Enrichment of high-quality spermatozoa in bovine semen: relative effectiveness of three filtration matrixes
To be practical, any method for improving bull semen must yield a large quantity of motile spermatozoa. Some separation methods based on physical properties, e.g. filtration, chromatography, centrifugation, washing and pooling, have been reported as satisfactory, but generally are not repeatable. Ne...
Gespeichert in:
| Veröffentlicht in: | Andrologia 2001-05, Vol.33 (3), p.143-150 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 150 |
|---|---|
| container_issue | 3 |
| container_start_page | 143 |
| container_title | Andrologia |
| container_volume | 33 |
| creator | Cisale, H. O. Fischman, M. L. Blasi, C. D. Fernandez, H. A. Gledhill, B. L. |
| description | To be practical, any method for improving bull semen must yield a large quantity of motile spermatozoa. Some separation methods based on physical properties, e.g. filtration, chromatography, centrifugation, washing and pooling, have been reported as satisfactory, but generally are not repeatable. Nevertheless, filtration methods appear to allow the attainment of an acceptable number of spermatozoa, thus allowing such a technique to be introduced in the production of standard bovine semen doses for artificial insemination. The aim of this work was to evaluate systematically the relative effects of three filtration matrixes (silica oxide, glass beads or Sephadexä™) on the improvement of whole ejaculate quality. Analysis of the type of matrix and the volume and height of the filtration column was performed. The only characteristic of the columns that appears to influence ejaculate quality after filtering is the matrix volume. While all matrixes produced improvement of semen quality, SephadexTM was better than the other matrixes tested. An explanation for the mechanism of column filtration is proposed. |
| doi_str_mv | 10.1046/j.1439-0272.2001.00413.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_70890549</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70890549</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4323-4eb4b540608cdb72f38a8dae64d80c2d101a8b8e4de89960456c69c02c1606da3</originalsourceid><addsrcrecordid>eNpNkVtv1DAQhS0EoqvSv4D8gHhLGF_iOIiXqpQtoioSKoI3y3EmrLe5bO1s2eXX47BLYV5mpPnO0diHEMogZyDVm3XOpKgy4CXPOQDLASQT-e4JWTwunpIFCBCZLFR1Qs5iXEMqWZSllM_JCWNCg-DVgtxdDsG7VY_DRMeWrvyPVXa_tZ2f9jRuMPR2Gn-NlvqB1uODH5BGTPBbGrCzk39Aim2Lbp4GjHH2mFYBkba-m0IixoEmj-B3GF-QZ63tIp4d-yn5-uHy9uIqu_68_Hhxfp05KXi6GWtZFxIUaNfUJW-FtrqxqGSjwfGGAbO61igb1FWl0quUU5UD7pgC1VhxSl4ffDdhvN9inEzvo8OuswOO22hK0BUUskrgyyO4rXtszCb43oa9-fs9CXh1BGx0tmuDHZyP_zjQIHmRsHcH7KfvcP_f2syBmbWZczFzLmYOzPwJzOzM-c37NCR5dpD7OOHuUW7DnVGlKAvz7WZpviw_fRe3EsyV-A3-YJjI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70890549</pqid></control><display><type>article</type><title>Enrichment of high-quality spermatozoa in bovine semen: relative effectiveness of three filtration matrixes</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Cisale, H. O. ; Fischman, M. L. ; Blasi, C. D. ; Fernandez, H. A. ; Gledhill, B. L.</creator><creatorcontrib>Cisale, H. O. ; Fischman, M. L. ; Blasi, C. D. ; Fernandez, H. A. ; Gledhill, B. L.</creatorcontrib><description>To be practical, any method for improving bull semen must yield a large quantity of motile spermatozoa. Some separation methods based on physical properties, e.g. filtration, chromatography, centrifugation, washing and pooling, have been reported as satisfactory, but generally are not repeatable. Nevertheless, filtration methods appear to allow the attainment of an acceptable number of spermatozoa, thus allowing such a technique to be introduced in the production of standard bovine semen doses for artificial insemination. The aim of this work was to evaluate systematically the relative effects of three filtration matrixes (silica oxide, glass beads or Sephadexä™) on the improvement of whole ejaculate quality. Analysis of the type of matrix and the volume and height of the filtration column was performed. The only characteristic of the columns that appears to influence ejaculate quality after filtering is the matrix volume. While all matrixes produced improvement of semen quality, SephadexTM was better than the other matrixes tested. An explanation for the mechanism of column filtration is proposed.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1046/j.1439-0272.2001.00413.x</identifier><identifier>PMID: 11380329</identifier><identifier>CODEN: ANDRDQ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science, Ltd</publisher><subject>Acrosome - physiology ; Animals ; Biological and medical sciences ; Birth control ; Bull ; Cattle ; Cell Adhesion ; Cell Separation - methods ; Cell Size ; Coloring Agents ; column filtration ; Eosine Yellowish-(YS) ; Filtration ; Glass ; Gynecology. Andrology. Obstetrics ; Hypotonic Solutions ; Linear Models ; Male ; Medical sciences ; Microspheres ; Other methods of contraception. Sterilization ; semen quality ; Sperm Count ; Sperm Motility ; Spermatozoa - cytology ; Spermatozoa - physiology</subject><ispartof>Andrologia, 2001-05, Vol.33 (3), p.143-150</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4323-4eb4b540608cdb72f38a8dae64d80c2d101a8b8e4de89960456c69c02c1606da3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1439-0272.2001.00413.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1439-0272.2001.00413.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1080425$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11380329$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cisale, H. O.</creatorcontrib><creatorcontrib>Fischman, M. L.</creatorcontrib><creatorcontrib>Blasi, C. D.</creatorcontrib><creatorcontrib>Fernandez, H. A.</creatorcontrib><creatorcontrib>Gledhill, B. L.</creatorcontrib><title>Enrichment of high-quality spermatozoa in bovine semen: relative effectiveness of three filtration matrixes</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>To be practical, any method for improving bull semen must yield a large quantity of motile spermatozoa. Some separation methods based on physical properties, e.g. filtration, chromatography, centrifugation, washing and pooling, have been reported as satisfactory, but generally are not repeatable. Nevertheless, filtration methods appear to allow the attainment of an acceptable number of spermatozoa, thus allowing such a technique to be introduced in the production of standard bovine semen doses for artificial insemination. The aim of this work was to evaluate systematically the relative effects of three filtration matrixes (silica oxide, glass beads or Sephadexä™) on the improvement of whole ejaculate quality. Analysis of the type of matrix and the volume and height of the filtration column was performed. The only characteristic of the columns that appears to influence ejaculate quality after filtering is the matrix volume. While all matrixes produced improvement of semen quality, SephadexTM was better than the other matrixes tested. An explanation for the mechanism of column filtration is proposed.</description><subject>Acrosome - physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Bull</subject><subject>Cattle</subject><subject>Cell Adhesion</subject><subject>Cell Separation - methods</subject><subject>Cell Size</subject><subject>Coloring Agents</subject><subject>column filtration</subject><subject>Eosine Yellowish-(YS)</subject><subject>Filtration</subject><subject>Glass</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Hypotonic Solutions</subject><subject>Linear Models</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microspheres</subject><subject>Other methods of contraception. Sterilization</subject><subject>semen quality</subject><subject>Sperm Count</subject><subject>Sperm Motility</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - physiology</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkVtv1DAQhS0EoqvSv4D8gHhLGF_iOIiXqpQtoioSKoI3y3EmrLe5bO1s2eXX47BLYV5mpPnO0diHEMogZyDVm3XOpKgy4CXPOQDLASQT-e4JWTwunpIFCBCZLFR1Qs5iXEMqWZSllM_JCWNCg-DVgtxdDsG7VY_DRMeWrvyPVXa_tZ2f9jRuMPR2Gn-NlvqB1uODH5BGTPBbGrCzk39Aim2Lbp4GjHH2mFYBkba-m0IixoEmj-B3GF-QZ63tIp4d-yn5-uHy9uIqu_68_Hhxfp05KXi6GWtZFxIUaNfUJW-FtrqxqGSjwfGGAbO61igb1FWl0quUU5UD7pgC1VhxSl4ffDdhvN9inEzvo8OuswOO22hK0BUUskrgyyO4rXtszCb43oa9-fs9CXh1BGx0tmuDHZyP_zjQIHmRsHcH7KfvcP_f2syBmbWZczFzLmYOzPwJzOzM-c37NCR5dpD7OOHuUW7DnVGlKAvz7WZpviw_fRe3EsyV-A3-YJjI</recordid><startdate>200105</startdate><enddate>200105</enddate><creator>Cisale, H. O.</creator><creator>Fischman, M. L.</creator><creator>Blasi, C. D.</creator><creator>Fernandez, H. A.</creator><creator>Gledhill, B. L.</creator><general>Blackwell Science, Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200105</creationdate><title>Enrichment of high-quality spermatozoa in bovine semen: relative effectiveness of three filtration matrixes</title><author>Cisale, H. O. ; Fischman, M. L. ; Blasi, C. D. ; Fernandez, H. A. ; Gledhill, B. L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4323-4eb4b540608cdb72f38a8dae64d80c2d101a8b8e4de89960456c69c02c1606da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acrosome - physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Bull</topic><topic>Cattle</topic><topic>Cell Adhesion</topic><topic>Cell Separation - methods</topic><topic>Cell Size</topic><topic>Coloring Agents</topic><topic>column filtration</topic><topic>Eosine Yellowish-(YS)</topic><topic>Filtration</topic><topic>Glass</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Hypotonic Solutions</topic><topic>Linear Models</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microspheres</topic><topic>Other methods of contraception. Sterilization</topic><topic>semen quality</topic><topic>Sperm Count</topic><topic>Sperm Motility</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cisale, H. O.</creatorcontrib><creatorcontrib>Fischman, M. L.</creatorcontrib><creatorcontrib>Blasi, C. D.</creatorcontrib><creatorcontrib>Fernandez, H. A.</creatorcontrib><creatorcontrib>Gledhill, B. L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cisale, H. O.</au><au>Fischman, M. L.</au><au>Blasi, C. D.</au><au>Fernandez, H. A.</au><au>Gledhill, B. L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enrichment of high-quality spermatozoa in bovine semen: relative effectiveness of three filtration matrixes</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2001-05</date><risdate>2001</risdate><volume>33</volume><issue>3</issue><spage>143</spage><epage>150</epage><pages>143-150</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><coden>ANDRDQ</coden><abstract>To be practical, any method for improving bull semen must yield a large quantity of motile spermatozoa. Some separation methods based on physical properties, e.g. filtration, chromatography, centrifugation, washing and pooling, have been reported as satisfactory, but generally are not repeatable. Nevertheless, filtration methods appear to allow the attainment of an acceptable number of spermatozoa, thus allowing such a technique to be introduced in the production of standard bovine semen doses for artificial insemination. The aim of this work was to evaluate systematically the relative effects of three filtration matrixes (silica oxide, glass beads or Sephadexä™) on the improvement of whole ejaculate quality. Analysis of the type of matrix and the volume and height of the filtration column was performed. The only characteristic of the columns that appears to influence ejaculate quality after filtering is the matrix volume. While all matrixes produced improvement of semen quality, SephadexTM was better than the other matrixes tested. An explanation for the mechanism of column filtration is proposed.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science, Ltd</pub><pmid>11380329</pmid><doi>10.1046/j.1439-0272.2001.00413.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0303-4569 |
| ispartof | Andrologia, 2001-05, Vol.33 (3), p.143-150 |
| issn | 0303-4569 1439-0272 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70890549 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Acrosome - physiology Animals Biological and medical sciences Birth control Bull Cattle Cell Adhesion Cell Separation - methods Cell Size Coloring Agents column filtration Eosine Yellowish-(YS) Filtration Glass Gynecology. Andrology. Obstetrics Hypotonic Solutions Linear Models Male Medical sciences Microspheres Other methods of contraception. Sterilization semen quality Sperm Count Sperm Motility Spermatozoa - cytology Spermatozoa - physiology |
| title | Enrichment of high-quality spermatozoa in bovine semen: relative effectiveness of three filtration matrixes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T16%3A27%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enrichment%20of%20high-quality%20spermatozoa%20in%20bovine%20semen:%20relative%20effectiveness%20of%20three%20filtration%20matrixes&rft.jtitle=Andrologia&rft.au=Cisale,%20H.%20O.&rft.date=2001-05&rft.volume=33&rft.issue=3&rft.spage=143&rft.epage=150&rft.pages=143-150&rft.issn=0303-4569&rft.eissn=1439-0272&rft.coden=ANDRDQ&rft_id=info:doi/10.1046/j.1439-0272.2001.00413.x&rft_dat=%3Cproquest_pubme%3E70890549%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70890549&rft_id=info:pmid/11380329&rfr_iscdi=true |