A Functional Interaction between Dorsal and Components of the Smt3 Conjugation Machinery
To identify proteins that regulate the function of Dorsal, a Drosophila Rel family transcription factor, we employed a yeast two-hybrid screen to search for genes encoding Dorsal-interacting proteins. Six genes were identified, including two that encode previously known Dorsal-interacting proteins (...
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| Veröffentlicht in: | The Journal of biological chemistry 2000-02, Vol.275 (6), p.4033-4040 |
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| creator | Bhaskar, Vinay Valentine, Scott A. Courey, Albert J. |
| description | To identify proteins that regulate the function of Dorsal, a Drosophila Rel family transcription factor, we employed a yeast two-hybrid screen to search for genes encoding Dorsal-interacting proteins. Six genes were identified, including two that encode previously known Dorsal-interacting proteins (Twist and Cactus), three that encode novel proteins, and one that encodesDrosophila Ubc9 (DmUbc9), a protein thought to conjugate the ubiquitin-like polypeptide Smt3 to protein substrates. We have found that DmUbc9 binds and conjugates Drosophila Smt3 (DmSmt3) to Dorsal. In cultured cells, DmUbc9 was found to relieve inhibition of Dorsal nuclear uptake by Cactus, allowing Dorsal to enter the nucleus and activate transcription. The effect of DmUbc9 on Dorsal activity was potentiated by the overexpression of DmSmt3. We have also identified a DmSmt3-activating enzyme, DmSAE1/DmSAE2 and found that it further potentiates Dorsal-mediated activation. |
| doi_str_mv | 10.1074/jbc.275.6.4033 |
| format | Article |
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Six genes were identified, including two that encode previously known Dorsal-interacting proteins (Twist and Cactus), three that encode novel proteins, and one that encodesDrosophila Ubc9 (DmUbc9), a protein thought to conjugate the ubiquitin-like polypeptide Smt3 to protein substrates. We have found that DmUbc9 binds and conjugates Drosophila Smt3 (DmSmt3) to Dorsal. In cultured cells, DmUbc9 was found to relieve inhibition of Dorsal nuclear uptake by Cactus, allowing Dorsal to enter the nucleus and activate transcription. The effect of DmUbc9 on Dorsal activity was potentiated by the overexpression of DmSmt3. We have also identified a DmSmt3-activating enzyme, DmSAE1/DmSAE2 and found that it further potentiates Dorsal-mediated activation.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.275.6.4033</identifier><identifier>PMID: 10660560</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; amino acid sequences ; animal proteins ; Animals ; binding ; binding proteins ; Body Patterning ; Cactus protein ; Cell Line ; Cell Nucleus - metabolism ; Cloning, Molecular ; complementary DNA ; DmSAE1/DmSAE2 protein ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Dorsal protein ; Dorsal-interacting protein ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - embryology ; Drosophila Proteins ; genbank/af218858 ; genbank/af218859 ; genbank/af218860 ; genbank/af218861 ; genbank/af218862 ; genbank/af218863 ; genbank/af218864 ; Gene Expression Regulation, Developmental ; Green Fluorescent Proteins ; Insect Proteins - genetics ; Insect Proteins - metabolism ; interactions ; Ligases - chemistry ; Ligases - genetics ; luciferase ; Luminescent Proteins ; Microscopy, Fluorescence ; Molecular Sequence Data ; Nuclear Proteins - analysis ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; nuclei ; nucleotide sequences ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; protein transport ; reporter genes ; Sequence Homology, Amino Acid ; Smt3 protein ; transcription (genetics) ; transcription factors ; Transcription Factors - metabolism ; transcriptional activation ; Twist protein ; Ubc9 protein ; Ubiquitin-Activating Enzymes ; Ubiquitin-Conjugating Enzymes ; Ubiquitins - genetics ; Ubiquitins - metabolism ; Yeasts</subject><ispartof>The Journal of biological chemistry, 2000-02, Vol.275 (6), p.4033-4040</ispartof><rights>2000 © 2000 ASBMB. 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Six genes were identified, including two that encode previously known Dorsal-interacting proteins (Twist and Cactus), three that encode novel proteins, and one that encodesDrosophila Ubc9 (DmUbc9), a protein thought to conjugate the ubiquitin-like polypeptide Smt3 to protein substrates. We have found that DmUbc9 binds and conjugates Drosophila Smt3 (DmSmt3) to Dorsal. In cultured cells, DmUbc9 was found to relieve inhibition of Dorsal nuclear uptake by Cactus, allowing Dorsal to enter the nucleus and activate transcription. The effect of DmUbc9 on Dorsal activity was potentiated by the overexpression of DmSmt3. We have also identified a DmSmt3-activating enzyme, DmSAE1/DmSAE2 and found that it further potentiates Dorsal-mediated activation.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>animal proteins</subject><subject>Animals</subject><subject>binding</subject><subject>binding proteins</subject><subject>Body Patterning</subject><subject>Cactus protein</subject><subject>Cell Line</subject><subject>Cell Nucleus - metabolism</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>DmSAE1/DmSAE2 protein</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Dorsal protein</subject><subject>Dorsal-interacting protein</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - embryology</subject><subject>Drosophila Proteins</subject><subject>genbank/af218858</subject><subject>genbank/af218859</subject><subject>genbank/af218860</subject><subject>genbank/af218861</subject><subject>genbank/af218862</subject><subject>genbank/af218863</subject><subject>genbank/af218864</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Green Fluorescent Proteins</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>interactions</subject><subject>Ligases - chemistry</subject><subject>Ligases - genetics</subject><subject>luciferase</subject><subject>Luminescent Proteins</subject><subject>Microscopy, Fluorescence</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - analysis</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>nuclei</subject><subject>nucleotide sequences</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - metabolism</subject><subject>protein transport</subject><subject>reporter genes</subject><subject>Sequence Homology, Amino Acid</subject><subject>Smt3 protein</subject><subject>transcription (genetics)</subject><subject>transcription factors</subject><subject>Transcription Factors - metabolism</subject><subject>transcriptional activation</subject><subject>Twist protein</subject><subject>Ubc9 protein</subject><subject>Ubiquitin-Activating Enzymes</subject><subject>Ubiquitin-Conjugating Enzymes</subject><subject>Ubiquitins - genetics</subject><subject>Ubiquitins - metabolism</subject><subject>Yeasts</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq2qqCy01x7bqAduSf0VJz6ibWmRQBwoEjfLdiYbrzb21k5A_Pt6CYf2UOGDrRk_82r0IPSR4Irghn_dGlvRpq5ExTFjb9CK4JaVrCb3b9EKY0pKSev2GJ2ktMX5cEneoWOChcC1wCt0f15czN5OLni9Ky79BFE_V4WB6RHAF99CTPlL-65Yh3EfPPgpFaEvpgGK23Fiue2380Y_T11rOzgP8ek9Our1LsGHl_cU3V18_7X-WV7d_Lhcn1-Vlgs6lZpb4KQmwkipeVczwzjky0KTd6UWt7QnjTGGGNxRampsRAuiMxQbKmXHTtHZkruP4fcMaVKjSxZ2O-0hzEk1uJVCSP4qSBouGGnrDFYLaGNIKUKv9tGNOj4pgtVBusrSVZauhDpIzwOfXpJnM0L3F75YzsCXBRjcZnh0EZRxwQ4w_pvyeYF6HZTeRJfU3S3FhGEqeS3oIaZdCMg-HxxElawDb6HLkXZSXXD_W_EPWvWkew</recordid><startdate>20000211</startdate><enddate>20000211</enddate><creator>Bhaskar, Vinay</creator><creator>Valentine, Scott A.</creator><creator>Courey, Albert J.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000211</creationdate><title>A Functional Interaction between Dorsal and Components of the Smt3 Conjugation Machinery</title><author>Bhaskar, Vinay ; Valentine, Scott A. ; Courey, Albert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-a4ce41516b99a4d53b34e3b3ce70492c082f17bbb1b0d22b50b68e6db20b299d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>animal proteins</topic><topic>Animals</topic><topic>binding</topic><topic>binding proteins</topic><topic>Body Patterning</topic><topic>Cactus protein</topic><topic>Cell Line</topic><topic>Cell Nucleus - metabolism</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>DmSAE1/DmSAE2 protein</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Dorsal protein</topic><topic>Dorsal-interacting protein</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - embryology</topic><topic>Drosophila Proteins</topic><topic>genbank/af218858</topic><topic>genbank/af218859</topic><topic>genbank/af218860</topic><topic>genbank/af218861</topic><topic>genbank/af218862</topic><topic>genbank/af218863</topic><topic>genbank/af218864</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Green Fluorescent Proteins</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>interactions</topic><topic>Ligases - chemistry</topic><topic>Ligases - genetics</topic><topic>luciferase</topic><topic>Luminescent Proteins</topic><topic>Microscopy, Fluorescence</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - analysis</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>nuclei</topic><topic>nucleotide sequences</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - metabolism</topic><topic>protein transport</topic><topic>reporter genes</topic><topic>Sequence Homology, Amino Acid</topic><topic>Smt3 protein</topic><topic>transcription (genetics)</topic><topic>transcription factors</topic><topic>Transcription Factors - metabolism</topic><topic>transcriptional activation</topic><topic>Twist protein</topic><topic>Ubc9 protein</topic><topic>Ubiquitin-Activating Enzymes</topic><topic>Ubiquitin-Conjugating Enzymes</topic><topic>Ubiquitins - genetics</topic><topic>Ubiquitins - metabolism</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhaskar, Vinay</creatorcontrib><creatorcontrib>Valentine, Scott A.</creatorcontrib><creatorcontrib>Courey, Albert J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhaskar, Vinay</au><au>Valentine, Scott A.</au><au>Courey, Albert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Functional Interaction between Dorsal and Components of the Smt3 Conjugation Machinery</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2000-02-11</date><risdate>2000</risdate><volume>275</volume><issue>6</issue><spage>4033</spage><epage>4040</epage><pages>4033-4040</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>To identify proteins that regulate the function of Dorsal, a Drosophila Rel family transcription factor, we employed a yeast two-hybrid screen to search for genes encoding Dorsal-interacting proteins. Six genes were identified, including two that encode previously known Dorsal-interacting proteins (Twist and Cactus), three that encode novel proteins, and one that encodesDrosophila Ubc9 (DmUbc9), a protein thought to conjugate the ubiquitin-like polypeptide Smt3 to protein substrates. We have found that DmUbc9 binds and conjugates Drosophila Smt3 (DmSmt3) to Dorsal. In cultured cells, DmUbc9 was found to relieve inhibition of Dorsal nuclear uptake by Cactus, allowing Dorsal to enter the nucleus and activate transcription. The effect of DmUbc9 on Dorsal activity was potentiated by the overexpression of DmSmt3. We have also identified a DmSmt3-activating enzyme, DmSAE1/DmSAE2 and found that it further potentiates Dorsal-mediated activation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10660560</pmid><doi>10.1074/jbc.275.6.4033</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence amino acid sequences animal proteins Animals binding binding proteins Body Patterning Cactus protein Cell Line Cell Nucleus - metabolism Cloning, Molecular complementary DNA DmSAE1/DmSAE2 protein DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Dorsal protein Dorsal-interacting protein Drosophila Drosophila melanogaster Drosophila melanogaster - embryology Drosophila Proteins genbank/af218858 genbank/af218859 genbank/af218860 genbank/af218861 genbank/af218862 genbank/af218863 genbank/af218864 Gene Expression Regulation, Developmental Green Fluorescent Proteins Insect Proteins - genetics Insect Proteins - metabolism interactions Ligases - chemistry Ligases - genetics luciferase Luminescent Proteins Microscopy, Fluorescence Molecular Sequence Data Nuclear Proteins - analysis Nuclear Proteins - genetics Nuclear Proteins - metabolism nuclei nucleotide sequences Phosphoproteins - genetics Phosphoproteins - metabolism protein transport reporter genes Sequence Homology, Amino Acid Smt3 protein transcription (genetics) transcription factors Transcription Factors - metabolism transcriptional activation Twist protein Ubc9 protein Ubiquitin-Activating Enzymes Ubiquitin-Conjugating Enzymes Ubiquitins - genetics Ubiquitins - metabolism Yeasts |
| title | A Functional Interaction between Dorsal and Components of the Smt3 Conjugation Machinery |
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