as inducers of this gene. In this study, we have examined the role of serum in the regulation of CYP1A1 gene expression. After treatment of CaCo-2 cells with fetal bovine serum, CYP1A1 mRNA level increased to the same extent as that observed after 3-methylcholanthrene induction. The same effect was obtained after treatment with adult bovine or human serum. Evaluation of hnRNA level performed on CaCo-2 cells indicates that CYP1A1 induction by serum acts at least in part through transcriptional activation. Promoter region containing the XRE (1.56 kb) was tested in the CAT assay. No stimulation of this reporter gene was detected after serum treatment. These results demonstrate for the first time that physiological compound(s) contained in serum induces CYP1A1 gene expression by transcriptional activation independent of the AhR pathway.</description><subject>3-methylcholanthrene</subject><subject>Adult</subject><subject>AhR-independent pathway</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Caco-2 Cells</subject><subject>Cattle</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Culture Media</subject><subject>CYP1A1 induction</subject><subject>CYP1A1 protein</subject><subject>Cytochrome P-450 CYP1A1 - biosynthesis</subject><subject>Cytochrome P-450 CYP1A1 - genetics</subject><subject>cytochrome P450</subject><subject>DNA Primers - genetics</subject><subject>Enzyme Induction</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Genes, Reporter</subject><subject>hnRNA</subject><subject>Humans</subject><subject>Methylcholanthrene - pharmacology</subject><subject>Receptors, Aryl Hydrocarbon - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>serum</subject><subject>Transcriptional Activation - drug effects</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAURi0EoqWwMqJMbCm-cWzHY1XxqFSJiocEk5X4oQY1SbETUP49jtKBBbF8d7jnftI9CF0CngPG7KYonJqDECIEFUdoCljgOAGcHqMpDkScCHiboDPvPzAGSJk4RRPAjABgMkV8VetOtWVTR42Nlu8bWEBU9NGzcV0VhaXZmxB1O6wX26dok7fb77w_Ryc233lzcZgz9Hp3-7J8iNeP96vlYh2rlPA2Zgy0JSlJgSqcgKacMEYTzVMrKGS2EIQCsZQnlgkNGVhBRKqgICYnlGkyQ9dj7941n53xraxKr8xul9em6bzk4V0gDP4FgWdCAOcBnI-gco33zli5d2WVu14CloNTOTiVg1M5OA0HV4fmrqiM_oWPEgOQjYAJIr5K46RXpamV0aUzqpW6Kf_q_gFxvIHE</recordid><startdate>20000119</startdate><enddate>20000119</enddate><creator>Guigal, Nolwen</creator><creator>Seree, Eric</creator><creator>Bourgarel-Rey, Véronique</creator><creator>Barra, Yves</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000119</creationdate><title>Induction of CYP1A1 by Serum Independent of AhR Pathway</title><author>Guigal, Nolwen ; Seree, Eric ; Bourgarel-Rey, Véronique ; Barra, Yves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-661df343415c021d5736652d74f9518fb93513f572f69d181f9394c1b3ea356d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>3-methylcholanthrene</topic><topic>Adult</topic><topic>AhR-independent pathway</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Caco-2 Cells</topic><topic>Cattle</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Culture Media</topic><topic>CYP1A1 induction</topic><topic>CYP1A1 protein</topic><topic>Cytochrome P-450 CYP1A1 - biosynthesis</topic><topic>Cytochrome P-450 CYP1A1 - genetics</topic><topic>cytochrome P450</topic><topic>DNA Primers - genetics</topic><topic>Enzyme Induction</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Genes, Reporter</topic><topic>hnRNA</topic><topic>Humans</topic><topic>Methylcholanthrene - pharmacology</topic><topic>Receptors, Aryl Hydrocarbon - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>serum</topic><topic>Transcriptional Activation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guigal, Nolwen</creatorcontrib><creatorcontrib>Seree, Eric</creatorcontrib><creatorcontrib>Bourgarel-Rey, Véronique</creatorcontrib><creatorcontrib>Barra, Yves</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guigal, Nolwen</au><au>Seree, Eric</au><au>Bourgarel-Rey, Véronique</au><au>Barra, Yves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of CYP1A1 by Serum Independent of AhR Pathway</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2000-01-19</date><risdate>2000</risdate><volume>267</volume><issue>2</issue><spage>572</spage><epage>576</epage><pages>572-576</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>CYP1A1 is implicated in the bioactivation of procarcinogens such as polycyclic aromatic hydrocarbons. To date, no physiological compounds have been described as inducers of this gene. In this study, we have examined the role of serum in the regulation of CYP1A1 gene expression. After treatment of CaCo-2 cells with fetal bovine serum, CYP1A1 mRNA level increased to the same extent as that observed after 3-methylcholanthrene induction. The same effect was obtained after treatment with adult bovine or human serum. Evaluation of hnRNA level performed on CaCo-2 cells indicates that CYP1A1 induction by serum acts at least in part through transcriptional activation. Promoter region containing the XRE (1.56 kb) was tested in the CAT assay. No stimulation of this reporter gene was detected after serum treatment. These results demonstrate for the first time that physiological compound(s) contained in serum induces CYP1A1 gene expression by transcriptional activation independent of the AhR pathway.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10631103</pmid><doi>10.1006/bbrc.1999.1959</doi><tpages>5</tpages></addata></record> |
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| subjects | 3-methylcholanthrene Adult AhR-independent pathway Animals Base Sequence Caco-2 Cells Cattle Chloramphenicol O-Acetyltransferase - genetics Culture Media CYP1A1 induction CYP1A1 protein Cytochrome P-450 CYP1A1 - biosynthesis Cytochrome P-450 CYP1A1 - genetics cytochrome P450 DNA Primers - genetics Enzyme Induction Gene Expression Regulation, Enzymologic - drug effects Genes, Reporter hnRNA Humans Methylcholanthrene - pharmacology Receptors, Aryl Hydrocarbon - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism serum Transcriptional Activation - drug effects |
| title | Induction of CYP1A1 by Serum Independent of AhR Pathway |
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