Dexamethasone‐induced healing of chronic leg ulcers in a patient with defective organization of the extracellular matrix of fibronectin
Previous investigations have shown that skin fibroblasts derived from patients affected by Ehlers–Danlos syndrome (EDS) lack an organized extracellular matrix (ECM) of fibronectin (FN). As retarded wound healing is a sign of EDS, we hypothesized that a young healthy man suffering from chronic recalc...
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| Veröffentlicht in: | British journal of dermatology (1951) 2000-01, Vol.142 (1), p.166-170 |
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| container_title | British journal of dermatology (1951) |
| container_volume | 142 |
| creator | De Panfilis, G. Ghidini, A. Graifemberghi, S. Barlati, S. Zoppi, N. Colombi, M. |
| description | Previous investigations have shown that skin fibroblasts derived from patients affected by Ehlers–Danlos syndrome (EDS) lack an organized extracellular matrix (ECM) of fibronectin (FN). As retarded wound healing is a sign of EDS, we hypothesized that a young healthy man suffering from chronic recalcitrant leg ulcers might be affected by a defect of FN–ECM organization similar to that observed in EDS. Immunofluorescence of cultured skin fibroblasts obtained from skin biopsies from the patient and a control demonstrated that the patient’s fibroblasts lacked FN–ECM organization, in contrast with those of the control; this was restored by 10−7 mol/L dexamethasone (DEX) in vitro. DEX treatment of the patient was associated with healing of his leg ulcers. In conclusion, DEX may be effective in reversing impaired wound healing associated with a lack of FN–ECM organization |
| doi_str_mv | 10.1046/j.1365-2133.2000.03262.x |
| format | Article |
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As retarded wound healing is a sign of EDS, we hypothesized that a young healthy man suffering from chronic recalcitrant leg ulcers might be affected by a defect of FN–ECM organization similar to that observed in EDS. Immunofluorescence of cultured skin fibroblasts obtained from skin biopsies from the patient and a control demonstrated that the patient’s fibroblasts lacked FN–ECM organization, in contrast with those of the control; this was restored by 10−7 mol/L dexamethasone (DEX) in vitro. DEX treatment of the patient was associated with healing of his leg ulcers. In conclusion, DEX may be effective in reversing impaired wound healing associated with a lack of FN–ECM organization</description><identifier>ISSN: 0007-0963</identifier><identifier>EISSN: 1365-2133</identifier><identifier>DOI: 10.1046/j.1365-2133.2000.03262.x</identifier><identifier>PMID: 10651716</identifier><identifier>CODEN: BJDEAZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adult ; Anti-Inflammatory Agents - therapeutic use ; Biological and medical sciences ; Dermatology ; Dexamethasone - therapeutic use ; fibronectin ; Fibronectins - metabolism ; Humans ; Leg Ulcer - drug therapy ; Leg Ulcer - metabolism ; Leg Ulcer - pathology ; leg ulcers ; Male ; Medical sciences ; Skin involvement in other diseases. Miscellaneous. 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As retarded wound healing is a sign of EDS, we hypothesized that a young healthy man suffering from chronic recalcitrant leg ulcers might be affected by a defect of FN–ECM organization similar to that observed in EDS. Immunofluorescence of cultured skin fibroblasts obtained from skin biopsies from the patient and a control demonstrated that the patient’s fibroblasts lacked FN–ECM organization, in contrast with those of the control; this was restored by 10−7 mol/L dexamethasone (DEX) in vitro. DEX treatment of the patient was associated with healing of his leg ulcers. In conclusion, DEX may be effective in reversing impaired wound healing associated with a lack of FN–ECM organization</description><subject>Adult</subject><subject>Anti-Inflammatory Agents - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Dermatology</subject><subject>Dexamethasone - therapeutic use</subject><subject>fibronectin</subject><subject>Fibronectins - metabolism</subject><subject>Humans</subject><subject>Leg Ulcer - drug therapy</subject><subject>Leg Ulcer - metabolism</subject><subject>Leg Ulcer - pathology</subject><subject>leg ulcers</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Skin involvement in other diseases. Miscellaneous. General aspects</subject><subject>wound healing</subject><subject>Wound Healing - drug effects</subject><issn>0007-0963</issn><issn>1365-2133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkctu1DAUhi1ERYfCKyAvELsEXxI7s2ABLbeqEpvuLcc5nniUOIPt0JQVW3Y8I0_SmA6UlS393_nl4w8hTElJSSVe70vKRV0wynnJCCEl4UywcnmENv-Cx2izJrIgW8FP0dMY94RQTmryBJ1SImoqqdignxew6BFSr-Pk4fePX853s4EO96AH53d4stj0YfLO4AF2eB4MhIidxxofdHLgE75xqccdWDDJfQM8hZ327vsaTj6Ppx4wLCloA8MwDzrgUafglpxZ167dedA_QydWDxGeH88zdP3h_fX5p-Lqy8fP52-vCsOrmhWmsprVUjAK6wKSVlvJDbfW6JaxxrRdRbZtzRgQQbesbdZdbMUa3cqGi6bjZ-jVfe0hTF9niEmNLuaXaQ_THJUkjZQVpyv44gjO7QidOgQ36nCr_v7dCrw8AjoaPdigvXHxgWNCSiJX7M09duMGuP2vRmWVaq-yMZWNqaxS_VGpFvXu8iLf-B0LepTQ</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>De Panfilis, G.</creator><creator>Ghidini, A.</creator><creator>Graifemberghi, S.</creator><creator>Barlati, S.</creator><creator>Zoppi, N.</creator><creator>Colombi, M.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>Dexamethasone‐induced healing of chronic leg ulcers in a patient with defective organization of the extracellular matrix of fibronectin</title><author>De Panfilis, G. ; Ghidini, A. ; Graifemberghi, S. ; Barlati, S. ; Zoppi, N. ; Colombi, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3452-c4fa257621e716714973c3ffcab228cbd409b522e06192b8cedf428ab78368d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Anti-Inflammatory Agents - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Dermatology</topic><topic>Dexamethasone - therapeutic use</topic><topic>fibronectin</topic><topic>Fibronectins - metabolism</topic><topic>Humans</topic><topic>Leg Ulcer - drug therapy</topic><topic>Leg Ulcer - metabolism</topic><topic>Leg Ulcer - pathology</topic><topic>leg ulcers</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Skin involvement in other diseases. Miscellaneous. General aspects</topic><topic>wound healing</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Panfilis, G.</creatorcontrib><creatorcontrib>Ghidini, A.</creatorcontrib><creatorcontrib>Graifemberghi, S.</creatorcontrib><creatorcontrib>Barlati, S.</creatorcontrib><creatorcontrib>Zoppi, N.</creatorcontrib><creatorcontrib>Colombi, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of dermatology (1951)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Panfilis, G.</au><au>Ghidini, A.</au><au>Graifemberghi, S.</au><au>Barlati, S.</au><au>Zoppi, N.</au><au>Colombi, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dexamethasone‐induced healing of chronic leg ulcers in a patient with defective organization of the extracellular matrix of fibronectin</atitle><jtitle>British journal of dermatology (1951)</jtitle><addtitle>Br J Dermatol</addtitle><date>2000-01</date><risdate>2000</risdate><volume>142</volume><issue>1</issue><spage>166</spage><epage>170</epage><pages>166-170</pages><issn>0007-0963</issn><eissn>1365-2133</eissn><coden>BJDEAZ</coden><abstract>Previous investigations have shown that skin fibroblasts derived from patients affected by Ehlers–Danlos syndrome (EDS) lack an organized extracellular matrix (ECM) of fibronectin (FN). As retarded wound healing is a sign of EDS, we hypothesized that a young healthy man suffering from chronic recalcitrant leg ulcers might be affected by a defect of FN–ECM organization similar to that observed in EDS. Immunofluorescence of cultured skin fibroblasts obtained from skin biopsies from the patient and a control demonstrated that the patient’s fibroblasts lacked FN–ECM organization, in contrast with those of the control; this was restored by 10−7 mol/L dexamethasone (DEX) in vitro. DEX treatment of the patient was associated with healing of his leg ulcers. In conclusion, DEX may be effective in reversing impaired wound healing associated with a lack of FN–ECM organization</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>10651716</pmid><doi>10.1046/j.1365-2133.2000.03262.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-0963 |
| ispartof | British journal of dermatology (1951), 2000-01, Vol.142 (1), p.166-170 |
| issn | 0007-0963 1365-2133 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adult Anti-Inflammatory Agents - therapeutic use Biological and medical sciences Dermatology Dexamethasone - therapeutic use fibronectin Fibronectins - metabolism Humans Leg Ulcer - drug therapy Leg Ulcer - metabolism Leg Ulcer - pathology leg ulcers Male Medical sciences Skin involvement in other diseases. Miscellaneous. General aspects wound healing Wound Healing - drug effects |
| title | Dexamethasone‐induced healing of chronic leg ulcers in a patient with defective organization of the extracellular matrix of fibronectin |
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