Isolation of Novel Heart-Specific Genes Using the BodyMap Database
Two novel heart-specific genes, C3orf3 (chromosome 3 open reading frame 3) and MMGL (myomegalin-like), were isolated using BodyMap, a gene expression database based on site-directed 3′ expressed sequence tags (3′-ESTs) which were collected from nonbiased cDNA libraries of various tissues. The cDNA o...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2001-05, Vol.74 (1), p.115-120 |
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| creator | Soejima, Hidenobu Kawamoto, Shoko Akai, Jun Miyoshi, Osamu Arai, Yuji Morohka, Toshifumi Matsuo, Shuzo Niikawa, Norio Kimura, Akinori Okubo, Kousaku Mukai, Tsunehiro |
| description | Two novel heart-specific genes, C3orf3 (chromosome 3 open reading frame 3) and MMGL (myomegalin-like), were isolated using BodyMap, a gene expression database based on site-directed 3′ expressed sequence tags (3′-ESTs) which were collected from nonbiased cDNA libraries of various tissues. The cDNA of C3orf3 was 1667 bp and was composed of 12 exons within a 10-kb-long genomic sequence. MMGL consisted of 8 exons within a genomic sequence of over 70 kb, leading to four alternatively spliced transcripts. Both genes were strongly expressed in heart and also in skeletal muscle. C3orf3 and MMGL were mapped to 3p22 and 1q1, respectively. Subcellular localizations of their putative proteins were determined as being in the cytoplasm for C3orf3 and in the cytoplasm and nucleus for MMGL. This study showed that BodyMap is a useful database for the isolation of tissue-specific genes. |
| doi_str_mv | 10.1006/geno.2001.6527 |
| format | Article |
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The cDNA of C3orf3 was 1667 bp and was composed of 12 exons within a 10-kb-long genomic sequence. MMGL consisted of 8 exons within a genomic sequence of over 70 kb, leading to four alternatively spliced transcripts. Both genes were strongly expressed in heart and also in skeletal muscle. C3orf3 and MMGL were mapped to 3p22 and 1q1, respectively. Subcellular localizations of their putative proteins were determined as being in the cytoplasm for C3orf3 and in the cytoplasm and nucleus for MMGL. This study showed that BodyMap is a useful database for the isolation of tissue-specific genes.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.2001.6527</identifier><identifier>PMID: 11374908</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Alternative Splicing ; Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; C3orf3 gene ; chromosome 1 ; chromosome 3 ; Chromosome Mapping ; Chromosomes, Human, Pair 1 - genetics ; Chromosomes, Human, Pair 3 - genetics ; Databases, Factual ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; DNA, Complementary - isolation & purification ; Exons ; Expressed Sequence Tags ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Genes - genetics ; Genes. Genome ; Humans ; Introns ; Membrane Proteins - genetics ; MMGL gene ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Muscle Proteins ; Myocardium - metabolism ; myomegalin ; Nuclear Proteins - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Analysis, DNA ; Tissue Distribution</subject><ispartof>Genomics (San Diego, Calif.), 2001-05, Vol.74 (1), p.115-120</ispartof><rights>2001 Academic Press</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-af49946479f258bae1c78b71176d5ec9defeeb4d75de750b3d64dac99b73f6883</citedby><cites>FETCH-LOGICAL-c495t-af49946479f258bae1c78b71176d5ec9defeeb4d75de750b3d64dac99b73f6883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/geno.2001.6527$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1103509$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11374908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soejima, Hidenobu</creatorcontrib><creatorcontrib>Kawamoto, Shoko</creatorcontrib><creatorcontrib>Akai, Jun</creatorcontrib><creatorcontrib>Miyoshi, Osamu</creatorcontrib><creatorcontrib>Arai, Yuji</creatorcontrib><creatorcontrib>Morohka, Toshifumi</creatorcontrib><creatorcontrib>Matsuo, Shuzo</creatorcontrib><creatorcontrib>Niikawa, Norio</creatorcontrib><creatorcontrib>Kimura, Akinori</creatorcontrib><creatorcontrib>Okubo, Kousaku</creatorcontrib><creatorcontrib>Mukai, Tsunehiro</creatorcontrib><title>Isolation of Novel Heart-Specific Genes Using the BodyMap Database</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Two novel heart-specific genes, C3orf3 (chromosome 3 open reading frame 3) and MMGL (myomegalin-like), were isolated using BodyMap, a gene expression database based on site-directed 3′ expressed sequence tags (3′-ESTs) which were collected from nonbiased cDNA libraries of various tissues. The cDNA of C3orf3 was 1667 bp and was composed of 12 exons within a 10-kb-long genomic sequence. MMGL consisted of 8 exons within a genomic sequence of over 70 kb, leading to four alternatively spliced transcripts. Both genes were strongly expressed in heart and also in skeletal muscle. C3orf3 and MMGL were mapped to 3p22 and 1q1, respectively. Subcellular localizations of their putative proteins were determined as being in the cytoplasm for C3orf3 and in the cytoplasm and nucleus for MMGL. This study showed that BodyMap is a useful database for the isolation of tissue-specific genes.</description><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>C3orf3 gene</subject><subject>chromosome 1</subject><subject>chromosome 3</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 1 - genetics</subject><subject>Chromosomes, Human, Pair 3 - genetics</subject><subject>Databases, Factual</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - isolation & purification</subject><subject>Exons</subject><subject>Expressed Sequence Tags</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Genes - genetics</subject><subject>Genes. Genome</subject><subject>Humans</subject><subject>Introns</subject><subject>Membrane Proteins - genetics</subject><subject>MMGL gene</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Muscle Proteins</subject><subject>Myocardium - metabolism</subject><subject>myomegalin</subject><subject>Nuclear Proteins - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Tissue Distribution</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0btOwzAUgGELgWgprIwoA2JLsRPHl5EWaJG4DMBsOfZxMUrjYqdIvD2pWgkWxOTl85H9H4ROCR4TjNnlAtowLjAmY1YVfA8NCRYyF4yyfTTEQoicV7QcoKOU3jHGshTFIRoQUnIqsRiiyV0Kje58aLPgssfwCU02Bx27_HkFxjtvshm0kLLX5NtF1r1BNgn260Gvsmvd6VonOEYHTjcJTnbnCL3e3rxM5_n90-xuenWfGyqrLteOSkkZ5dIVlag1EMNFzQnhzFZgpAUHUFPLKwu8wnVpGbXaSFnz0jEhyhG62M5dxfCxhtSppU8Gmka3ENZJ8f67pGDkX0i4EIwz3MPxFpoYUorg1Cr6pY5fimC1yas2edUmr9rk7S-c7Sav6yXYH77r2YPzHdDJ6MZF3RqffjlcVv0SRkhsGfS9Pj1ElYyH1oD1EUynbPB_PeEbGBKVCg</recordid><startdate>20010515</startdate><enddate>20010515</enddate><creator>Soejima, Hidenobu</creator><creator>Kawamoto, Shoko</creator><creator>Akai, Jun</creator><creator>Miyoshi, Osamu</creator><creator>Arai, Yuji</creator><creator>Morohka, Toshifumi</creator><creator>Matsuo, Shuzo</creator><creator>Niikawa, Norio</creator><creator>Kimura, Akinori</creator><creator>Okubo, Kousaku</creator><creator>Mukai, Tsunehiro</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010515</creationdate><title>Isolation of Novel Heart-Specific Genes Using the BodyMap Database</title><author>Soejima, Hidenobu ; Kawamoto, Shoko ; Akai, Jun ; Miyoshi, Osamu ; Arai, Yuji ; Morohka, Toshifumi ; Matsuo, Shuzo ; Niikawa, Norio ; Kimura, Akinori ; Okubo, Kousaku ; Mukai, Tsunehiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-af49946479f258bae1c78b71176d5ec9defeeb4d75de750b3d64dac99b73f6883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alternative Splicing</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>C3orf3 gene</topic><topic>chromosome 1</topic><topic>chromosome 3</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 1 - genetics</topic><topic>Chromosomes, Human, Pair 3 - genetics</topic><topic>Databases, Factual</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - isolation & purification</topic><topic>Exons</topic><topic>Expressed Sequence Tags</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Genes - genetics</topic><topic>Genes. Genome</topic><topic>Humans</topic><topic>Introns</topic><topic>Membrane Proteins - genetics</topic><topic>MMGL gene</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Muscle Proteins</topic><topic>Myocardium - metabolism</topic><topic>myomegalin</topic><topic>Nuclear Proteins - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soejima, Hidenobu</creatorcontrib><creatorcontrib>Kawamoto, Shoko</creatorcontrib><creatorcontrib>Akai, Jun</creatorcontrib><creatorcontrib>Miyoshi, Osamu</creatorcontrib><creatorcontrib>Arai, Yuji</creatorcontrib><creatorcontrib>Morohka, Toshifumi</creatorcontrib><creatorcontrib>Matsuo, Shuzo</creatorcontrib><creatorcontrib>Niikawa, Norio</creatorcontrib><creatorcontrib>Kimura, Akinori</creatorcontrib><creatorcontrib>Okubo, Kousaku</creatorcontrib><creatorcontrib>Mukai, Tsunehiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soejima, Hidenobu</au><au>Kawamoto, Shoko</au><au>Akai, Jun</au><au>Miyoshi, Osamu</au><au>Arai, Yuji</au><au>Morohka, Toshifumi</au><au>Matsuo, Shuzo</au><au>Niikawa, Norio</au><au>Kimura, Akinori</au><au>Okubo, Kousaku</au><au>Mukai, Tsunehiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Novel Heart-Specific Genes Using the BodyMap Database</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2001-05-15</date><risdate>2001</risdate><volume>74</volume><issue>1</issue><spage>115</spage><epage>120</epage><pages>115-120</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Two novel heart-specific genes, C3orf3 (chromosome 3 open reading frame 3) and MMGL (myomegalin-like), were isolated using BodyMap, a gene expression database based on site-directed 3′ expressed sequence tags (3′-ESTs) which were collected from nonbiased cDNA libraries of various tissues. The cDNA of C3orf3 was 1667 bp and was composed of 12 exons within a 10-kb-long genomic sequence. MMGL consisted of 8 exons within a genomic sequence of over 70 kb, leading to four alternatively spliced transcripts. Both genes were strongly expressed in heart and also in skeletal muscle. C3orf3 and MMGL were mapped to 3p22 and 1q1, respectively. Subcellular localizations of their putative proteins were determined as being in the cytoplasm for C3orf3 and in the cytoplasm and nucleus for MMGL. This study showed that BodyMap is a useful database for the isolation of tissue-specific genes.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>11374908</pmid><doi>10.1006/geno.2001.6527</doi><tpages>6</tpages></addata></record> |
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| subjects | Alternative Splicing Amino Acid Sequence Base Sequence Biological and medical sciences Blotting, Northern C3orf3 gene chromosome 1 chromosome 3 Chromosome Mapping Chromosomes, Human, Pair 1 - genetics Chromosomes, Human, Pair 3 - genetics Databases, Factual DNA, Complementary - chemistry DNA, Complementary - genetics DNA, Complementary - isolation & purification Exons Expressed Sequence Tags Female Fundamental and applied biological sciences. Psychology Gene Library Genes - genetics Genes. Genome Humans Introns Membrane Proteins - genetics MMGL gene Molecular and cellular biology Molecular genetics Molecular Sequence Data Muscle Proteins Myocardium - metabolism myomegalin Nuclear Proteins - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Analysis, DNA Tissue Distribution |
| title | Isolation of Novel Heart-Specific Genes Using the BodyMap Database |
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