Characterisation of PCV-2 isolates from Spain, Germany and France
The new isolated circovirus variant PCV-2 is discussed to be the etiological agent of a new emerging swine disease with a variable morbidity and high lethality, postweaning multisystemic wasting syndrome (PMWS). PMWS has been diagnosed in North America and West Europe. Clinical signs include dyspnea...
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| Veröffentlicht in: | Virus research 2000, Vol.66 (1), p.65-77 |
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| creator | Mankertz, Annette Domingo, Mariano Folch, Josep M LeCann, Pierre Jestin, André Segalés, Joaquim Chmielewicz, Barbara Plana-Durán, Juan Soike, Dirk |
| description | The new isolated circovirus variant PCV-2 is discussed to be the etiological agent of a new emerging swine disease with a variable morbidity and high lethality, postweaning multisystemic wasting syndrome (PMWS). PMWS has been diagnosed in North America and West Europe. Clinical signs include dyspnea, loss of weight, lymph node enlargement and lymphocyte depletion in lymphoid tissues. This report describes the characterisation of PCV-2 isolates from animals affected with PMWS from Germany, Spain and France. We could demonstrate the presence of circovirus by electron microscope, in situ hybridisation and PCR. PCR revealed incidence of PCV-2 in many tissues of one infected animal with the exception of heart and nervous system. The phylogenetic analysis of all PCV-2 isolates yet published in the database, showed relationship of isolates from Spain, Germany and France, with three sequences from Canada determined recently and two isolates from Taiwan, while other North American sequences display a separate cluster. PCR screening of randomly collected organ samples from German pigs not affected with PMWS, revealed a rate of infection with PCV-1 of 5% and with PCV-2 of 26.8%, while blood samples showed a lower incidence. |
| doi_str_mv | 10.1016/S0168-1702(99)00122-7 |
| format | Article |
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PMWS has been diagnosed in North America and West Europe. Clinical signs include dyspnea, loss of weight, lymph node enlargement and lymphocyte depletion in lymphoid tissues. This report describes the characterisation of PCV-2 isolates from animals affected with PMWS from Germany, Spain and France. We could demonstrate the presence of circovirus by electron microscope, in situ hybridisation and PCR. PCR revealed incidence of PCV-2 in many tissues of one infected animal with the exception of heart and nervous system. The phylogenetic analysis of all PCV-2 isolates yet published in the database, showed relationship of isolates from Spain, Germany and France, with three sequences from Canada determined recently and two isolates from Taiwan, while other North American sequences display a separate cluster. PCR screening of randomly collected organ samples from German pigs not affected with PMWS, revealed a rate of infection with PCV-1 of 5% and with PCV-2 of 26.8%, while blood samples showed a lower incidence.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/S0168-1702(99)00122-7</identifier><identifier>PMID: 10653918</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Animals, Newborn ; Base Sequence ; Circoviridae ; Circoviridae Infections - epidemiology ; Circoviridae Infections - pathology ; Circoviridae Infections - veterinary ; Circoviridae Infections - virology ; Circovirus ; Circovirus - classification ; Circovirus - genetics ; Circovirus - isolation & purification ; DNA, Viral - genetics ; dyspnea ; France ; France - epidemiology ; Genome, Viral ; Genomic sequence ; Germany ; Germany - epidemiology ; In Situ Hybridization ; Microscopy, Electron ; Molecular Sequence Data ; PCV-2 ; Phylogeny ; pigs ; Polymerase Chain Reaction ; Porcine circovirus ; postweaning multisystemic wasting syndrome ; Postweaning multisystemic wasting syndrome (PMWS) ; Sequence Analysis, DNA ; Spain ; Spain - epidemiology ; Swine ; Swine Diseases - epidemiology ; Swine Diseases - pathology ; Swine Diseases - virology ; Wasting Syndrome - epidemiology ; Wasting Syndrome - pathology ; Wasting Syndrome - veterinary ; Wasting Syndrome - virology</subject><ispartof>Virus research, 2000, Vol.66 (1), p.65-77</ispartof><rights>2000 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-52bc77b13a16e92bdf5580ac55606da7ef59787437e945cb34aa824d6da5c9ea3</citedby><cites>FETCH-LOGICAL-c392t-52bc77b13a16e92bdf5580ac55606da7ef59787437e945cb34aa824d6da5c9ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-1702(99)00122-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10653918$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mankertz, Annette</creatorcontrib><creatorcontrib>Domingo, Mariano</creatorcontrib><creatorcontrib>Folch, Josep M</creatorcontrib><creatorcontrib>LeCann, Pierre</creatorcontrib><creatorcontrib>Jestin, André</creatorcontrib><creatorcontrib>Segalés, Joaquim</creatorcontrib><creatorcontrib>Chmielewicz, Barbara</creatorcontrib><creatorcontrib>Plana-Durán, Juan</creatorcontrib><creatorcontrib>Soike, Dirk</creatorcontrib><title>Characterisation of PCV-2 isolates from Spain, Germany and France</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>The new isolated circovirus variant PCV-2 is discussed to be the etiological agent of a new emerging swine disease with a variable morbidity and high lethality, postweaning multisystemic wasting syndrome (PMWS). PMWS has been diagnosed in North America and West Europe. Clinical signs include dyspnea, loss of weight, lymph node enlargement and lymphocyte depletion in lymphoid tissues. This report describes the characterisation of PCV-2 isolates from animals affected with PMWS from Germany, Spain and France. We could demonstrate the presence of circovirus by electron microscope, in situ hybridisation and PCR. PCR revealed incidence of PCV-2 in many tissues of one infected animal with the exception of heart and nervous system. The phylogenetic analysis of all PCV-2 isolates yet published in the database, showed relationship of isolates from Spain, Germany and France, with three sequences from Canada determined recently and two isolates from Taiwan, while other North American sequences display a separate cluster. PCR screening of randomly collected organ samples from German pigs not affected with PMWS, revealed a rate of infection with PCV-1 of 5% and with PCV-2 of 26.8%, while blood samples showed a lower incidence.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Base Sequence</subject><subject>Circoviridae</subject><subject>Circoviridae Infections - epidemiology</subject><subject>Circoviridae Infections - pathology</subject><subject>Circoviridae Infections - veterinary</subject><subject>Circoviridae Infections - virology</subject><subject>Circovirus</subject><subject>Circovirus - classification</subject><subject>Circovirus - genetics</subject><subject>Circovirus - isolation & purification</subject><subject>DNA, Viral - genetics</subject><subject>dyspnea</subject><subject>France</subject><subject>France - epidemiology</subject><subject>Genome, Viral</subject><subject>Genomic sequence</subject><subject>Germany</subject><subject>Germany - epidemiology</subject><subject>In Situ Hybridization</subject><subject>Microscopy, Electron</subject><subject>Molecular Sequence Data</subject><subject>PCV-2</subject><subject>Phylogeny</subject><subject>pigs</subject><subject>Polymerase Chain Reaction</subject><subject>Porcine circovirus</subject><subject>postweaning multisystemic wasting syndrome</subject><subject>Postweaning multisystemic wasting syndrome (PMWS)</subject><subject>Sequence Analysis, DNA</subject><subject>Spain</subject><subject>Spain - epidemiology</subject><subject>Swine</subject><subject>Swine Diseases - epidemiology</subject><subject>Swine Diseases - pathology</subject><subject>Swine Diseases - virology</subject><subject>Wasting Syndrome - epidemiology</subject><subject>Wasting Syndrome - pathology</subject><subject>Wasting Syndrome - veterinary</subject><subject>Wasting Syndrome - virology</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1Lw0AQhhdRbK3-BCUnUTC6H9ls9iQSbBUEharXZbKZ4EqT1N1U6L93tUW8eZkZmGfmhYeQY0YvGWX51TyWImWK8jOtzyllnKdqh4xZoeKQab5Lxr_IiByE8E4pzYXK98mI0VwKzYoxuSnfwIMd0LsAg-u7pG-Sp_I15YkL_QIGDEnj-zaZL8F1F8kMfQvdOoGuTqYeOouHZK-BRcCjbZ-Ql-ntc3mXPjzO7subh9QKzYdU8soqVTEBLEfNq7qRsqBgpcxpXoPCRmpVqEwo1Jm0lcgACp7VcSetRhATcrr5u_T9xwrDYFoXLC4W0GG_CkbRouA8E_-CLNoRLAqYELkBre9D8NiYpXct-LVh1HxLNj-SzbdBo7X5kWxUvDvZBqyqFus_VxurEbjeABh9fDr0JliH0VXtPNrB1L37J-IL9GKKGQ</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Mankertz, Annette</creator><creator>Domingo, Mariano</creator><creator>Folch, Josep M</creator><creator>LeCann, Pierre</creator><creator>Jestin, André</creator><creator>Segalés, Joaquim</creator><creator>Chmielewicz, Barbara</creator><creator>Plana-Durán, Juan</creator><creator>Soike, Dirk</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Characterisation of PCV-2 isolates from Spain, Germany and France</title><author>Mankertz, Annette ; Domingo, Mariano ; Folch, Josep M ; LeCann, Pierre ; Jestin, André ; Segalés, Joaquim ; Chmielewicz, Barbara ; Plana-Durán, Juan ; Soike, Dirk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-52bc77b13a16e92bdf5580ac55606da7ef59787437e945cb34aa824d6da5c9ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Base Sequence</topic><topic>Circoviridae</topic><topic>Circoviridae Infections - epidemiology</topic><topic>Circoviridae Infections - pathology</topic><topic>Circoviridae Infections - veterinary</topic><topic>Circoviridae Infections - virology</topic><topic>Circovirus</topic><topic>Circovirus - classification</topic><topic>Circovirus - genetics</topic><topic>Circovirus - isolation & purification</topic><topic>DNA, Viral - genetics</topic><topic>dyspnea</topic><topic>France</topic><topic>France - epidemiology</topic><topic>Genome, Viral</topic><topic>Genomic sequence</topic><topic>Germany</topic><topic>Germany - epidemiology</topic><topic>In Situ Hybridization</topic><topic>Microscopy, Electron</topic><topic>Molecular Sequence Data</topic><topic>PCV-2</topic><topic>Phylogeny</topic><topic>pigs</topic><topic>Polymerase Chain Reaction</topic><topic>Porcine circovirus</topic><topic>postweaning multisystemic wasting syndrome</topic><topic>Postweaning multisystemic wasting syndrome (PMWS)</topic><topic>Sequence Analysis, DNA</topic><topic>Spain</topic><topic>Spain - epidemiology</topic><topic>Swine</topic><topic>Swine Diseases - epidemiology</topic><topic>Swine Diseases - pathology</topic><topic>Swine Diseases - virology</topic><topic>Wasting Syndrome - epidemiology</topic><topic>Wasting Syndrome - pathology</topic><topic>Wasting Syndrome - veterinary</topic><topic>Wasting Syndrome - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mankertz, Annette</creatorcontrib><creatorcontrib>Domingo, Mariano</creatorcontrib><creatorcontrib>Folch, Josep M</creatorcontrib><creatorcontrib>LeCann, Pierre</creatorcontrib><creatorcontrib>Jestin, André</creatorcontrib><creatorcontrib>Segalés, Joaquim</creatorcontrib><creatorcontrib>Chmielewicz, Barbara</creatorcontrib><creatorcontrib>Plana-Durán, Juan</creatorcontrib><creatorcontrib>Soike, Dirk</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mankertz, Annette</au><au>Domingo, Mariano</au><au>Folch, Josep M</au><au>LeCann, Pierre</au><au>Jestin, André</au><au>Segalés, Joaquim</au><au>Chmielewicz, Barbara</au><au>Plana-Durán, Juan</au><au>Soike, Dirk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of PCV-2 isolates from Spain, Germany and France</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2000</date><risdate>2000</risdate><volume>66</volume><issue>1</issue><spage>65</spage><epage>77</epage><pages>65-77</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>The new isolated circovirus variant PCV-2 is discussed to be the etiological agent of a new emerging swine disease with a variable morbidity and high lethality, postweaning multisystemic wasting syndrome (PMWS). PMWS has been diagnosed in North America and West Europe. Clinical signs include dyspnea, loss of weight, lymph node enlargement and lymphocyte depletion in lymphoid tissues. This report describes the characterisation of PCV-2 isolates from animals affected with PMWS from Germany, Spain and France. We could demonstrate the presence of circovirus by electron microscope, in situ hybridisation and PCR. PCR revealed incidence of PCV-2 in many tissues of one infected animal with the exception of heart and nervous system. The phylogenetic analysis of all PCV-2 isolates yet published in the database, showed relationship of isolates from Spain, Germany and France, with three sequences from Canada determined recently and two isolates from Taiwan, while other North American sequences display a separate cluster. PCR screening of randomly collected organ samples from German pigs not affected with PMWS, revealed a rate of infection with PCV-1 of 5% and with PCV-2 of 26.8%, while blood samples showed a lower incidence.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10653918</pmid><doi>10.1016/S0168-1702(99)00122-7</doi><tpages>13</tpages></addata></record> |
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| ispartof | Virus research, 2000, Vol.66 (1), p.65-77 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Animals, Newborn Base Sequence Circoviridae Circoviridae Infections - epidemiology Circoviridae Infections - pathology Circoviridae Infections - veterinary Circoviridae Infections - virology Circovirus Circovirus - classification Circovirus - genetics Circovirus - isolation & purification DNA, Viral - genetics dyspnea France France - epidemiology Genome, Viral Genomic sequence Germany Germany - epidemiology In Situ Hybridization Microscopy, Electron Molecular Sequence Data PCV-2 Phylogeny pigs Polymerase Chain Reaction Porcine circovirus postweaning multisystemic wasting syndrome Postweaning multisystemic wasting syndrome (PMWS) Sequence Analysis, DNA Spain Spain - epidemiology Swine Swine Diseases - epidemiology Swine Diseases - pathology Swine Diseases - virology Wasting Syndrome - epidemiology Wasting Syndrome - pathology Wasting Syndrome - veterinary Wasting Syndrome - virology |
| title | Characterisation of PCV-2 isolates from Spain, Germany and France |
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