Transmission of ovine herpesvirus 2 among adult sheep
Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) di...
Gespeichert in:
Veröffentlicht in: | Veterinary microbiology 2000, Vol.71 (1), p.27-35 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 35 |
---|---|
container_issue | 1 |
container_start_page | 27 |
container_title | Veterinary microbiology |
container_volume | 71 |
creator | Li, Hong Snowder, Gary O’Toole, Donal Crawford, Timothy B |
description | Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) direct inoculation of either viable leukocytes or whole blood from OHV-2 positive sheep, and (2) horizontal transmission through natural contact with OHV-2 positive sheep. Two groups of OHV-2 negative adult sheep were inoculated with material from infected sheep, one with 5
×
10
8 viable peripheral blood leukocytes (PBL), and the other with 100
ml of whole peripheral blood. No PCR signals were detected in any of the three sheep inoculated with the PBL during the 20 weeks following inoculation. In the group of five sheep inoculated with whole blood, two became PCR-positive at 7 and 8 weeks post-inoculation, respectively, and the remaining three sheep maintained their negative status until termination of the experiment at 20 weeks post-inoculation. In two experiments conducted in different flocks, a total of 20 adult sheep were used to examine horizontal transmission by contact; all animals became PCR-positive within 12 months of mixing the uninfected and infected animals. The results of these experiments support two conclusions. First, the susceptibility to OHV-2 is not limited to young lambs; adult sheep remain fully susceptible. Second, the fact that whole blood, but not PBL, from infected sheep was able to transmit the infection to only two of five inoculated sheep suggests that the infection in peripheral blood cells may be largely non-productive. |
doi_str_mv | 10.1016/S0378-1135(99)00160-1 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70886891</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378113599001601</els_id><sourcerecordid>17482622</sourcerecordid><originalsourceid>FETCH-LOGICAL-c487t-7b82081232a34fad4d184939c12e319ad84d7620ed4185ddf333322a2aaa7c023</originalsourceid><addsrcrecordid>eNqFkE1LxDAQhoMo7rr6E5QeRPRQzSRpm5xEFr9gwYPrOWSTqRvptmvSLvjv7X6g3pzLwPDMzMtDyCnQa6CQ37xSXsgUgGeXSl3RfkRT2CNDkAVPWSbYPhn-IANyFOMHpVSonB6SAdA8zzIOQ5JNg6njwsfomzppyqRZ-RqTOYYlxpUPXUxYYhZN_Z4Y11VtEueIy2NyUJoq4smuj8jbw_10_JROXh6fx3eT1ApZtGkxk4xKYJwZLkrjhAMpFFcWGHJQxknhipxRdAJk5lzJ-2LMMGNMYSnjI3KxvbsMzWeHsdV9UotVZWpsuqgLKmUuFfwLQiEky9n6YrYFbWhiDFjqZfALE740UL0Wqzdi9dqaVkpvxOr1g7Pdg262QPdna2uyB853gInWVGWv1fr4y7GM0Vz22O0Ww17bymPQ0XqsLTof0LbaNf6fJN-shZJ0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17482622</pqid></control><display><type>article</type><title>Transmission of ovine herpesvirus 2 among adult sheep</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Li, Hong ; Snowder, Gary ; O’Toole, Donal ; Crawford, Timothy B</creator><creatorcontrib>Li, Hong ; Snowder, Gary ; O’Toole, Donal ; Crawford, Timothy B</creatorcontrib><description>Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) direct inoculation of either viable leukocytes or whole blood from OHV-2 positive sheep, and (2) horizontal transmission through natural contact with OHV-2 positive sheep. Two groups of OHV-2 negative adult sheep were inoculated with material from infected sheep, one with 5
×
10
8 viable peripheral blood leukocytes (PBL), and the other with 100
ml of whole peripheral blood. No PCR signals were detected in any of the three sheep inoculated with the PBL during the 20 weeks following inoculation. In the group of five sheep inoculated with whole blood, two became PCR-positive at 7 and 8 weeks post-inoculation, respectively, and the remaining three sheep maintained their negative status until termination of the experiment at 20 weeks post-inoculation. In two experiments conducted in different flocks, a total of 20 adult sheep were used to examine horizontal transmission by contact; all animals became PCR-positive within 12 months of mixing the uninfected and infected animals. The results of these experiments support two conclusions. First, the susceptibility to OHV-2 is not limited to young lambs; adult sheep remain fully susceptible. Second, the fact that whole blood, but not PBL, from infected sheep was able to transmit the infection to only two of five inoculated sheep suggests that the infection in peripheral blood cells may be largely non-productive.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(99)00160-1</identifier><identifier>PMID: 10665531</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Disease Transmission, Infectious - veterinary ; DNA, Viral - analysis ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Gammaherpesvirinae - genetics ; Gammaherpesvirinae - isolation & purification ; Herpesviridae Infections - transmission ; Herpesviridae Infections - veterinary ; Herpesvirus ; Microbiology ; Nasal Mucosa - virology ; Ovine herpesvirus 2 ; Polymerase Chain Reaction - veterinary ; Sheep ; Sheep Diseases - transmission ; Sheep-viruses ; Transmission ; Virology ; Virus Shedding</subject><ispartof>Veterinary microbiology, 2000, Vol.71 (1), p.27-35</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-7b82081232a34fad4d184939c12e319ad84d7620ed4185ddf333322a2aaa7c023</citedby><cites>FETCH-LOGICAL-c487t-7b82081232a34fad4d184939c12e319ad84d7620ed4185ddf333322a2aaa7c023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1135(99)00160-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1252068$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10665531$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Hong</creatorcontrib><creatorcontrib>Snowder, Gary</creatorcontrib><creatorcontrib>O’Toole, Donal</creatorcontrib><creatorcontrib>Crawford, Timothy B</creatorcontrib><title>Transmission of ovine herpesvirus 2 among adult sheep</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) direct inoculation of either viable leukocytes or whole blood from OHV-2 positive sheep, and (2) horizontal transmission through natural contact with OHV-2 positive sheep. Two groups of OHV-2 negative adult sheep were inoculated with material from infected sheep, one with 5
×
10
8 viable peripheral blood leukocytes (PBL), and the other with 100
ml of whole peripheral blood. No PCR signals were detected in any of the three sheep inoculated with the PBL during the 20 weeks following inoculation. In the group of five sheep inoculated with whole blood, two became PCR-positive at 7 and 8 weeks post-inoculation, respectively, and the remaining three sheep maintained their negative status until termination of the experiment at 20 weeks post-inoculation. In two experiments conducted in different flocks, a total of 20 adult sheep were used to examine horizontal transmission by contact; all animals became PCR-positive within 12 months of mixing the uninfected and infected animals. The results of these experiments support two conclusions. First, the susceptibility to OHV-2 is not limited to young lambs; adult sheep remain fully susceptible. Second, the fact that whole blood, but not PBL, from infected sheep was able to transmit the infection to only two of five inoculated sheep suggests that the infection in peripheral blood cells may be largely non-productive.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Disease Transmission, Infectious - veterinary</subject><subject>DNA, Viral - analysis</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gammaherpesvirinae - genetics</subject><subject>Gammaherpesvirinae - isolation & purification</subject><subject>Herpesviridae Infections - transmission</subject><subject>Herpesviridae Infections - veterinary</subject><subject>Herpesvirus</subject><subject>Microbiology</subject><subject>Nasal Mucosa - virology</subject><subject>Ovine herpesvirus 2</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Sheep</subject><subject>Sheep Diseases - transmission</subject><subject>Sheep-viruses</subject><subject>Transmission</subject><subject>Virology</subject><subject>Virus Shedding</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7rr6E5QeRPRQzSRpm5xEFr9gwYPrOWSTqRvptmvSLvjv7X6g3pzLwPDMzMtDyCnQa6CQ37xSXsgUgGeXSl3RfkRT2CNDkAVPWSbYPhn-IANyFOMHpVSonB6SAdA8zzIOQ5JNg6njwsfomzppyqRZ-RqTOYYlxpUPXUxYYhZN_Z4Y11VtEueIy2NyUJoq4smuj8jbw_10_JROXh6fx3eT1ApZtGkxk4xKYJwZLkrjhAMpFFcWGHJQxknhipxRdAJk5lzJ-2LMMGNMYSnjI3KxvbsMzWeHsdV9UotVZWpsuqgLKmUuFfwLQiEky9n6YrYFbWhiDFjqZfALE740UL0Wqzdi9dqaVkpvxOr1g7Pdg262QPdna2uyB853gInWVGWv1fr4y7GM0Vz22O0Ww17bymPQ0XqsLTof0LbaNf6fJN-shZJ0</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Li, Hong</creator><creator>Snowder, Gary</creator><creator>O’Toole, Donal</creator><creator>Crawford, Timothy B</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Transmission of ovine herpesvirus 2 among adult sheep</title><author>Li, Hong ; Snowder, Gary ; O’Toole, Donal ; Crawford, Timothy B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-7b82081232a34fad4d184939c12e319ad84d7620ed4185ddf333322a2aaa7c023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Disease Transmission, Infectious - veterinary</topic><topic>DNA, Viral - analysis</topic><topic>Epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gammaherpesvirinae - genetics</topic><topic>Gammaherpesvirinae - isolation & purification</topic><topic>Herpesviridae Infections - transmission</topic><topic>Herpesviridae Infections - veterinary</topic><topic>Herpesvirus</topic><topic>Microbiology</topic><topic>Nasal Mucosa - virology</topic><topic>Ovine herpesvirus 2</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Sheep</topic><topic>Sheep Diseases - transmission</topic><topic>Sheep-viruses</topic><topic>Transmission</topic><topic>Virology</topic><topic>Virus Shedding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Hong</creatorcontrib><creatorcontrib>Snowder, Gary</creatorcontrib><creatorcontrib>O’Toole, Donal</creatorcontrib><creatorcontrib>Crawford, Timothy B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Hong</au><au>Snowder, Gary</au><au>O’Toole, Donal</au><au>Crawford, Timothy B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transmission of ovine herpesvirus 2 among adult sheep</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2000</date><risdate>2000</risdate><volume>71</volume><issue>1</issue><spage>27</spage><epage>35</epage><pages>27-35</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Previous studies from this laboratory have defined the pattern of acquisition of ovine herpesvirus 2 (OHV-2) in lambs under natural flock conditions. This study examined the question of whether OHV-2 could be transmitted between adult sheep. Two potential routes of transmission were examined: (1) direct inoculation of either viable leukocytes or whole blood from OHV-2 positive sheep, and (2) horizontal transmission through natural contact with OHV-2 positive sheep. Two groups of OHV-2 negative adult sheep were inoculated with material from infected sheep, one with 5
×
10
8 viable peripheral blood leukocytes (PBL), and the other with 100
ml of whole peripheral blood. No PCR signals were detected in any of the three sheep inoculated with the PBL during the 20 weeks following inoculation. In the group of five sheep inoculated with whole blood, two became PCR-positive at 7 and 8 weeks post-inoculation, respectively, and the remaining three sheep maintained their negative status until termination of the experiment at 20 weeks post-inoculation. In two experiments conducted in different flocks, a total of 20 adult sheep were used to examine horizontal transmission by contact; all animals became PCR-positive within 12 months of mixing the uninfected and infected animals. The results of these experiments support two conclusions. First, the susceptibility to OHV-2 is not limited to young lambs; adult sheep remain fully susceptible. Second, the fact that whole blood, but not PBL, from infected sheep was able to transmit the infection to only two of five inoculated sheep suggests that the infection in peripheral blood cells may be largely non-productive.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10665531</pmid><doi>10.1016/S0378-1135(99)00160-1</doi><tpages>9</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0378-1135 |
ispartof | Veterinary microbiology, 2000, Vol.71 (1), p.27-35 |
issn | 0378-1135 1873-2542 |
language | eng |
recordid | cdi_proquest_miscellaneous_70886891 |
source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | Animals Biological and medical sciences Disease Transmission, Infectious - veterinary DNA, Viral - analysis Epidemiology Fundamental and applied biological sciences. Psychology Gammaherpesvirinae - genetics Gammaherpesvirinae - isolation & purification Herpesviridae Infections - transmission Herpesviridae Infections - veterinary Herpesvirus Microbiology Nasal Mucosa - virology Ovine herpesvirus 2 Polymerase Chain Reaction - veterinary Sheep Sheep Diseases - transmission Sheep-viruses Transmission Virology Virus Shedding |
title | Transmission of ovine herpesvirus 2 among adult sheep |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T23%3A17%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transmission%20of%20ovine%20herpesvirus%202%20among%20adult%20sheep&rft.jtitle=Veterinary%20microbiology&rft.au=Li,%20Hong&rft.date=2000&rft.volume=71&rft.issue=1&rft.spage=27&rft.epage=35&rft.pages=27-35&rft.issn=0378-1135&rft.eissn=1873-2542&rft.coden=VMICDQ&rft_id=info:doi/10.1016/S0378-1135(99)00160-1&rft_dat=%3Cproquest_cross%3E17482622%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17482622&rft_id=info:pmid/10665531&rft_els_id=S0378113599001601&rfr_iscdi=true |