Discrete spacers for photometric characterization of humic acids separated by capillary isotachophoresis

A group of twenty discrete spacers suitable for photometric characterization of humic acids (HAs) after their isotachophoretic (ITP) separation at pH 10 was found. The spacers, inorganic and organic acids and amino acids of suitable acid–base and migration properties exhibiting no light absorption i...

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Veröffentlicht in:Journal of Chromatography A 2001-05, Vol.916 (1), p.191-200
Hauptverfasser: Nagyová, Iveta, Kaniansky, Dušan
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description A group of twenty discrete spacers suitable for photometric characterization of humic acids (HAs) after their isotachophoretic (ITP) separation at pH 10 was found. The spacers, inorganic and organic acids and amino acids of suitable acid–base and migration properties exhibiting no light absorption in the UV region of the light spectrum, made possible to perform this characterization in a sensitive spike mode of the ITP analysis. Using this approach a complex mixture of humic constituents present in a test HA preparation was separated into 22 fractions migrating in the interzonal boundary layers formed by the zones of discrete spacers and 21 fractions mixed with the zones of the spacers. A photometric monitoring of the fractions in the ITP stack at a 405 nm detection wavelength provided an adequate selectivity and sensitivity into the characterization. Relative sizes of the detected fractions of the test HA preparation ranged from 0.2–0.3 to 27.5% (based on the response of the phototometric detector at 405 nm). The fractions representing ca. 0.2–0.3% of the total peak area could be still quantified when 800 ng of the test preparation was loaded onto the ITP column. A typical repeatability of the total area of the detection signal corresponding to humic constituents in the ITP stack was ca. 2.5%. Repeatabilities of the peak areas of the fractions of the humic constituents defined by the spacers ranged from 2 to 6% for the fractions representing 1% or more of the total area and from 8 to 12% for those representing less than 1%. No marks of aggregations of the humic constituents were detected and reproducible ITP profiles (fingerprints) of the studied humic preparation were achieved under the developed working conditions.
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The spacers, inorganic and organic acids and amino acids of suitable acid–base and migration properties exhibiting no light absorption in the UV region of the light spectrum, made possible to perform this characterization in a sensitive spike mode of the ITP analysis. Using this approach a complex mixture of humic constituents present in a test HA preparation was separated into 22 fractions migrating in the interzonal boundary layers formed by the zones of discrete spacers and 21 fractions mixed with the zones of the spacers. A photometric monitoring of the fractions in the ITP stack at a 405 nm detection wavelength provided an adequate selectivity and sensitivity into the characterization. Relative sizes of the detected fractions of the test HA preparation ranged from 0.2–0.3 to 27.5% (based on the response of the phototometric detector at 405 nm). The fractions representing ca. 0.2–0.3% of the total peak area could be still quantified when 800 ng of the test preparation was loaded onto the ITP column. A typical repeatability of the total area of the detection signal corresponding to humic constituents in the ITP stack was ca. 2.5%. Repeatabilities of the peak areas of the fractions of the humic constituents defined by the spacers ranged from 2 to 6% for the fractions representing 1% or more of the total area and from 8 to 12% for those representing less than 1%. No marks of aggregations of the humic constituents were detected and reproducible ITP profiles (fingerprints) of the studied humic preparation were achieved under the developed working conditions.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(00)01095-5</identifier><identifier>PMID: 11382291</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Agronomy. 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The spacers, inorganic and organic acids and amino acids of suitable acid–base and migration properties exhibiting no light absorption in the UV region of the light spectrum, made possible to perform this characterization in a sensitive spike mode of the ITP analysis. Using this approach a complex mixture of humic constituents present in a test HA preparation was separated into 22 fractions migrating in the interzonal boundary layers formed by the zones of discrete spacers and 21 fractions mixed with the zones of the spacers. A photometric monitoring of the fractions in the ITP stack at a 405 nm detection wavelength provided an adequate selectivity and sensitivity into the characterization. Relative sizes of the detected fractions of the test HA preparation ranged from 0.2–0.3 to 27.5% (based on the response of the phototometric detector at 405 nm). 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Psychology</topic><topic>Humic acids</topic><topic>Humic substances</topic><topic>Humic Substances - chemistry</topic><topic>Humic Substances - isolation &amp; purification</topic><topic>Organic matter</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>Reproducibility of Results</topic><topic>Soil science</topic><topic>Spectrophotometry, Ultraviolet - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagyová, Iveta</creatorcontrib><creatorcontrib>Kaniansky, Dušan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagyová, Iveta</au><au>Kaniansky, Dušan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Discrete spacers for photometric characterization of humic acids separated by capillary isotachophoresis</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2001-05-04</date><risdate>2001</risdate><volume>916</volume><issue>1</issue><spage>191</spage><epage>200</epage><pages>191-200</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A group of twenty discrete spacers suitable for photometric characterization of humic acids (HAs) after their isotachophoretic (ITP) separation at pH 10 was found. 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subjects Agronomy. Soil science and plant productions
Biological and medical sciences
Chemical, physicochemical, biochemical and biological properties
Electrophoresis - methods
Fulvic acids
Fundamental and applied biological sciences. Psychology
Humic acids
Humic substances
Humic Substances - chemistry
Humic Substances - isolation & purification
Organic matter
Physics, chemistry, biochemistry and biology of agricultural and forest soils
Reproducibility of Results
Soil science
Spectrophotometry, Ultraviolet - methods
title Discrete spacers for photometric characterization of humic acids separated by capillary isotachophoresis
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