Development of a Binding Assay for p53/HDM2 by Using Homogeneous Time-Resolved Fluorescence

The p53 tumor suppressor protein is activated and stabilized in response to DNA damage, resulting in cell cycle arrest or apoptosis. HMD2 is a negative regulator of p53. Binding of p53 by HDM2 traffics p53 from the nucleus to the cytoplasm where it is recognized and targeted for ubiquitin-mediated d...

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Veröffentlicht in:	Analytical biochemistry 2000-02, Vol.278 (1), p.29-38
Hauptverfasser:	Kane, Stefanie A., Fleener, Catherine A., Zhang, Yun S., Davis, Lenora J., Musselman, Amy L., Huang, Pearl S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Biotinylation DNA-Binding Proteins - metabolism Enzyme-Linked Immunosorbent Assay Genes, Tumor Suppressor Glutathione Transferase - genetics Glutathione Transferase - immunology HDM2 HTRF Molecular Sequence Data Nuclear Proteins - metabolism Organometallic Compounds p53 Phosphopeptides - metabolism Protein Binding protein-protein interaction Proto-Oncogene Proteins - analysis Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-mdm2 Recombinant Fusion Proteins - metabolism Spectrometry, Fluorescence - methods Streptavidin Tumor Protein p73 Tumor Suppressor Protein p53 - analysis Tumor Suppressor Protein p53 - metabolism Tumor Suppressor Proteins
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