Effects of Thyroid Hormones on Bovine Granulosa and Thecal Cell Function In Vitro: Dependence on Insulin and Gonadotropins
The objective of this study was to evaluate the influence of thyroxine (T4) and triiodothryonine (T3) on steroid production by bovine granulosa and thecal cells. Granulosa and thecal cells were obtained from small (1 to 5 mm) and large (> or = 8 mm) follicles of cattle, respectively, and cultured...
Gespeichert in:
| Veröffentlicht in: | Journal of dairy science 2001-05, Vol.84 (5), p.1069-1076 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1076 |
|---|---|
| container_issue | 5 |
| container_start_page | 1069 |
| container_title | Journal of dairy science |
| container_volume | 84 |
| creator | Spicer, L. J Alonso, J Chamberlain, C. S |
| description | The objective of this study was to evaluate the influence of thyroxine (T4) and triiodothryonine (T3) on steroid production by bovine granulosa and thecal cells. Granulosa and thecal cells were obtained from small (1 to 5 mm) and large (> or = 8 mm) follicles of cattle, respectively, and cultured for 4 d. We conducted six experiments to evaluate the effect of 2 d of exposure to various doses of T3 or T4. In insulin- or insulin plus FSH-treated granulosa cells of experiment 1, 30 and 100 ng/ml of T4 had no effect on aromatase activity or progesterone production. In experiment 2, in the presence of insulin and FSH, 1 and 3 ng/ml of T3 weakly ( |
| doi_str_mv | 10.3168/jds.s0022-0302(01)74567-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70894272</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70894272</sourcerecordid><originalsourceid>FETCH-LOGICAL-c490t-1d0c187206dd524572560d53998f9b65f803558350798504fc545eb22611dc7d3</originalsourceid><addsrcrecordid>eNpNkNFuFCEUhomxsWv1FQwmxujFtAcYBvBO13bbpEkvrN4SFhiXZgZWmNHUp5fdbmyvCCfff_6TD6G3BE4Z6eTZnSunBYDSBhjQD0A-ipZ3ouHP0IJwyhtGlHyOFv-RY_SylLv6JRT4C3RMCJMtMLZAf8_73tup4NTj2819TsHhy5THFH2dRfwl_Q7R41U2cR5SMdhEV0FvzYCXfhjwxRztFCp5FfGPMOX0CX_1Wx-dj9bj_bzMQ4j74CpF41KFtiGWV-ioN0Pxrw_vCfp-cX67vGyub1ZXy8_XjW0VTA1xYIkUFDrnOG25oLwDx5lSslfrjvcSGOeScRBKcmh7y1vu15R2hDgrHDtB7x_2bnP6Nfsy6TEUW2830ae5aAFStVTQCqoH0OZUSva93uYwmnyvCeideF3F6287q3pnVQPRe_Ga1-ybQ8m8Hr17TB5MV-DdATClyuurUBvKkwZKmYDHYzfh5-ZPyF6X0QxD3Up27bLVvLKdYv8A6syYyQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70894272</pqid></control><display><type>article</type><title>Effects of Thyroid Hormones on Bovine Granulosa and Thecal Cell Function In Vitro: Dependence on Insulin and Gonadotropins</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Spicer, L. J ; Alonso, J ; Chamberlain, C. S</creator><creatorcontrib>Spicer, L. J ; Alonso, J ; Chamberlain, C. S</creatorcontrib><description>The objective of this study was to evaluate the influence of thyroxine (T4) and triiodothryonine (T3) on steroid production by bovine granulosa and thecal cells. Granulosa and thecal cells were obtained from small (1 to 5 mm) and large (> or = 8 mm) follicles of cattle, respectively, and cultured for 4 d. We conducted six experiments to evaluate the effect of 2 d of exposure to various doses of T3 or T4. In insulin- or insulin plus FSH-treated granulosa cells of experiment 1, 30 and 100 ng/ml of T4 had no effect on aromatase activity or progesterone production. In experiment 2, in the presence of insulin and FSH, 1 and 3 ng/ml of T3 weakly (<1.4-fold) increased aromatase activity of granulosa cells but had no effect on progesterone production. Low doses of T4 (3 to 30 ng/ml) tested in experiment 3 had no effect on aromatase activity but increased (to as much as 1.4-fold) progesterone production by granulosa cells. In experiment 4, T4 (30 ng/ml) increased (to 1.2-fold) progesterone production by granulosa cells only in the presence of FSH and had no effect on aromatase activity. In thecal cells of experiment 5, in the presence of insulin and LH, 30 and 100 mg/ml of T4 increased androstenedione production to 2.3- and 2.8-fold, respectively; only 100 ng/ml of T4 was effective at stimulating progesterone production by thecal cells. In experiment 6, 1 ng/ml of T3 increased thecal cell androstenedione production to 3.9-fold, whereas 3 ng/ml of T3 was without effect; progesterone production was not affected by T3. These results support the hypothesis that thyroid hormones may have direct stimulatory effects on ovarian function in cattle, acting at the level of granulosa and thecal cells.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.s0022-0302(01)74567-5</identifier><identifier>PMID: 11384033</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Am Dairy Sci Assoc</publisher><subject>Animal productions ; Animals ; Aromatase - metabolism ; Biological and medical sciences ; Cattle ; Cells, Cultured ; Culture Media ; Female ; Fundamental and applied biological sciences. Psychology ; Gonadotropins - pharmacology ; Granulosa Cells - drug effects ; Granulosa Cells - metabolism ; Hormone metabolism and regulation ; Insulin - pharmacology ; Mammalian female genital system ; Ovarian Follicle - cytology ; Progesterone - metabolism ; Radioimmunoassay ; Terrestrial animal productions ; Theca Cells - drug effects ; Theca Cells - metabolism ; Thyroxine - pharmacology ; Triiodothyronine - pharmacology ; Vertebrates ; Vertebrates: reproduction</subject><ispartof>Journal of dairy science, 2001-05, Vol.84 (5), p.1069-1076</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-1d0c187206dd524572560d53998f9b65f803558350798504fc545eb22611dc7d3</citedby><cites>FETCH-LOGICAL-c490t-1d0c187206dd524572560d53998f9b65f803558350798504fc545eb22611dc7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1022370$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11384033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spicer, L. J</creatorcontrib><creatorcontrib>Alonso, J</creatorcontrib><creatorcontrib>Chamberlain, C. S</creatorcontrib><title>Effects of Thyroid Hormones on Bovine Granulosa and Thecal Cell Function In Vitro: Dependence on Insulin and Gonadotropins</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>The objective of this study was to evaluate the influence of thyroxine (T4) and triiodothryonine (T3) on steroid production by bovine granulosa and thecal cells. Granulosa and thecal cells were obtained from small (1 to 5 mm) and large (> or = 8 mm) follicles of cattle, respectively, and cultured for 4 d. We conducted six experiments to evaluate the effect of 2 d of exposure to various doses of T3 or T4. In insulin- or insulin plus FSH-treated granulosa cells of experiment 1, 30 and 100 ng/ml of T4 had no effect on aromatase activity or progesterone production. In experiment 2, in the presence of insulin and FSH, 1 and 3 ng/ml of T3 weakly (<1.4-fold) increased aromatase activity of granulosa cells but had no effect on progesterone production. Low doses of T4 (3 to 30 ng/ml) tested in experiment 3 had no effect on aromatase activity but increased (to as much as 1.4-fold) progesterone production by granulosa cells. In experiment 4, T4 (30 ng/ml) increased (to 1.2-fold) progesterone production by granulosa cells only in the presence of FSH and had no effect on aromatase activity. In thecal cells of experiment 5, in the presence of insulin and LH, 30 and 100 mg/ml of T4 increased androstenedione production to 2.3- and 2.8-fold, respectively; only 100 ng/ml of T4 was effective at stimulating progesterone production by thecal cells. In experiment 6, 1 ng/ml of T3 increased thecal cell androstenedione production to 3.9-fold, whereas 3 ng/ml of T3 was without effect; progesterone production was not affected by T3. These results support the hypothesis that thyroid hormones may have direct stimulatory effects on ovarian function in cattle, acting at the level of granulosa and thecal cells.</description><subject>Animal productions</subject><subject>Animals</subject><subject>Aromatase - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gonadotropins - pharmacology</subject><subject>Granulosa Cells - drug effects</subject><subject>Granulosa Cells - metabolism</subject><subject>Hormone metabolism and regulation</subject><subject>Insulin - pharmacology</subject><subject>Mammalian female genital system</subject><subject>Ovarian Follicle - cytology</subject><subject>Progesterone - metabolism</subject><subject>Radioimmunoassay</subject><subject>Terrestrial animal productions</subject><subject>Theca Cells - drug effects</subject><subject>Theca Cells - metabolism</subject><subject>Thyroxine - pharmacology</subject><subject>Triiodothyronine - pharmacology</subject><subject>Vertebrates</subject><subject>Vertebrates: reproduction</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkNFuFCEUhomxsWv1FQwmxujFtAcYBvBO13bbpEkvrN4SFhiXZgZWmNHUp5fdbmyvCCfff_6TD6G3BE4Z6eTZnSunBYDSBhjQD0A-ipZ3ouHP0IJwyhtGlHyOFv-RY_SylLv6JRT4C3RMCJMtMLZAf8_73tup4NTj2819TsHhy5THFH2dRfwl_Q7R41U2cR5SMdhEV0FvzYCXfhjwxRztFCp5FfGPMOX0CX_1Wx-dj9bj_bzMQ4j74CpF41KFtiGWV-ioN0Pxrw_vCfp-cX67vGyub1ZXy8_XjW0VTA1xYIkUFDrnOG25oLwDx5lSslfrjvcSGOeScRBKcmh7y1vu15R2hDgrHDtB7x_2bnP6Nfsy6TEUW2830ae5aAFStVTQCqoH0OZUSva93uYwmnyvCeideF3F6287q3pnVQPRe_Ga1-ybQ8m8Hr17TB5MV-DdATClyuurUBvKkwZKmYDHYzfh5-ZPyF6X0QxD3Up27bLVvLKdYv8A6syYyQ</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Spicer, L. J</creator><creator>Alonso, J</creator><creator>Chamberlain, C. S</creator><general>Am Dairy Sci Assoc</general><general>American Dairy Science Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Effects of Thyroid Hormones on Bovine Granulosa and Thecal Cell Function In Vitro: Dependence on Insulin and Gonadotropins</title><author>Spicer, L. J ; Alonso, J ; Chamberlain, C. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-1d0c187206dd524572560d53998f9b65f803558350798504fc545eb22611dc7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animal productions</topic><topic>Animals</topic><topic>Aromatase - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonadotropins - pharmacology</topic><topic>Granulosa Cells - drug effects</topic><topic>Granulosa Cells - metabolism</topic><topic>Hormone metabolism and regulation</topic><topic>Insulin - pharmacology</topic><topic>Mammalian female genital system</topic><topic>Ovarian Follicle - cytology</topic><topic>Progesterone - metabolism</topic><topic>Radioimmunoassay</topic><topic>Terrestrial animal productions</topic><topic>Theca Cells - drug effects</topic><topic>Theca Cells - metabolism</topic><topic>Thyroxine - pharmacology</topic><topic>Triiodothyronine - pharmacology</topic><topic>Vertebrates</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Spicer, L. J</creatorcontrib><creatorcontrib>Alonso, J</creatorcontrib><creatorcontrib>Chamberlain, C. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spicer, L. J</au><au>Alonso, J</au><au>Chamberlain, C. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Thyroid Hormones on Bovine Granulosa and Thecal Cell Function In Vitro: Dependence on Insulin and Gonadotropins</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>84</volume><issue>5</issue><spage>1069</spage><epage>1076</epage><pages>1069-1076</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><coden>JDSCAE</coden><abstract>The objective of this study was to evaluate the influence of thyroxine (T4) and triiodothryonine (T3) on steroid production by bovine granulosa and thecal cells. Granulosa and thecal cells were obtained from small (1 to 5 mm) and large (> or = 8 mm) follicles of cattle, respectively, and cultured for 4 d. We conducted six experiments to evaluate the effect of 2 d of exposure to various doses of T3 or T4. In insulin- or insulin plus FSH-treated granulosa cells of experiment 1, 30 and 100 ng/ml of T4 had no effect on aromatase activity or progesterone production. In experiment 2, in the presence of insulin and FSH, 1 and 3 ng/ml of T3 weakly (<1.4-fold) increased aromatase activity of granulosa cells but had no effect on progesterone production. Low doses of T4 (3 to 30 ng/ml) tested in experiment 3 had no effect on aromatase activity but increased (to as much as 1.4-fold) progesterone production by granulosa cells. In experiment 4, T4 (30 ng/ml) increased (to 1.2-fold) progesterone production by granulosa cells only in the presence of FSH and had no effect on aromatase activity. In thecal cells of experiment 5, in the presence of insulin and LH, 30 and 100 mg/ml of T4 increased androstenedione production to 2.3- and 2.8-fold, respectively; only 100 ng/ml of T4 was effective at stimulating progesterone production by thecal cells. In experiment 6, 1 ng/ml of T3 increased thecal cell androstenedione production to 3.9-fold, whereas 3 ng/ml of T3 was without effect; progesterone production was not affected by T3. These results support the hypothesis that thyroid hormones may have direct stimulatory effects on ovarian function in cattle, acting at the level of granulosa and thecal cells.</abstract><cop>Savoy, IL</cop><pub>Am Dairy Sci Assoc</pub><pmid>11384033</pmid><doi>10.3168/jds.s0022-0302(01)74567-5</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-0302 |
| ispartof | Journal of dairy science, 2001-05, Vol.84 (5), p.1069-1076 |
| issn | 0022-0302 1525-3198 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70894272 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals |
| subjects | Animal productions Animals Aromatase - metabolism Biological and medical sciences Cattle Cells, Cultured Culture Media Female Fundamental and applied biological sciences. Psychology Gonadotropins - pharmacology Granulosa Cells - drug effects Granulosa Cells - metabolism Hormone metabolism and regulation Insulin - pharmacology Mammalian female genital system Ovarian Follicle - cytology Progesterone - metabolism Radioimmunoassay Terrestrial animal productions Theca Cells - drug effects Theca Cells - metabolism Thyroxine - pharmacology Triiodothyronine - pharmacology Vertebrates Vertebrates: reproduction |
| title | Effects of Thyroid Hormones on Bovine Granulosa and Thecal Cell Function In Vitro: Dependence on Insulin and Gonadotropins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T04%3A41%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20Thyroid%20Hormones%20on%20Bovine%20Granulosa%20and%20Thecal%20Cell%20Function%20In%20Vitro:%20Dependence%20on%20Insulin%20and%20Gonadotropins&rft.jtitle=Journal%20of%20dairy%20science&rft.au=Spicer,%20L.%20J&rft.date=2001-05-01&rft.volume=84&rft.issue=5&rft.spage=1069&rft.epage=1076&rft.pages=1069-1076&rft.issn=0022-0302&rft.eissn=1525-3198&rft.coden=JDSCAE&rft_id=info:doi/10.3168/jds.s0022-0302(01)74567-5&rft_dat=%3Cproquest_cross%3E70894272%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70894272&rft_id=info:pmid/11384033&rfr_iscdi=true |