Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae
The reference strains of the 12 serotypes of Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apx I, Apx II and Apx III. The toxins are important virulence factors. In the present study, ELISAs with purified Apx I, Apx II and Apx III, respectively, as an...
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| Veröffentlicht in: | Veterinary microbiology 2000, Vol.71 (1), p.81-87 |
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| creator | Nielsen, Ragnhild van den Bosch, Johannes F Plambeck, Tamara Sørensen, Vibeke Nielsen, Jens Peter |
| description | The reference strains of the 12 serotypes of
Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apx I, Apx II and Apx III. The toxins are important virulence factors. In the present study, ELISAs with purified Apx I, Apx II and Apx III, respectively, as antigen were evaluated as candidates for serological diagnosis of
Actinobacillus pleuropneumoniae infection in pigs. The pigs were inoculated with biotype 1, serotypes 1–12, and biotype 2, serotype 14, respectively. A strong humoral antibody response was seen to all the three antigens in most pigs irrespective of the serotype used for inoculation. However, titers to the exotoxins secreted by the serotype used for inoculation were generally highest. The results show that toxin proteins of
Actinobacillus pleuropneumoniae are antigenically related and that a correlation between serotype and secretion of exotoxin is not revealed serologically in the ELISA test. |
| doi_str_mv | 10.1016/S0378-1135(99)00157-1 |
| format | Article |
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Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apx I, Apx II and Apx III. The toxins are important virulence factors. In the present study, ELISAs with purified Apx I, Apx II and Apx III, respectively, as antigen were evaluated as candidates for serological diagnosis of
Actinobacillus pleuropneumoniae infection in pigs. The pigs were inoculated with biotype 1, serotypes 1–12, and biotype 2, serotype 14, respectively. A strong humoral antibody response was seen to all the three antigens in most pigs irrespective of the serotype used for inoculation. However, titers to the exotoxins secreted by the serotype used for inoculation were generally highest. The results show that toxin proteins of
Actinobacillus pleuropneumoniae are antigenically related and that a correlation between serotype and secretion of exotoxin is not revealed serologically in the ELISA test.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(99)00157-1</identifier><identifier>PMID: 10665536</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Actinobacillus Infections - immunology ; Actinobacillus Infections - microbiology ; Actinobacillus Infections - veterinary ; Actinobacillus pleuropneumoniae ; Actinobacillus pleuropneumoniae - immunology ; Actinobacillus pleuropneumoniae - pathogenicity ; Animals ; Antibodies, Bacterial - analysis ; Apx toxins ; Bacterial Proteins - analysis ; Bacterial Proteins - immunology ; Bacterial Toxins - analysis ; Bacterial Toxins - immunology ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; Fundamental and applied biological sciences. Psychology ; Hemolysin Proteins ; Immune Sera ; Microbiology ; Pleuropneumonia - immunology ; Pleuropneumonia - microbiology ; Pleuropneumonia - veterinary ; Specific Pathogen-Free Organisms ; Swine ; Swine Diseases - immunology ; Swine Diseases - microbiology ; Toxins ; Virulence</subject><ispartof>Veterinary microbiology, 2000, Vol.71 (1), p.81-87</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-b5403b51a44131ec6d1a78a80d2ef93bf01e8d41be0185231afe1ac258ede7cd3</citedby><cites>FETCH-LOGICAL-c473t-b5403b51a44131ec6d1a78a80d2ef93bf01e8d41be0185231afe1ac258ede7cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1135(99)00157-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,4026,27930,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1252569$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10665536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nielsen, Ragnhild</creatorcontrib><creatorcontrib>van den Bosch, Johannes F</creatorcontrib><creatorcontrib>Plambeck, Tamara</creatorcontrib><creatorcontrib>Sørensen, Vibeke</creatorcontrib><creatorcontrib>Nielsen, Jens Peter</creatorcontrib><title>Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The reference strains of the 12 serotypes of
Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apx I, Apx II and Apx III. The toxins are important virulence factors. In the present study, ELISAs with purified Apx I, Apx II and Apx III, respectively, as antigen were evaluated as candidates for serological diagnosis of
Actinobacillus pleuropneumoniae infection in pigs. The pigs were inoculated with biotype 1, serotypes 1–12, and biotype 2, serotype 14, respectively. A strong humoral antibody response was seen to all the three antigens in most pigs irrespective of the serotype used for inoculation. However, titers to the exotoxins secreted by the serotype used for inoculation were generally highest. The results show that toxin proteins of
Actinobacillus pleuropneumoniae are antigenically related and that a correlation between serotype and secretion of exotoxin is not revealed serologically in the ELISA test.</description><subject>Actinobacillus Infections - immunology</subject><subject>Actinobacillus Infections - microbiology</subject><subject>Actinobacillus Infections - veterinary</subject><subject>Actinobacillus pleuropneumoniae</subject><subject>Actinobacillus pleuropneumoniae - immunology</subject><subject>Actinobacillus pleuropneumoniae - pathogenicity</subject><subject>Animals</subject><subject>Antibodies, Bacterial - analysis</subject><subject>Apx toxins</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacterial Toxins - analysis</subject><subject>Bacterial Toxins - immunology</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemolysin Proteins</subject><subject>Immune Sera</subject><subject>Microbiology</subject><subject>Pleuropneumonia - immunology</subject><subject>Pleuropneumonia - microbiology</subject><subject>Pleuropneumonia - veterinary</subject><subject>Specific Pathogen-Free Organisms</subject><subject>Swine</subject><subject>Swine Diseases - immunology</subject><subject>Swine Diseases - microbiology</subject><subject>Toxins</subject><subject>Virulence</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EotvSRwD5gFB7CHjiOE5O1araQqWVOJSeLceeCENiBzupujwHD0y2WbW99WCNJX__jDUfIe-BfQYG5ZcbxmWVAXBxVtfnjIGQGbwiK6gkz3JR5K_J6hE5Iscp_WKMFXXJ3pIjYGUpBC9X5N_mTneTHl3wNLRUe-q8dRHNSNH_3fWYdc7_Rktd308-pBAb9CPVKekdPdtsr2_W57QNkVoc59Bjm9E1wTpMdAx0_Il0PdzP13vn0_59PZM-NNq4rpsSHTqcYhg8Tn3wTuM78qbVXcLTQz0ht1ebH5ffsu33r9eX621mCsnHrBEF440AXRTAAU1pQctKV8zm2Na8aRlgZQtokEElcg66RdAmFxValMbyE_Jp6TvE8GfCNKreJYNdpz2GKSnJqqpgkr8IgixkOZ8ZFAtoYkgpYquG6HoddwqY2ntTD97UXoqqa_XgTcGc-3AYMDU92mepRdQMfDwAOhndtVF749ITl4tclPWMXSwYzmu7cxhVMg69wcWpssG98JP_CZe3Lw</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Nielsen, Ragnhild</creator><creator>van den Bosch, Johannes F</creator><creator>Plambeck, Tamara</creator><creator>Sørensen, Vibeke</creator><creator>Nielsen, Jens Peter</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae</title><author>Nielsen, Ragnhild ; van den Bosch, Johannes F ; Plambeck, Tamara ; Sørensen, Vibeke ; Nielsen, Jens Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-b5403b51a44131ec6d1a78a80d2ef93bf01e8d41be0185231afe1ac258ede7cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Actinobacillus Infections - immunology</topic><topic>Actinobacillus Infections - microbiology</topic><topic>Actinobacillus Infections - veterinary</topic><topic>Actinobacillus pleuropneumoniae</topic><topic>Actinobacillus pleuropneumoniae - immunology</topic><topic>Actinobacillus pleuropneumoniae - pathogenicity</topic><topic>Animals</topic><topic>Antibodies, Bacterial - analysis</topic><topic>Apx toxins</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacterial Toxins - analysis</topic><topic>Bacterial Toxins - immunology</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemolysin Proteins</topic><topic>Immune Sera</topic><topic>Microbiology</topic><topic>Pleuropneumonia - immunology</topic><topic>Pleuropneumonia - microbiology</topic><topic>Pleuropneumonia - veterinary</topic><topic>Specific Pathogen-Free Organisms</topic><topic>Swine</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - microbiology</topic><topic>Toxins</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nielsen, Ragnhild</creatorcontrib><creatorcontrib>van den Bosch, Johannes F</creatorcontrib><creatorcontrib>Plambeck, Tamara</creatorcontrib><creatorcontrib>Sørensen, Vibeke</creatorcontrib><creatorcontrib>Nielsen, Jens Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nielsen, Ragnhild</au><au>van den Bosch, Johannes F</au><au>Plambeck, Tamara</au><au>Sørensen, Vibeke</au><au>Nielsen, Jens Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2000</date><risdate>2000</risdate><volume>71</volume><issue>1</issue><spage>81</spage><epage>87</epage><pages>81-87</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The reference strains of the 12 serotypes of
Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apx I, Apx II and Apx III. The toxins are important virulence factors. In the present study, ELISAs with purified Apx I, Apx II and Apx III, respectively, as antigen were evaluated as candidates for serological diagnosis of
Actinobacillus pleuropneumoniae infection in pigs. The pigs were inoculated with biotype 1, serotypes 1–12, and biotype 2, serotype 14, respectively. A strong humoral antibody response was seen to all the three antigens in most pigs irrespective of the serotype used for inoculation. However, titers to the exotoxins secreted by the serotype used for inoculation were generally highest. The results show that toxin proteins of
Actinobacillus pleuropneumoniae are antigenically related and that a correlation between serotype and secretion of exotoxin is not revealed serologically in the ELISA test.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10665536</pmid><doi>10.1016/S0378-1135(99)00157-1</doi><tpages>7</tpages></addata></record> |
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| subjects | Actinobacillus Infections - immunology Actinobacillus Infections - microbiology Actinobacillus Infections - veterinary Actinobacillus pleuropneumoniae Actinobacillus pleuropneumoniae - immunology Actinobacillus pleuropneumoniae - pathogenicity Animals Antibodies, Bacterial - analysis Apx toxins Bacterial Proteins - analysis Bacterial Proteins - immunology Bacterial Toxins - analysis Bacterial Toxins - immunology Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology Hemolysin Proteins Immune Sera Microbiology Pleuropneumonia - immunology Pleuropneumonia - microbiology Pleuropneumonia - veterinary Specific Pathogen-Free Organisms Swine Swine Diseases - immunology Swine Diseases - microbiology Toxins Virulence |
| title | Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae |
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