Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors
Although the mouse spleen dendritic cell (DC) is perhaps the most intensively studied DC type, little has been published concerning its human equivalent. In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR+CD3−1...
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description | Although the mouse spleen dendritic cell (DC) is perhaps the most intensively studied DC type, little has been published concerning its human equivalent. In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR+CD3−14−16−19− human spleen DC. Spleens from organ donors with different clinical histories were used. Most (81% ± 9%; n = 14) spleen DCs expressed high levels of the integrin CD11c. CD11c+ DCs were distributed in 3 distinct regions—the peri-arteriolar T-cell zones, the B-cell zones, and the marginal zone, where they formed a ring of cells surrounding the white pulp, just inside a ring of CD14+ red pulp macrophages, apparently more regularly organized than the previously described marginating DC population in the mouse spleen. The T-cell zones contained CD86+ DCs, among which a subpopulation expressed CD83. These mature/activated CD86+DCs represented a minority (12% ± 8%) of total spleen DCs in most organ donors: most spleen DCs are immature. In 3 of 18 (17%) donors, however, most (54%-81%) of spleen DCs were CD86+, suggesting that in vivo DC activation had occurred. In one donor, a radical shift in DC distribution from the marginal zone to the T-cell zones was also observed. This activation of spleen DCs in vivo was reminiscent of the effects of experimental microbial product injection in mice, and it seemed to correlate with bacterial infection or multiple trauma. |
doi_str_mv | 10.1182/blood.V97.11.3470 |
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In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR+CD3−14−16−19− human spleen DC. Spleens from organ donors with different clinical histories were used. Most (81% ± 9%; n = 14) spleen DCs expressed high levels of the integrin CD11c. CD11c+ DCs were distributed in 3 distinct regions—the peri-arteriolar T-cell zones, the B-cell zones, and the marginal zone, where they formed a ring of cells surrounding the white pulp, just inside a ring of CD14+ red pulp macrophages, apparently more regularly organized than the previously described marginating DC population in the mouse spleen. The T-cell zones contained CD86+ DCs, among which a subpopulation expressed CD83. These mature/activated CD86+DCs represented a minority (12% ± 8%) of total spleen DCs in most organ donors: most spleen DCs are immature. In 3 of 18 (17%) donors, however, most (54%-81%) of spleen DCs were CD86+, suggesting that in vivo DC activation had occurred. In one donor, a radical shift in DC distribution from the marginal zone to the T-cell zones was also observed. This activation of spleen DCs in vivo was reminiscent of the effects of experimental microbial product injection in mice, and it seemed to correlate with bacterial infection or multiple trauma.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V97.11.3470</identifier><identifier>PMID: 11369639</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Adult ; Analysis of the immune response. 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In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR+CD3−14−16−19− human spleen DC. Spleens from organ donors with different clinical histories were used. Most (81% ± 9%; n = 14) spleen DCs expressed high levels of the integrin CD11c. CD11c+ DCs were distributed in 3 distinct regions—the peri-arteriolar T-cell zones, the B-cell zones, and the marginal zone, where they formed a ring of cells surrounding the white pulp, just inside a ring of CD14+ red pulp macrophages, apparently more regularly organized than the previously described marginating DC population in the mouse spleen. The T-cell zones contained CD86+ DCs, among which a subpopulation expressed CD83. These mature/activated CD86+DCs represented a minority (12% ± 8%) of total spleen DCs in most organ donors: most spleen DCs are immature. In 3 of 18 (17%) donors, however, most (54%-81%) of spleen DCs were CD86+, suggesting that in vivo DC activation had occurred. In one donor, a radical shift in DC distribution from the marginal zone to the T-cell zones was also observed. This activation of spleen DCs in vivo was reminiscent of the effects of experimental microbial product injection in mice, and it seemed to correlate with bacterial infection or multiple trauma.</description><subject>Adult</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Antigens, CD - analysis</subject><subject>B7-2 Antigen</subject><subject>Biological and medical sciences</subject><subject>CD11 Antigens - analysis</subject><subject>CD3 Complex - analysis</subject><subject>CD83 Antigen</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - immunology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>HLA-DR Antigens - analysis</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Immunoglobulins - analysis</subject><subject>Immunophenotyping</subject><subject>Interleukin-12 - metabolism</subject><subject>Lipopolysaccharide Receptors - analysis</subject><subject>Male</subject><subject>Membrane Glycoproteins - analysis</subject><subject>Middle Aged</subject><subject>Organs and cells involved in the immune response</subject><subject>Phenotype</subject><subject>Spleen - cytology</subject><subject>Tissue Donors</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2OFCEUhYnROO3oA7gxLIy7aqGAqiauzGTUSSZxo24JP5cZTDWUQFUyr-BTS013oitX5JLvnPtzEHpNyZ7SQ__eTCm5_Q85tnLP-EieoB0V_aEjpCdP0Y4QMnRcjvQCvSjlJyGUs148RxeUskEOTO7Q75u4QqnhTteQIk4e3y9HHXGZJ4CIHUSXQw0WW5gmPN9DTPVhBqyjwy6UmoNZHpUZVtBTwbCGJrKwWYWI17AmrG0N66lB-9K4LKZA3YiU71ozl2LK5SV65psDvDq_l-j7p-tvV1-626-fb64-3nZWMFE7Luw4cOnY6KQXxlvW8wO1XlImaS_AgDF-8JY4sIYZ6ZnknNNx9EIax3t2id6dfOecfi1teXUMZVtPR0hLUSM5jJQL1kB6Am1OpWTwas7hqPODokRtAajHAFQLoJVqC6Bp3pzNF3ME91dxvngD3p4BXayefNbRhvKPsyCCbz4fThi0S6wBsio2bHd1IYOtyqXwnyn-AL0rpxE</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>McIlroy, Dorian</creator><creator>Troadec, Christelle</creator><creator>Grassi, Fernanda</creator><creator>Samri, Assia</creator><creator>Barrou, Benoı̂t</creator><creator>Autran, Brigitte</creator><creator>Debré, Patrice</creator><creator>Feuillard, Jean</creator><creator>Hosmalin, Anne</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors</title><author>McIlroy, Dorian ; Troadec, Christelle ; Grassi, Fernanda ; Samri, Assia ; Barrou, Benoı̂t ; Autran, Brigitte ; Debré, Patrice ; Feuillard, Jean ; Hosmalin, Anne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-45c7649d37d9f5bfc32481cf9139125ebebbf6fc0decb3b9f39444177f59bd423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Antigens, CD - analysis</topic><topic>B7-2 Antigen</topic><topic>Biological and medical sciences</topic><topic>CD11 Antigens - analysis</topic><topic>CD3 Complex - analysis</topic><topic>CD83 Antigen</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>HLA-DR Antigens - analysis</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Immunoglobulins - analysis</topic><topic>Immunophenotyping</topic><topic>Interleukin-12 - metabolism</topic><topic>Lipopolysaccharide Receptors - analysis</topic><topic>Male</topic><topic>Membrane Glycoproteins - analysis</topic><topic>Middle Aged</topic><topic>Organs and cells involved in the immune response</topic><topic>Phenotype</topic><topic>Spleen - cytology</topic><topic>Tissue Donors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McIlroy, Dorian</creatorcontrib><creatorcontrib>Troadec, Christelle</creatorcontrib><creatorcontrib>Grassi, Fernanda</creatorcontrib><creatorcontrib>Samri, Assia</creatorcontrib><creatorcontrib>Barrou, Benoı̂t</creatorcontrib><creatorcontrib>Autran, Brigitte</creatorcontrib><creatorcontrib>Debré, Patrice</creatorcontrib><creatorcontrib>Feuillard, Jean</creatorcontrib><creatorcontrib>Hosmalin, Anne</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McIlroy, Dorian</au><au>Troadec, Christelle</au><au>Grassi, Fernanda</au><au>Samri, Assia</au><au>Barrou, Benoı̂t</au><au>Autran, Brigitte</au><au>Debré, Patrice</au><au>Feuillard, Jean</au><au>Hosmalin, Anne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>97</volume><issue>11</issue><spage>3470</spage><epage>3477</epage><pages>3470-3477</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>Although the mouse spleen dendritic cell (DC) is perhaps the most intensively studied DC type, little has been published concerning its human equivalent. In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR+CD3−14−16−19− human spleen DC. Spleens from organ donors with different clinical histories were used. Most (81% ± 9%; n = 14) spleen DCs expressed high levels of the integrin CD11c. CD11c+ DCs were distributed in 3 distinct regions—the peri-arteriolar T-cell zones, the B-cell zones, and the marginal zone, where they formed a ring of cells surrounding the white pulp, just inside a ring of CD14+ red pulp macrophages, apparently more regularly organized than the previously described marginating DC population in the mouse spleen. The T-cell zones contained CD86+ DCs, among which a subpopulation expressed CD83. These mature/activated CD86+DCs represented a minority (12% ± 8%) of total spleen DCs in most organ donors: most spleen DCs are immature. In 3 of 18 (17%) donors, however, most (54%-81%) of spleen DCs were CD86+, suggesting that in vivo DC activation had occurred. In one donor, a radical shift in DC distribution from the marginal zone to the T-cell zones was also observed. This activation of spleen DCs in vivo was reminiscent of the effects of experimental microbial product injection in mice, and it seemed to correlate with bacterial infection or multiple trauma.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>11369639</pmid><doi>10.1182/blood.V97.11.3470</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Analysis of the immune response. Humoral and cellular immunity Antigens, CD - analysis B7-2 Antigen Biological and medical sciences CD11 Antigens - analysis CD3 Complex - analysis CD83 Antigen Dendritic Cells - cytology Dendritic Cells - immunology Female Flow Cytometry Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology HLA-DR Antigens - analysis Humans Immunobiology Immunoglobulins - analysis Immunophenotyping Interleukin-12 - metabolism Lipopolysaccharide Receptors - analysis Male Membrane Glycoproteins - analysis Middle Aged Organs and cells involved in the immune response Phenotype Spleen - cytology Tissue Donors |
title | Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors |
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