Synthesis and Hydrolysis by Cysteine and Serine Proteases of Short Internally Quenched Fluorogenic Peptides

We developed sensitive substrates for cysteine proteases and specific substrates for serine proteases based on short internally quenched fluorescent peptides, Abz-F-R-X-EDDnp, where Abz (ortho-aminobenzoic acid) is the fluorescent donor, EDDnp [N-(ethylenediamine)-2,4-dinitrophenyl amide] is the flu...

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Veröffentlicht in:	Analytical biochemistry 2001-06, Vol.293 (1), p.71-77
Hauptverfasser:	Melo, Robson L., Alves, Lira C., Del Nery, Elaine, Juliano, Luiz, Juliano, Maria A.
Format:	Artikel
Sprache:	eng
Schlagworte:	cathepsin B Cathepsin B - metabolism Cathepsin L Cathepsins - metabolism Cysteine Endopeptidases Endopeptidases Factor X - metabolism Fluorescent Dyes - metabolism Humans Hydrogen-Ion Concentration Hydrolysis kallikrein Kallikreins - metabolism Liver - enzymology papain Papain - metabolism Peptide Fragments - chemical synthesis Substrate Specificity Thrombin - metabolism trypsin Trypsin - metabolism
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