High-Throughput Flow Cytometric DNA Fragment Sizing

The rate of detection and sizing of individual fluorescently labeled DNA fragments in conventional single-molecule flow cytometry (SMFC) is limited by optical saturation, photon-counting statistics, and fragment overlap to ∼100 fragments/s. We have increased the detection rate for DNA fragment sizin...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical chemistry (Washington) 2000-01, Vol.72 (1), p.37-41
Hauptverfasser:	Van Orden, Alan, Keller, Richard A, Ambrose, W. Patrick
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda Bacteriophage M13 Biochemistry Biological and medical sciences Deoxyribonucleic acid Diverse techniques DNA DNA, Viral - analysis DNA, Viral - chemistry Flow Cytometry - methods Fluorescence Fundamental and applied biological sciences. Psychology Methods Molecular and cellular biology Nucleic Acid Conformation Scientific imaging single-molecule flow cytometry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!