Functional conservation of atonal and Math1 in the CNS and PNS
To determine the extent to which atonal and its mouse homolog Math1 exhibit functional conservation, we inserted (beta)-galactosidase (lacZ) into the Math1 locus and analyzed its expression, evaluated consequences of loss of Math1 function, and expressed Math1 in atonal mutant flies. lacZ under the...
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| Veröffentlicht in: | Development (Cambridge) 2000-03, Vol.127 (5), p.1039-1048 |
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| container_title | Development (Cambridge) |
| container_volume | 127 |
| creator | Ben-Arie, N. Hassan, B. A. Bermingham, N. A. Malicki, D. M. Armstrong, D. Matzuk, M. Bellen, H. J. Zoghbi, H. Y. |
| description | To determine the extent to which atonal and its mouse homolog Math1 exhibit functional conservation, we inserted (beta)-galactosidase (lacZ) into the Math1 locus and analyzed its expression, evaluated consequences of loss of Math1 function, and expressed Math1 in atonal mutant flies. lacZ under the control of Math1 regulatory elements duplicated the previously known expression pattern of Math1 in the CNS (i.e., the neural tube, dorsal spinal cord, brainstem, and cerebellar external granule neurons) but also revealed new sites of expression: PNS mechanoreceptors (inner ear hair cells and Merkel cells) and articular chondrocytes. Expressing Math1 induced ectopic chordotonal organs (CHOs) in wild-type flies and partially rescued CHO loss in atonal mutant embryos. These data demonstrate that both the mouse and fly homologs encode lineage identity information and, more interestingly, that some of the cells dependent on this information serve similar mechanoreceptor functions. |
| doi_str_mv | 10.1242/dev.127.5.1039 |
| format | Article |
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A. ; Bermingham, N. A. ; Malicki, D. M. ; Armstrong, D. ; Matzuk, M. ; Bellen, H. J. ; Zoghbi, H. Y.</creator><creatorcontrib>Ben-Arie, N. ; Hassan, B. A. ; Bermingham, N. A. ; Malicki, D. M. ; Armstrong, D. ; Matzuk, M. ; Bellen, H. J. ; Zoghbi, H. Y.</creatorcontrib><description>To determine the extent to which atonal and its mouse homolog Math1 exhibit functional conservation, we inserted (beta)-galactosidase (lacZ) into the Math1 locus and analyzed its expression, evaluated consequences of loss of Math1 function, and expressed Math1 in atonal mutant flies. lacZ under the control of Math1 regulatory elements duplicated the previously known expression pattern of Math1 in the CNS (i.e., the neural tube, dorsal spinal cord, brainstem, and cerebellar external granule neurons) but also revealed new sites of expression: PNS mechanoreceptors (inner ear hair cells and Merkel cells) and articular chondrocytes. Expressing Math1 induced ectopic chordotonal organs (CHOs) in wild-type flies and partially rescued CHO loss in atonal mutant embryos. 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Y.</creatorcontrib><title>Functional conservation of atonal and Math1 in the CNS and PNS</title><title>Development (Cambridge)</title><addtitle>Development</addtitle><description>To determine the extent to which atonal and its mouse homolog Math1 exhibit functional conservation, we inserted (beta)-galactosidase (lacZ) into the Math1 locus and analyzed its expression, evaluated consequences of loss of Math1 function, and expressed Math1 in atonal mutant flies. lacZ under the control of Math1 regulatory elements duplicated the previously known expression pattern of Math1 in the CNS (i.e., the neural tube, dorsal spinal cord, brainstem, and cerebellar external granule neurons) but also revealed new sites of expression: PNS mechanoreceptors (inner ear hair cells and Merkel cells) and articular chondrocytes. Expressing Math1 induced ectopic chordotonal organs (CHOs) in wild-type flies and partially rescued CHO loss in atonal mutant embryos. These data demonstrate that both the mouse and fly homologs encode lineage identity information and, more interestingly, that some of the cells dependent on this information serve similar mechanoreceptor functions.</description><subject>Animals</subject><subject>atonal gene</subject><subject>Basic Helix-Loop-Helix Transcription Factors</subject><subject>beta-Galactosidase - analysis</subject><subject>beta-Galactosidase - genetics</subject><subject>Central Nervous System - embryology</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Drosophila</subject><subject>Drosophila - embryology</subject><subject>Drosophila Proteins</subject><subject>Embryo, Nonmammalian - embryology</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Helix-Loop-Helix Motifs</subject><subject>lacZ gene</subject><subject>Life Sciences (General)</subject><subject>Male</subject><subject>Math1 gene</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Organ Specificity</subject><subject>Peripheral Nervous System - embryology</subject><subject>Transcription Factors - genetics</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>CYI</sourceid><sourceid>EIF</sourceid><recordid>eNqFkDFPwzAQhS0EoqWwMiGUiS3hLnZie0FCFQUkKEiF2XISpw1K4xInRfx7kqZDN6a7e_fpPekRcokQYMjC28xsu4UHUYBA5REZI-PclxjKYzIGGYGPUuKInDn3BQA05vyUjBDiOIwZHZO7WVulTWErXXqprZypt7o_PZt7utnJusq8V92s0Csqr1kZbzpf7MT3-eKcnOS6dOZiPyfkc_bwMX3yX94en6f3L37KGG98wSPJAXPWZfI0TigajJLQSJPxLBExFTSBVGcyYYaLyDDIEyoECwXVyCWjE3Iz-G5q-90a16h14VJTlroytnWKg5CyK-BfEHkEEUIPBgOY1ta52uRqUxdrXf8qBNVXq7pqu4WrSOHgfL13bpO1yQ7wocsOuBqASjutqqZ2KgRggAxjAd3bH96rYrn6KWqjksKWdlm4xvVZprSbw7w_nRWKGg</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Ben-Arie, N.</creator><creator>Hassan, B. A.</creator><creator>Bermingham, N. A.</creator><creator>Malicki, D. M.</creator><creator>Armstrong, D.</creator><creator>Matzuk, M.</creator><creator>Bellen, H. J.</creator><creator>Zoghbi, H. Y.</creator><general>The Company of Biologists Limited</general><scope>CYE</scope><scope>CYI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Functional conservation of atonal and Math1 in the CNS and PNS</title><author>Ben-Arie, N. ; Hassan, B. A. ; Bermingham, N. A. ; Malicki, D. M. ; Armstrong, D. ; Matzuk, M. ; Bellen, H. J. ; Zoghbi, H. 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Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional conservation of atonal and Math1 in the CNS and PNS</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>127</volume><issue>5</issue><spage>1039</spage><epage>1048</epage><pages>1039-1048</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>To determine the extent to which atonal and its mouse homolog Math1 exhibit functional conservation, we inserted (beta)-galactosidase (lacZ) into the Math1 locus and analyzed its expression, evaluated consequences of loss of Math1 function, and expressed Math1 in atonal mutant flies. lacZ under the control of Math1 regulatory elements duplicated the previously known expression pattern of Math1 in the CNS (i.e., the neural tube, dorsal spinal cord, brainstem, and cerebellar external granule neurons) but also revealed new sites of expression: PNS mechanoreceptors (inner ear hair cells and Merkel cells) and articular chondrocytes. Expressing Math1 induced ectopic chordotonal organs (CHOs) in wild-type flies and partially rescued CHO loss in atonal mutant embryos. These data demonstrate that both the mouse and fly homologs encode lineage identity information and, more interestingly, that some of the cells dependent on this information serve similar mechanoreceptor functions.</abstract><cop>Legacy CDMS</cop><pub>The Company of Biologists Limited</pub><pmid>10662643</pmid><doi>10.1242/dev.127.5.1039</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 2000-03, Vol.127 (5), p.1039-1048 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | MEDLINE; NASA Technical Reports Server; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals atonal gene Basic Helix-Loop-Helix Transcription Factors beta-Galactosidase - analysis beta-Galactosidase - genetics Central Nervous System - embryology DNA-Binding Proteins - genetics Drosophila Drosophila - embryology Drosophila Proteins Embryo, Nonmammalian - embryology Female Gene Expression Regulation, Developmental Helix-Loop-Helix Motifs lacZ gene Life Sciences (General) Male Math1 gene Mice Mice, Transgenic Nerve Tissue Proteins - genetics Organ Specificity Peripheral Nervous System - embryology Transcription Factors - genetics |
| title | Functional conservation of atonal and Math1 in the CNS and PNS |
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