Methods for the determination of binding constants by capillary electrophoresis

Apparent equilibrium constants for molecular association (e.g., association constants, binding constants, dissociation constants, partition coefficients) can be determined with a variety of different capillary electrophoresis (CE) approaches. In many cases, the investigated association behavior is b...

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Veröffentlicht in:	Electrophoresis 2001-05, Vol.22 (7), p.1419-1427
Hauptverfasser:	Rundlett, Kimber L., Armstrong, Daniel W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cyclodextrins Electrophoresis, Capillary - methods Equilibrium constants Humans Micelles Molecular association Protein Binding Proteins - analysis Pseudophase Review
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description	Apparent equilibrium constants for molecular association (e.g., association constants, binding constants, dissociation constants, partition coefficients) can be determined with a variety of different capillary electrophoresis (CE) approaches. In many cases, the investigated association behavior is between a smaller molecule or ion (i.e., the solute, drug, or analyte of interest) and a larger entity (e.g., proteins, micelles, polymers, chiral selectors such as cyclodextrins, etc.). Each experimental approach has advantages and disadvantages. Frequently, it is the nature of the system being evaluated that determines the optimal experimental approach. Six different CE‐based techniques for evaluating binding constants are reviewed. Examples of each method, and recent references on its use are given.
doi_str_mv	10.1002/1522-2683(200105)22:7<1419::AID-ELPS1419>3.0.CO;2-V
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subjects	Animals Cyclodextrins Electrophoresis, Capillary - methods Equilibrium constants Humans Micelles Molecular association Protein Binding Proteins - analysis Pseudophase Review
title	Methods for the determination of binding constants by capillary electrophoresis
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