Silica-based bioactive glasses modulate expression of bone morphogenetic protein-2 mRNA in Saos-2 osteoblasts in vitro
A chemical exchange of the silica gel layer forming on the surface of bioactive glasses is thought to be the principal reaction for bone–bioactive glass bonding. The contribution of biological molecules on cell–bioactive glass interaction is largely unknown. To further analyze the mechanisms involve...
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| Veröffentlicht in: | Biomaterials 2001-06, Vol.22 (12), p.1475-1483 |
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| description | A chemical exchange of the silica gel layer forming on the surface of bioactive glasses is thought to be the principal reaction for bone–bioactive glass bonding. The contribution of biological molecules on cell–bioactive glass interaction is largely unknown. To further analyze the mechanisms involved in efficient bone bonding to bioactive glass, Saos-2 osteoblastic cells with proven osteogenic phenotype were cultured for 4, 7 and 14 days on two bioactive glasses with different Si contents. Culture plates and dishes made of bioactive (BAG, 53% SiO
2), biocompatible (BCG, 58% SiO
2) and control (G0) glasses were extensively conditioned with phosphate buffer and DMEM medium before seeding the cells. Northern hybridization was used for analysis of mRNA levels of collagen type I (Col-I), alkaline phosphatase (ALP) and bone morphogenetic protein-2 (BMP-2). A significant increase was observed in Col-I mRNA levels in cells grown on the two bioactive glasses when compared with those grown on controls at 4 and 7 days (
p |
| doi_str_mv | 10.1016/S0142-9612(00)00288-X |
| format | Article |
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2), biocompatible (BCG, 58% SiO
2) and control (G0) glasses were extensively conditioned with phosphate buffer and DMEM medium before seeding the cells. Northern hybridization was used for analysis of mRNA levels of collagen type I (Col-I), alkaline phosphatase (ALP) and bone morphogenetic protein-2 (BMP-2). A significant increase was observed in Col-I mRNA levels in cells grown on the two bioactive glasses when compared with those grown on controls at 4 and 7 days (
p<0.04). The mRNA level for ALP in the cultures of bioactive glasses-made plates and dishes was also increased over control at 7 days (
p<0.02) and remained this way between BAG and G0 at 14 days. Striking differences in BMP-2 mRNA levels existed between BAG and G0 plates and dishes at 7 days (
p<0.05). BMP-2 mRNA level in BAG group was higher than in BCG group at 4, 7 and 14 days, but without statistical significance. Saos-2 osteoblastic cells with strong ALP staining were mostly seen on BAG plates under a light microscope. In confocal microscopy, a bright FITC-stained F-actin ring was present in the cytoplasm of cells grown on BAG dish, demonstrating an active functional status. Stimulation of the expression of BMP-2 and other bone mRNAs by bioactive glasses in osteoblastic cells suggests biological involvement of bone related growth factors, peptides and cytokines in bone – bioactive glass bonding.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/S0142-9612(00)00288-X</identifier><identifier>PMID: 11374446</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Alkaline phosphatase ; Alkaline Phosphatase - genetics ; Bioactive glass ; Biocompatible Materials - pharmacology ; Biological and medical sciences ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins - genetics ; Collagen - genetics ; Collagen type I ; Gene Expression Regulation - drug effects ; Glass ; Humans ; Kinetics ; Medical sciences ; MRNA ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; Osteosarcoma ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; RNA, Messenger - genetics ; Saos-2 cells ; Silicon Dioxide - pharmacology ; Technology. Biomaterials. Equipments. Material. Instrumentation ; Time Factors ; Transcription, Genetic - drug effects ; Transforming Growth Factor beta ; Tumor Cells, Cultured</subject><ispartof>Biomaterials, 2001-06, Vol.22 (12), p.1475-1483</ispartof><rights>2001 Elsevier Science Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-95a9090b323743c7f32a3f31c0e004c7f7460e1d4ad15140fa4d19291979fa0c3</citedby><cites>FETCH-LOGICAL-c451t-95a9090b323743c7f32a3f31c0e004c7f7460e1d4ad15140fa4d19291979fa0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0142-9612(00)00288-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=955730$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11374446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Tiejun</creatorcontrib><creatorcontrib>Aro, Hannu T.</creatorcontrib><creatorcontrib>Ylänen, Heimo</creatorcontrib><creatorcontrib>Vuorio, Eero</creatorcontrib><title>Silica-based bioactive glasses modulate expression of bone morphogenetic protein-2 mRNA in Saos-2 osteoblasts in vitro</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>A chemical exchange of the silica gel layer forming on the surface of bioactive glasses is thought to be the principal reaction for bone–bioactive glass bonding. The contribution of biological molecules on cell–bioactive glass interaction is largely unknown. To further analyze the mechanisms involved in efficient bone bonding to bioactive glass, Saos-2 osteoblastic cells with proven osteogenic phenotype were cultured for 4, 7 and 14 days on two bioactive glasses with different Si contents. Culture plates and dishes made of bioactive (BAG, 53% SiO
2), biocompatible (BCG, 58% SiO
2) and control (G0) glasses were extensively conditioned with phosphate buffer and DMEM medium before seeding the cells. Northern hybridization was used for analysis of mRNA levels of collagen type I (Col-I), alkaline phosphatase (ALP) and bone morphogenetic protein-2 (BMP-2). A significant increase was observed in Col-I mRNA levels in cells grown on the two bioactive glasses when compared with those grown on controls at 4 and 7 days (
p<0.04). The mRNA level for ALP in the cultures of bioactive glasses-made plates and dishes was also increased over control at 7 days (
p<0.02) and remained this way between BAG and G0 at 14 days. Striking differences in BMP-2 mRNA levels existed between BAG and G0 plates and dishes at 7 days (
p<0.05). BMP-2 mRNA level in BAG group was higher than in BCG group at 4, 7 and 14 days, but without statistical significance. Saos-2 osteoblastic cells with strong ALP staining were mostly seen on BAG plates under a light microscope. In confocal microscopy, a bright FITC-stained F-actin ring was present in the cytoplasm of cells grown on BAG dish, demonstrating an active functional status. Stimulation of the expression of BMP-2 and other bone mRNAs by bioactive glasses in osteoblastic cells suggests biological involvement of bone related growth factors, peptides and cytokines in bone – bioactive glass bonding.</description><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - genetics</subject><subject>Bioactive glass</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Bone Morphogenetic Protein 2</subject><subject>Bone Morphogenetic Proteins - genetics</subject><subject>Collagen - genetics</subject><subject>Collagen type I</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glass</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>MRNA</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>Osteosarcoma</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>RNA, Messenger - genetics</subject><subject>Saos-2 cells</subject><subject>Silicon Dioxide - pharmacology</subject><subject>Technology. Biomaterials. Equipments. Material. Instrumentation</subject><subject>Time Factors</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transforming Growth Factor beta</subject><subject>Tumor Cells, Cultured</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo7rj6EZSAIHporaST7s5JlsV_sCg4CnsL6XT1GunuzKYyg357MzvDepxTeKlfVb3kMfZcwFsBonm3BqFkZRohXwO8AZBdV10_YCvRtV2lDeiHbHWPnLEnRL-haFDyMTsTom6VUs2K7dZhCt5VvSMceB-i8znskN9MjgiJz3HYTi4jxz-bhEQhLjyOvI8Lllra_Io3uGAOnm9SzBiWSvL5-9cLHha-dpGKjJQx9mVepv3tLuQUn7JHo5sInx3Pc_bz44cfl5-rq2-fvlxeXFVeaZEro50BA30ti9_at2MtXT3WwgMCqKJb1QCKQblB6PK40alBGGmEac3owNfn7NVhbnF3u0XKdg7kcZrcgnFLtoWuNQLUSVA2ppZKtydB0elWqhoKqA-gT5Eo4Wg3Kcwu_bUC7D5Cexeh3edjAexdhPa69L04Ltj2Mw7_u46ZFeDlEXDk3TQmt_hA95zRxeV-_fsDheV7dwGTJR9w8TiEhD7bIYYTRv4BfS63pA</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Gao, Tiejun</creator><creator>Aro, Hannu T.</creator><creator>Ylänen, Heimo</creator><creator>Vuorio, Eero</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>F28</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Silica-based bioactive glasses modulate expression of bone morphogenetic protein-2 mRNA in Saos-2 osteoblasts in vitro</title><author>Gao, Tiejun ; Aro, Hannu T. ; Ylänen, Heimo ; Vuorio, Eero</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-95a9090b323743c7f32a3f31c0e004c7f7460e1d4ad15140fa4d19291979fa0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - genetics</topic><topic>Bioactive glass</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Bone Morphogenetic Protein 2</topic><topic>Bone Morphogenetic Proteins - genetics</topic><topic>Collagen - genetics</topic><topic>Collagen type I</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glass</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>MRNA</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - metabolism</topic><topic>Osteosarcoma</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>RNA, Messenger - genetics</topic><topic>Saos-2 cells</topic><topic>Silicon Dioxide - pharmacology</topic><topic>Technology. Biomaterials. Equipments. Material. Instrumentation</topic><topic>Time Factors</topic><topic>Transcription, Genetic - drug effects</topic><topic>Transforming Growth Factor beta</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Tiejun</creatorcontrib><creatorcontrib>Aro, Hannu T.</creatorcontrib><creatorcontrib>Ylänen, Heimo</creatorcontrib><creatorcontrib>Vuorio, Eero</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Tiejun</au><au>Aro, Hannu T.</au><au>Ylänen, Heimo</au><au>Vuorio, Eero</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silica-based bioactive glasses modulate expression of bone morphogenetic protein-2 mRNA in Saos-2 osteoblasts in vitro</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>22</volume><issue>12</issue><spage>1475</spage><epage>1483</epage><pages>1475-1483</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>A chemical exchange of the silica gel layer forming on the surface of bioactive glasses is thought to be the principal reaction for bone–bioactive glass bonding. The contribution of biological molecules on cell–bioactive glass interaction is largely unknown. To further analyze the mechanisms involved in efficient bone bonding to bioactive glass, Saos-2 osteoblastic cells with proven osteogenic phenotype were cultured for 4, 7 and 14 days on two bioactive glasses with different Si contents. Culture plates and dishes made of bioactive (BAG, 53% SiO
2), biocompatible (BCG, 58% SiO
2) and control (G0) glasses were extensively conditioned with phosphate buffer and DMEM medium before seeding the cells. Northern hybridization was used for analysis of mRNA levels of collagen type I (Col-I), alkaline phosphatase (ALP) and bone morphogenetic protein-2 (BMP-2). A significant increase was observed in Col-I mRNA levels in cells grown on the two bioactive glasses when compared with those grown on controls at 4 and 7 days (
p<0.04). The mRNA level for ALP in the cultures of bioactive glasses-made plates and dishes was also increased over control at 7 days (
p<0.02) and remained this way between BAG and G0 at 14 days. Striking differences in BMP-2 mRNA levels existed between BAG and G0 plates and dishes at 7 days (
p<0.05). BMP-2 mRNA level in BAG group was higher than in BCG group at 4, 7 and 14 days, but without statistical significance. Saos-2 osteoblastic cells with strong ALP staining were mostly seen on BAG plates under a light microscope. In confocal microscopy, a bright FITC-stained F-actin ring was present in the cytoplasm of cells grown on BAG dish, demonstrating an active functional status. Stimulation of the expression of BMP-2 and other bone mRNAs by bioactive glasses in osteoblastic cells suggests biological involvement of bone related growth factors, peptides and cytokines in bone – bioactive glass bonding.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11374446</pmid><doi>10.1016/S0142-9612(00)00288-X</doi><tpages>9</tpages></addata></record> |
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| subjects | Alkaline phosphatase Alkaline Phosphatase - genetics Bioactive glass Biocompatible Materials - pharmacology Biological and medical sciences Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - genetics Collagen - genetics Collagen type I Gene Expression Regulation - drug effects Glass Humans Kinetics Medical sciences MRNA Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - metabolism Osteosarcoma Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) RNA, Messenger - genetics Saos-2 cells Silicon Dioxide - pharmacology Technology. Biomaterials. Equipments. Material. Instrumentation Time Factors Transcription, Genetic - drug effects Transforming Growth Factor beta Tumor Cells, Cultured |
| title | Silica-based bioactive glasses modulate expression of bone morphogenetic protein-2 mRNA in Saos-2 osteoblasts in vitro |
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