Dominant T cells in idiopathic nephrotic syndrome of childhood
Dominant T cells in idiopathic nephrotic syndrome of childhood. Because of several studies, idiopathic nephrotic syndrome (INS) of childhood is suspected to have an immunologic pathogenesis with T cells playing a major role. To investigate this hypothesis further, we studied the diversity of the CDR...
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| Veröffentlicht in: | Kidney international 2000-02, Vol.57 (2), p.510-517 |
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| creator | Frank, Carola Herrmann, Martin Fernandez, Stefany Dirnecker, Diemuth Böswald, Michael Kolowos, Wasilis Ruder, Hans Haas, Johannes-Peter |
| description | Dominant T cells in idiopathic nephrotic syndrome of childhood.
Because of several studies, idiopathic nephrotic syndrome (INS) of childhood is suspected to have an immunologic pathogenesis with T cells playing a major role. To investigate this hypothesis further, we studied the diversity of the CDR3 region of the T-cell receptor (TCR) β;-chain from peripheral T cells isolated from patients with INS.
The study was performed over a three-year period to obtain longitudinal data on the repertoire of peripheral T cells. mRNA from peripheral mononuclear cells (PBMCs) of seven INS patients and two healthy controls (NHD) was prepared and analyzed for CDR3 length polymorphism of TCR β;-chain by spectratyping.
All INS patients presented individually skewed spectratype histograms in at least one Vβ;-family. Patients suffering from a frequent relapsing course of INS or a focal global sclerosis showed some alterations to persist in all samples isolated in the observation period (up to 3 years). In addition, sequence analyses of the β;-chain of the TCR CDR3 region confirmed clonal expansion of peripheral T cells in those patients who had displayed spectratype alterations.
The data give strong evidence for an direct involvement of CD8+ T cells in the complicated course of INS. |
| doi_str_mv | 10.1046/j.1523-1755.2000.00870.x |
| format | Article |
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Because of several studies, idiopathic nephrotic syndrome (INS) of childhood is suspected to have an immunologic pathogenesis with T cells playing a major role. To investigate this hypothesis further, we studied the diversity of the CDR3 region of the T-cell receptor (TCR) β;-chain from peripheral T cells isolated from patients with INS.
The study was performed over a three-year period to obtain longitudinal data on the repertoire of peripheral T cells. mRNA from peripheral mononuclear cells (PBMCs) of seven INS patients and two healthy controls (NHD) was prepared and analyzed for CDR3 length polymorphism of TCR β;-chain by spectratyping.
All INS patients presented individually skewed spectratype histograms in at least one Vβ;-family. Patients suffering from a frequent relapsing course of INS or a focal global sclerosis showed some alterations to persist in all samples isolated in the observation period (up to 3 years). In addition, sequence analyses of the β;-chain of the TCR CDR3 region confirmed clonal expansion of peripheral T cells in those patients who had displayed spectratype alterations.
The data give strong evidence for an direct involvement of CD8+ T cells in the complicated course of INS.</description><identifier>ISSN: 0085-2538</identifier><identifier>EISSN: 1523-1755</identifier><identifier>DOI: 10.1046/j.1523-1755.2000.00870.x</identifier><identifier>PMID: 10652027</identifier><identifier>CODEN: KDYIA5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Age of Onset ; AIDS/HIV ; Amino Acid Sequence ; Biological and medical sciences ; CD4-Positive T-Lymphocytes - chemistry ; CD4-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - chemistry ; CD8-Positive T-Lymphocytes - immunology ; CDR3 length polymorphism ; Child ; childhood nephrotic syndrome ; Complementarity Determining Regions ; focal global sclerosis ; Gene Expression - immunology ; Genes, T-Cell Receptor beta - genetics ; Genes, T-Cell Receptor beta - immunology ; Glomerulonephritis ; Humans ; immune system ; Immunoglobulin Variable Region - genetics ; Medical sciences ; Molecular Sequence Data ; Nephrology. Urinary tract diseases ; Nephropathies. Renovascular diseases. Renal failure ; Nephrotic Syndrome - etiology ; Nephrotic Syndrome - immunology ; Nephrotic Syndrome - physiopathology ; Polymorphism, Genetic ; Receptors, Antigen, T-Cell, alpha-beta - chemistry ; Receptors, Antigen, T-Cell, alpha-beta - genetics ; Receptors, Antigen, T-Cell, alpha-beta - immunology ; Sequence Analysis, DNA ; T cell receptor ; T-Lymphocyte Subsets - immunology</subject><ispartof>Kidney international, 2000-02, Vol.57 (2), p.510-517</ispartof><rights>2000 International Society of Nephrology</rights><rights>2000 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Feb 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-5558adf33560255b14279a3479bc997a934fdb02445e4d68da4e213386f1ca613</citedby><cites>FETCH-LOGICAL-c498t-5558adf33560255b14279a3479bc997a934fdb02445e4d68da4e213386f1ca613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/210108726?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1288418$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10652027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frank, Carola</creatorcontrib><creatorcontrib>Herrmann, Martin</creatorcontrib><creatorcontrib>Fernandez, Stefany</creatorcontrib><creatorcontrib>Dirnecker, Diemuth</creatorcontrib><creatorcontrib>Böswald, Michael</creatorcontrib><creatorcontrib>Kolowos, Wasilis</creatorcontrib><creatorcontrib>Ruder, Hans</creatorcontrib><creatorcontrib>Haas, Johannes-Peter</creatorcontrib><title>Dominant T cells in idiopathic nephrotic syndrome of childhood</title><title>Kidney international</title><addtitle>Kidney Int</addtitle><description>Dominant T cells in idiopathic nephrotic syndrome of childhood.
Because of several studies, idiopathic nephrotic syndrome (INS) of childhood is suspected to have an immunologic pathogenesis with T cells playing a major role. To investigate this hypothesis further, we studied the diversity of the CDR3 region of the T-cell receptor (TCR) β;-chain from peripheral T cells isolated from patients with INS.
The study was performed over a three-year period to obtain longitudinal data on the repertoire of peripheral T cells. mRNA from peripheral mononuclear cells (PBMCs) of seven INS patients and two healthy controls (NHD) was prepared and analyzed for CDR3 length polymorphism of TCR β;-chain by spectratyping.
All INS patients presented individually skewed spectratype histograms in at least one Vβ;-family. Patients suffering from a frequent relapsing course of INS or a focal global sclerosis showed some alterations to persist in all samples isolated in the observation period (up to 3 years). In addition, sequence analyses of the β;-chain of the TCR CDR3 region confirmed clonal expansion of peripheral T cells in those patients who had displayed spectratype alterations.
The data give strong evidence for an direct involvement of CD8+ T cells in the complicated course of INS.</description><subject>Age of Onset</subject><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>CD4-Positive T-Lymphocytes - chemistry</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - chemistry</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CDR3 length polymorphism</subject><subject>Child</subject><subject>childhood nephrotic syndrome</subject><subject>Complementarity Determining Regions</subject><subject>focal global sclerosis</subject><subject>Gene Expression - immunology</subject><subject>Genes, T-Cell Receptor beta - genetics</subject><subject>Genes, T-Cell Receptor beta - immunology</subject><subject>Glomerulonephritis</subject><subject>Humans</subject><subject>immune system</subject><subject>Immunoglobulin Variable Region - genetics</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Nephropathies. Renovascular diseases. Renal failure</subject><subject>Nephrotic Syndrome - etiology</subject><subject>Nephrotic Syndrome - immunology</subject><subject>Nephrotic Syndrome - physiopathology</subject><subject>Polymorphism, Genetic</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - chemistry</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - genetics</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - immunology</subject><subject>Sequence Analysis, DNA</subject><subject>T cell receptor</subject><subject>T-Lymphocyte Subsets - immunology</subject><issn>0085-2538</issn><issn>1523-1755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkEtLxDAYRYMoOj5-glJE3LXm2aYbwbeC4EbXIZOkTIY2GZNW9N-b2kHFjauE3PN93BwAMgQLBGl5tiwQwyRHFWMFhhAWEPIKFu8bYPYdbIJZemU5ZoTvgN0YlwnkNYHbYAfBkmGIqxk4v_adddL12XOmTNvGzLrMautXsl9YlTmzWgTfp1v8cDr4zmS-ydTCtnrhvd4HW41sozlYn3vg5fbm-eo-f3y6e7i6eMwVrXmfM8a41A0hrISYsTmiuKoloVU9V3VdyZrQRs8hppQZqkuuJTUYEcLLBilZIrIHTqe9q-BfBxN70dk49pXO-CGKKv2M8WoEj_-ASz8El7oJjCBKmnCZID5BKvgYg2nEKthOhg-BoBgFi6UYPYrRoxgFiy_B4j2NHq33D_PO6F-Dk9EEnKwBGZVsmyCdsvGHw5xTxBN2OGFO9kMw3zmlNUqWUn455SZZfbMmiKisccpoG4zqhfb2_7KfnRWgiQ</recordid><startdate>20000201</startdate><enddate>20000201</enddate><creator>Frank, Carola</creator><creator>Herrmann, Martin</creator><creator>Fernandez, Stefany</creator><creator>Dirnecker, Diemuth</creator><creator>Böswald, Michael</creator><creator>Kolowos, Wasilis</creator><creator>Ruder, Hans</creator><creator>Haas, Johannes-Peter</creator><general>Elsevier Inc</general><general>Nature Publishing</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20000201</creationdate><title>Dominant T cells in idiopathic nephrotic syndrome of childhood</title><author>Frank, Carola ; Herrmann, Martin ; Fernandez, Stefany ; Dirnecker, Diemuth ; Böswald, Michael ; Kolowos, Wasilis ; Ruder, Hans ; Haas, Johannes-Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-5558adf33560255b14279a3479bc997a934fdb02445e4d68da4e213386f1ca613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Age of Onset</topic><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>CD4-Positive T-Lymphocytes - chemistry</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - chemistry</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>CDR3 length polymorphism</topic><topic>Child</topic><topic>childhood nephrotic syndrome</topic><topic>Complementarity Determining Regions</topic><topic>focal global sclerosis</topic><topic>Gene Expression - immunology</topic><topic>Genes, T-Cell Receptor beta - genetics</topic><topic>Genes, T-Cell Receptor beta - immunology</topic><topic>Glomerulonephritis</topic><topic>Humans</topic><topic>immune system</topic><topic>Immunoglobulin Variable Region - genetics</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Nephropathies. Renovascular diseases. Renal failure</topic><topic>Nephrotic Syndrome - etiology</topic><topic>Nephrotic Syndrome - immunology</topic><topic>Nephrotic Syndrome - physiopathology</topic><topic>Polymorphism, Genetic</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - chemistry</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - genetics</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - immunology</topic><topic>Sequence Analysis, DNA</topic><topic>T cell receptor</topic><topic>T-Lymphocyte Subsets - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frank, Carola</creatorcontrib><creatorcontrib>Herrmann, Martin</creatorcontrib><creatorcontrib>Fernandez, Stefany</creatorcontrib><creatorcontrib>Dirnecker, Diemuth</creatorcontrib><creatorcontrib>Böswald, Michael</creatorcontrib><creatorcontrib>Kolowos, Wasilis</creatorcontrib><creatorcontrib>Ruder, Hans</creatorcontrib><creatorcontrib>Haas, Johannes-Peter</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Kidney international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frank, Carola</au><au>Herrmann, Martin</au><au>Fernandez, Stefany</au><au>Dirnecker, Diemuth</au><au>Böswald, Michael</au><au>Kolowos, Wasilis</au><au>Ruder, Hans</au><au>Haas, Johannes-Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dominant T cells in idiopathic nephrotic syndrome of childhood</atitle><jtitle>Kidney international</jtitle><addtitle>Kidney Int</addtitle><date>2000-02-01</date><risdate>2000</risdate><volume>57</volume><issue>2</issue><spage>510</spage><epage>517</epage><pages>510-517</pages><issn>0085-2538</issn><eissn>1523-1755</eissn><coden>KDYIA5</coden><abstract>Dominant T cells in idiopathic nephrotic syndrome of childhood.
Because of several studies, idiopathic nephrotic syndrome (INS) of childhood is suspected to have an immunologic pathogenesis with T cells playing a major role. To investigate this hypothesis further, we studied the diversity of the CDR3 region of the T-cell receptor (TCR) β;-chain from peripheral T cells isolated from patients with INS.
The study was performed over a three-year period to obtain longitudinal data on the repertoire of peripheral T cells. mRNA from peripheral mononuclear cells (PBMCs) of seven INS patients and two healthy controls (NHD) was prepared and analyzed for CDR3 length polymorphism of TCR β;-chain by spectratyping.
All INS patients presented individually skewed spectratype histograms in at least one Vβ;-family. Patients suffering from a frequent relapsing course of INS or a focal global sclerosis showed some alterations to persist in all samples isolated in the observation period (up to 3 years). In addition, sequence analyses of the β;-chain of the TCR CDR3 region confirmed clonal expansion of peripheral T cells in those patients who had displayed spectratype alterations.
The data give strong evidence for an direct involvement of CD8+ T cells in the complicated course of INS.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10652027</pmid><doi>10.1046/j.1523-1755.2000.00870.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Age of Onset AIDS/HIV Amino Acid Sequence Biological and medical sciences CD4-Positive T-Lymphocytes - chemistry CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - chemistry CD8-Positive T-Lymphocytes - immunology CDR3 length polymorphism Child childhood nephrotic syndrome Complementarity Determining Regions focal global sclerosis Gene Expression - immunology Genes, T-Cell Receptor beta - genetics Genes, T-Cell Receptor beta - immunology Glomerulonephritis Humans immune system Immunoglobulin Variable Region - genetics Medical sciences Molecular Sequence Data Nephrology. Urinary tract diseases Nephropathies. Renovascular diseases. Renal failure Nephrotic Syndrome - etiology Nephrotic Syndrome - immunology Nephrotic Syndrome - physiopathology Polymorphism, Genetic Receptors, Antigen, T-Cell, alpha-beta - chemistry Receptors, Antigen, T-Cell, alpha-beta - genetics Receptors, Antigen, T-Cell, alpha-beta - immunology Sequence Analysis, DNA T cell receptor T-Lymphocyte Subsets - immunology |
| title | Dominant T cells in idiopathic nephrotic syndrome of childhood |
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