Physiological Evidence for an Interaction between Helix XI and Helices I, II, and V in the Melibiose Carrier of Escherichia coli
In a previous study 23 residues in helix XI of the cysteine-less melibiose carrier were changed individually to cysteine. Several of these cysteine mutants (K377C, A383C, F385C, L391C, G395C) had low transport activity and they were white on melibiose MacConkey fermentation plates. After several day...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-02, Vol.268 (2), p.409-413 |
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| creator | Ding, Ping Z. Wilson, T.Hastings |
| description | In a previous study 23 residues in helix XI of the cysteine-less melibiose carrier were changed individually to cysteine. Several of these cysteine mutants (K377C, A383C, F385C, L391C, G395C) had low transport activity and they were white on melibiose MacConkey fermentation plates. After several days of incubation of these white clones on melibiose MacConkey plates a rare red mutant appeared. The plasmid DNA was then isolated and sequenced. The two second site revertants from K377C were I22S and D59A. This change of aspartic acid to a neutral residue suggests that physiologically there is an interaction between K377 and D59 (possibly a salt bridge). The revertants from A383C were in positions 20 (F20L) and 22 (I22S and I22N). Revertants of F385C were intrahelical changes (I387M and A388G) and a change in C-terminal loop (R441C). Revertants of L391C were in helix I (I22N, I22T and D19E) and helix V (A152S). Revertants of G395C were in helix I (D19E and I22N). We suggest that there is an interaction between helix XI and helices I, II, and V and proximity between these helices. |
| doi_str_mv | 10.1006/bbrc.2000.2149 |
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Several of these cysteine mutants (K377C, A383C, F385C, L391C, G395C) had low transport activity and they were white on melibiose MacConkey fermentation plates. After several days of incubation of these white clones on melibiose MacConkey plates a rare red mutant appeared. The plasmid DNA was then isolated and sequenced. The two second site revertants from K377C were I22S and D59A. This change of aspartic acid to a neutral residue suggests that physiologically there is an interaction between K377 and D59 (possibly a salt bridge). The revertants from A383C were in positions 20 (F20L) and 22 (I22S and I22N). Revertants of F385C were intrahelical changes (I387M and A388G) and a change in C-terminal loop (R441C). Revertants of L391C were in helix I (I22N, I22T and D19E) and helix V (A152S). Revertants of G395C were in helix I (D19E and I22N). We suggest that there is an interaction between helix XI and helices I, II, and V and proximity between these helices.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2000.2149</identifier><identifier>PMID: 10679218</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>4-Chloromercuribenzenesulfonate - pharmacology ; Biological Transport - drug effects ; Escherichia coli ; Escherichia coli - chemistry ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Kinetics ; Lithium - pharmacology ; melibiose ; Melibiose - metabolism ; Membrane Transport Proteins - chemistry ; Membrane Transport Proteins - drug effects ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Mutation ; Protein Structure, Secondary ; Sodium - pharmacology ; Symporters</subject><ispartof>Biochemical and biophysical research communications, 2000-02, Vol.268 (2), p.409-413</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-64432cf574e288f5a78db6f8d4472badffa9d79644a1400e6c01f2f088fca3e13</citedby><cites>FETCH-LOGICAL-c371t-64432cf574e288f5a78db6f8d4472badffa9d79644a1400e6c01f2f088fca3e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.2000.2149$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10679218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ding, Ping Z.</creatorcontrib><creatorcontrib>Wilson, T.Hastings</creatorcontrib><title>Physiological Evidence for an Interaction between Helix XI and Helices I, II, and V in the Melibiose Carrier of Escherichia coli</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>In a previous study 23 residues in helix XI of the cysteine-less melibiose carrier were changed individually to cysteine. Several of these cysteine mutants (K377C, A383C, F385C, L391C, G395C) had low transport activity and they were white on melibiose MacConkey fermentation plates. After several days of incubation of these white clones on melibiose MacConkey plates a rare red mutant appeared. The plasmid DNA was then isolated and sequenced. The two second site revertants from K377C were I22S and D59A. This change of aspartic acid to a neutral residue suggests that physiologically there is an interaction between K377 and D59 (possibly a salt bridge). The revertants from A383C were in positions 20 (F20L) and 22 (I22S and I22N). Revertants of F385C were intrahelical changes (I387M and A388G) and a change in C-terminal loop (R441C). Revertants of L391C were in helix I (I22N, I22T and D19E) and helix V (A152S). Revertants of G395C were in helix I (D19E and I22N). We suggest that there is an interaction between helix XI and helices I, II, and V and proximity between these helices.</description><subject>4-Chloromercuribenzenesulfonate - pharmacology</subject><subject>Biological Transport - drug effects</subject><subject>Escherichia coli</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Kinetics</subject><subject>Lithium - pharmacology</subject><subject>melibiose</subject><subject>Melibiose - metabolism</subject><subject>Membrane Transport Proteins - chemistry</subject><subject>Membrane Transport Proteins - drug effects</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Mutation</subject><subject>Protein Structure, Secondary</subject><subject>Sodium - pharmacology</subject><subject>Symporters</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTFvFDEQRi0EIpdAS4lcUbHHjNe33i3R6SArBUEBKJ3ltcec0d462HuBdPz0eLkUNIjCGnnmzVfMY-wFwhoBmjfDkOxaAMBaoOwesRVCB5VAkI_ZqrSbSnR4fcbOc_4OgCib7ik7Q2hUJ7Bdsd-f9nc5xDF-C9aMfHcbHE2WuI-Jm4n300zJ2DnEiQ80_ySa-CWN4Re_7svc_flYyrx_zfvyltZXHiY-74l_KLMhxEx8a1IKlHj0fJftnlKw-2C4jWN4xp54M2Z6_lAv2Jd3u8_by-rq4_t--_aqsrXCuWqkrIX1GyVJtK3fGNW6ofGtk1KJwTjvTedUVzCDEoAaC-iFh8JaUxPWF-zVKfcmxR9HyrM-hGxpHM1E8Zi1KqgStfoviC20KDeigOsTaFPMOZHXNykcTLrTCHqRoxc5epGjFzll4eVD8nE4kPsLP9koQHsCqBzithxMZxsWHS4ksrN2Mfwr-x7NXZ06</recordid><startdate>20000216</startdate><enddate>20000216</enddate><creator>Ding, Ping Z.</creator><creator>Wilson, T.Hastings</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20000216</creationdate><title>Physiological Evidence for an Interaction between Helix XI and Helices I, II, and V in the Melibiose Carrier of Escherichia coli</title><author>Ding, Ping Z. ; Wilson, T.Hastings</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-64432cf574e288f5a78db6f8d4472badffa9d79644a1400e6c01f2f088fca3e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>4-Chloromercuribenzenesulfonate - pharmacology</topic><topic>Biological Transport - drug effects</topic><topic>Escherichia coli</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Kinetics</topic><topic>Lithium - pharmacology</topic><topic>melibiose</topic><topic>Melibiose - metabolism</topic><topic>Membrane Transport Proteins - chemistry</topic><topic>Membrane Transport Proteins - drug effects</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Mutation</topic><topic>Protein Structure, Secondary</topic><topic>Sodium - pharmacology</topic><topic>Symporters</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Ping Z.</creatorcontrib><creatorcontrib>Wilson, T.Hastings</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Ping Z.</au><au>Wilson, T.Hastings</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiological Evidence for an Interaction between Helix XI and Helices I, II, and V in the Melibiose Carrier of Escherichia coli</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2000-02-16</date><risdate>2000</risdate><volume>268</volume><issue>2</issue><spage>409</spage><epage>413</epage><pages>409-413</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>In a previous study 23 residues in helix XI of the cysteine-less melibiose carrier were changed individually to cysteine. Several of these cysteine mutants (K377C, A383C, F385C, L391C, G395C) had low transport activity and they were white on melibiose MacConkey fermentation plates. After several days of incubation of these white clones on melibiose MacConkey plates a rare red mutant appeared. The plasmid DNA was then isolated and sequenced. The two second site revertants from K377C were I22S and D59A. This change of aspartic acid to a neutral residue suggests that physiologically there is an interaction between K377 and D59 (possibly a salt bridge). The revertants from A383C were in positions 20 (F20L) and 22 (I22S and I22N). Revertants of F385C were intrahelical changes (I387M and A388G) and a change in C-terminal loop (R441C). Revertants of L391C were in helix I (I22N, I22T and D19E) and helix V (A152S). Revertants of G395C were in helix I (D19E and I22N). We suggest that there is an interaction between helix XI and helices I, II, and V and proximity between these helices.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10679218</pmid><doi>10.1006/bbrc.2000.2149</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 4-Chloromercuribenzenesulfonate - pharmacology Biological Transport - drug effects Escherichia coli Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - metabolism Kinetics Lithium - pharmacology melibiose Melibiose - metabolism Membrane Transport Proteins - chemistry Membrane Transport Proteins - drug effects Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Mutation Protein Structure, Secondary Sodium - pharmacology Symporters |
| title | Physiological Evidence for an Interaction between Helix XI and Helices I, II, and V in the Melibiose Carrier of Escherichia coli |
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