Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors

Although the mouse spleen dendritic cell (DC) is perhaps the most intensively studied DC type, little has been published concerning its human equivalent. In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR+CD3−1...

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Veröffentlicht in:Blood 2001-06, Vol.97 (11), p.3470-3477
Hauptverfasser: McIlroy, Dorian, Troadec, Christelle, Grassi, Fernanda, Samri, Assia, Barrou, Benoı̂t, Autran, Brigitte, Debré, Patrice, Feuillard, Jean, Hosmalin, Anne
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container_end_page 3477
container_issue 11
container_start_page 3470
container_title Blood
container_volume 97
creator McIlroy, Dorian
Troadec, Christelle
Grassi, Fernanda
Samri, Assia
Barrou, Benoı̂t
Autran, Brigitte
Debré, Patrice
Feuillard, Jean
Hosmalin, Anne
description Although the mouse spleen dendritic cell (DC) is perhaps the most intensively studied DC type, little has been published concerning its human equivalent. In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR+CD3−14−16−19− human spleen DC. Spleens from organ donors with different clinical histories were used. Most (81% ± 9%; n = 14) spleen DCs expressed high levels of the integrin CD11c. CD11c+ DCs were distributed in 3 distinct regions—the peri-arteriolar T-cell zones, the B-cell zones, and the marginal zone, where they formed a ring of cells surrounding the white pulp, just inside a ring of CD14+ red pulp macrophages, apparently more regularly organized than the previously described marginating DC population in the mouse spleen. The T-cell zones contained CD86+ DCs, among which a subpopulation expressed CD83. These mature/activated CD86+DCs represented a minority (12% ± 8%) of total spleen DCs in most organ donors: most spleen DCs are immature. In 3 of 18 (17%) donors, however, most (54%-81%) of spleen DCs were CD86+, suggesting that in vivo DC activation had occurred. In one donor, a radical shift in DC distribution from the marginal zone to the T-cell zones was also observed. This activation of spleen DCs in vivo was reminiscent of the effects of experimental microbial product injection in mice, and it seemed to correlate with bacterial infection or multiple trauma.
doi_str_mv 10.1182/blood.V97.11.3470
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In 3 of 18 (17%) donors, however, most (54%-81%) of spleen DCs were CD86+, suggesting that in vivo DC activation had occurred. In one donor, a radical shift in DC distribution from the marginal zone to the T-cell zones was also observed. This activation of spleen DCs in vivo was reminiscent of the effects of experimental microbial product injection in mice, and it seemed to correlate with bacterial infection or multiple trauma.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>11369639</pmid><doi>10.1182/blood.V97.11.3470</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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subjects Adult
Analysis of the immune response. Humoral and cellular immunity
Antigens, CD - analysis
B7-2 Antigen
Biological and medical sciences
CD11 Antigens - analysis
CD3 Complex - analysis
CD83 Antigen
Dendritic Cells - cytology
Dendritic Cells - immunology
Female
Flow Cytometry
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Fundamental immunology
HLA-DR Antigens - analysis
Humans
Immunobiology
Immunoglobulins - analysis
Immunophenotyping
Interleukin-12 - metabolism
Lipopolysaccharide Receptors - analysis
Male
Membrane Glycoproteins - analysis
Middle Aged
Organs and cells involved in the immune response
Phenotype
Spleen - cytology
Tissue Donors
title Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors
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