Immunolocalization of tumor necrosis factor-α expressing cells in recurrent aphthous ulcer lesions (RAU)
: Tumor necrosis factor (TNF)‐α is a pro‐inflammatory cytokine and crucial mediator in many aspects of immunity. Although several studies have shown that recurrent aphthous ulcers (RAU) can be prevented by treatment that prevents the synthesis of endogenous TNF‐α, little is known about the location...
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| Veröffentlicht in: | Journal of oral pathology & medicine 2000-01, Vol.29 (1), p.19-25 |
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| description | : Tumor necrosis factor (TNF)‐α is a pro‐inflammatory cytokine and crucial mediator in many aspects of immunity. Although several studies have shown that recurrent aphthous ulcers (RAU) can be prevented by treatment that prevents the synthesis of endogenous TNF‐α, little is known about the location and distribution of TNF‐α‐expressing cells at disease sites. The aim of the present work is, therefore, to investigate TNF‐α and its cellular distribution in RAU lesions compared with those in induced oral traumatic ulcers (TUs). Twelve biopsies of RAU lesions of oral mucosa were obtained from 12 patients with RAU. They were compared to a control group consisting of ten samples of induced TUs. All samples were analyzed for TNF‐α expression by using monoclonal mouse anti‐human TNF‐α antibody in avidin‐biotin‐peroxidase complex (ABC) staining. Results were quantified by a semi‐automatic VIDAS image analysis system. TNF‐α immunoreactivity was contained mainly in monocyte/macrophages and lymphocytes within the mononuclear inflammatory infiltrates. TNF‐α was often seen in mast cells and vascular endothelial cells in connective tissue lateral to the inflammatory infiltrates. Interestingly, 32%–60% of the mononuclear cells were found to be TNF‐α immunoreactive in RAU lesions. TNF‐α‐containing cells were more numerous in aphthae (188±46 cells/0.2 mm2) compared with controls (52±14 cells/0.2 mm2, P |
| doi_str_mv | 10.1034/j.1600-0714.2000.290104.x |
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Although several studies have shown that recurrent aphthous ulcers (RAU) can be prevented by treatment that prevents the synthesis of endogenous TNF‐α, little is known about the location and distribution of TNF‐α‐expressing cells at disease sites. The aim of the present work is, therefore, to investigate TNF‐α and its cellular distribution in RAU lesions compared with those in induced oral traumatic ulcers (TUs). Twelve biopsies of RAU lesions of oral mucosa were obtained from 12 patients with RAU. They were compared to a control group consisting of ten samples of induced TUs. All samples were analyzed for TNF‐α expression by using monoclonal mouse anti‐human TNF‐α antibody in avidin‐biotin‐peroxidase complex (ABC) staining. Results were quantified by a semi‐automatic VIDAS image analysis system. TNF‐α immunoreactivity was contained mainly in monocyte/macrophages and lymphocytes within the mononuclear inflammatory infiltrates. TNF‐α was often seen in mast cells and vascular endothelial cells in connective tissue lateral to the inflammatory infiltrates. Interestingly, 32%–60% of the mononuclear cells were found to be TNF‐α immunoreactive in RAU lesions. TNF‐α‐containing cells were more numerous in aphthae (188±46 cells/0.2 mm2) compared with controls (52±14 cells/0.2 mm2, P<0.001). These findings suggest that RAU lesions are characterized by high expression of TNF‐α. Because such expression occurred in the mononuclear inflammatory cells, mast cells and vascular endothelial cells, TNF‐α, which is a major inflammatory mediator, may contribute to the activation and recruitment of leukocytes that are found in RAU lesions.</description><identifier>ISSN: 0904-2512</identifier><identifier>EISSN: 1600-0714</identifier><identifier>DOI: 10.1034/j.1600-0714.2000.290104.x</identifier><identifier>PMID: 10678712</identifier><language>eng</language><publisher>Copenhagen: Munksgaard International Publishers</publisher><subject>Adult ; Biological and medical sciences ; Biomarkers - analysis ; Biopsy ; Dentistry ; Ent. Stomatology ; Female ; Humans ; Immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Middle Aged ; Mouth Mucosa - metabolism ; Mouth Mucosa - pathology ; Non tumoral diseases ; Oral Ulcer - metabolism ; Oral Ulcer - pathology ; Otorhinolaryngology. Stomatology ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Recurrence ; recurrent aphthous ulcers ; Statistics, Nonparametric ; Stomatitis, Aphthous - metabolism ; Stomatitis, Aphthous - pathology ; Tumor Necrosis Factor-alpha - analysis ; Tumor Necrosis Factor-alpha - metabolism ; tumor necrosis factor-α ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>Journal of oral pathology & medicine, 2000-01, Vol.29 (1), p.19-25</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4374-4c0580a5cfa9d075c222dddcb3b4c3afa3c4a3026c7a4fff2de0f28008800573</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1034%2Fj.1600-0714.2000.290104.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1034%2Fj.1600-0714.2000.290104.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4009,27902,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1226761$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10678712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Natah, Sirajedin S.</creatorcontrib><creatorcontrib>Häyrinen-Immonen, Ritva</creatorcontrib><creatorcontrib>Hietanen, Jarkko</creatorcontrib><creatorcontrib>Malmström, Maria</creatorcontrib><creatorcontrib>Konttinen, Yrjö T.</creatorcontrib><title>Immunolocalization of tumor necrosis factor-α expressing cells in recurrent aphthous ulcer lesions (RAU)</title><title>Journal of oral pathology & medicine</title><addtitle>J Oral Pathol Med</addtitle><description>: Tumor necrosis factor (TNF)‐α is a pro‐inflammatory cytokine and crucial mediator in many aspects of immunity. Although several studies have shown that recurrent aphthous ulcers (RAU) can be prevented by treatment that prevents the synthesis of endogenous TNF‐α, little is known about the location and distribution of TNF‐α‐expressing cells at disease sites. The aim of the present work is, therefore, to investigate TNF‐α and its cellular distribution in RAU lesions compared with those in induced oral traumatic ulcers (TUs). Twelve biopsies of RAU lesions of oral mucosa were obtained from 12 patients with RAU. They were compared to a control group consisting of ten samples of induced TUs. All samples were analyzed for TNF‐α expression by using monoclonal mouse anti‐human TNF‐α antibody in avidin‐biotin‐peroxidase complex (ABC) staining. Results were quantified by a semi‐automatic VIDAS image analysis system. TNF‐α immunoreactivity was contained mainly in monocyte/macrophages and lymphocytes within the mononuclear inflammatory infiltrates. TNF‐α was often seen in mast cells and vascular endothelial cells in connective tissue lateral to the inflammatory infiltrates. Interestingly, 32%–60% of the mononuclear cells were found to be TNF‐α immunoreactive in RAU lesions. TNF‐α‐containing cells were more numerous in aphthae (188±46 cells/0.2 mm2) compared with controls (52±14 cells/0.2 mm2, P<0.001). These findings suggest that RAU lesions are characterized by high expression of TNF‐α. Because such expression occurred in the mononuclear inflammatory cells, mast cells and vascular endothelial cells, TNF‐α, which is a major inflammatory mediator, may contribute to the activation and recruitment of leukocytes that are found in RAU lesions.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Biopsy</subject><subject>Dentistry</subject><subject>Ent. Stomatology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mouth Mucosa - metabolism</subject><subject>Mouth Mucosa - pathology</subject><subject>Non tumoral diseases</subject><subject>Oral Ulcer - metabolism</subject><subject>Oral Ulcer - pathology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Recurrence</subject><subject>recurrent aphthous ulcers</subject><subject>Statistics, Nonparametric</subject><subject>Stomatitis, Aphthous - metabolism</subject><subject>Stomatitis, Aphthous - pathology</subject><subject>Tumor Necrosis Factor-alpha - analysis</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>tumor necrosis factor-α</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>0904-2512</issn><issn>1600-0714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAURi0EotPCKyAjIVQWCdc_iZMd1RSGVhUFVNSl5XFs6iGJp3YiprwVL8Iz4SijliULy5Z87ufPB6GXBHICjL_d5KQEyEAQnlMAyGkNBHi-e4QW9zeP0QJq4BktCD1AhzFuAIhgnDxFBwRKUQlCF8iddd3Y-9Zr1bpfanC-x97iYex8wL3RwUcXsVV68CH78xub3TaYGF3_HWvTthG7HgejxxBMP2C1vRlu_Bjx2GoTcGtiyov4-OvJtzfP0BOr2mie7_cjdPXh_dXyY3ZxuTpbnlxkmjPBM66hqEAV2qq6AVFoSmnTNHrN1lwzZRXTXDGgpRaKW2tpY8DSCqBKqxDsCL2eY7fB344mDrJzcaqqepOaSZHAsuYTWM_g9McYjJXb4DoV7iQBOWmWGznJlJNMOWmWs2a5S7Mv9o-M6840_0zOXhPwag-omMzaoHrt4gNHaSlKkrB3M_bTtebu_wvI88vP8zlFZHOEi4PZ3Ueo8EOWgolCXn9aydOqvl6Vyy8S2F8P9aoi</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>Natah, Sirajedin S.</creator><creator>Häyrinen-Immonen, Ritva</creator><creator>Hietanen, Jarkko</creator><creator>Malmström, Maria</creator><creator>Konttinen, Yrjö T.</creator><general>Munksgaard International Publishers</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>Immunolocalization of tumor necrosis factor-α expressing cells in recurrent aphthous ulcer lesions (RAU)</title><author>Natah, Sirajedin S. ; Häyrinen-Immonen, Ritva ; Hietanen, Jarkko ; Malmström, Maria ; Konttinen, Yrjö T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4374-4c0580a5cfa9d075c222dddcb3b4c3afa3c4a3026c7a4fff2de0f28008800573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Biopsy</topic><topic>Dentistry</topic><topic>Ent. Stomatology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mouth Mucosa - metabolism</topic><topic>Mouth Mucosa - pathology</topic><topic>Non tumoral diseases</topic><topic>Oral Ulcer - metabolism</topic><topic>Oral Ulcer - pathology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Recurrence</topic><topic>recurrent aphthous ulcers</topic><topic>Statistics, Nonparametric</topic><topic>Stomatitis, Aphthous - metabolism</topic><topic>Stomatitis, Aphthous - pathology</topic><topic>Tumor Necrosis Factor-alpha - analysis</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>tumor necrosis factor-α</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Natah, Sirajedin S.</creatorcontrib><creatorcontrib>Häyrinen-Immonen, Ritva</creatorcontrib><creatorcontrib>Hietanen, Jarkko</creatorcontrib><creatorcontrib>Malmström, Maria</creatorcontrib><creatorcontrib>Konttinen, Yrjö T.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of oral pathology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Natah, Sirajedin S.</au><au>Häyrinen-Immonen, Ritva</au><au>Hietanen, Jarkko</au><au>Malmström, Maria</au><au>Konttinen, Yrjö T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunolocalization of tumor necrosis factor-α expressing cells in recurrent aphthous ulcer lesions (RAU)</atitle><jtitle>Journal of oral pathology & medicine</jtitle><addtitle>J Oral Pathol Med</addtitle><date>2000-01</date><risdate>2000</risdate><volume>29</volume><issue>1</issue><spage>19</spage><epage>25</epage><pages>19-25</pages><issn>0904-2512</issn><eissn>1600-0714</eissn><abstract>: Tumor necrosis factor (TNF)‐α is a pro‐inflammatory cytokine and crucial mediator in many aspects of immunity. Although several studies have shown that recurrent aphthous ulcers (RAU) can be prevented by treatment that prevents the synthesis of endogenous TNF‐α, little is known about the location and distribution of TNF‐α‐expressing cells at disease sites. The aim of the present work is, therefore, to investigate TNF‐α and its cellular distribution in RAU lesions compared with those in induced oral traumatic ulcers (TUs). Twelve biopsies of RAU lesions of oral mucosa were obtained from 12 patients with RAU. They were compared to a control group consisting of ten samples of induced TUs. All samples were analyzed for TNF‐α expression by using monoclonal mouse anti‐human TNF‐α antibody in avidin‐biotin‐peroxidase complex (ABC) staining. Results were quantified by a semi‐automatic VIDAS image analysis system. TNF‐α immunoreactivity was contained mainly in monocyte/macrophages and lymphocytes within the mononuclear inflammatory infiltrates. TNF‐α was often seen in mast cells and vascular endothelial cells in connective tissue lateral to the inflammatory infiltrates. Interestingly, 32%–60% of the mononuclear cells were found to be TNF‐α immunoreactive in RAU lesions. TNF‐α‐containing cells were more numerous in aphthae (188±46 cells/0.2 mm2) compared with controls (52±14 cells/0.2 mm2, P<0.001). These findings suggest that RAU lesions are characterized by high expression of TNF‐α. Because such expression occurred in the mononuclear inflammatory cells, mast cells and vascular endothelial cells, TNF‐α, which is a major inflammatory mediator, may contribute to the activation and recruitment of leukocytes that are found in RAU lesions.</abstract><cop>Copenhagen</cop><pub>Munksgaard International Publishers</pub><pmid>10678712</pmid><doi>10.1034/j.1600-0714.2000.290104.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Biological and medical sciences Biomarkers - analysis Biopsy Dentistry Ent. Stomatology Female Humans Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Middle Aged Mouth Mucosa - metabolism Mouth Mucosa - pathology Non tumoral diseases Oral Ulcer - metabolism Oral Ulcer - pathology Otorhinolaryngology. Stomatology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Recurrence recurrent aphthous ulcers Statistics, Nonparametric Stomatitis, Aphthous - metabolism Stomatitis, Aphthous - pathology Tumor Necrosis Factor-alpha - analysis Tumor Necrosis Factor-alpha - metabolism tumor necrosis factor-α Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Immunolocalization of tumor necrosis factor-α expressing cells in recurrent aphthous ulcer lesions (RAU) |
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