Contraction kinetics of isolated human myometrium during menstrual cycle and pregnancy
Objective To investigate the interaction between actin and myosin in the myometrium by studying the contraction kinetics of isolated samples of human myometrium. Design Experimental and observational cross‐sectional study. Setting Eppendorf University Hospital, Hamburg. Samples Myometrium samples we...
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| creator | Tchirikov, Mikhail Peiper, Ulrich Schröder, Hobe J. |
| description | Objective
To investigate the interaction between actin and myosin in the myometrium by studying the contraction kinetics of isolated samples of human myometrium.
Design
Experimental and observational cross‐sectional study.
Setting
Eppendorf University Hospital, Hamburg.
Samples
Myometrium samples were taken from women in the follicular phase (n= 6) or luteal phase (n= 6) of the menstrual cycle and during pregnancy at term (n= 25).
Methods
The frequency, extent and rate of force development were determined in spontaneously active myometrial preparations. From a resting force of 2 mN, sustained tonic contractions were induced by K+‐depolarisation (124 mM), or by protein kinase C activation (19.9 μM indolactam). The steady force was reversibly interrupted by rapid length changes (100 Hz sinus vibrations lasting 1 s, 5% of muscle length). Extent (steady plateau), as well as rate of force increase after cessation of vibrations, were derived from bi‐exponential functions fitted to the time course of force recovery.
Results
Frequency of spontaneous contractions was higher in the follicular phase [mean (SD) 18.3 contractions/hour (1.0)] than in the luteal phase [13.4 contractions/hour (8.1)] or in pregnancy at term [8.8 contractions/hour (7.6)]. During indolactam treatment, steady force in pregnancy at term was significantly increased [8.8 mN (4.0)], compared with the follicular phase [3.7 mN (0.9)]. Force recovery was distinctly slower in pregnancy at term during indolactam treatment [time constant 99.2 s (57.9); P < 0.005] than during K+‐depolarisation [time constant 29.1 s (5.9)], whereas in the follicular phase the rate of force recovery was faster with indolactam [16.8 s (7.1)] than with K+ depolarisation [24.4 s (5.9); P < 0.005].
Conclusions
The responses of human myometrium to contraction stimuli differ according to the reproductive state. Membrane depolarisation causes similar responses in all myometrial strips. In contrast, near term stimulation of protein kinase C generates a large tonic force and slow contraction kinetics, whereas early in the menstrual cycle contraction kinetics are fast. |
| doi_str_mv | 10.1111/j.1471-0528.2000.tb11580.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70873974</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70873974</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3982-a916d06f03f788bc584781126f16e3bedb7fc769791fae70b5da8e59ddd95e4e3</originalsourceid><addsrcrecordid>eNqVkEtv1DAQgK2Kij7gL1RWhbgl9SOxHSQOZdUHVaVegKvl2OPiJXEWOxHNvyfbXRWuncuMNN889CF0TklJl7hYl7SStCA1UyUjhJRjS2mtSPl0gI5fWm-ea1IQztQROsl5TQgVjPC36IgSUdVK8GP0YzXEMRk7hiHiXyHCGGzGg8chD50ZweGfU28i7uehhzGFqcduSiE-4h5iHtNkOmxn2wE20eFNgsdoop3foUNvugzv9_kUfb---ra6Le4fbr6uLu8LyxvFCtNQ4YjwhHupVGtrVUlFKROeCuAtuFZ6K0UjG-oNSNLWziioG-dcU0MF_BR93O3dpOH3BHnUfcgWus5EGKasJVGSN7JawE870KYh5wReb1LoTZo1JXprVa_1Vp3eqtNbq3pvVT8tw2f7K1Pbg_tvdKdxAT7sAZOt6XxaHIT8j2OsZkIs2Ocd9id0ML_iA_3l7kEw_hd8I5Vh</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70873974</pqid></control><display><type>article</type><title>Contraction kinetics of isolated human myometrium during menstrual cycle and pregnancy</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><creator>Tchirikov, Mikhail ; Peiper, Ulrich ; Schröder, Hobe J.</creator><creatorcontrib>Tchirikov, Mikhail ; Peiper, Ulrich ; Schröder, Hobe J.</creatorcontrib><description>Objective
To investigate the interaction between actin and myosin in the myometrium by studying the contraction kinetics of isolated samples of human myometrium.
Design
Experimental and observational cross‐sectional study.
Setting
Eppendorf University Hospital, Hamburg.
Samples
Myometrium samples were taken from women in the follicular phase (n= 6) or luteal phase (n= 6) of the menstrual cycle and during pregnancy at term (n= 25).
Methods
The frequency, extent and rate of force development were determined in spontaneously active myometrial preparations. From a resting force of 2 mN, sustained tonic contractions were induced by K+‐depolarisation (124 mM), or by protein kinase C activation (19.9 μM indolactam). The steady force was reversibly interrupted by rapid length changes (100 Hz sinus vibrations lasting 1 s, 5% of muscle length). Extent (steady plateau), as well as rate of force increase after cessation of vibrations, were derived from bi‐exponential functions fitted to the time course of force recovery.
Results
Frequency of spontaneous contractions was higher in the follicular phase [mean (SD) 18.3 contractions/hour (1.0)] than in the luteal phase [13.4 contractions/hour (8.1)] or in pregnancy at term [8.8 contractions/hour (7.6)]. During indolactam treatment, steady force in pregnancy at term was significantly increased [8.8 mN (4.0)], compared with the follicular phase [3.7 mN (0.9)]. Force recovery was distinctly slower in pregnancy at term during indolactam treatment [time constant 99.2 s (57.9); P < 0.005] than during K+‐depolarisation [time constant 29.1 s (5.9)], whereas in the follicular phase the rate of force recovery was faster with indolactam [16.8 s (7.1)] than with K+ depolarisation [24.4 s (5.9); P < 0.005].
Conclusions
The responses of human myometrium to contraction stimuli differ according to the reproductive state. Membrane depolarisation causes similar responses in all myometrial strips. In contrast, near term stimulation of protein kinase C generates a large tonic force and slow contraction kinetics, whereas early in the menstrual cycle contraction kinetics are fast.</description><identifier>ISSN: 1470-0328</identifier><identifier>ISSN: 0306-5456</identifier><identifier>EISSN: 1471-0528</identifier><identifier>EISSN: 1365-215X</identifier><identifier>DOI: 10.1111/j.1471-0528.2000.tb11580.x</identifier><identifier>PMID: 10645863</identifier><identifier>CODEN: BJOGAS</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Cross-Sectional Studies ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Mammalian female genital system ; Menstrual Cycle - physiology ; Morphology. Physiology ; Mother. Fetoplacental unit. Mammary gland. Milk ; Myometrium - drug effects ; Myometrium - physiology ; Pregnancy - physiology ; Pregnancy. Parturition. Lactation ; Uterine Contraction - drug effects ; Uterine Contraction - physiology ; Vertebrates: reproduction</subject><ispartof>BJOG : an international journal of obstetrics and gynaecology, 2000-01, Vol.107 (1), p.62-67</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3982-a916d06f03f788bc584781126f16e3bedb7fc769791fae70b5da8e59ddd95e4e3</citedby><cites>FETCH-LOGICAL-c3982-a916d06f03f788bc584781126f16e3bedb7fc769791fae70b5da8e59ddd95e4e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-0528.2000.tb11580.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-0528.2000.tb11580.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4009,27902,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1225266$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10645863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tchirikov, Mikhail</creatorcontrib><creatorcontrib>Peiper, Ulrich</creatorcontrib><creatorcontrib>Schröder, Hobe J.</creatorcontrib><title>Contraction kinetics of isolated human myometrium during menstrual cycle and pregnancy</title><title>BJOG : an international journal of obstetrics and gynaecology</title><addtitle>BJOG</addtitle><description>Objective
To investigate the interaction between actin and myosin in the myometrium by studying the contraction kinetics of isolated samples of human myometrium.
Design
Experimental and observational cross‐sectional study.
Setting
Eppendorf University Hospital, Hamburg.
Samples
Myometrium samples were taken from women in the follicular phase (n= 6) or luteal phase (n= 6) of the menstrual cycle and during pregnancy at term (n= 25).
Methods
The frequency, extent and rate of force development were determined in spontaneously active myometrial preparations. From a resting force of 2 mN, sustained tonic contractions were induced by K+‐depolarisation (124 mM), or by protein kinase C activation (19.9 μM indolactam). The steady force was reversibly interrupted by rapid length changes (100 Hz sinus vibrations lasting 1 s, 5% of muscle length). Extent (steady plateau), as well as rate of force increase after cessation of vibrations, were derived from bi‐exponential functions fitted to the time course of force recovery.
Results
Frequency of spontaneous contractions was higher in the follicular phase [mean (SD) 18.3 contractions/hour (1.0)] than in the luteal phase [13.4 contractions/hour (8.1)] or in pregnancy at term [8.8 contractions/hour (7.6)]. During indolactam treatment, steady force in pregnancy at term was significantly increased [8.8 mN (4.0)], compared with the follicular phase [3.7 mN (0.9)]. Force recovery was distinctly slower in pregnancy at term during indolactam treatment [time constant 99.2 s (57.9); P < 0.005] than during K+‐depolarisation [time constant 29.1 s (5.9)], whereas in the follicular phase the rate of force recovery was faster with indolactam [16.8 s (7.1)] than with K+ depolarisation [24.4 s (5.9); P < 0.005].
Conclusions
The responses of human myometrium to contraction stimuli differ according to the reproductive state. Membrane depolarisation causes similar responses in all myometrial strips. In contrast, near term stimulation of protein kinase C generates a large tonic force and slow contraction kinetics, whereas early in the menstrual cycle contraction kinetics are fast.</description><subject>Biological and medical sciences</subject><subject>Cross-Sectional Studies</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Mammalian female genital system</subject><subject>Menstrual Cycle - physiology</subject><subject>Morphology. Physiology</subject><subject>Mother. Fetoplacental unit. Mammary gland. Milk</subject><subject>Myometrium - drug effects</subject><subject>Myometrium - physiology</subject><subject>Pregnancy - physiology</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Uterine Contraction - drug effects</subject><subject>Uterine Contraction - physiology</subject><subject>Vertebrates: reproduction</subject><issn>1470-0328</issn><issn>0306-5456</issn><issn>1471-0528</issn><issn>1365-215X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkEtv1DAQgK2Kij7gL1RWhbgl9SOxHSQOZdUHVaVegKvl2OPiJXEWOxHNvyfbXRWuncuMNN889CF0TklJl7hYl7SStCA1UyUjhJRjS2mtSPl0gI5fWm-ea1IQztQROsl5TQgVjPC36IgSUdVK8GP0YzXEMRk7hiHiXyHCGGzGg8chD50ZweGfU28i7uehhzGFqcduSiE-4h5iHtNkOmxn2wE20eFNgsdoop3foUNvugzv9_kUfb---ra6Le4fbr6uLu8LyxvFCtNQ4YjwhHupVGtrVUlFKROeCuAtuFZ6K0UjG-oNSNLWziioG-dcU0MF_BR93O3dpOH3BHnUfcgWus5EGKasJVGSN7JawE870KYh5wReb1LoTZo1JXprVa_1Vp3eqtNbq3pvVT8tw2f7K1Pbg_tvdKdxAT7sAZOt6XxaHIT8j2OsZkIs2Ocd9id0ML_iA_3l7kEw_hd8I5Vh</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>Tchirikov, Mikhail</creator><creator>Peiper, Ulrich</creator><creator>Schröder, Hobe J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>Contraction kinetics of isolated human myometrium during menstrual cycle and pregnancy</title><author>Tchirikov, Mikhail ; Peiper, Ulrich ; Schröder, Hobe J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3982-a916d06f03f788bc584781126f16e3bedb7fc769791fae70b5da8e59ddd95e4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biological and medical sciences</topic><topic>Cross-Sectional Studies</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Mammalian female genital system</topic><topic>Menstrual Cycle - physiology</topic><topic>Morphology. Physiology</topic><topic>Mother. Fetoplacental unit. Mammary gland. Milk</topic><topic>Myometrium - drug effects</topic><topic>Myometrium - physiology</topic><topic>Pregnancy - physiology</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Uterine Contraction - drug effects</topic><topic>Uterine Contraction - physiology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tchirikov, Mikhail</creatorcontrib><creatorcontrib>Peiper, Ulrich</creatorcontrib><creatorcontrib>Schröder, Hobe J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>BJOG : an international journal of obstetrics and gynaecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tchirikov, Mikhail</au><au>Peiper, Ulrich</au><au>Schröder, Hobe J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contraction kinetics of isolated human myometrium during menstrual cycle and pregnancy</atitle><jtitle>BJOG : an international journal of obstetrics and gynaecology</jtitle><addtitle>BJOG</addtitle><date>2000-01</date><risdate>2000</risdate><volume>107</volume><issue>1</issue><spage>62</spage><epage>67</epage><pages>62-67</pages><issn>1470-0328</issn><issn>0306-5456</issn><eissn>1471-0528</eissn><eissn>1365-215X</eissn><coden>BJOGAS</coden><abstract>Objective
To investigate the interaction between actin and myosin in the myometrium by studying the contraction kinetics of isolated samples of human myometrium.
Design
Experimental and observational cross‐sectional study.
Setting
Eppendorf University Hospital, Hamburg.
Samples
Myometrium samples were taken from women in the follicular phase (n= 6) or luteal phase (n= 6) of the menstrual cycle and during pregnancy at term (n= 25).
Methods
The frequency, extent and rate of force development were determined in spontaneously active myometrial preparations. From a resting force of 2 mN, sustained tonic contractions were induced by K+‐depolarisation (124 mM), or by protein kinase C activation (19.9 μM indolactam). The steady force was reversibly interrupted by rapid length changes (100 Hz sinus vibrations lasting 1 s, 5% of muscle length). Extent (steady plateau), as well as rate of force increase after cessation of vibrations, were derived from bi‐exponential functions fitted to the time course of force recovery.
Results
Frequency of spontaneous contractions was higher in the follicular phase [mean (SD) 18.3 contractions/hour (1.0)] than in the luteal phase [13.4 contractions/hour (8.1)] or in pregnancy at term [8.8 contractions/hour (7.6)]. During indolactam treatment, steady force in pregnancy at term was significantly increased [8.8 mN (4.0)], compared with the follicular phase [3.7 mN (0.9)]. Force recovery was distinctly slower in pregnancy at term during indolactam treatment [time constant 99.2 s (57.9); P < 0.005] than during K+‐depolarisation [time constant 29.1 s (5.9)], whereas in the follicular phase the rate of force recovery was faster with indolactam [16.8 s (7.1)] than with K+ depolarisation [24.4 s (5.9); P < 0.005].
Conclusions
The responses of human myometrium to contraction stimuli differ according to the reproductive state. Membrane depolarisation causes similar responses in all myometrial strips. In contrast, near term stimulation of protein kinase C generates a large tonic force and slow contraction kinetics, whereas early in the menstrual cycle contraction kinetics are fast.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10645863</pmid><doi>10.1111/j.1471-0528.2000.tb11580.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | BJOG : an international journal of obstetrics and gynaecology, 2000-01, Vol.107 (1), p.62-67 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Cross-Sectional Studies Female Fundamental and applied biological sciences. Psychology Humans Mammalian female genital system Menstrual Cycle - physiology Morphology. Physiology Mother. Fetoplacental unit. Mammary gland. Milk Myometrium - drug effects Myometrium - physiology Pregnancy - physiology Pregnancy. Parturition. Lactation Uterine Contraction - drug effects Uterine Contraction - physiology Vertebrates: reproduction |
| title | Contraction kinetics of isolated human myometrium during menstrual cycle and pregnancy |
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