Transmission of ovine herpesvirus 2 among adult sheep

Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) di...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Veterinary microbiology 2000, Vol.71 (1), p.27-35
Hauptverfasser: Li, Hong, Snowder, Gary, O’Toole, Donal, Crawford, Timothy B
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 35
container_issue 1
container_start_page 27
container_title Veterinary microbiology
container_volume 71
creator Li, Hong
Snowder, Gary
O’Toole, Donal
Crawford, Timothy B
description Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) direct inoculation of either viable leukocytes or whole blood from OHV-2 positive sheep, and (2) horizontal transmission through natural contact with OHV-2 positive sheep. Two groups of OHV-2 negative adult sheep were inoculated with material from infected sheep, one with 5 × 10 8 viable peripheral blood leukocytes (PBL), and the other with 100 ml of whole peripheral blood. No PCR signals were detected in any of the three sheep inoculated with the PBL during the 20 weeks following inoculation. In the group of five sheep inoculated with whole blood, two became PCR-positive at 7 and 8 weeks post-inoculation, respectively, and the remaining three sheep maintained their negative status until termination of the experiment at 20 weeks post-inoculation. In two experiments conducted in different flocks, a total of 20 adult sheep were used to examine horizontal transmission by contact; all animals became PCR-positive within 12 months of mixing the uninfected and infected animals. The results of these experiments support two conclusions. First, the susceptibility to OHV-2 is not limited to young lambs; adult sheep remain fully susceptible. Second, the fact that whole blood, but not PBL, from infected sheep was able to transmit the infection to only two of five inoculated sheep suggests that the infection in peripheral blood cells may be largely non-productive.
doi_str_mv 10.1016/S0378-1135(99)00160-1
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70886891</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378113599001601</els_id><sourcerecordid>17482622</sourcerecordid><originalsourceid>FETCH-LOGICAL-c487t-7b82081232a34fad4d184939c12e319ad84d7620ed4185ddf333322a2aaa7c023</originalsourceid><addsrcrecordid>eNqFkE1LxDAQhoMo7rr6E5QeRPRQzSRpm5xEFr9gwYPrOWSTqRvptmvSLvjv7X6g3pzLwPDMzMtDyCnQa6CQ37xSXsgUgGeXSl3RfkRT2CNDkAVPWSbYPhn-IANyFOMHpVSonB6SAdA8zzIOQ5JNg6njwsfomzppyqRZ-RqTOYYlxpUPXUxYYhZN_Z4Y11VtEueIy2NyUJoq4smuj8jbw_10_JROXh6fx3eT1ApZtGkxk4xKYJwZLkrjhAMpFFcWGHJQxknhipxRdAJk5lzJ-2LMMGNMYSnjI3KxvbsMzWeHsdV9UotVZWpsuqgLKmUuFfwLQiEky9n6YrYFbWhiDFjqZfALE740UL0Wqzdi9dqaVkpvxOr1g7Pdg262QPdna2uyB853gInWVGWv1fr4y7GM0Vz22O0Ww17bymPQ0XqsLTof0LbaNf6fJN-shZJ0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17482622</pqid></control><display><type>article</type><title>Transmission of ovine herpesvirus 2 among adult sheep</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Li, Hong ; Snowder, Gary ; O’Toole, Donal ; Crawford, Timothy B</creator><creatorcontrib>Li, Hong ; Snowder, Gary ; O’Toole, Donal ; Crawford, Timothy B</creatorcontrib><description>Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) direct inoculation of either viable leukocytes or whole blood from OHV-2 positive sheep, and (2) horizontal transmission through natural contact with OHV-2 positive sheep. Two groups of OHV-2 negative adult sheep were inoculated with material from infected sheep, one with 5 × 10 8 viable peripheral blood leukocytes (PBL), and the other with 100 ml of whole peripheral blood. No PCR signals were detected in any of the three sheep inoculated with the PBL during the 20 weeks following inoculation. In the group of five sheep inoculated with whole blood, two became PCR-positive at 7 and 8 weeks post-inoculation, respectively, and the remaining three sheep maintained their negative status until termination of the experiment at 20 weeks post-inoculation. In two experiments conducted in different flocks, a total of 20 adult sheep were used to examine horizontal transmission by contact; all animals became PCR-positive within 12 months of mixing the uninfected and infected animals. The results of these experiments support two conclusions. First, the susceptibility to OHV-2 is not limited to young lambs; adult sheep remain fully susceptible. Second, the fact that whole blood, but not PBL, from infected sheep was able to transmit the infection to only two of five inoculated sheep suggests that the infection in peripheral blood cells may be largely non-productive.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(99)00160-1</identifier><identifier>PMID: 10665531</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Disease Transmission, Infectious - veterinary ; DNA, Viral - analysis ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Gammaherpesvirinae - genetics ; Gammaherpesvirinae - isolation &amp; purification ; Herpesviridae Infections - transmission ; Herpesviridae Infections - veterinary ; Herpesvirus ; Microbiology ; Nasal Mucosa - virology ; Ovine herpesvirus 2 ; Polymerase Chain Reaction - veterinary ; Sheep ; Sheep Diseases - transmission ; Sheep-viruses ; Transmission ; Virology ; Virus Shedding</subject><ispartof>Veterinary microbiology, 2000, Vol.71 (1), p.27-35</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-7b82081232a34fad4d184939c12e319ad84d7620ed4185ddf333322a2aaa7c023</citedby><cites>FETCH-LOGICAL-c487t-7b82081232a34fad4d184939c12e319ad84d7620ed4185ddf333322a2aaa7c023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1135(99)00160-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1252068$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10665531$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Hong</creatorcontrib><creatorcontrib>Snowder, Gary</creatorcontrib><creatorcontrib>O’Toole, Donal</creatorcontrib><creatorcontrib>Crawford, Timothy B</creatorcontrib><title>Transmission of ovine herpesvirus 2 among adult sheep</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) direct inoculation of either viable leukocytes or whole blood from OHV-2 positive sheep, and (2) horizontal transmission through natural contact with OHV-2 positive sheep. Two groups of OHV-2 negative adult sheep were inoculated with material from infected sheep, one with 5 × 10 8 viable peripheral blood leukocytes (PBL), and the other with 100 ml of whole peripheral blood. No PCR signals were detected in any of the three sheep inoculated with the PBL during the 20 weeks following inoculation. In the group of five sheep inoculated with whole blood, two became PCR-positive at 7 and 8 weeks post-inoculation, respectively, and the remaining three sheep maintained their negative status until termination of the experiment at 20 weeks post-inoculation. In two experiments conducted in different flocks, a total of 20 adult sheep were used to examine horizontal transmission by contact; all animals became PCR-positive within 12 months of mixing the uninfected and infected animals. The results of these experiments support two conclusions. First, the susceptibility to OHV-2 is not limited to young lambs; adult sheep remain fully susceptible. Second, the fact that whole blood, but not PBL, from infected sheep was able to transmit the infection to only two of five inoculated sheep suggests that the infection in peripheral blood cells may be largely non-productive.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Disease Transmission, Infectious - veterinary</subject><subject>DNA, Viral - analysis</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gammaherpesvirinae - genetics</subject><subject>Gammaherpesvirinae - isolation &amp; purification</subject><subject>Herpesviridae Infections - transmission</subject><subject>Herpesviridae Infections - veterinary</subject><subject>Herpesvirus</subject><subject>Microbiology</subject><subject>Nasal Mucosa - virology</subject><subject>Ovine herpesvirus 2</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Sheep</subject><subject>Sheep Diseases - transmission</subject><subject>Sheep-viruses</subject><subject>Transmission</subject><subject>Virology</subject><subject>Virus Shedding</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7rr6E5QeRPRQzSRpm5xEFr9gwYPrOWSTqRvptmvSLvjv7X6g3pzLwPDMzMtDyCnQa6CQ37xSXsgUgGeXSl3RfkRT2CNDkAVPWSbYPhn-IANyFOMHpVSonB6SAdA8zzIOQ5JNg6njwsfomzppyqRZ-RqTOYYlxpUPXUxYYhZN_Z4Y11VtEueIy2NyUJoq4smuj8jbw_10_JROXh6fx3eT1ApZtGkxk4xKYJwZLkrjhAMpFFcWGHJQxknhipxRdAJk5lzJ-2LMMGNMYSnjI3KxvbsMzWeHsdV9UotVZWpsuqgLKmUuFfwLQiEky9n6YrYFbWhiDFjqZfALE740UL0Wqzdi9dqaVkpvxOr1g7Pdg262QPdna2uyB853gInWVGWv1fr4y7GM0Vz22O0Ww17bymPQ0XqsLTof0LbaNf6fJN-shZJ0</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Li, Hong</creator><creator>Snowder, Gary</creator><creator>O’Toole, Donal</creator><creator>Crawford, Timothy B</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Transmission of ovine herpesvirus 2 among adult sheep</title><author>Li, Hong ; Snowder, Gary ; O’Toole, Donal ; Crawford, Timothy B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-7b82081232a34fad4d184939c12e319ad84d7620ed4185ddf333322a2aaa7c023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Disease Transmission, Infectious - veterinary</topic><topic>DNA, Viral - analysis</topic><topic>Epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gammaherpesvirinae - genetics</topic><topic>Gammaherpesvirinae - isolation &amp; purification</topic><topic>Herpesviridae Infections - transmission</topic><topic>Herpesviridae Infections - veterinary</topic><topic>Herpesvirus</topic><topic>Microbiology</topic><topic>Nasal Mucosa - virology</topic><topic>Ovine herpesvirus 2</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Sheep</topic><topic>Sheep Diseases - transmission</topic><topic>Sheep-viruses</topic><topic>Transmission</topic><topic>Virology</topic><topic>Virus Shedding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Hong</creatorcontrib><creatorcontrib>Snowder, Gary</creatorcontrib><creatorcontrib>O’Toole, Donal</creatorcontrib><creatorcontrib>Crawford, Timothy B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Hong</au><au>Snowder, Gary</au><au>O’Toole, Donal</au><au>Crawford, Timothy B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transmission of ovine herpesvirus 2 among adult sheep</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2000</date><risdate>2000</risdate><volume>71</volume><issue>1</issue><spage>27</spage><epage>35</epage><pages>27-35</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) direct inoculation of either viable leukocytes or whole blood from OHV-2 positive sheep, and (2) horizontal transmission through natural contact with OHV-2 positive sheep. Two groups of OHV-2 negative adult sheep were inoculated with material from infected sheep, one with 5 × 10 8 viable peripheral blood leukocytes (PBL), and the other with 100 ml of whole peripheral blood. No PCR signals were detected in any of the three sheep inoculated with the PBL during the 20 weeks following inoculation. In the group of five sheep inoculated with whole blood, two became PCR-positive at 7 and 8 weeks post-inoculation, respectively, and the remaining three sheep maintained their negative status until termination of the experiment at 20 weeks post-inoculation. In two experiments conducted in different flocks, a total of 20 adult sheep were used to examine horizontal transmission by contact; all animals became PCR-positive within 12 months of mixing the uninfected and infected animals. The results of these experiments support two conclusions. First, the susceptibility to OHV-2 is not limited to young lambs; adult sheep remain fully susceptible. Second, the fact that whole blood, but not PBL, from infected sheep was able to transmit the infection to only two of five inoculated sheep suggests that the infection in peripheral blood cells may be largely non-productive.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10665531</pmid><doi>10.1016/S0378-1135(99)00160-1</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0378-1135
ispartof Veterinary microbiology, 2000, Vol.71 (1), p.27-35
issn 0378-1135
1873-2542
language eng
recordid cdi_proquest_miscellaneous_70886891
source MEDLINE; Elsevier ScienceDirect Journals
subjects Animals
Biological and medical sciences
Disease Transmission, Infectious - veterinary
DNA, Viral - analysis
Epidemiology
Fundamental and applied biological sciences. Psychology
Gammaherpesvirinae - genetics
Gammaherpesvirinae - isolation & purification
Herpesviridae Infections - transmission
Herpesviridae Infections - veterinary
Herpesvirus
Microbiology
Nasal Mucosa - virology
Ovine herpesvirus 2
Polymerase Chain Reaction - veterinary
Sheep
Sheep Diseases - transmission
Sheep-viruses
Transmission
Virology
Virus Shedding
title Transmission of ovine herpesvirus 2 among adult sheep
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T23%3A17%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transmission%20of%20ovine%20herpesvirus%202%20among%20adult%20sheep&rft.jtitle=Veterinary%20microbiology&rft.au=Li,%20Hong&rft.date=2000&rft.volume=71&rft.issue=1&rft.spage=27&rft.epage=35&rft.pages=27-35&rft.issn=0378-1135&rft.eissn=1873-2542&rft.coden=VMICDQ&rft_id=info:doi/10.1016/S0378-1135(99)00160-1&rft_dat=%3Cproquest_cross%3E17482622%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17482622&rft_id=info:pmid/10665531&rft_els_id=S0378113599001601&rfr_iscdi=true