Biochemical and Developmental Evidence That Ooplasmic Maturation of Prepubertal Bovine Oocytes Is Compromised
Our previous studies have shown that oocytes collected from prepubertal calves lack developmental competence. The overall objective of this study was to assess causes by comparing biochemical and physiologic changes during in vitro maturation of oocytes collected from ovaries of adult cattle at slau...
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| Veröffentlicht in: | Biology of reproduction 2001-06, Vol.64 (6), p.1761-1768 |
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| container_title | Biology of reproduction |
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| creator | SALAMONE, D. F DAMIANI, P FISSORE, R. A ROBL, J. M DUBY, R. T |
| description | Our previous studies have shown that oocytes collected from prepubertal calves lack developmental competence. The overall
objective of this study was to assess causes by comparing biochemical and physiologic changes during in vitro maturation of
oocytes collected from ovaries of adult cattle at slaughter and from superstimulated calves ( |
| doi_str_mv | 10.1095/biolreprod64.6.1761 |
| format | Article |
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objective of this study was to assess causes by comparing biochemical and physiologic changes during in vitro maturation of
oocytes collected from ovaries of adult cattle at slaughter and from superstimulated calves (<6 mo old) by either laporotomy
or ultrasound-guided follicular aspiration. Activity and/or concentrations of maturation-promoting factor (MPF), mitogen-activated
protein kinase (MAPK), and inositol 1,4,5-trisphosphate receptor (IP 3 R) were determined by measuring phosphorylation of histone H-1 kinase, phosphorylation of myelin basic protein, or Western
blotting, respectively, and were compared between oocytes collected from calves and for those collected from cows. The activities
of MPF and MAPK and the relative amount of IP 3 R were significantly lower in calf oocytes. The physiologic significance of these observations was determined by assessing
the developmental potential of embryos derived by reciprocal transfer of metaphase II (M-II) chromosomes between cow and calf
ooplasts and transfer of adult cumulus cells (G0/G1) into cow and calf ooplasts. Procedural controls consisted of transfer
of M-II between adult oocytes and parthenogenic activation of adult and calf oocytes. Adult parthenogenically activated oocytes
cleaved and developed to blastocysts at a higher rate than did similarly activated calf oocytes (42.1% vs. 3.4%, P < 0.05). Cleavage was also higher in reciprocal M-II transfer embryos containing adult ooplasm (46.2% vs. 12.0%, P < 0.05). Cleavage (66.7% vs. 21.9%, P < 0.05) and development to blastocyst (20.1% vs. 4.8%, P < 0.05) of nuclear transfer embryos reconstructed from adult cumulus cells was higher after transfer to adult ooplasts. Collectively,
these results support the hypothesis that lack of developmental competence of calf oocytes is due to their failure or inability
to complete ooplasmic maturation.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod64.6.1761</identifier><identifier>PMID: 11369606</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Aging ; Animals ; Biological and medical sciences ; Blastocyst - physiology ; Blotting, Western ; Calcium Channels - analysis ; Cattle - growth & development ; Cleavage Stage, Ovum ; Cytoplasm - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Inositol 1,4,5-Trisphosphate Receptors ; Mammalian female genital system ; Maturation-Promoting Factor - analysis ; Mitogen-Activated Protein Kinases - analysis ; Morphology. Physiology ; Myelin Basic Protein - metabolism ; Nuclear Transfer Techniques ; Oocytes - chemistry ; Oocytes - physiology ; Oocytes - ultrastructure ; Phosphorylation ; Protein Kinases - metabolism ; Receptors, Cytoplasmic and Nuclear - analysis ; Sexual Maturation ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2001-06, Vol.64 (6), p.1761-1768</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1087425$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11369606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SALAMONE, D. F</creatorcontrib><creatorcontrib>DAMIANI, P</creatorcontrib><creatorcontrib>FISSORE, R. A</creatorcontrib><creatorcontrib>ROBL, J. M</creatorcontrib><creatorcontrib>DUBY, R. T</creatorcontrib><title>Biochemical and Developmental Evidence That Ooplasmic Maturation of Prepubertal Bovine Oocytes Is Compromised</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Our previous studies have shown that oocytes collected from prepubertal calves lack developmental competence. The overall
objective of this study was to assess causes by comparing biochemical and physiologic changes during in vitro maturation of
oocytes collected from ovaries of adult cattle at slaughter and from superstimulated calves (<6 mo old) by either laporotomy
or ultrasound-guided follicular aspiration. Activity and/or concentrations of maturation-promoting factor (MPF), mitogen-activated
protein kinase (MAPK), and inositol 1,4,5-trisphosphate receptor (IP 3 R) were determined by measuring phosphorylation of histone H-1 kinase, phosphorylation of myelin basic protein, or Western
blotting, respectively, and were compared between oocytes collected from calves and for those collected from cows. The activities
of MPF and MAPK and the relative amount of IP 3 R were significantly lower in calf oocytes. The physiologic significance of these observations was determined by assessing
the developmental potential of embryos derived by reciprocal transfer of metaphase II (M-II) chromosomes between cow and calf
ooplasts and transfer of adult cumulus cells (G0/G1) into cow and calf ooplasts. Procedural controls consisted of transfer
of M-II between adult oocytes and parthenogenic activation of adult and calf oocytes. Adult parthenogenically activated oocytes
cleaved and developed to blastocysts at a higher rate than did similarly activated calf oocytes (42.1% vs. 3.4%, P < 0.05). Cleavage was also higher in reciprocal M-II transfer embryos containing adult ooplasm (46.2% vs. 12.0%, P < 0.05). Cleavage (66.7% vs. 21.9%, P < 0.05) and development to blastocyst (20.1% vs. 4.8%, P < 0.05) of nuclear transfer embryos reconstructed from adult cumulus cells was higher after transfer to adult ooplasts. Collectively,
these results support the hypothesis that lack of developmental competence of calf oocytes is due to their failure or inability
to complete ooplasmic maturation.</description><subject>Aging</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - physiology</subject><subject>Blotting, Western</subject><subject>Calcium Channels - analysis</subject><subject>Cattle - growth & development</subject><subject>Cleavage Stage, Ovum</subject><subject>Cytoplasm - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Inositol 1,4,5-Trisphosphate Receptors</subject><subject>Mammalian female genital system</subject><subject>Maturation-Promoting Factor - analysis</subject><subject>Mitogen-Activated Protein Kinases - analysis</subject><subject>Morphology. Physiology</subject><subject>Myelin Basic Protein - metabolism</subject><subject>Nuclear Transfer Techniques</subject><subject>Oocytes - chemistry</subject><subject>Oocytes - physiology</subject><subject>Oocytes - ultrastructure</subject><subject>Phosphorylation</subject><subject>Protein Kinases - metabolism</subject><subject>Receptors, Cytoplasmic and Nuclear - analysis</subject><subject>Sexual Maturation</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1O6zAQhS0Egt7CEyAhLxC7FP_FTpZQ4F4kECxgHTn2hBg5cYmTVrw9riiX1Uijb845OoPQKSULSsr8snbBD7AagpViIRdUSbqHZjRnZaaYLPbRjBAiM84lP0J_YnwnhArO-CE6opTLUhI5Q921C6aFzhntse4tvoE1-LDqoB_T5nbtLPQG8EurR_wUVl7HxOJHPU6DHl3ocWjwc4ox1TBsL67D2vWQUPM5QsT3ES9Dl0J2LoI9RgeN9hFOdnOOXu9uX5b_soenv_fLq4esZVKNGc0VWJbz2uSGSV4KBYwRWYqmKQqlmlqzotQ1VxRYU5RWCKJYXRQWiBHGlnyOLr51k_HHBHGskr0B73UPYYqVIoWS2y7m6GwHTnUHtloNrtPDZ_VTUALOd4COqaJm0L1x8ZdLQiIl_W_Yurd24waoYqe9T6q82mw2UlSy2j6IfwGC84U9</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>SALAMONE, D. F</creator><creator>DAMIANI, P</creator><creator>FISSORE, R. A</creator><creator>ROBL, J. M</creator><creator>DUBY, R. T</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Biochemical and Developmental Evidence That Ooplasmic Maturation of Prepubertal Bovine Oocytes Is Compromised</title><author>SALAMONE, D. F ; DAMIANI, P ; FISSORE, R. A ; ROBL, J. M ; DUBY, R. T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-157ed253bc5c263947e220694ff8877fba289ab371e2f89d44072b88de0c4cd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - physiology</topic><topic>Blotting, Western</topic><topic>Calcium Channels - analysis</topic><topic>Cattle - growth & development</topic><topic>Cleavage Stage, Ovum</topic><topic>Cytoplasm - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inositol 1,4,5-Trisphosphate Receptors</topic><topic>Mammalian female genital system</topic><topic>Maturation-Promoting Factor - analysis</topic><topic>Mitogen-Activated Protein Kinases - analysis</topic><topic>Morphology. Physiology</topic><topic>Myelin Basic Protein - metabolism</topic><topic>Nuclear Transfer Techniques</topic><topic>Oocytes - chemistry</topic><topic>Oocytes - physiology</topic><topic>Oocytes - ultrastructure</topic><topic>Phosphorylation</topic><topic>Protein Kinases - metabolism</topic><topic>Receptors, Cytoplasmic and Nuclear - analysis</topic><topic>Sexual Maturation</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SALAMONE, D. F</creatorcontrib><creatorcontrib>DAMIANI, P</creatorcontrib><creatorcontrib>FISSORE, R. A</creatorcontrib><creatorcontrib>ROBL, J. M</creatorcontrib><creatorcontrib>DUBY, R. T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SALAMONE, D. F</au><au>DAMIANI, P</au><au>FISSORE, R. A</au><au>ROBL, J. M</au><au>DUBY, R. T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical and Developmental Evidence That Ooplasmic Maturation of Prepubertal Bovine Oocytes Is Compromised</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>64</volume><issue>6</issue><spage>1761</spage><epage>1768</epage><pages>1761-1768</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Our previous studies have shown that oocytes collected from prepubertal calves lack developmental competence. The overall
objective of this study was to assess causes by comparing biochemical and physiologic changes during in vitro maturation of
oocytes collected from ovaries of adult cattle at slaughter and from superstimulated calves (<6 mo old) by either laporotomy
or ultrasound-guided follicular aspiration. Activity and/or concentrations of maturation-promoting factor (MPF), mitogen-activated
protein kinase (MAPK), and inositol 1,4,5-trisphosphate receptor (IP 3 R) were determined by measuring phosphorylation of histone H-1 kinase, phosphorylation of myelin basic protein, or Western
blotting, respectively, and were compared between oocytes collected from calves and for those collected from cows. The activities
of MPF and MAPK and the relative amount of IP 3 R were significantly lower in calf oocytes. The physiologic significance of these observations was determined by assessing
the developmental potential of embryos derived by reciprocal transfer of metaphase II (M-II) chromosomes between cow and calf
ooplasts and transfer of adult cumulus cells (G0/G1) into cow and calf ooplasts. Procedural controls consisted of transfer
of M-II between adult oocytes and parthenogenic activation of adult and calf oocytes. Adult parthenogenically activated oocytes
cleaved and developed to blastocysts at a higher rate than did similarly activated calf oocytes (42.1% vs. 3.4%, P < 0.05). Cleavage was also higher in reciprocal M-II transfer embryos containing adult ooplasm (46.2% vs. 12.0%, P < 0.05). Cleavage (66.7% vs. 21.9%, P < 0.05) and development to blastocyst (20.1% vs. 4.8%, P < 0.05) of nuclear transfer embryos reconstructed from adult cumulus cells was higher after transfer to adult ooplasts. Collectively,
these results support the hypothesis that lack of developmental competence of calf oocytes is due to their failure or inability
to complete ooplasmic maturation.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>11369606</pmid><doi>10.1095/biolreprod64.6.1761</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2001-06, Vol.64 (6), p.1761-1768 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete |
| subjects | Aging Animals Biological and medical sciences Blastocyst - physiology Blotting, Western Calcium Channels - analysis Cattle - growth & development Cleavage Stage, Ovum Cytoplasm - physiology Female Fundamental and applied biological sciences. Psychology Inositol 1,4,5-Trisphosphate Receptors Mammalian female genital system Maturation-Promoting Factor - analysis Mitogen-Activated Protein Kinases - analysis Morphology. Physiology Myelin Basic Protein - metabolism Nuclear Transfer Techniques Oocytes - chemistry Oocytes - physiology Oocytes - ultrastructure Phosphorylation Protein Kinases - metabolism Receptors, Cytoplasmic and Nuclear - analysis Sexual Maturation Vertebrates: reproduction |
| title | Biochemical and Developmental Evidence That Ooplasmic Maturation of Prepubertal Bovine Oocytes Is Compromised |
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